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Citations for VWR :
1401 - 1450 of 1692 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... HAF109) were incubated for 1 h prior to detection with SuperSignal West Pico Plus Chemisubstrate (VWR International, PIER34577).
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bioRxiv - Synthetic Biology 2020Quote: ... 10 µL was quickly pipetted onto a 20 x 20 mm #1 cover glass (VWR, Radnor, PA, USA) and inverted onto a glass slide before the gelation reaction was completed ...
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bioRxiv - Neuroscience 2020Quote: ... Whole brains were then incubated in a solution of 1:2 30% Sucrose:OCT Compound (Tissue-Tek, VWR Canada) for 30 min ...
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bioRxiv - Biochemistry 2020Quote: ... Blocks of around 1 mm2 were cut and cryo-protected by overnight infiltration in 2.3 M sucrose (VWR) in 0.1 M phosphate buffer ...
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bioRxiv - Cell Biology 2021Quote: ... Reduction of temperature to 25 °C and induction recombinant gene expression by addition of 1 mM IPTG (VWR) was triggered automatically by pH (7.1) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... We filter-sterilized the supernatants by transferring them to 96-well 1 mL filter-plates (VWR International, Switzerland), followed by centrifugation at 1200 rpm for 15 minutes ...
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bioRxiv - Bioengineering 2021Quote: ... The samples were then permeabilized in 1× PBS with 0.25% TritonTM X-100 (Cat. No. 97063-864, VWR) and FBS for 20 min ...
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bioRxiv - Biochemistry 2020Quote: α-Syn (1 mg/mL) was dissolved in 10 mM NaOH and sonicated (VWR® Symphony™ Ultrasonic Cleaner) for 20 minutes ...
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bioRxiv - Bioengineering 2022Quote: ... Samples (1 μL) were added to reaction mixtures (19 μL) in a 96-well white PCR plate (VWR) on ice ...
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bioRxiv - Cell Biology 2022Quote: ... were grown to O.D 0.3-0.6 and incubated with non-fluorescent Halo tag ligand 7-bromo-1-heptanol (7BRO, 100μM, VWR #AAH54762) for 10 min to irreversibly mask all the pre-existing ribosomal proteins ...
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bioRxiv - Developmental Biology 2023Quote: ... mice were weighed 1 hr prior to harvest and injected with 0.04 µmol/g puromycin (VWR, #75844-852).
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bioRxiv - Cell Biology 2022Quote: ... 1 ml of conditioned medium was combined with 395 μl of premixed TCA (256 µl VWR Life Science) and 1% Triton-X 100 (139 µl ...
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bioRxiv - Neuroscience 2024Quote: ... xaudsem is the binary semantic feature encoding auditory semantics (AN = 1, VN, VWR, AWR = 0, see Fig. 2A). xvissem is the binary semantic feature encoding visual semantics (VN = 1 ...
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bioRxiv - Molecular Biology 2024Quote: ... and subjected to electrophoresis at 180 volts for 50 minutes in a preformulated 1× SDS-PAGE buffer (VWR). Proteins were then electrotransferred onto pre-activated polyvinylidene difluoride membranes (Bio-Rad ...
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bioRxiv - Neuroscience 2024Quote: ... The supernatant was extracted against an equal volume of phenol:chloroform:iso-amyl alcohol (25:24:1) (VWR, 97064-692). The upper aqueous phase was then precipitated from ethanol as described above ...
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bioRxiv - Cell Biology 2020Quote: Protein extracts from total cells and eluates from immuno-precipitations were separated by standard SDS-PAGE and transferred to PVDF membranes (#10600023, VWR, USA) for the detection of Mup1-GFP ...
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bioRxiv - Cell Biology 2022Quote: Coverslips with plated cells were rinsed with GLOX/BME buffer and mounted onto single cavity glass slides (VWR, cat #10118-600), pre-filled with 80 μl GLOX/BME buffer immediately prior to the placement of coverslip ...
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bioRxiv - Genetics 2019Quote: HEK 293T cells were cultured in six-well plates and double transfected using jetPRIME transfection reagent (VWR International, Radnor, PA, USA) with pCMV-luciferase ...
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bioRxiv - Bioengineering 2021Quote: ... cell suspensions were prepared at a concentration of 300,000 cells/mL in 1X phosphate buffered saline (PBS) solution supplemented with 2% fetal bovine serum (FBS, VWR 89510-186) to reduce cell-cell and cell-PDMS adhesion ...
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bioRxiv - Biochemistry 2021Quote: ... FOCUS and HuH-7 cells were grown in Dulbecco’s minimum essential medium (DMEM) supplemented with 10% FBS (VWR Life Science, Seradigm). C3A ...
