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Citations for VWR :
1201 - 1250 of 1692 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... All cell lines were tested for mycoplasma using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination.
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bioRxiv - Neuroscience 2024Quote: ... the overlay media was removed and cells were fixed/stained using 10% neutral buffered formalin (VWR #89370) and 0.25% crystal violet (VWR #0528 ...
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bioRxiv - Neuroscience 2024Quote: ... Cells were maintained in feeder-free culture conditions on Nunc tissue culture-treated 6-well plates (VWR) coated with Matrigel (Corning ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were washed thrice with PBS and coverslips were mounted on microscopy slides (VWR, Radnor, PA, USA) with either DAKO fluorescent mounting medium (Agilent ...
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bioRxiv - Immunology 2021Quote: ... The reaction was terminated with 50μL of 1% (w/v) SDS (VWR International, Radnor, PA, USA). Colorimetric absorbance was measured at 414nm using a PowerWaveXS plate reader (Biotek ...
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bioRxiv - Developmental Biology 2020Quote: ... the NTM was replaced with 1 mL NTM with 0.25 mg/mL NBT (VWR, 97061-412) and 0.125 mg/mL BCIP ...
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bioRxiv - Genomics 2020Quote: ... 3 15min washes in 5x SSCT (1:10,000 DAPI, VWR, TCA2412-5MG, in the second wash), and storage/imaging in 5x SSCT ...
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bioRxiv - Biophysics 2020Quote: ... PDMS (base to curing agent ratio of 10:1 w/w; #634165S, SYLGARD 184, VWR, Germany) was poured over the master ...
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bioRxiv - Bioengineering 2021Quote: ... and secondary antibodies were diluted to 1:10,000 (100 ng/mL) in 2.5% BSA (0332, VWR) in Tris buffered saline tween ...
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bioRxiv - Bioengineering 2022Quote: ... 350μL of a 40mg/mL−1 solution of bovine serum albumin (BSA) (AAJ64100, VWR, PA, USA) in PBS (Thermo Fisher ...
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bioRxiv - Evolutionary Biology 2019Quote: 1 ml of each lysate was first treated with 12.5 μl 1M MgCl2 (VWR Life Science), 0.4 μl DNAse 1 (2000 U/ml ...
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bioRxiv - Bioengineering 2020Quote: ... 1-(3-(Dimethylamino)propyl)-3-ethylcarboiimide hydrochloride (EDC-HCL; 0.5 g, 2.6 mmol, 1.02 equiv., VWR) and NHS-ester (0.65 g ...
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bioRxiv - Microbiology 2021Quote: ... PCR products were loaded on a 1% agarose gel and stained with EZ-Vision® (VWR). Images were taken using the GelDoc system (Bio-Rad).
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bioRxiv - Molecular Biology 2021Quote: ... rose tissue was macerated in 1 mL of phosphate buffer saline (1X PBS) (VWR, Radnor, PA), 0.05% Tween 20 ...
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bioRxiv - Genomics 2021Quote: ... 3 15min washes in 5x SSCT (1:10,000 DAPI TCA2412-5MG, VWR in the second wash), and storage/imaging in 5x SSCT ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... transferred to an ~1 cm square agar “pad” on a glass microscope slide (VWR; 16004-422) and covered with a glass coverslip (VWR ...
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bioRxiv - Microbiology 2019Quote: ... in tap water (pH adjusted to 7.2 with NaOH) + 1% (w/v) glucose (VWR International, USA) containing 100 μg/mL ampicillin (E ...
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bioRxiv - Physiology 2022Quote: ... Total RNA (1 μg) was reverse transcribed using qScript cDNA Synthesis Kit (VWR, Houston, TX, USA). qRT-PCR analysis was performed using SYBR Green qPCR Master Mix (VWR) ...
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bioRxiv - Immunology 2022Quote: ... The reaction was terminated with 50μL of 1% (w/v) SDS (VWR International, Radnor, PA, USA). Colorimetric absorbance was measured at 414nm using a PowerWaveXS plate reader (Biotek ...
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bioRxiv - Synthetic Biology 2023Quote: ... then plated onto YPM supplemented with 60 µg mL-1 5-fluorocytosine (5-FC) (TCF0321, VWR). Colonies that emerged were then transferred onto a new YPM 5-FC agar plate for clonal isolation ...
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bioRxiv - Cancer Biology 2023Quote: ... the peptides were re-dissolved in 10 μl 1% formic acid (VWR Ltd., Radnor, PA, USA) and were separated in a 180 min water/acetonitrile gradient using an Easy nLC 1200 nano UPLC (Thermo Scientific ...
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bioRxiv - Neuroscience 2023Quote: ... RRID:AB_2259284) in a 1:500 dilution in PB containing 0.4% Saponin (VWR International AS, Bergen, Norway) and 5% normal goat serum ...
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bioRxiv - Microbiology 2024Quote: ... the extracted biofilm matrix was lyophilized using an ALPHA 1-4 LDplus freeze dryer (Christ, VWR) and kept at −80 °C until sample preparation for proteomics.
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bioRxiv - Microbiology 2024Quote: ... Pellet was resuspended in 1 mL of Dulbelcco’s Modified Eagle’s Medium (DMEM) (Corning; VWR 45000-304). This suspension was diluted 10-fold by adding 500 μL to 4.5 mL of DMEM to a final concentration of 1×106 cells/mL ...
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bioRxiv - Neuroscience 2024Quote: ... xvissem is the binary semantic feature encoding visual semantics (VN = 1, AN, VWR, and AWR = 0). For analysis in Fig ...
