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Citations for VWR :
1451 - 1500 of 1558 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... Single cells were sorted into 200 μL fresh culture medium in one well of a 96-well flat bottom plate (VWR Catalog #29442-054).
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bioRxiv - Biophysics 2021Quote: ... the fluidic sample cells were constructed using two pieces of double-sided tape sandwiched between a microscope slide and glass coverslip (VWR 22×30 mm). Each microscope slide had a hole on each of two ends ...
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bioRxiv - Cell Biology 2020Quote: ... The cultivation media used for each cell line was recommended by the supplier with an addition of 10% Fetal Bovine Serum (FBS, VWR, Radnor, PA, USA). For immunostaining ...
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bioRxiv - Microbiology 2021Quote: ... Vero cells were seeded at 60% confluence in 24-well plates equipped with 13 mm round glass coverslips (VWR, cat n. 631-0150). Cells were washed once with PBS before incubation with BetaCoV/Australia/VIC01/2020 diluted in PBS at an MOI=5 ...
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bioRxiv - Immunology 2020Quote: ... Fused cells were then seeded on 96 well flat bottom plates onto mouse peritoneal cells as feeder layer in hypoxanthine-aminopterine-thymidine (VWR, Radnor, Pennsylvania, USA) medium for the selection process.
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bioRxiv - Genetics 2019Quote: We performed cell culture using the conditions described above in 12-well plates containing sterile 18-mm glass cover slips (VWR, Cat#48380-046). We fixed cells for 10 minutes in 4% paraformaldehyde (Sigma-Aldrich ...
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bioRxiv - Biochemistry 2022Quote: ... The cell suspensions were filtered through a 35 μm nylon filter cap on 12 × 75 mm round bottom tubes (VWR/Falcon 21008-948). Flow cytometric measurements were performed using a BD LSR Fortessa ...
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bioRxiv - Genomics 2022Quote: ... HeLa S3 or HeLa S3 + TOP2A- Venus cell linescells were either grown or spun onto 22×22mm No 1.5 coverslips in 6-well plates (VWR Cat No 48366-277). (cell cycle analysis experiments ...
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bioRxiv - Immunology 2022Quote: ... PBMCs (4×105 cells/well) were laid in sterile round bottom 96-well tissue culture treated plates (VWR, The Netherlands, cat #734-2184) in Dutch modified RPMI 1640 medium containing 50 µg/mL Gentamicine ...
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bioRxiv - Microbiology 2023Quote: ... Adjusted cultures were then mixed in a 2.5:97.5 ratio of fluorescent:non-fluorescent cells and nine ml of this mixture was added to 13×100mm culture tubes (VWR, Cat no. 10545-936) and grown in 25°C incubator for three days ...
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bioRxiv - Bioengineering 2024Quote: ... PCLS were then placed on Gelfoam® for long-term culture and monitored using a WST-8 cell counting kit (VWR #89155-898) according to the kit’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: 5×106 MM.1Sgfp+/luc+ cells were treated with DMSO or 1.00 μM TriC for 24 h in T-25 flasks (Avantor/VWR; Cat. no. 10861-568). Cells were then harvested ...
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bioRxiv - Evolutionary Biology 2021Quote: ... All evolution was conducted at 30°C in 128μL of YPD (a rich laboratory media; 1% Bacto yeast extract (VWR #90000– 722), 2% Bacto peptone (VWR #90000–368) ...
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bioRxiv - Immunology 2020Quote: RNA was extracted using a modified phenol-chloroform protocol (Chomczynski and Sacchi, 2006) with 1-Bromo-3-chloropropane and Isopropanol (Sigma and VWR, respectively). Total RNA was quantified and assessed for quality and integrity by Bioanalyser (RNA Pico 6000 Chip ...
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bioRxiv - Molecular Biology 2021Quote: We prepared Aβ1-42 oligomers (oAβ) and fibrils (fAβ) following Evans (2006) and Ryan et al (2010) using siliconized polypropylene tubes (VWR #4165SL). We used Aβ1-42 to test the effects of the hybrid protein because it is more predominant in AD plaques (Nirmalraj et al. ...
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bioRxiv - Bioengineering 2021Quote: ... Stained tissue sections were washed in 0.1% Triton-X/PBS at RT for 1 hour and mounted on a poly-L-lysine coated coverglass (VWR, 48393-241) for imaging (Fig ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then normalized to 1 ng μL-1 or 2 ng μL-1 for genomic DNA extracted from fecal and cecal samples by diluting in molecular grade water (VWR International) using a Tecan Evo Liquid Handling Robot ...
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bioRxiv - Neuroscience 2020Quote: ... Individual CNSs were sonified with five 0.3 s pulses at 1 Hz using a Branson Sonifier 450 (VWR Scientific, Winchester, PA) in Laemmli buffer (Bio-Rad ...
