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Citations for Agilent :
1151 - 1200 of 1553 citations for Mouse KIF26B shRNA Plasmid since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... HLA-DR-and PLP-stained sections were incubated with HRP-labelled anti-mouse antibody (K5007, Dako Real EnVision detection system ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibodies used were: Goat anti-mouse IgG HRP (Cat no. P0447, Dako, 1:1500 dilution) and Goat anti-rabbit IgG HRP (Cat no ...
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bioRxiv - Cancer Biology 2023Quote: ... purified DNA from 27 mice with the Sureprint G3 mouse CGH 244K microarray kit (Agilent Technologies), as previously described11 ...
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bioRxiv - Cell Biology 2023Quote: ... we performed immunochemistry assays using mouse anti- human ki67 antibody (M7240, DAKO, 1/200 at pH9) in a series of paraffin-embedded tissue blocks of HGSOC ...
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bioRxiv - Genomics 2023Quote: ... membranes were incubated with either anti-goat or anti-mouse secondary antibodies conjugated to HRP (DAKO). Protein bands were detected with Amersham ECL™ Prime Western Blotting (RPN2236 ...
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bioRxiv - Cancer Biology 2023Quote: ... N-Universal Negative Control Anti-Rabbit or Anti-Mouse (IS600 and IS750, respectively, Dako, Glostrup, Denmark) was used ...
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bioRxiv - Immunology 2023Quote: ... bound Ab was detected using horseradish peroxidase (HRP)-conjugated rabbit anti-mouse Ig Abs (Dako, Denmark). The detection antibody was incubated for 1 hour at room temperature in a humidified atmosphere and the plates then washed five times with PBS/ 0.1% Tween 20 ...
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bioRxiv - Cell Biology 2024Quote: ... Secondary antibodies used in the study: Goat Anti-Mouse HRP (Dako, P0447, 1:5000 – 1:20,000) and Goat Anti-Rabbit HRP (Dako ...
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bioRxiv - Molecular Biology 2024Quote: ... 0.1% (v/v) NP-40] was incubated with 40 μg of rabbit anti-mouse antibody (DakoCytomation) in a vertical wheel for 90 min at 4°C ...
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bioRxiv - Genomics 2020Quote: ... a plasmid encoding the replicases of AAV serotype 2 and the capsid of AAV serotype 9) and pHelper (Agilent Technologies, lab reference pZMB0088) which provides AAV adenoviral helper functions ...
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bioRxiv - Cell Biology 2020Quote: pSecTagNC + ΔEGF_mN1 W1758A and pSecTagNC + ΔEGF_mN1 R1994A: these plasmids were generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pSecTagNC + ΔEGF_mN1 as a template and primers ...
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bioRxiv - Cell Biology 2020Quote: pcDNA3 + mNICD R1994A: this plasmid was generated by PCR-based mutagenesis using the QuikChange Lightning Multi Site-Directed mutagenesis kit (Stratagene, Santa Clara, USA) using pcDNA3 + mNICD as a template and the primer A2026VmutF.
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bioRxiv - Physiology 2020Quote: ... The QuikChange II Site-Directed Mutagenesis protocol was performed using 200 ng of plasmid DNA and PfuTurbo DNA Polymerase according to the manufacturer’s protocol (Agilent Technologies, Santa Clara, CA). Mutagenesis reactions were then digested with Dpn I (New England Biolabs ...
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bioRxiv - Cell Biology 2020Quote: ... and Nup133-mEGFP(-7) were cloned from the repair template plasmids constructed above via the QuikChange Lightning Site-Directed Mutagenesis Kit (Agilent, Santa Clara, CA). For each CRISPR cell line ...
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bioRxiv - Biophysics 2021Quote: ... were created using a 1.3mer HBV plasmid (gifted by Dr. Haitao Guo)(59) and site-directed mutagenesis kit (QuikChangeII, Agilent Technologies, Santa Clara, CA) with corresponding primers (University of Calgary DNA Synthesis Lab ...
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bioRxiv - Genomics 2020Quote: ... and electroporated in 5 separate cuvettes containing 4 µL of the plasmid and 40 µL of ElectroTen-Blue electrocompetent cells (Agilent Santa Clara, CA). After a 1 hour outgrowth in LB at 37C ...