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bioRxiv - Cancer Biology 2021Quote: Cells were treated for 4 h with 4HC or doxorubicin at IC50-range drug concentrations using cells seeded the prior day at 50,000 (E0771) or 75,000 cells per well (4T1, B16F10) of a 6-well plate (cat. #10861-696, VWR). Cells were then washed once with PBS and incubated in fresh media for a total of 24 ...
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bioRxiv - Genetics 2020Quote: ... the egg-containing plug-in cells were removed from the plates and fixed vertically on petri dishes (VWR) with plastiline (Pelikan Schindellegi ...
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bioRxiv - Molecular Biology 2021Quote: ... we transfect the cells with either WT or mutant SOD1 pCI-Neo plasmid using JetOptimus transfection reagent (VWR) according to the manufacturer’s instructions (31) ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μL of cell suspension were loaded under a 22 x 22 mm glass coverslip (VWR, thickness: 1.5). Spinning disk confocal images of S ...
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bioRxiv - Cell Biology 2019Quote: ... Equal spotting was controlled by simultaneously spotting cells using a multi-channel ultra-high-performance pipette (VWR International). Cells were grown on selective plates at 30°C for 2 days and imaged using a Geldoc system (Bio-RAD) ...
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bioRxiv - Immunology 2019Quote: ... or with 5×104 B16F10 wild type (WT) or B16F10-Globin-SL8-intron cells along with matrigel (VWR). Five days after challenge ...
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bioRxiv - Neuroscience 2021Quote: ... The cortex was dissected and homogenized into a single cell suspension using a tissue homogenizer (5 cm3, VWR) and filtered m strainer (Falcon) ...
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bioRxiv - Cell Biology 2021Quote: An early passage iMPC clone was trypsinized and filtered using a 40 μm cell strainer (VWR, 734-0002) to filter out debris and fragments of myofibers ...
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bioRxiv - Synthetic Biology 2021Quote: ... HEK293T and HEK293TERK2-EGFP cells were cultured in DMEM media supplemented with high glucose and L-glutamine (VWR) supplemented with 10% Hyclone FetalClone I serum (VWR ...
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bioRxiv - Microbiology 2020Quote: ... The flow cell was pre-incubated with microscopy medium and a bottom coverslip (25 mm No. 1.5; VWR) was attached to the cell ...
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bioRxiv - Cancer Biology 2020Quote: Cell line MDA MB 436 was propagated in DMEM media supplemented with 10% FBS (VWR Seradigm Life Science), 1% L-glutamine and 1% sodium pyruvate (Life Technology-Thermo-Fisher) ...
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bioRxiv - Molecular Biology 2020Quote: ... Cell pellets were then washed 1x with PBS and resuspended in RIPA lysis buffer (VWR, cat # 97063-270) supplemented with protease (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... purified cells were diluted and imaged at 100x magnification within a 20 μm counting chamber (VWR 15170-048). Images were analyzed using FIJI/ImageJ 1.52p (Java 1.8.0_172 ...
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bioRxiv - Immunology 2020Quote: ... 2.5 × 104 target cells were pipetted per well into 96-well black plates with clear-bottom (VWR/Corning) and kept in the dark for 20 min at room temperature ...
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bioRxiv - Genetics 2020Quote: ... after which the media was changed to complete growth medium and the cells expanded in T182.5 flasks (VWR) in monolayer and until confluent (between 10-14 days) ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... HEK T-REx cells were transfected with pcDNA4/TO/hTRPC3 using jetPRIME ® transfection reagent (VWR, Lutterworth, UK) according to manufacturer’s instructions ...
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bioRxiv - Biophysics 2021Quote: ... For TPE-FLIM imaging the cells were seeded on 18 mm diameter microscope cover glass (VWR, Darmstadt, Germany) in PBS containing Ca2+ at room temperature.
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bioRxiv - Cancer Biology 2022Quote: ... the cells were then washed with PBS thrice and treated with 1.6 mM sodium metaperiodate (VWR, 13798-22) for 20 minutes at 4C for oxidation of the N-linked sugar residues ...
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bioRxiv - Cancer Biology 2022Quote: ... cell proliferation was assessed using a 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, VWR, PA, USA) assay ...
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bioRxiv - Microbiology 2022Quote: ... The supernatant was aspirated off and the cells were resuspended in 500 μl RiboZol RNA extraction reagent (VWR). Tubes were then vortexed and 100 μl chloroform was added to the tube ...
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bioRxiv - Plant Biology 2022Quote: ... The latter was captured in a 300 mL beaker and poured through a cell strainer (VWR, Radnor, USA) with a nominal pore size of 100 µm ...
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bioRxiv - Microbiology 2022Quote: ... cells from a rocking culture of swimmers were added to 60 mm non-treated tissue culture plates (VWR) in 2 mL of BHI ...
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bioRxiv - Microbiology 2024Quote: ... infected HCT-8 cells in 96-well plates were fixed with sterile 4% paraformaldehyde (PFA; VWR Chemicals #28794.295) in PBS for 10 minutes at room temperature ...
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bioRxiv - Physiology 2024Quote: ... cells were perfused with Ca2+-containing Krebs-Ringer’s solution supplemented with 0.1% fatty-acid free BSA (E588, VWR). To trigger ionic responses ...
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bioRxiv - Microbiology 2024Quote: ... Bacterial cells were separated from the culture liquid by centrifugation and filtration through 0.22 µm membranes (VWR, USA). Next ...