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Citations for VWR :
1351 - 1400 of 1558 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2022Quote: ... Optical density (OD) was measured in technical triplicates in 1-mL polystyrene cuvettes in a V-1200 spectrophotometer (VWR) at 600 nm ...
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bioRxiv - Microbiology 2022Quote: ... was diluted 1:3 in cold PBS and 100 μL was added to 0.4 μm polycarbonate trans-well plate inserts (VWR). The Transwell plates were placed 4°C for 30 min to let the Matrigel settle and were then moved to an anaerobic chamber to gel at 37°C for 24 h ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... washed twice in distilled water for 2 minutes and dehydrated through 70% and 95% ethanol (4.5 and 1 minute respectively) before being incubated in Histoclear (VWR) for 3 minutes ...
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bioRxiv - Microbiology 2022Quote: ... The pellet was resuspended in 1 mL of cold brain heart infusion (BHI) broth (VWR, cat. no. 90003-040) per infected T175 cm2 flask ...
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bioRxiv - Neuroscience 2022Quote: ... chloroform was added at a 1:5 volumetric ratio (chloroform:Trizol) for aqueous separation of RNA via centrifugation in heavy gel phase-lock tubes (VWR). Aqueous phase was transferred into isopropanol followed by RNA purification using a Qiagen RNeasy Mini Kit as per manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were then rinsed 3x in TBS and incubated for 1 hour in anti-mouse IR680 (VWR 102971-020) and anti-rabbit IR800 (VWR 103011-494 ...
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bioRxiv - Neuroscience 2023Quote: ... 1:2 dilution) was injected into the three DCN using a Micro4 controller and UltraMicroPump 3 (VWR, Radnor, PA). Glass needles were made from 1-mm outer diameter glass pipettes (Wiretrol II ...
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bioRxiv - Physiology 2023Quote: Post-ultracentrifugation supernatant (S2) from brain lysates were thawed on ice alongside 1 mM Thioflavin T (VWR 103802-652). Thioflavin T (ThT ...
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bioRxiv - Microbiology 2023Quote: ... XTT [2,3-bis-(2-methoxy-4-nitro-5-sulfophenyl)-2H-tetrazolium-5-carboxanilide] solution (0.5 mg XTT/mL 1× PBS with 25 µM menadione) (XTT sodium salt, VWR) was added at 150 µl per well and incubated at 37 °C ...
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bioRxiv - Cell Biology 2022Quote: ... THP-1 monocytes were differentiated into macrophages by treatment with 50 nM phorbol 12-myristate 13-acetate (PMA, VWR) for 3 days at a density of 5 × 104 cells per well in a 96-well tissue culture plate (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... 1:10,000 NaOH 10N, 0.02% sodium azide) for 1 hour for each step (20%, 40%, 60%, 80%) on a nutator (VWR). Samples were then washed with 100% MeOH 2x for 1hr each and then incubated in a 2:1 dicholoromethane (DCM):MeOH solution overnight ...
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bioRxiv - Microbiology 2023Quote: ... afra leaf material was ground and extracted in dichloromethane (DCM) (1 g:20 mL, VWR International, Radnor, PA, USA) under sonication for 30 minutes ...
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bioRxiv - Neuroscience 2023Quote: ... washed twice in distilled water for 2 minutes and dehydrated through 70% and 95% ethanol (4.5 and 1 minute respectively) before being incubated in Histoclear (VWR) for 3 minutes ...
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bioRxiv - Microbiology 2023Quote: ... Culture supernatant containing soluble gp120 glycoprotein was then clarified by centrifugation at 7000 x g for 1 hour and filtered using a 0.45 μm vacuum filtering system (VWR). Supernatant was dialyzed against Ni-NTA buffer (50 mM NaH2PO4 ...
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bioRxiv - Cell Biology 2023Quote: ... membranes were incubated for 1-2 hours at RT with primary antibodies diluted in Odyssey Blocking Buffer + 0.2% Tween (VWR). Next ...
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bioRxiv - Synthetic Biology 2023Quote: ... 10 mL of seed culture was used to inoculate 1 L media in a Fernbach flask (VWR 29171-854). Cells were incubated at 37 °C shaking at 210 rev/min ...
