Labshake search
Citations for Leica :
1951 - 2000 of 2407 citations for 7 Chloro 1 3 dihydro imidazo 4 5 c pyridin 2 one since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... leaving around 2 mm of the root outside the needle which was submerged in a tissue-freezing medium (Jung, Leica) and quickly frozen in liquid nitrogen (Vogel-Mikuš et al. ...
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bioRxiv - Neuroscience 2023Quote: ... The caudal corpus callosa alongside adjacent tissue were punched with a 2 mm diameter punching tool and embedded in Epon (EM TP, Leica) prior to conventional embedding steps ...
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bioRxiv - Microbiology 2023Quote: ... The emitted fluorescence resulting from Fluo-8 or Rhod-2 AM staining was observed using a fluorescence microscope (DM6000B,Leica) with a 488-nm or 543-nm filter ...
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The transcriptomic landscape of monosomy X (45,X) during early human fetal and placental developmentbioRxiv - Genetics 2024Quote: ... IHC for CSF2RA (Colony Stimulating Factor 2 Receptor Subunit Alpha) was undertaken on 3µm sections using a Leica Bond-max automated platform (Leica Biosystems). In brief ...
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bioRxiv - Systems Biology 2024Quote: ... Dried smears were incubated in 70% ethanol for 2 hours and hematoxylin/eosin (H&E) stained using an automatic Leica Multistainer and Coverslipper (Leica ST5020+CV5030 ...
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bioRxiv - Neuroscience 2020Quote: ... the rats were sacrificed via transcardiac perfusion with 4% paraformaldehyde (PFA) using the Perfusion Two™ Automated Pressure Perfusion System (Leica Biosystems, Buffalo Grove, IL) (n = 12 ...
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bioRxiv - Cancer Biology 2020Quote: ... was performed on 4-μm FFPE sections arranged on the above mentioned TMA using commercial equipment (Leica Bond RXm System; Leica Microsystems Ltd, Milton Keynes, UK) and a detection kit (Bond Polymer Refine Red Detection Kit ...
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bioRxiv - Neuroscience 2021Quote: The whole-mount samples were embedded in gelatin/albumin with 4% of Glutaraldehyde and sectioned at 20 μm thickness on a vibratome (Leica, VT1000 S vibrating blade microtome). The sections were mounted in Fluoromount Aqueous Mounting Medium (Sigma ...
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bioRxiv - Neuroscience 2023Quote: ... Brains were then fixed in 4% PFA overnight before sectioning 50 µm coronal slices using a Leica VT1000S vibratome (Leica Biosystems, Deer Park, IL, USA).
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bioRxiv - Neuroscience 2023Quote: ... Post-fixed brains (PFA 4%) were coronally sectioned at either 30 μm (IHC) with a cryostat (Leica CM 1950, Leica Biosystems, Deer Park, IL, USA). Mice left tibia was dissected and placed in 4% PFA then follow 2.10 Bone histology analysis method.
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bioRxiv - Neuroscience 2024Quote: ... Brains were post-fixed with PFA 4% overnight and 50 µm thick coronal sections were cut with a VT 1000S vibratome (Leica Microsystems Inc., Buffalo Grove, IL). Free-floating vibratome sections were processed as follows ...
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bioRxiv - Microbiology 2019Quote: ... at pH 2.5 to quantify mucous cells in the gills per inter-lamellar unit (ILU) under a bright field light microscope (Leica DM1000, Hamburg, Germany) (29 ...
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bioRxiv - Immunology 2022Quote: ... was performed on sections (5 mm thick) of formalin-fixed lung mounted onto charged glass slides using the Leica Bond RX automated system (Leica Biosystems, Richmond, IL). Heat-induced epitope retrieval was conducted by heating slides to 95°C for 15 minutes in EDTA-based ER2 buffer (Leica Biosystems) ...
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bioRxiv - Neuroscience 2020Quote: ... and 11 glucose saturated with 95% O2–5% CO2 and horizontal brain slices (150 μm thick) containing the VTA were prepared using a Vibratome (Leica Instruments, Nussloch, Germany). Slices were and allowed to recover at 35°C for at least 1 hr before recordings were initiated ...
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bioRxiv - Microbiology 2022Quote: ... and approximately 3.5 µL of purified virus particles were applied to freshly glow-discharged (10 mA for 30 sec using a Leica Ace600 glow-discharger) Quantifoil R2/1 300-mesh holey carbon-coated copper grids ...
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bioRxiv - Bioengineering 2022Quote: ... 3.033 μm and z: 5 μm) of the intervention hemisphere were acquired using a confocal laser scanning microscope SP8 (Leica Microsystems, Wetzlar, Germany) with a 10x objective ...
