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Citations for Agilent :
801 - 850 of 1491 citations for Urotensin II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The enzymatic reaction product was further analyzed by high performance liquid chromatography (Agilent HPLC system (1260 Infinity II) equipped with UV-Vis DAD detector on a C-18 reverse-phase column (Phenomenex ...
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bioRxiv - Microbiology 2020Quote: ... S27 isolate-based plasmid via site-directed mutagenesis using the QuikChange II Site-Directed Mutagenesis Kit (Agilent Technologies). The following mutations were introduced ...
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bioRxiv - Plant Biology 2021Quote: Site-directed mutagenesis of PpKAI2L-H was performed using the QuickChange II XL Site Directed Mutagenesis kit (Stratagene), performed on pGEX-4T-3-PpKAI2L-H (see Supplementary Table 1 for primers) ...
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bioRxiv - Plant Biology 2020Quote: ... Two DNA fragments were generated by polymerase chain reaction (PCR) using Herculase II Fusion DNA polymerase (Agilent Technologies), primers P11 + P12 and P13 + P14 and plasmids pUCAP-pGEL3::spmCherry-pATrpC-BAR (Samalova et al. ...
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bioRxiv - Genetics 2020Quote: ... Captured libraries were amplified in 3×100 μl reactions containing 1 unit Herculase II Fusion DNA polymerase (Agilent), 1x Herculase II reaction buffer ...
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bioRxiv - Plant Biology 2021Quote: ET measurements were performed by gas chromatography (Hewlett Packard 5890 Series II) with an Al2O3 column (Agilent Technologies). Whole rosettes of 4-week-old plants grown in long-day conditions were taken ...
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bioRxiv - Microbiology 2020Quote: ... The devH upstream region was cloned between the SacI and BamHI sites of pBluescript II KS+ (Agilent Technologies), and then the devH coding region was cloned between the SalI and XhoI sites ...
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bioRxiv - Cell Biology 2022Quote: Emerin mutants were generated using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies; Santa Clara, CA) according to the manufacture’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... the stop codon was first mutagenized to glycine using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) with the STOP-to-glycine primers ...
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bioRxiv - Neuroscience 2021Quote: ... GFP-ALG-2 was obtained by performing a reverse mutagenesis (Quick change II site directed mutagenesis kit, Stratagene) on a GFP-hALG-2 Y180A (a generous gift from Masatoshi Maki ...
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bioRxiv - Immunology 2019Quote: ... R148Q mutation was generated by site-directed mutagenesis on a WT STAT2 plasmid using QuickChange II PCR (Agilent). Lentiviral particles were generated by co-transfection of STAT2 ...
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bioRxiv - Biochemistry 2020Quote: ... R256L and Y250L in AQP3 mutations were inserted using the QuikChange II kit (Agilent, Santa Clara, CA, USA), following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... Mutant libraries of CI2 WT and CI2 I57A were generated using the GeneMorph II Random mutagenesis kit (Agilent). The mutation frequency was aimed at 0-4 amino acid substitutions per gene by adjusting the initial target DNA and the number of amplification cycles ...
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bioRxiv - Neuroscience 2022Quote: ... Phospho-null mutation VASP S239A was generated using the QuikChange II Site-Directed Mutagenesis Kit (Cat.#200523, Agilent). Primers were designed using the QuikChange Primer Design Program and obtained from Integrated DNA Technologies IDT ...
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bioRxiv - Evolutionary Biology 2022Quote: Random mutagenesis of the hdp1 and hdp2 genes was performed with the GeneMorph II Random Mutagenesis Kit (Agilent) using the recommended conditions for obtaining a mean mutation rate of 1 mutation per PCR product ...
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bioRxiv - Immunology 2022Quote: ... The JH4 intronic region was amplified using a nested PCR protocol with Herculase II high-fidelity polymerase (Agilent). Primary PCR was performed for 25-cycles using V-region forward primer (5’-AGCCTGACATCTGAGGAC-3’ ...