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bioRxiv - Neuroscience 2020Quote: Adeno-associated viruses were made in HEK293T cells by co-transfecting each of the above plasmids with pAAV2 (pACG2)-RC triple mutant (Y444, 500, 730F) (Petrs-Silva et al. 2011) and pHelper (Stratagene, La Jolla, California) plasmids ...
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bioRxiv - Cell Biology 2022Quote: ... The CPE element was identified bioinformatically and was deleted from the plasmid using site-directed mutagenesis (Lightning QuikChange Mutagenesis, Agilent, Santa Clara, CA). DNA sequencing of these plasmids indicated successful deletion (Genewiz ...
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bioRxiv - Cell Biology 2022Quote: ... were introduced into the plasmid pED-FLAG-LMAN1 and pcDNA-MCFD2-Myc separately using the QuickChange site-directed mutagenesis II XL kit from Agilent (Santa Clara, CA). FVIII mutant constructs Δ807–816 (deletion of amino acids 807-816 of FVIII ...
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bioRxiv - Biochemistry 2024Quote: ... and L325A—were created using plasmids harboring the wild type GNNV-P sequence and a QuickChange Lightning site-directed mutagenesis kit (Agilent Technologies, CA, USA). Mutations were confirmed by PCR sequencing (Genomics Inc. ...
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bioRxiv - Neuroscience 2023Quote: Plasmid construct (pRK172-human-lil-synuclein) encoding WT-human-α-synuclein was expressed in BL21-CodonPlus (DE3-RIL) cells (Agilent, Cat# 230245-41). Protein expression was induced with 0.1 mM IPTG at cell density OD (600 nm ...
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bioRxiv - Microbiology 2023Quote: ... Sequence encoding the 96-120 NS2B residues was removed from recombinant pTrcHisA plasmid by PCR based site directed mutagenesis [40] using Pfu ultra II fusion HS DNA polymerase (Agilent, Santa Clara, USA) to generate recombinant plasmids expressing N-terminal hexahistidine tag fused active NS2B(H)-NS3(pro ...
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bioRxiv - Immunology 2023Quote: ... was amplified using primers listed in Table S2 and cloned into SrfI and EcoRI-linearized pCMVtag2B vector (N-terminal FLAG epitope-containing plasmid from Agilent Technologies, Cat# 211172) using Gibson Assembly (New England Biolabs ...
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bioRxiv - Genetics 2021Quote: ... performed DNA library preparation and whole-exome sequencing using Agilent SureSelect Mouse All ExonV6 kit (Agilent Technologies) following recommendations of manufacturer ...
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bioRxiv - Neuroscience 2022Quote: ... We used the following antibodies for the murine sections: anti-GFAP (1:500, Dako, raised in mouse), anti-NeuN (1:150 ...
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bioRxiv - Molecular Biology 2020Quote: Respirometry of INS1 cells and mouse islets was performed using an Agilent Seahorse XF24 respirometer (Agilent Technologies) as previously described (17) ...
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bioRxiv - Molecular Biology 2020Quote: ... membranes were incubated with rabbit anti-mouse horseradish peroxidase (HRP) conjugated antibody (P026002-2, Dako, 1:1000) as secondary antibody at RT for 1 h ...
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bioRxiv - Developmental Biology 2022Quote: ... Hybridization to 8×60K 60mer oligonucleotide spotted microarray slides (Human Mouse Genome, Agilent Technologies, design ID 074809) and subsequent washing and drying of the slides were performed following the Agilent hybridization protocol in Agilent hybridization chambers ...
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bioRxiv - Genomics 2019Quote: ... The secondary antibodies rabbit anti-mouse (#P0260) and goat anti-rabbit (#P0448) HRP were purchased from Dako Denmark ...
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bioRxiv - Cancer Biology 2019Quote: ... antigen retrieval and stained with a prediluted anti-Ki-67 antibody (Mouse, Clone MIB-1, Dako #M7240) using an automated immunostainer ...
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bioRxiv - Pathology 2019Quote: ... the sections were incubated with the biotinylated secondary antibody (goat anti-rabbit and goat anti-mouse, Dako) for 60 minutes ...