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bioRxiv - Neuroscience 2024Quote: ... samples were homogenized with a tissue grinder dounce (WHEATON® Dounce Tissue Grinder 1 mL, VWR, Radnor, Pennsylvania, USA) in 150 mM NaCl (S3014 Sigma-Aldrich - Merck ...
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bioRxiv - Cell Biology 2020Quote: ... 5-day differentiated BMDMs isolated from control LysM-Cre+ or LysM-Cre+ Atg16L1loxp/loxp mice were plated at 10 x 106 cells per 15-cm non-TC treated dish (Petri dish, VWR, 25384-326) in BMDM media ...
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bioRxiv - Developmental Biology 2021Quote: ... for duck fibroblasts (i.e., CCL-141 cells) supplemented with 10% Fetal bovine serum (FBS, 97068-085, Lot# 283K18, VWR, Radnor, PA, USA) and 1X penicillin-streptomycin (15140122 ...
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bioRxiv - Evolutionary Biology 2021Quote: ... and cells were resuspended in 65μL RNAase A solution consisting of 10 mg/mL RNAase A (VWR Life Science, 9001-99-4) dissolved in 10 mM Tris-HCl ...
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bioRxiv - Systems Biology 2020Quote: ... cells were removed from their liquid media by flowing the liquid cultures that contained cells through a 0.45−μm pore filter (VWR, cellulose-acetate membrane). To ensure and verify that there were no cells remaining in the filtered media ...
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bioRxiv - Systems Biology 2020Quote: ... all subpools were combined into the final mutant collection pool by pipetting the subpools through a 100 μm cell strainer (VWR 10054-458). The final pool was mixed using a magnetic stir bar ...
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bioRxiv - Cancer Biology 2020Quote: 1.2 × 105 cells of HF3016 were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.2 × 105 cells of HF3016 were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Cancer Biology 2020Quote: 3 × 105 cells of HF3016 were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-742). The next day ...
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bioRxiv - Cancer Biology 2020Quote: ... 1.2 × 105 cells were plated with 10% FBS-containing NMGF media into a glass-viewing area of confocal dish (VWR, 75856-740). The next day ...
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bioRxiv - Genomics 2021Quote: ... and myotubes from one 10 cm plate (∼5 million cells) with >90% viability were lifted as described above and pelleted in 15 mL polypropylene falcon tubes (VWR, 89039-670) by centrifuging for 5 minutes at 1500 RPM ...
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bioRxiv - Biochemistry 2021Quote: ... A total of 2000 cells per well in a volume of 100 µl were seeded on flat-bottomed 96-well plates (VWR, Radnor, USA) and incubated in a 95% humidified atmosphere with 5% CO2 at 37 °C for 24 h ...
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bioRxiv - Developmental Biology 2020Quote: ... for duck fibroblasts (i.e., CCL-141 cells) supplemented with 10% Fetal bovine serum (FBS, 97068-085, Lot# 283K18, VWR, Radnor, PA, USA) and 1X penicillin-streptomycin (15140122 ...
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bioRxiv - Synthetic Biology 2022Quote: ... Each plant was sprayed for one minute and the collected flow through from each plant were first strained using a 40 μm cell strainer (VWR 76327-098) to remove large microorganisms and large particles ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then washed twice with mitochondrial assay solution (220mM mannitol [Sigma-Aldrich, M4125], 70mM sucrose [Sigma-Aldrich, S7903], 10mM KH2PO4 [VWR, 470302] ...
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bioRxiv - Molecular Biology 2023Quote: ... 1-2 preps of in vitro transcribed RNA and 5-10µg N gene RNA were mixed with the prepared cell suspension in a 4mm electroporation cuvette (VWR International # 89047-210) and pulsed in a BioRad GenePusler using the exponential protocol with the following settings ...
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bioRxiv - Cell Biology 2022Quote: The lipidomics solubilization buffer used to resuspend whole-cell lysates and GolgiTAG immunoprecipitates was made of LC/MS grade chloroform (VWR. Cat# 22711.260) and Optima LC/MS grade methanol (Fisher ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... when confluence was reached and counting/replating using Trypan blue exclusion dye in an automatic cell counter (EveTM, NanoEnTek distributed by VWR, Milan, Italy) in accordance with the manufacturer’s instructions.
