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Citations for Agilent :
601 - 650 of 1491 citations for Urotensin II since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... performed using QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA, USA) followed by transformation of E ...
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bioRxiv - Microbiology 2024Quote: ... Site directed mutagenesis was carried out using the QuikChange II Site-directed mutagenesis kit (Agilent).
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bioRxiv - Cell Biology 2024Quote: ... The PCR was performed using cDNA (50ng) and a Brilliant II sybr green mastermix (Agilent). Both the PCR array and qPCR were run on an Agilent AriaMx PCR machine ...
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bioRxiv - Cancer Biology 2024Quote: ... sgRNA was amplified from 50 mg gDNA using Herculase II Fusion DNA Polymerase (Agilent Technologies), column purified using Select-a-Size DNA Clean & Concentrator kit (Zymo Research) ...
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bioRxiv - Cell Biology 2019Quote: ... - Kinetex EVO (5 μm, 100 Å)) on a HPLC system (Agilent, LC 1260 Infinity II, Agilent). A two-step gradient was applied ...
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bioRxiv - Molecular Biology 2021Quote: ... Mutagenesis of all the indicated mutants was performed using the QuickChange XL II mutagenesis kit (Agilent) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The fragment was amplified with the high fidelity Pfu Ultra II Fusion HS DNA polymerase (Agilent) according to the manufacturer’s recommendations using the primers ...
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bioRxiv - Genomics 2020Quote: ... Adapter-ligated DNA was amplified for 6-8 cycles using Herc II Fusion DNA polymerase (Agilent). PCR products were purified with AMPure XP beads and subjected to Illumina paired-end sequencing (2x 75 bp).
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bioRxiv - Molecular Biology 2021Quote: ... The EcoRI sites in ZP66 were mutated to NotI using QuikChange II Sitedirected mutagenesis kit (Stratagene). Promoter specific reporters driving CFP were inserted into pUC-TALO8-NotI by digesting with NheI followed by Gibson assembly ...
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bioRxiv - Cell Biology 2019Quote: ... EGFP-MBP-BRCA2 vector using QuikChange II and QuikChange XL site-directed mutagenesis kit (Agilent Technologies), respectively (see Table EV3-EV4 for primer sequences).
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bioRxiv - Molecular Biology 2019Quote: ... MmEsco1 and MmEsco2 were introduced with the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies) according to the manufacturer’s manual and were verified by DNA sequencing.
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bioRxiv - Microbiology 2019Quote: ... a modified procedure from the Quickchange II Site Directed Mutagenesis Kit (Agilent Technologies, Santa Clara, CA) was used ...
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bioRxiv - Microbiology 2019Quote: ... Point mutations P90A and A92E were introduced using the QuikChange II site-directed mutagenesis kit (Stratagene) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2021Quote: All GFP-ITCH Full mutants were generated using QuikChange II site-directed mutagenesis kit (Agilent Technologies) according to the manufacturer’s instructions and using primers designed to introduce point mutations by changing arginine to glutamic acid (Table 1).
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bioRxiv - Molecular Biology 2021Quote: ... The mutations were introduced by site-directed PCR mutagenesis using Pfu Ultra II polymerase (Agilent Technologies), according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: Flag-TDP-43ΔNLSsiRes was generated using the QuickChange® II Site-Directed mutagenesis kit (Agilent Technologies) on pCS2-Flag-TDP-43WT (Kabashi et al. ...
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bioRxiv - Cancer Biology 2020Quote: ... Site-directed mutagenesis was conducted with the QuickChange II XL Site-Directed Mutagenesis Kit (Agilent, #200521). RIPK2m4 was generated by synonymous substitution of 648 A->T ...
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PPARγ is a tumor suppressor in basal bladder tumors offering new potential therapeutic opportunitiesbioRxiv - Cancer Biology 2019Quote: ... Point mutations were introduced into PPARγ with the QuikChange II XL Site-Directed Mutagenesis kit (Agilent), in accordance with the manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... the PH domain in HA-ORP5wt was deleted by site-directed mutagenesis (Quickchange II-XL, Stratagene) as in (Galmes et al. ...
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bioRxiv - Biochemistry 2020Quote: ... and the GEF-deficient FA mutant were made using the QuikChange II Mutagenesis Kit from Agilent. Full sequences of the parental LOV2GIV and LOV2GIVe variant are provided in Supplementary Information ...
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bioRxiv - Cell Biology 2021Quote: PCR of the insert was performed using PfuUltra II Fusion Hotstart DNA Polymerase (Agilent Technologies, #600672), following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: Site-directed mutagenesis experiments were done with the QuickChange II XL Site Directed Mutagenesis kit (Stratagene) on pGEX-4T-3-PrKAI2d3 (Supplementary Table 4) ...
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bioRxiv - Systems Biology 2020Quote: ... 0.5 μl 10 mM dNTP mix and 0.5 μl Herculase II fusion DNA polymerase (Agilent Technologies). PCR parameters were as follows ...
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bioRxiv - Plant Biology 2021Quote: ... and sugar alcohols,) were quantified using ultrahigh-performance liquid chromatography (UHPLC) 1290 Infinity II from Agilent Technologies (Santa Clara ...
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bioRxiv - Immunology 2021Quote: ... ORF9 mutant plasmids were generated using site directed mutagenesis (QuikChange II Site-Directed Mutagenesis Kit, Agilent). GST-fusion bacterial expression vectors for FLAG-ORF9 ...
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bioRxiv - Physiology 2021Quote: ... By PCR using PfuUltra II Fusion HS DNA Polymerase kit (Agilent technologies, Santa Clara, CA, USA) and the primers (Supplementary Table 1) ...