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bioRxiv - Cancer Biology 2019Quote: Negative controls were run for all antibodies with a mouse universal negative control (Dako, Carpinteria, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... HRP-conjugated polyclonal goat anti-mouse IgG and HRP-conjugated polyclonal rabbit anti-goat IgG (all Dako). Other reagents not specified below were purchased from Sigma-Aldrich (St Quentin Fallavier ...
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bioRxiv - Bioengineering 2019Quote: ... Hydrogels were incubated in a 1:100 dilution mouse anti-human CD31 primary antibody (Agilent IS61030-2) overnight at 4°C ...
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bioRxiv - Cancer Biology 2020Quote: ... Primary antibodies used were: mouse anti-human CD8 (Dako, clone C8/144B, 1:800, pH 9 retrieval), mouse anti-human CD68 (Dako ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Sections were incubated with primary antibodies as follows: Mouse anti-CD31 antibody (1:20; REF MD0823, Dako), Rabbit anti-CD90 antibody (1:100 ...
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bioRxiv - Developmental Biology 2019Quote: ... sections were incubated with Envision+ Dual Link Mouse/Rabbit HRP-linked secondary antibody (DAKO, Santa Clara, CA) for 1 hr at RT ...
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bioRxiv - Cell Biology 2019Quote: ... Secondary antibodies were conjugated to HRP and used in dilutions of 1:10000 (goat α-mouse, Dako) and 1:3000 (goat α-guinea pig ...
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bioRxiv - Immunology 2019Quote: ... The Dynabeads were then stained with F(ab’)2 FITC-Conjugated Goat Anti-Mouse Immunoglobulins (Dako, F0479) at 1:50 dilution for 1 hr ...
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bioRxiv - Microbiology 2021Quote: ... Signals were revealed using horseradish peroxidase-conjugated goat anti-mouse or swine anti-rabbit secondary antibodies (DAKO) followed by chemiluminescence using Immobilon® Western (Millipore) ...
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bioRxiv - Cancer Biology 2021Quote: ... Incubation with primary antibodies: mouse anti-human pan-Cytokeratin (1:50; clone A1/A3; Dako, Hamburg, Germany), rat anti-mouse CD31 (1:200 ...
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bioRxiv - Physiology 2021Quote: ... Génopole Toulouse Midi-Pyrénées) using Sureprint G3 Mouse GE v2 microarrays (8×60K, design 074809, Agilent Technologies), according to the manufacturer's instructions ...
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bioRxiv - Cell Biology 2019Quote: ... mouse monoclonal CDK5 antibody (invitrogen, Cat no AHZ0492) and total Tau antibody K9JA (Dako, Cat no A0024), AT8 (Thermo fisher ...
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bioRxiv - Molecular Biology 2021Quote: ... The (Cy3)-cRNA samples were hybridized to Agilent mouse 4 × 44 K-format oligonucleotide microarrays (Agilent Technologies). The slides were washed according to the manufacturer’ s instructions and scanned with an Agilent DNA microarray scanner.
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bioRxiv - Cancer Biology 2020Quote: ... and the resulting library was amplified and hybridized with a SureSelect Mouse All Exon kit library (Agilent) of RNA probes ...
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bioRxiv - Cancer Biology 2020Quote: Sections were stained with mouse or rabbit anti-human monoclonal antibodies against CD20 (Dako, L26, 1:200), CD21 (DAKO ...
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bioRxiv - Cancer Biology 2022Quote: ... primary antibodies and horseradish peroxidase-conjugated anti-rabbit/anti-mouse secondary antibodies (Dako, Santa Clara, CA, USA). Representative immunoblots are shown in supplementary Figure 3 (Fig S3).
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bioRxiv - Microbiology 2019Quote: ... Dual-IF assays were performed using mouse monoclonal antibodies directed to CD45 (clone 2B11+PD7/26; DAKO), CD3 (clone F7.2.38 ...
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bioRxiv - Molecular Biology 2021Quote: ... The secondary antibodies used were goat anti-rabbit Immunoglobulins/HRP and goat anti-mouse Immunoglobulins/HRP (Dako).
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bioRxiv - Neuroscience 2021Quote: ... Tissue sections were then incubated for 45 min with Polyclonal Anti-Mouse 1:100 (P0447, Dako-Agilent) or a BrightVision Poly-HRP-Anti Rabbit IgG ...