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bioRxiv - Microbiology 2020Quote: ... Both filtered and unfiltered water samples were vortexed and diluted 1:5.75 into 2% (vol/wt) trace-grade nitric acid (VWR, Radnor, PA) in acid-washed polypropylene tubes and stored overnight at ambient temperature ...
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bioRxiv - Genetics 2022Quote: ... The slide was then transferred to 37°C for 1 min before being immersed and fixated in methanol (VWR EU, 20847.307) at -20°C for 30 min ...
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bioRxiv - Microbiology 2020Quote: ... The 12 lineages were evolved in 1 mL of antibiotic-free LB medium using 2 mL-deep-96-well plates in checkered pattern (VWR International) and incubated at 37°C with 700 rpm constant shaking (Microplate Shaker TiMix 5 ...
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bioRxiv - Plant Biology 2021Quote: ... and the seeds were removed with forceps and put in a drop of clearing solution (1 vol glycerol / 7 vol chloral hydrate liquid solution, VWR Chemicals) between a slide and a coverslip ...
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bioRxiv - Microbiology 2021Quote: Polar metabolites were first extracted from the frozen SHIME-samples by means of ultra pure water (0,055 μS cm-1) obtained via a purified water system (VWR International, Merck). For this purpose ...
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bioRxiv - Bioengineering 2022Quote: ... containing 20% FBS (Wisent, 80450, lot 115714), 1% Penicillin-Streptomycin solution (Wisent, 450-201-EL) and 2,5 ng/mL bFGF (VWR, 10821-962) in a 37°C ...
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bioRxiv - Immunology 2020Quote: ... the monocytes were harvested and the pellet resuspended in differentiation media (RPMI-1640 supplemented with 1% FBS H.I., Penicillin/Streptomycin (P/S, VWR, Radnor, PA) and 100 ng/mL phorbol 12-myristate 13-acetate (PMA ...
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bioRxiv - Microbiology 2019Quote: ... Growth in the phylloplane was assayed by re-isolating Protomyces from inoculated leaves by placing leaves in 2 ml Eppendorf tubes with 1 ml 0.025% Silwet-L77 in water and shaking (800 rpm; VWR microplate shaker) for 1 hour ...
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bioRxiv - Genomics 2020Quote: Single cell suspensions in PBS with 2% FBS were stained by incubating for 15 minutes at room temperature protected from light with anti-human PTPRC (CD45) monoclonal antibody conjugated to FITC (1:200 dilution, VWR #ABNOMAB12230), anti-human EPCAM antibody conjugated to PE (1:50 dilution ...
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bioRxiv - Immunology 2021Quote: ... Antibodies were diluted in PBS with 2% FBS and samples were stored in PBS containing 1% formaldehyde (VWR Life Science AMRESCO). For intracellular staining ...
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bioRxiv - Cell Biology 2022Quote: ... cells were resuspended in 20 µL of [1 mg/mL] 100T zymolyase (cat. no. 08320932, MP Biomedicals or cat. no. 320932, VWR) and incubated at room temperature for 6-7 minutes ...
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bioRxiv - Neuroscience 2022Quote: ... chloroform was added at a ratio of 1:5 (chloroform:trizol) for aqueous separation of RNA using heavy gel phase-lock tubes (VWR, 10847-802). Library preparation and RNA-sequencing was performed by Genewiz (Azenta Life Sciences) ...
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bioRxiv - Biophysics 2023Quote: ... The cell constants for individual electrode devices and buffer conductivities were measured using a lock-in amplifier as previously described and a 500 μS cm−1 conductivity standard (VWR, UK).14 The electrophoresis data was analysed using software written in Python.17 Linear fits to the slope of the plots of velocity against electric field ...
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bioRxiv - Neuroscience 2023Quote: ... and 1 mL of supernatant was transferred to a borosilicate glass culture tube pre-cleaned with HPLC-grade methanol (VWR International). Then 1mL of EA was added to the remaining sample (to maximize extraction efficiency) ...
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bioRxiv - Molecular Biology 2023Quote: ... PCR products were visualised on a 1% agarose gel (run for 30 minutes at 135 V) with GelGreen (VWR, 730-1535) using an ImageQuant™ LAS 500 (GE Lifesciences) ...
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bioRxiv - Synthetic Biology 2023Quote: ... The cells were then washed with 25 mL of ice cold STM buffer (pH = 7.5, 270 mM sucrose, Millipore Sigma, 1.07651.5000, 10 mM Tris HCl, Alfa Aesar, J67233, 1 mM MgCl2, VWR, E525-100ML) and spun down again at 4°C for 5 minutes at 1500 x g ...
