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Citations for Polysciences :
1251 - 1300 of 3018 citations since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... cells were transfected with lentiviral packaging plasmids (pSPAX.2 and pMD2.G) and lentivirus vector Per2::dLuc using PEI Max (Polysciences). At 48 hours post-transfection ...
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bioRxiv - Cell Biology 2023Quote: ... Polyethyenimine (PEI) (Polysciences, 23966-1) was used as a transfection reagent ...
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bioRxiv - Cancer Biology 2023Quote: ... Plat-E cells were seeded into 6-well plates and transfected with pMXs-Venus-IRES-PuromycinR and pMXs-HOXB7-IRES-PuromycinR plasmid DNAs using 6 μg polyethylenimine (PEI; Polysciences, Inc., PA, USA). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... Transfection was performed using Polyethylenimine (PEI) (Polysciences, 23966) according to the manufacturer’s protocol ...
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bioRxiv - Biophysics 2023Quote: ... Negative staining was performed using 1% uranyl acetate (98% uranyl acetate; ACS Reagent, Polysciences Inc. Warrington, PA, USA) solution for 20 s ...
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bioRxiv - Cell Biology 2023Quote: ... cells were transiently transfected with plasmid DNA using PEI (Polysciences, 24765) according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... the infiltration with Lowicryl HM20 (Polysciences) was performed using increasing resin concentration in steps of 6h each ...
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bioRxiv - Cancer Biology 2023Quote: ... lentiCRISPR v2 plasmids [81] were transfected using polyethylenimine (Polysciences) into 293T cells along with psPAX and pMD2.G packaging plasmids (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... The cells were transfected with 121.6 μL of 1 mg/mL of linear Polyethylenimine (PEI; MW 25,000; Polysciences) and 30.4 μg of plasmid DNA diluted in Opti-MEM (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... stained with DAPI and mounted on glass slides with Aqua PolyMount (Polysciences, Inc.). Images were acquired with a confocal microscope LSM700 (Zeiss ...
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bioRxiv - Cell Biology 2023Quote: ... followed by 3 gentle washes with PBS and finally samples were mounted on glass slides with Aqua PolyMount (Polysciences, Inc.).
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bioRxiv - Cell Biology 2023Quote: ... were diluted 1:5 in 10% polyvinyl alcohol (Mowiol 4-88, 17951, Polysciences Inc.) in Milli-Q water and spun-coat onto plasma-cleaned coverslips (#1.5H ...
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bioRxiv - Biophysics 2023Quote: ... using polyethyleneimine (PEI) (Polysciences, Cat. no: 19850) at a ratio of 3:1 (PEI:DNA 3:1) ...
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bioRxiv - Biophysics 2023Quote: ... 5000x diluted stock solution of polystyrene beads (Polysciences, USA) in ethanol (Carl Roth ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were rinsed in cold tap water for two minutes and then stained in Beibrich Scarlett-Acid Fuchsin Solution (Polysciences) for 15 seconds ...
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bioRxiv - Cell Biology 2023Quote: Room temperature cryosections were fixed in Bouin’s Fixative (Polysciences) for 20 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... 1% Acetic Acid (Polysciences) was pipetted directly onto the sections for one minute ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were stained in Aniline Blue (Polysciences) for 30 seconds and rinsed in DI three times for 30 seconds ...
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bioRxiv - Cell Biology 2023Quote: ... Phosphotungstic/Phosphomolybdic acid (Polysciences) was added directly to the tissue sections ...
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bioRxiv - Cell Biology 2023Quote: ... Slides were rinsed in cold tap water for five minutes and then stained in Weigert’s Iron Hematoxylin working solution (Polysciences) for five seconds ...
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bioRxiv - Cell Biology 2023Quote: ... for HeLa cells or Polyethylenimine (PEI) Max (Polysciences, Warrington, PA, USA) for 293T cells ...
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bioRxiv - Cell Biology 2023Quote: ... 25,000 Da (1mg/ml; Polysciences, Inc.). The transfection ratio was 1 μg plasmid:3 μL PEI ...
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bioRxiv - Cell Biology 2023Quote: ... Beads (Fluoresbrite, 2 µm diameter, Polysciences, Warrington, PA) were diluted 1:500 in phosphate buffered saline ...
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bioRxiv - Cancer Biology 2023Quote: ... All transfections were carried out using polyethyleneimine (PEI Max 40K #24765, Polysciences, Warrington, PA) reagent at a 4:2:3 ratio of sgRNA/overexpression-construct ...
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bioRxiv - Immunology 2023Quote: ... 1.2 μg of DNA was mixed with 2.4 μL of 1 mg/mL polyethylenimine (linear PEI, MW 25,000, Polysciences) in one tenth of the final culture volume of medium ...
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bioRxiv - Genomics 2023Quote: ... Polyethylenimine linear MW 25000 transfection grade (PEI 25K) (cat. # 239661) from Polysciences Inc. ...
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bioRxiv - Immunology 2023Quote: 50,000 of thymocytes from Satb1+/Venus mice were resuspended in 0.1mL RPMI-1640 medium (10% FBS) and were mounted on a poly-L-lysine-coated glass slide (Polysciences, Inc., REF: 26414) and incubated at 37 °C for 2 hours ...
