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Citations for Agilent :
1101 - 1150 of 1553 citations for Mouse KIF26B shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... The EnVision anti-mouse system was used for visualization according to the manufacturer’s instructions (Agilent).
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bioRxiv - Cancer Biology 2024Quote: ... followed by polyclonal goat anti-mouse/rabbit immunoglobulins horseradish peroxidase (HRP)-conjugated secondary antibodies (Dako) diluted 1:1000 for 1 hour at room temperature ...
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bioRxiv - Immunology 2024Quote: ... staining was visualized by the anti-mouse EnVision+® System-HRP (DAB) (DakoCytomation, Glostrup, Denmark). Human testis tissue was used as positive control tissue ...
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bioRxiv - Microbiology 2023Quote: ... followed by horseradish peroxidase (HRP-) conjugated goat anti-mouse polyclonal antibody (DAKO, Carpinteria, CA, USA) (1:2000 in PBS-Tween 20) ...
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bioRxiv - Molecular Biology 2021Quote: ... The T695A and T656A mutations were generated in WT-YME1L1 plasmid via QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA) and confirmed by sequencing ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were co-transfected with 100ng pCAG-Lgr6 overexpression plasmid (pLgr6) or pBluescript II KS+ (pBKS) (Agilent Technologies, Santa Clara, CA, USA) as a control ...
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bioRxiv - Molecular Biology 2020Quote: ... EcoRV and SacI restriction sites that flanked the B3 domain were introduced into the VP1-TOPO plasmid by site-directed mutagenesis (Agilent Technologies, CA); 2 ...
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bioRxiv - Cell Biology 2020Quote: pcDNA3 + mNICD W1758A: this plasmid was generated by PCR-based mutagenesis using the QuikChange Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3 + mNICD as a template and the primers W1758A F and W1758A R.
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bioRxiv - Biochemistry 2021Quote: Plasmids encoding AlgE7 variants (Table 2) were constructed based on wild type AlgE7 (plasmid pBG27) (12) using the QuikChange™ Site-directed Mutagenesis Kit (Stratagene/Agilent) or Q5 site directed mutagenesis (New England Biolabs) ...
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bioRxiv - Microbiology 2020Quote: ... Lens was introduced to the leader region of the MC-II through MC-VI and MC-V-dotO arrays in our donor plasmids by quickchange PCR with Pfu Turbo Polymerase (Agilent #600250-52). boxA(−58 ...
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bioRxiv - Neuroscience 2022Quote: Co-transfection of pAAV-CAG-DIO-(hCAR)off-(GtACR2Kv2.1-MuGFP)on-W3SL with pDPI and pDPII plasmids (Grimm et al., 2003) into AAV293 cells (Agilent Technologies, CA, USA) preceded harvesting and iodixanol gradient purification (as described by (Zolotukhin et al. ...
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bioRxiv - Cell Biology 2023Quote: pLV-CMV-IRIS-PURO-c-Src-mScarlet plasmid was used to the generation of Y527F and Y527F/K295R mutants via QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies, 200523) according to manufacturer’s protocol using the primers listed in Table 1.
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bioRxiv - Plant Biology 2023Quote: ... Site-directed mutants of KflaHY2 were generated in the pET28a_KflaHY2 plasmid using the QuikChange® Lightning kit (Agilent, Santa Clara, California, USA) employing primers listed in Table S1 ...
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bioRxiv - Cell Biology 2024Quote: Protein expression was initiated by transforming ∼80 ng of each DNA plasmid into 50 µL of XL-1 Chemically Supercompetent Cells (Agilent Technologies, 200236) at 42 °C for 30 seconds followed by 5 minutes of incubation on ice ...
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bioRxiv - Neuroscience 2023Quote: ... the four 130 bp α-unit DNA fragments were amplified from each α-unit plasmid using the Herculase II Fusion DNA polymerase (Agilent) and oJS2581 and oJS2582 primers77 ...
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bioRxiv - Genetics 2023Quote: ... 15 PCR-2 reactions were completed using Oligo 45 paired with Oligos 47-61 to amplify from YCp50-WT_PKR plasmid using Herculase II polymerase (Agilent Technologies Cat#600677) using cycling conditions for vector targets >10 kilo-base pairs ...