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bioRxiv - Plant Biology 2023Quote: ... The seeds on their replum were then put on a slide with a drop of clearing solution (1 vol glycerol/7 vol chloral hydrate liquid solution, VWR Chemicals). The seeds were detached from the replum and the slide was covered with a coverslip and kept in the dark for at least 24h in a cold room (4°C) ...
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bioRxiv - Biophysics 2023Quote: ... and 1%(v/v) ammonium persulfate (APS) for 60 minutes at RT on plasma cleaned glass cover slips (No.1, 25mm Ø, VWR) that were pre-treated with 3-aminoproyltriethoxysiliane (APTES ...
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bioRxiv - Developmental Biology 2023Quote: ... for 50 ms at 1 s intervals using electrodes (diameter 3 mm; Sonidel CUY650P3) and ECM-830 BTX square wave electroporator (VWR international). After electroporation ...
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bioRxiv - Genomics 2023Quote: ... then incubated on rollers at room temperature for 1 hour with an anti-mouse secondary antibody conjugated to horseradish peroxidase (VWR, NXA931). After four further washes for 10 min each (2 x blocking buffer ...
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bioRxiv - Immunology 2023Quote: ... Gametes were washed with PBS and incubated with mAbs dilutions in PBS containing 0.5% PBS and 0.05% NaN3 (SIFA buffer) for 1 hr at 4 °C in sterile V bottom plates (VWR#736-0223). After incubation ...
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bioRxiv - Bioengineering 2019Quote: ... The Jurkat cell line (Clone E61, ATCC® TIB 152™) was obtained from ATCC and maintained in Complete RPMI medium: RPMI 1640 (VWR, Radnor, PA) + 25 mM dextrose ...
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bioRxiv - Cancer Biology 2020Quote: Primary OSE cultures and the OSN2 cell line (Flesken-Nikitin et al. 2003) were maintained in culture in media containing DMEM (VWR; Cat#: 10-017-CM), Hams F12 (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... was sterilized overnight again before being was sonicated in a Vibra Cell VC 750 sonicator equipped with a Cup Horn probe (VWR, Fontenay-sous-Bois, France) with the following settings ...
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bioRxiv - Cell Biology 2022Quote: ... 1 μL cell suspension was loaded on YES agarose pad (2% agarose) and covered with 22 x 22 mm glass coverslip (VWR #631-0125, thickness: 1.5). Time-lapse imaging of mNG-cdc8 cells were performed at 30 °C with acquisition of 16 z-slices ...
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bioRxiv - Immunology 2022Quote: Splenic T-lymphocytes were counted and plated at an optimal seeding density of 8×104 cells per 100 µL in 96 well Microtitration ImageLock plates (VWR, #4379, Radnor, PA, USA). The cells were cultured with anti-CD3 and anti-CD28 activation beads and imaged over the course of 96hrs in the Essen Bioscience IncuCyte S3 Live-Cell imager and analysis system.
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bioRxiv - Microbiology 2022Quote: ... African green monkey kidney cells (Vero) were maintained in DMEM supplemented with 5% heat-inactivated fetal bovine essence (FBE) (VWR Life Sciences 10803-034), 1% Penicillin/Streptomycin (Gibco ...
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bioRxiv - Genomics 2023Quote: ... The resulting plasmid libraries were purified and concentrated via SPRI bead cleanup before being transformed into electrocompetent cells (Lucigen Endura, VWR International LLC #71003-038) for plasmid library amplification ...
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bioRxiv - Biophysics 2024Quote: ... 293T/17 cells were plated in complete DMEM at 8 × 105 cells per well in a 6-well plate with an autoclaved glass coverslip (VWR, no. CLS-1760-025) to ensure 80% confluency on the day of the experiment ...
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Synaptonemal Complex dimerization regulates chromosome alignment and crossover patterning in meiosisbioRxiv - Cell Biology 2020Quote: ... pSUN-1::TIR-1::mRuby him-8(tm611) spo-11-aid IV (ROG212) adult hermaphrodites were grown on 1 mM auxin (indole-3-acetic acid, VWR AAA10556-36) and therefore lacked SPO-11 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and was subsequently propagated in a well of an unshaken flat-bottom polypropylene 96-well plate in one of three conditions: YPD (1% Bacto yeast extract (VWR #90000–726), 2% Bacto peptone (VWR #90000–368) ...
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Phenotypic and molecular evolution across 10,000 generations in laboratory budding yeast populationsbioRxiv - Evolutionary Biology 2020Quote: ... we plated a 150 μL of 1:100 diluted saturated culture of a sensitive strain (YAN563) on a single-well (VWR #46600-638) methylene blue agar plate (20 g/L peptone ...