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CREB5 reprograms nuclear interactions to promote resistance to androgen receptor targeting therapiesbioRxiv - Cancer Biology 2021Quote: ... Detailed reaction conditions were followed according to a Quikchange II Site-Directed Mutagenesis Kit (Agilent, 2005230). The mutant clones were each sequenced to confirm their identities before subsequent use in recombination reactions ...
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bioRxiv - Cell Biology 2021Quote: ... All point mutations were introduced with the QuikChange II XL site-directed mutagenesis kit (Agilent Technologies). All plasmids were sequenced to verify desired mutations and absence of unintended mutations.
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bioRxiv - Cell Biology 2021Quote: ... The E-box mutant was constructed with QuikChange II XL Site-Directed Mutagenesis Kit (Agilent Technologies) using primers 5’-cgcgggttcccaccttgtggccagaagatcttctgggcagaactactcgtttggc-3’ and 5’-gccaaacgagtagttctgcccagaagatcttctggccacaaggtgggaacccgcg-3’ ...
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bioRxiv - Cell Biology 2020Quote: ... The CEL-TRUNC construct was generated by QuikChange II XL site-directed mutagenesis kit (Agilent Technologies) using pcDNA3/CEL-WT 14R as template (20).
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Large-scale conformational changes of FhaC provide insights into the two-partner secretion mechanismbioRxiv - Biophysics 2022Quote: ... Point mutations in fhaC were generated using the QuikChange II XL Kit (Agilent, Les Ulis, France) on pFc3 (Guedin et al. ...
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bioRxiv - Neuroscience 2022Quote: ... we used the Quick Change II XL site-directed mutagenesis kit (Agilent Technologies, cat. no. 200522) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The site-directed mutant NqrB-Glu157Ala was obtained using the QuickChange II XL mutagenesis kit (Agilent), as reported before (30) ...
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bioRxiv - Molecular Biology 2022Quote: ... Longer inserts flanked by longer homology arms were cloned into pBluescript II KS-plasmid (Agilent Technologies). All TALEN and Cas9 target sequences and donor sequences for homologous recombination are listed in Table S1 ...
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bioRxiv - Immunology 2022Quote: ... were introduced into pTT3-gH-His using the QuickChange XL II Kit (Agilent, Cat. number #200521) according to manufacturer’s instructions.
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bioRxiv - Physiology 2022Quote: ... PIMT and TRAF6 mutants were made using the QuikChange II Site-Directed Mutagenesis Kit (Agilent, #200523). All original constructs in this study were verified by DNA sequencing.
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bioRxiv - Cancer Biology 2022Quote: Agilent 1290 Infinity II ultra-performance liquid chromatography (UPLC) system (Agilent Technologies, Palo Alto, CA, USA) coupled online to the Dual AJS electrospray ionization (ESI ...
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bioRxiv - Developmental Biology 2022Quote: ... pax8CNS1mut-lucHP123) were obtained with the QuikChange® II XL Site-Directed Mutagenesis Kit (Agilent Technologies) using primers listed in Supplementary Table 4 ...
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bioRxiv - Cell Biology 2022Quote: ... and EMC6-N22A were constructed using QuikChange II site-directed mutagenesis Kit (Agilent Genomics, catalog #: 200523). All cDNA sequences were confirmed by DNA sequencing.
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bioRxiv - Genomics 2020Quote: ... Library quality control and quantification were performed with a Bioanalyzer Chip DNA 1000 series II (Agilent), and sequenced in a HiSeq2000 platform (Illumina ...
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bioRxiv - Cancer Biology 2020Quote: ... qRT-PCR was performed with Brilliant II Ultra-Fast SYBR® Green QPCR Master Mix (Agilent) using a Bio-Rad CFX96 Real-Time Detection System ...
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bioRxiv - Biochemistry 2021Quote: Antibody gene mutations were introduced by QuikChange II site directed mutagenesis kit (Agilent, Cat. No. 200524)
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bioRxiv - Biochemistry 2020Quote: ... The PcATP2-D596N mutant was obtained using the QuikChange II XL site-directed mutagenesis kit (Agilent). The nine Cdc50 subunits sequences were cloned in the pYeDP60 vector between the EcoRI and BamHI restriction sites ...
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bioRxiv - Microbiology 2022Quote: ... primers were designed according to the protocol of the QuickChange II Site-directed mutagenesis kit (Agilent) and as listed in table S2 ...
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bioRxiv - Biochemistry 2022Quote: ... Site-directed mutagenesis was carried out using QuikChange II XL site-directed mutagenesis kit (Agilent Technologies) according to the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2019Quote: ... The PCR product was digested with HindIII and XhoI and cloned in pBluescript II SK (Stratagene). The fragment containing hofM ...
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bioRxiv - Biochemistry 2019Quote: ... All the mutations were introduced with the QuikChange II XL site-directed mutagenesis kit (Stratagene, 200522) and confirmed by DNA sequencing ...
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bioRxiv - Cancer Biology 2019Quote: ... resuspended in 20 µL of a Herculase II-containing PCR mix (catalog number 600679, Agilent Technologies), and ran for 20 PCR cycles followed by a second 20-cycle PCR using 2 µL of the initial PCR products to add barcodes and P5/P7 Illumina sequencing adapters ...
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bioRxiv - Animal Behavior and Cognition 2020Quote: ... High fidelity amplification was achieved using Herculase II Fusion DNA Polymerase (Agilent, Santa Clara, California, USA) and a touchdown thermal profile ...
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bioRxiv - Plant Biology 2021Quote: ... site-directed mutagenesis was carried out with the Quick Change II Site-directed mutagenesis Kit (Agilent) according to the manufacturer’s instructions ...