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bioRxiv - Cell Biology 2023Quote: ... GYF motif mutant GIGYF1 (GIGYF1 GYF Mut; G502A, Y503A, F504A) plasmids were created using the QuikChange II Site-Directed mutagenesis kit (Agilent Technologies, 200523). The chimeric constructs were synthesized by GenScript (Piscataway ...
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bioRxiv - Systems Biology 2020Quote: ... mouse and naked mole rat cells were plated onto a Seahorse XF96 Cell Culture Microplate (Agilent) at a cell density of 105,000 and 80,150 cells per well respectively to allow the cells to be 100% confluent ...
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bioRxiv - Immunology 2021Quote: ... the membranes were incubated with either HRP-conjugated goat anti-mouse (250 ng/mL; # P0447, Agilent) or HRP-conjugated goat anti-rabbit immunoglobulin (62.5 ng/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... The secondary antibodies were horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG (1:5000, Dako, Japan) and HRP goat anti-rat IgG antibody (Biolegend ...
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bioRxiv - Microbiology 2019Quote: ... bound Igs were detected with Fluorescein isothiocyanate (FITC)-conjugated rabbit anti-mouse Ig antibody (Dako Cytomation). To quantify total IgG levels ...
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bioRxiv - Developmental Biology 2021Quote: ... The cRNAs were hybridized on a SurePrint G3 Mouse Gene Expression 8 × 60K Microarray (Agilent Technologies), and fluorescence signals were detected using the SureScan Microarray Scanner (Agilent Technologies) ...
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bioRxiv - Genomics 2020Quote: ... LECs were washed twice in DPBS and stained with mouse anti-human Ki-67 antibody (Dako) diluted 1:800 in FACS buffer (DPBS with 1mM EDTA and 2% FBS ...
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bioRxiv - Neuroscience 2019Quote: ... GFAP immunohistochemistry for astrocytes (rabbit anti–mouse GFAP polyclonal antibody 1:13000 for 24 min; DAKO) and IBA-1 for microglia (rabbit polyclonal antibody 1:1000 for 24 min ...
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bioRxiv - Immunology 2019Quote: ... Diluted monoclonal mouse anti-Ki-67 antibody (TEC-3 antibody [Dako Code M7249]; Dako, Glostrup, Denmark) was then applied and slides incubated overnight at room temperature in a humidified chamber ...
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bioRxiv - Immunology 2019Quote: ... Diluted monoclonal mouse anti-Ki-67 antibody (TEC-3 antibody [Dako Code M7249]; Dako, Glostrup, Denmark) was then applied and slides incubated overnight at room temperature in a humidified chamber ...
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bioRxiv - Immunology 2019Quote: ... Calibration beads were stained with F(ab’)2 FITC-Conjugated Goat Anti-Mouse immunoglobulins (Dako, F0479) at 1:50 dilution for 1 hr at 4°C ...
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bioRxiv - Microbiology 2020Quote: ... Cells were then intracellularly stained with 4G2 mAbs followed by rabbit anti-mouse Igs FITC (Dako). The cells were fixed with 1% formaldehyde and analyzed on BD LSRFortessa ...
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bioRxiv - Cancer Biology 2021Quote: Whole-exome libraries for all samples were generated using the SureSelect XT Mouse All Exon (Agilent) target enrichment kit with 100 bp paired-end sequencing on the Illumina HiSeq 4000 platform ...
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bioRxiv - Cancer Biology 2022Quote: We performed immunochemistry assays using mouse anti-human ki67 antibody (M7240, DAKO, 1/200 at pH9) in a series of paraffin-embedded tissue blocks of HGSOC ...
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bioRxiv - Biochemistry 2022Quote: ... and aSN (primary antibody: anti-Syn-1, BD Transduction Laboratory, secondary antibody: anti-mouse-HRP, Dako). The interaction between endogenous aSN and the endogenous PMCA was studied in the extracts from C57BL/6 mice as described above.
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bioRxiv - Neuroscience 2022Quote: ... 29] for astrocytes (rabbit anti-GFAP 1:500, Dako, mouse anti-α-tubulin 1:500, Sigma) and microglia (rabbit anti-Iba1 ...