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bioRxiv - Plant Biology 2021Quote: ... The root segments were embedded in agarose 5% (w/v) and cut into 25-30 μm radial sections with a VT1200S vibratome (Leica Microsystems, Nanterre, France). Radial sections were sampled from 15 ectomycorrhizal rootlets (at 200 µm from the root apex ...
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bioRxiv - Microbiology 2022Quote: ... tremulum culture was stained with Auramine O and examined by using the Leica TCS SP 5 Confocal Laser Scanning Microscope (Leica Microsystems, Wetzlar, Germany) in the Laboratory of Bioimaging facility NCCS ...
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bioRxiv - Cancer Biology 2019Quote: ... Sections were examined with an Olympus BX43 light microscope and microphotographs taken using a 5 Megapixel HD Microscope Camera (Leica MC170 HD, Germany).
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bioRxiv - Cancer Biology 2021Quote: Sections with a thickness of 5 µm of lung adenocarcinoma and matched adjacent non-tumor lung tissue were mounted on MembraneSlides (Leica Microsystems, Wetzlar, Germany) and stained with hematoxylin and eosin ...
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Serotonin, Etonogestrel and breathing activity in murine Congenital Central Hypoventilation SyndromebioRxiv - Physiology 2022Quote: ... and c-FOS/5-HT immunolabeled cells were visually counted by an investigator blinded to samples using a light microscope (Leica DM2000; Leica Microsystems, Heidelberg, Germany) at high magnification (x200 or x400 depending on the immunolabeled density of cells) ...
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bioRxiv - Zoology 2022Quote: ... and sectioned serially into 5 μm transverse or coronal tissue sections using the Leica X Multicut semi-automated rotary microtome (Leica Biosystems, Wetzlar, Germany). Additional samples from juvenile T ...
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bioRxiv - Plant Biology 2022Quote: Sections of 5 or 10 μm were prepared on a Leica JUNG RM2055 microtome using a microtome knife (Leica Disposable blades TC-65). The sections were imaged without staining or after staining with Safranin O (Sigma-Aldrich ...
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bioRxiv - Pathology 2023Quote: ... The tissue samples were decalcified and embedded in paraffin before the preparation of coronal sections of 5-μm thickness around the ligation site using a microtome (RM2245, Leica Microsystems, Wetzlar, Germany). Tissue sections were then subjected to TRAP staining using an acid phosphatase leukocyte kit (Sigma-Aldrich ...
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bioRxiv - Physiology 2024Quote: ... H-1000) and visualized using a Leica Stellaris 5 Confocal Microscope equipped with a 40x oil-immersion objective (Leica Microsystems Inc, Illinois, USA). Fluorescence intensity profiles across sarcomeres were plotted offline using the FIJI software.
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bioRxiv - Evolutionary Biology 2023Quote: Rehydrated brains were embedded in agarose gel (5% UltraPure agarose power in deionised water) and sectioned using a vibrating blade microtome with an 80 µm interval (Leica VT1200, Wetzlar, Germany). Sections were then washed six times in PBS-TX (0.5% Triton X-100 detergent in phosphate-buffered saline) ...
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bioRxiv - Cancer Biology 2021Quote: ... CD4 (1:100, HIER 1, mouse monoclonal, clone 4B12, Leica, Germany; CD4-368-L-CE-H), CD8 (1:50 ...
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bioRxiv - Molecular Biology 2019Quote: ... Images were acquired using Leica DFC 3000G (Bin 1×1, Gamma1) camera and processed by Leica LAS X imaging software.
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bioRxiv - Cancer Biology 2021Quote: ... and p53 (D07, 1:50, Leica) were applied with negative controls as previously described2.
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Hypusinated eIF5A is expressed in pancreas and spleen of individuals with type 1 and type 2 diabetesbioRxiv - Cell Biology 2019Quote: ... mouse anti-CD4 (Leica; 1:500). Secondary antibodies including Alexa-488 ...
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bioRxiv - Genomics 2021Quote: ... mouse Ki67 (1:1000, Leica, ACK02), rabbit Lyve1 (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... ER Solution 1 (AR9961, Leica Biosystems) was used ...
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bioRxiv - Cell Biology 2020Quote: ... The loosely packed cells in BSA were high-pressure frozen in a Leica Empact 2 high-pressure freezer (Leica, Vienna, Au), and submitted to a quick freeze-substitution protocol that preserves fluorescence (35 ...