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bioRxiv - Cell Biology 2022Quote: ... Mutations were added to wild-type H3.1-V5 and H3.3-V5 sequences using the Quikchange II XL site directed mutagenesis kit (Agilent). Lentiviruses were transfected into HEK293T cells in combination with viral packing and envelope plasmids pSPAX (Addgene 12260 ...
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bioRxiv - Bioengineering 2019Quote: ... The DNA amplification was carried out in 50 μl reactions using Herculase II Fusion DNA polymerase (Agilent Technologies) following manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... pT7/EV-A71BrCr and pCMV/hRV14 by using the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies). All mutants were verified by sequencing after mutagenesis ...
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bioRxiv - Molecular Biology 2020Quote: ... L765A) of this fragment were cloned from this vector using a QuikChange II site-directed mutagenesis kit (Agilent). The DNA construct used for proteins for crystallography was similar except it coded for PARP-1 662-1011 (again with V762A ...
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bioRxiv - Biophysics 2021Quote: ... and L207W mutations were generated by performing site-directed mutagenesis PCR using Quik Change II Mutagenesis Kit (Agilent) with the following primers:
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bioRxiv - Biophysics 2020Quote: ... Mutations were created using the QuikChange site-directed mutagenesis method with PfuUltra II Fusion HS DNA polymerase (Agilent) and a single mutagenesis primer ...
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bioRxiv - Pathology 2021Quote: ... The promoter DNA was amplified with primers containing Nhe1 and Xho1 sites using Pfu Ultra II polymerase (Agilent) to generate the 0.7 kb truncated form in the pGL3-basic vector ...
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bioRxiv - Synthetic Biology 2019Quote: ... Bruker micrOTOF-Q II instrument connected with Agilent HPLC system (Agilent G1312B SL binary; Agilent G1367C SL WP) were used to detect bikaverin and pathway intermediates ...
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bioRxiv - Synthetic Biology 2019Quote: ... Bruker micrOTOF-Q II instrument connected with Agilent HPLC system (Agilent G1312B SL binary; Agilent G1367C SL WP) were used to detect bikaverin and pathway intermediates ...
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bioRxiv - Plant Biology 2020Quote: ... Different point mutations of cysteines to serines in GRXS17 were generated using QuikChange II Directed Mutagenesis Kit (Agilent) using primers detailed in Supplemental Table 2 ...
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bioRxiv - Immunology 2021Quote: ... The gRNAs were amplified by a two-step PCR method using the Herculase II Fusion DNA Polymerase (Agilent). For the first step PCR ...
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bioRxiv - Neuroscience 2021Quote: ... and AlixΔPGY (AlixΔALG-2) cDNAs in pCI were generated by mutagenesis (Quick change II site directed mutagenesis kit, Stratagene) and Alix R757E (AlixΔendo ...
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bioRxiv - Neuroscience 2020Quote: APP-mCherry was mutated at single or double sites using the QuickChange II Site directed mutagenesis kit (Agilent) following provider-issued recommendations ...
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bioRxiv - Genetics 2021Quote: ... Real-time PCR reactions were performed with the Brilliant II SYBR® Green QPCR Master Mix (Agilent, 600828). The relative quantification in gene expression was carried out using the 2- ΔΔCt method (51) ...
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bioRxiv - Genomics 2021Quote: ... the PCR was performed in triplicate per ligation reaction using the Herculase II PCR reagents (Agilent Technologies, 600677). The parallel library preparations and PCR reactions were subsequently pooled for each reaction.
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bioRxiv - Genomics 2020Quote: The Aβ coding sequence and two flanking regions of 52bp and 72bp respectively upstream and downstream of Aβ were amplified (primers MS_01 and MS_02, Supplementary file 2) by error-prone PCR (Mutazyme II DNA polymerase, Agilent). 30 cycles of amplification and 0.01ng of initial template were used to obtain a mutagenesis rate of 16 mutations/kb ...
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bioRxiv - Biophysics 2020Quote: ... Single mutations in hCx26 and hCx30 were produced using the QuickChange II Site-Directed Mutagenesis Kit (Agilent Technologies).