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bioRxiv - Developmental Biology 2022Quote: Primary antibodies used for staining of hBVOs were mouse anti-hCD31 (dilution 1:100, Dako, M0823), rabbit anti-hPDGFR-β (dilution 1:100 ...
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bioRxiv - Neuroscience 2020Quote: ... The microarray was performed using the SuperPrint G3 Mouse GE 8×60K Microarray Kit (Agilent, #G4852A) and a DNA microarray scanner ...
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bioRxiv - Immunology 2021Quote: ... Next day sections were stained using a goat anti-mouse-horseradish peroxidase (HRP)-conjugated antibody (Dako) for BRCA2 and goat anti-rat-HRP-conjugated antibody (Southern Biotech ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation with a horseradish peroxidase-labeled anti-mouse IgG (65 ng/ml; Dako, Denmark) diluted in 5% skimmed milk / TBS-Tween (0.1%) ...
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bioRxiv - Immunology 2020Quote: ... membranes were incubated with secondary Abs (goat anti-rabbit HRP or rabbit anti-mouse HRP; DAKO, Agilent Technologies ...
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bioRxiv - Cell Biology 2020Quote: ... the sections were exposed to anti-mouse DAKO EnVision+ System-HRP Labeled Polymer (DAKO, Glostrup, Denmark) for 35 minutes at room temperature ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Horseradish peroxidase (HRP)-conjugated goat anti-mouse IgG polyclonal antibody was purchased from Dako (Glostrup, Denmark). Super RX X-ray films were obtained from Fujifilm (Tokyo ...
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bioRxiv - Microbiology 2021Quote: ... After a 30 min incubation with the secondary rabbit-anti mouse IgG antibody (1:25) (Dako) followed a 30 min incubation step with alkaline-phosphatase-anti-alkaline-phosphatase (1:40 ...
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bioRxiv - Biochemistry 2021Quote: ... Total RNA was examined using the SurePrint G3 Mouse GE 8×60K Microarray (Agilent Technologies, USA.). Data were quantified using the Agilent Feature Extraction software (Agilent Technologies ...
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bioRxiv - Cancer Biology 2021Quote: ... 1 hour incubation at room temperature with secondary antibodies (HRP-conjugated anti-mouse secondary (Dako #P0447); HRP-conjugated anti-rabbit secondary (Dako #P0448) ...
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bioRxiv - Immunology 2021Quote: ... Secondary antibodies used for Western blotting in this study include: anti-Mouse IgG-HRP (Dako P0447), anti-Rabbit IgG-HRP (Bio-Rad 1706515) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated with 50 µl Horse Radish Peroxidase (HRP-) conjugated goat anti-mouse antibody (Dako) (1:2000 in PBS-Tween ...
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bioRxiv - Microbiology 2024Quote: ... the membrane was incubated in secondary antibody (anti-mouse HRP-linked secondary antibody (P0447, Agilent/Dako) diluted 1:10,000 ...
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bioRxiv - Microbiology 2024Quote: ... the membrane was incubated in secondary antibody (anti-mouse HRP-linked secondary antibody (P0447, Agilent/Dako) diluted 1:10,000 ...
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bioRxiv - Pathology 2024Quote: ... the sections were incubated with horseradish peroxidase (HRP) secondary mouse antibody (DAKO Envision kit cat# K4001) for 30 min and the staining was visualized using 3,3’-diaminobenzidine (DAB ...
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bioRxiv - Genetics 2023Quote: ... Secondary antibody incubation was performed in polyclonal rabbit anti mouse – HRP antibody 1:2000 (Dako, P0260) or polyclonal goat anti rabbit – HRP antibody 1:2000 (Dako ...
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bioRxiv - Cell Biology 2023Quote: ... Both membrane parts were incubated with a rabbit-anti-mouse-(1:1000; P0261; DAKO, Agilent, USA) and swine-anti-rabbit-HRP conjugated secondary antibody (1:1000 ...
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bioRxiv - Cell Biology 2023Quote: ... Both membrane parts were incubated with a rabbit-anti-mouse-(1:1000; P0261; DAKO, Agilent, USA) and swine-anti-rabbit-HRP conjugated secondary antibody (1:1000 ...