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bioRxiv - Genetics 2023Quote: ... flies were dry starved for 2h and then fed Fluoresbrite microspheres (Polysciences, 17149 (0.05μm, green) or 195075 (0.5μm ...
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bioRxiv - Genetics 2023Quote: ... or recombined forms of eG-SCON-FRT-FP was mixed with 8 μl of polyethyleneimine (1 mg/ml; Polysciences, 23966). These solutions were then incubated at room temperature for at least 15 minutes before being added dropwise to the cells ...
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bioRxiv - Genetics 2023Quote: ... coverslips were washed twice with PBS supplemented with 0.1 % Triton-X-100 and once with PBS and mounted with aqua-poly/mount (Polysciences Inc). Cells were imaged using a Zeiss LSM700 microscope equipped with a Plan-Apochromat 40x/1.3 Oli DIC M27 immersion lens (Carl Zeiss Microimaging Inc.) ...
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bioRxiv - Cell Biology 2023Quote: HEK293T cells (ATCC, Manassas, USA) were transfected with pNL-EGFP/pVSV using polyethylenimine (PEI; Polysciences, Germany) (PEI/DNA 6/1 ratio ...
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bioRxiv - Cell Biology 2023Quote: ... and pMD2.G using PEI Max 40000 (PolySciences, USA). The supernatant containing lentiviral particles was harvested and mixed with forty percent PEG 8000 (Sigma Aldrich ...
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bioRxiv - Developmental Biology 2023Quote: ... Fluorescent 2 μm beads (Polysciences Fluoresbrite® YG Microspheres 2.00μm 18338-5 ...
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bioRxiv - Developmental Biology 2023Quote: ... pcDNA3.0-3XFLAG-Cry2 plasmids and PEI max 40k (Polysciences Inc CN: 24765-1). 24 hours after transfection ...
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bioRxiv - Immunology 2023Quote: ... or Myc-tagged constructs by using polyethylenimine (PEI, Polysciences, 23966). After 24 hr later ...
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bioRxiv - Immunology 2023Quote: 1,200 synchronized L1 worms were mixed with 6 μl fluorescent beads (Fluoresbrite Polychromatic Red Microspheres, Polysciences Inc.), 25 μl 10X concentrated OP50-1 E ...
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bioRxiv - Developmental Biology 2023Quote: ... and the embryos were embedded laterally in Aqua-Poly/Mount (Polysciences).
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bioRxiv - Cell Biology 2023Quote: ... and 144 μl of 1 mg/ml polyethylenimine (PEI) (Polysciences) were mixed in 9 ml OptiMEM (Thermo) ...
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bioRxiv - Cell Biology 2023Quote: ... the specimens were mounted in Aqua-Poly/Mount (Polysciences), and imaged using an Olympus IX71 inverted fluorescent microscope.
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bioRxiv - Cell Biology 2023Quote: ... plasmids were generally transfected at 50-60% of confluency using PEI (polyethyleneimine, Polysciences Inc #23966-2)/ Lipofectamine LTX (Invitrogen #15338100 ...
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bioRxiv - Cell Biology 2023Quote: ... 400 ug of the MAXI-prep DNA that was pre-diluted in 13 ml of Opti-MEM® I Reduced Serum Medium (Thermo, 31985-062) and 800 ug Polyethylenimine (PEI 25K, Polysciences CatNo 23966-1) likewise pre-diluted in 13 ml of Opti-MEM media ...
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bioRxiv - Molecular Biology 2023Quote: ... 30 µl transfection reagent PEI (1 µg/µl, Polysciences) was added and incubated for 30 min at RT and added to the 10 cm dish with HEK293T cells ...
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bioRxiv - Microbiology 2023Quote: ... Slides were then transferred directly into aniline blue (Polysciences, 25088E) for 5 min and differentiated in 1% acetic acid (Polysciences ...
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bioRxiv - Microbiology 2023Quote: ... The slides were then serially stained with Weigert Hematoxylin (Polysciences, 25088B1&B2) for 10 min ...
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bioRxiv - Biochemistry 2023Quote: ... cells were transiently transfected with plasmid DNA containing GFP tagged protein of interest using PEI Max transfection reagent (24765; Polysciences). Briefly ...
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bioRxiv - Biochemistry 2023Quote: ... and DAPI in PBS+ for 1.5 hr at room temperature before mounting with Aqua-Poly/Mount (Polysciences, USA). Cells were imaged using a Zeiss Axio Imager 2 fluorescent microscope and cells with more than 5 RAD51 foci were manually counted.
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bioRxiv - Bioengineering 2023Quote: ... Plasmid DNA and polyethyleneimine (PEI, Polysciences) were independently diluted to 0.05 and 0.1 mg/mL ...
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bioRxiv - Cell Biology 2023Quote: ... and 4.5 µm (Polysciences) were washed twice with 100 mM 2-(N-morpholino ...
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bioRxiv - Cancer Biology 2023Quote: ... HEK293T cells were transfected using Polyethylenimine (PEI; Polysciences), and Ishikawa and AN3CA cells were transfected using Lipofectamine2000TM (Thermofisher ...
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bioRxiv - Biochemistry 2023Quote: ... polyethyleneimine from Polysciences, and the trypsin-EDTA solution from Gibco ...