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bioRxiv - Microbiology 2020Quote: ... and p-dsbS(S239A)- flag were obtained using the QuikChange II site-directed mutagenesis kit (Stratagene, catalog#: 200518). For generating p-dsbR(D51A)-flag ...
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bioRxiv - Microbiology 2020Quote: ... the primer pair dsbS(H235A)-F/R and a QuikChange II site-directed mutagenesis kit (Stratagene, catalog#:200518) were used ...
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bioRxiv - Genetics 2020Quote: ... The DNA samples were then enriched by PCR with Herculase II polymerase (Agilent Technologies, Santa Clara, CA, USA). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Mutageneses of the VqmAPhage and VqmAVc DBDs was accomplished using the GeneMorph II EZClone Domain Mutagenesis Kit (Agilent) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... Quantitative PCR analysis was performed with SYBR Premix Ex Taq II on AriaMx Real-time PCR system (Agilent). Specific primer sets for age-related genes and housekeeping gene were used ...
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bioRxiv - Immunology 2022Quote: ... Point mutations (e.g., CARD8 S297A, E274R) were generated using the QuikChange II site-direct mutagenesis kit (Agilent, 200523) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2022Quote: ... AtCLCaE203A point mutation was introduced in AtCLCa cDNA using the QuikChange II XL Site-Directed Mutagenesis Kit (Stratagene) into the Gateway vector pH2GW7.0 (Karimi et al. ...
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bioRxiv - Cell Biology 2022Quote: ... The DNA probes were labeled with [α-32P] dCTP by Prime-It II Random Primer Labeling Kit (Stratagene) as per manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... Samples of labeled and unlabeled antibody (5 – 10 μg) were run on a HPLC (Agilent 1260 Infinity II) using an Agilent AdvanceBIO SEC 300Å 2.7 mm column (PL1580-3301 ...
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bioRxiv - Cell Biology 2023Quote: ... Mutant forms of the coding regions were obtained using site-directed mutagenesis using the Quikchange II kit (Stratagene). Coding regions were transferred into Pvha-6-GFP or Pvha-6-RFP vectors by LR reaction (Invitrogen) ...
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bioRxiv - Biochemistry 2022Quote: ... These samples were run on a 1260 Infinity II Bio-Inert LC System (Agilent Technologies, Santa Clara, CA) using an AdvanceBio Glycan Mapping column 2.1 × 150 mm 2.7 micron (Agilent Technologies ...
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bioRxiv - Immunology 2022Quote: ... cDNA samples were then subjected to two rounds of PCR using Herculase II fusion DNA polymerase (Agilent Technologies).
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bioRxiv - Molecular Biology 2023Quote: ... The 5’mitoUTR was cloned into the TFAM-mScarlet/pWPXL or the 3’mitoUTR_TFAM-mScarlet_pWPXL plasmid using megaprimers following the QuikChange II XL Site-Directed Mutagenesis Kit (Agilent) large insertion protocol provided by the manufacturer ...
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bioRxiv - Neuroscience 2023Quote: ... The CLEAR mutant promoters were generated using site-directed mutagenesis (QuikChange II XL Site-Directed Mutagenesis Kit, Agilent). Renilla plasmid was transfected at 1/20 the amount of the other plasmids ...
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bioRxiv - Cell Biology 2023Quote: ... Von Zastrow lab).The DNA fragments were amplified by Pfu Ultra II Hotstart PCR master mix (Agilent Technologies) and ligated with each respective vector by NEBbuilder HiFi DNA assembly master mix (New England BioLabs).
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bioRxiv - Neuroscience 2023Quote: ... cDNA probes were labeled with 32P using the Prime-It II Random Primer Labeling Kit (Agilent Cat. #300385). The Syngap1 probe corresponds to NM_001281491 nucleotides 1361- 2002 ...
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bioRxiv - Cancer Biology 2023Quote: ... and mismatches that conferred resistance to sg5 were generated by site-directed mutagenesis (Quickchange II XL; Agilent 200521) using the oligos described in the primer list and verified by sanger sequencing ...