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bioRxiv - Cell Biology 2023Quote: ... After a PBS wash 500 µl TRIzol was added to the cells followed by the addition of 300 µl chloroform in a heave gel phase-lock tube (VWR, 10847-802) for phase separation using centrifugation ...
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bioRxiv - Bioengineering 2024Quote: ... The cells collected from the BM and spleen were filtered through 70 μm mesh and enriched for haemopoietic lineage cells by density centrifugation using Lympholyte-Mammal (VWR, Lutterworth, UK), centrifuging the cell suspension for 20 min at 625 g at RT ...
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bioRxiv - Genomics 2024Quote: Total RNA of minigene-transfected HEK293T cells and patient-derived fibroblasts was extracted using the peqGOLD Total RNA Kit (VWR Life Science). One microgram of RNA was treated with 1 U of DNaseI (Sigma-Aldrich ...
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bioRxiv - Biophysics 2021Quote: ... the lipid mixture was resuspended in 1 ml of PBS and sonicated for 10 minutes (ultrasound bath USC500TH, VWR, England) to form small unilamellar vesicles (SUVs) ...
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bioRxiv - Developmental Biology 2022Quote: ... Ganglioid frozen sections and paraffin-embedded human normal colon sections were prepared as above and then washed three times in PBS and blocked for 1-2 hours in serum (10% donkey or 10% goat) with 0.5% (v/v) Triton X-100 (VWR 0694). Slides were then incubated with primary antibody diluted in serum (10% donkey or 10% goat ...
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bioRxiv - Neuroscience 2019Quote: ... was made with a dental drill (Osada) for injection into the target (see Table 1) using a calibrated glass micropipette (VWR) at a rate of 0.3–0.5 μl/min ...
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bioRxiv - Biochemistry 2020Quote: ... All PKC assays (except the PKC-mu and the PKC-nu assay) additionally contained 1 mM CaCl2 (VWR; Cat. # 1.02382), 4 mM EDTA (Roth ...
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bioRxiv - Microbiology 2019Quote: ... Pellets were thawed on ice and resuspended in 250 μL of PBS supplemented with 1 M NaCl and 6 M Urea (VWR). The resuspended bacteria were then added to screw cap microcentrifuge lysing tubes (Fisher Scientific ...
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bioRxiv - Biochemistry 2019Quote: HDL samples prepared by adding 15 μL 3 mg/mL spin-labeled apoA-I probe to 45 μL 1 mg/mL HDL and drawn into an EPR-compatible borosilicate capillary tube (VWR) (32) ...
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bioRxiv - Bioengineering 2020Quote: ... SiO2 particles were washed with methanol and centrifuged for 1 min to remove supernatant and then again mixed with methanol and vortexed (Vortex mixer, VWR) prior usage ...
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bioRxiv - Microbiology 2021Quote: Gametocytes were produced by treatment of the mice at 1% parasitemia with 10 mg/L sulfadiazine (VWR, Cat# AAA12370-30) in their drinking water for two days before exsanguination ...
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bioRxiv - Physiology 2021Quote: ... with 2 mL freshly prepared TST buffer (0.03% Tween 20 [Bio-Rad], 0.01% Molecular Grade BSA [New England Biolabs], 146 mM NaCl [ThermoFisher Scientific], 1 mM CaCl2 [VWR International] ...
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bioRxiv - Cell Biology 2021Quote: ... resuspended in ~100 μL of culture supernatant and 3 μL deposited on a pre-set 65 μL drop of SC+ 2% glucose+ 2% agar gel medium on a circular glass coverslip (#1, VWR) that was encircled by an adhesive silicon ring ...
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bioRxiv - Neuroscience 2020Quote: ... and then cryoprotected for 3 days at 4 °C in 30% sucrose solution (w/v in 1× PBS; VWR Chemicals). Brains were then embedded into optimal cutting temperature (OCT ...
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bioRxiv - Microbiology 2021Quote: ... Mice were culled 1 day later and their spleens were collected in optimal cutting temperature (OCT) compound (VWR Chemicals, UK) and stored at -80° C to test for the presence of FDC-associated IC by confocal microscopy.
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bioRxiv - Molecular Biology 2021Quote: ... The patterned PDMS chip was then plasma bonded to a 52 mm x 76 mm x 1 mm (length x width x thickness) glass slide (VWR) in a low-pressure oxygen plasma generator (Femto ...