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bioRxiv - Developmental Biology 2019Quote: ... mounted on fixed 45° pretilt SEM stubs and coated with a 2 nm layer of platinum using a Leica Microsystems EM ACE600 sputter coater (Leica Microsystems) to enhance electrical conductivity ...
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bioRxiv - Physiology 2021Quote: ... Slides were prepared using the frozen slide delay prior to pre-treatments using Epitope Retrieval Solution 2 (Cat No. AR9640, Leica Biosystems) at 95°C for 5 min ...
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bioRxiv - Biophysics 2021Quote: ... Then 3 μL of concentrated hTg were dispensed to the hydrophilic surface of the grid prior to single side blotting for 2 s and plunge freezing in liquid ethane using a Leica EM GP2 plunger (Leica Microsystems) operating at 20 °C and 80% relative humidity.
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bioRxiv - Evolutionary Biology 2022Quote: ... The fixed samples were embedded in Technovit 8100 (Kulzer, Germany) and processed into 2 μm tissue sections using a rotary microtome RM2255 (Leica, Germany). The AlexaFluor555-labeled oligonucleotide probes Eco934 (5’-CAT GCT CCA CCG CTT GTG-3’ ...
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bioRxiv - Physiology 2021Quote: ... Images were acquired at a rate of 50 Hz with a wide-field fluorescence microscope (DMIRE-2, Leica Microsystems, Wetzlar, Germany) as previously described [24].
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bioRxiv - Immunology 2020Quote: ... The uptake of the fluorescently labeled SVV in IBRS-2 and 293T cells was visualized with a fluorescence microscope (Leica, Germany).
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bioRxiv - Neuroscience 2019Quote: ... The brains were then removed and postfixed in 4 °C overnight and immersed in 30% sucrose solution for 2 days before being sectioned at 50 μm thicknesses on a cryostat (CM3050 S; Leica Biosystems). Imaging was performed with a scanning microscope (BX61VS ...
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bioRxiv - Cell Biology 2019Quote: ... After immobilization with 2 mM levamisole aggregation was scored using a fluorescent stereo microscope (Leica M165 FC, Plan Apo 2.0× objective). For PAB-1 ...
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bioRxiv - Developmental Biology 2020Quote: ... Wild-type 1 dpf larvae were positioned on top of a 2% agarose plate and imaged using a Leica M165FC dissection scope (Leica Microsystems) with a SPOT RT3 camera (SPOT Imaging) ...
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bioRxiv - Neuroscience 2020Quote: ... Fish were imaged for 6 min taking 2 μm thick stacks through the whole brain using a confocal microscope (Leica SP8). Each fish was imaged every 20 mins for 8 hours.
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bioRxiv - Plant Biology 2020Quote: ... mesophyll cells of 2-week-old transgenic Arabidopsis plants expressing Raf36-GFP were observed with a confocal microscope SP8X (Leica Microsystems) with the time-gating method (Kodama ...
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bioRxiv - Neuroscience 2020Quote: ... Coronal slices (300 µm thick) containing visual cortex were prepared using a vibrating blade microtome (Leica VT1200S or Campden 7000smz-2). Slices were immediately transferred to a submerged holding chamber with regular ACSF containing (in mM) ...
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bioRxiv - Immunology 2021Quote: ... Ultra-thin (50 nm to 60 nm) sections were sectioned with a diamond knife (Jumdi, 2 mm) on an ultramicrotome (Leica Ultracut), contrasted with 2% uranyl acetate (Polyscience ...
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bioRxiv - Cancer Biology 2022Quote: ... Sections were first deparaffinized and then underwent antigen retrieval by incubating with EDTA-based epitope retrieval solution (BOND Epitope Retrieval Solution 2, Cas#AR9640, Leica BioSystems) for 20 minutes ...
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bioRxiv - Developmental Biology 2022Quote: ... Z-stacks of the cuticular side of the VL3 or VL4 muscles from abdominal segment 2 were acquired using a SP5 laser-scanning microscope (Leica Microsystems) with either a HC PL APO CS2 20x/0.75 (late third instars ...
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bioRxiv - Neuroscience 2023Quote: ... The brain was let chill for ∼2 min before slicing in ice-cold sucrose-ACSF using a vibratome (Leica VT1000S, Leica, Germany). Brain slices were immediately transferred to a submerged holding chamber containing room-temperature holding ACSF composed of (mM) ...
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bioRxiv - Cell Biology 2022Quote: ... 40 s) Quantifoil Cu 300 R2/2 grid and plunge frozen in liquid ethane with a GP2 (Leica Microsystems, Wetzlar, Germany) blotted from behind ...