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bioRxiv - Microbiology 2021Quote: ... The pellet was resuspended in 15.3 mL sterile saline and filtered with a Falcon Cell Strainer 100 μm Nylon Filter (VWR, Dietikon, Switzerland) to remove particles ...
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bioRxiv - Microbiology 2021Quote: ... Optical density was followed over the course of the experiment by measuring it directly from the Hungate tube with a Biowave cell density meter CO8000 (VWR International). Similarly ...
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bioRxiv - Cancer Biology 2022Quote: ... The bones are then crushed with a pestle and washed and passed through a 40 µm cell strainer (VWR, 76327-098). RBC lysed and dead cells were separated (using dead cell removal kit ...
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bioRxiv - Neuroscience 2022Quote: ... The tissue was then triturated in 10ml of warm growth medium and passed through a 70μm cell strainer (VWR #10199-657) to remove large particulates ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were plated in 6-well plates at 1 million cells per well and the following day were transfected with 2 μg DNA using 6 μl of JetPrime (VWR International) according to the manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: ... Nuclei were isolated through dounce-homogenization followed by filtration with a 70uM cell strainer (cat no. 21008-952, VWR, Radnor PA). The following antibodies were used to immunotag nuclei ...
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bioRxiv - Cancer Biology 2021Quote: MCF7 KRT19 KO cells that were generated using the CRISPR/Cas9 system (Sharma et al., 2019a) and isogenic parental control cells (ATCC, Manassas VA) were grown in Dulbecco’s Modified Essential Medium (VWR Life Science) containing 10% fetalgro bovine growth serum (RMBIO ...
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bioRxiv - Cancer Biology 2019Quote: ... FOCUS and HuH-7 cells were grown in Dulbecco’s minimum essential medium (DMEM) supplemented with 10% FBS (VWR Life Science, Seradigm). C3A ...
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bioRxiv - Molecular Biology 2020Quote: ... or a non-specific siGENOME Non-Targeting siRNA (Pool #2, Dharmacon) were transfected into U-2 OS cells with INTERFERin™ Polyplus Reagent (VWR) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2019Quote: ... mice were reconstituted 4 hrs post-irradiation with 5 million donor bone marrow cells in 200 μl Hank’s Balanced Salt Solution (HBSS; Lonza, distributed by VWR, Lutterworth, UK), administered by intravenous injection into the tail vein ...
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bioRxiv - Bioengineering 2021Quote: ... BM-MSCs isolated from heparinized bone marrow aspirates by seeding 50,000 white blood cells/cm2 on two-chamber CellStacks (Corning/VWR, Ulm, Germany) in α-MEM (Lonza ...
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bioRxiv - Genetics 2020Quote: ... Nuclei were isolated by dounce-homogenization followed by filtration through a 70uM cell strainer (cat no. 21008-952, VWR, Radnor PA). To immunotag cell type specific nuclei ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were cultured in DMEM (PAN-Biotech GmbH, Aidenbach, Germany) supplemented with 10% (v/v) fetal bovine serum (VWR International GmbH) and 0.2% penicillin-streptomycin (PAN-Biotech) ...
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bioRxiv - Cancer Biology 2024Quote: A total of 5 × 106 MM.1Sgfp+/luc+ cells were seeded in T-25 flasks (Avantor/VWR, Cat. No. 10861-568) and treated with the vehicle (DMSO ...
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bioRxiv - Cancer Biology 2024Quote: ... Lentivirus-containing medium was collected from HEK293T cells 48h and 72h post-transfection and added to recipient cancer cells after filtration using a 0.45 µM filter (VWR-Corning, 431220). 48h post infection ...
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bioRxiv - Physiology 2023Quote: ... 8 μl of the final polyacrylamide mix were pipetted on 35 mm glass bottom dishes (Cell E&G LLC VWR, USA) which were pretreated with 0.1 M NaOH ...
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bioRxiv - Neuroscience 2024Quote: ... We obtained HEK-293 cells from Jong Yun Lab (Penn State College of Medicine) and cultured them in DMEM (VWR International) supplemented with 10% fetal bovine serum (Fisher Scientific) ...
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bioRxiv - Developmental Biology 2023Quote: ... NPCs were plated in 24 well CELLSTAR® cell culture plates (for bulk-RNA-seq. and live imaging; VWR, 82050-892), µ-Slide 8 Well (for Video S1. ...
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bioRxiv - Molecular Biology 2023Quote: ... TSC feeder-dependent cells were cultured on mitomycin-treated MEFs at a 40-60% density in RPMI1640 (VWR, 10-040-CV) supplemented with 20% fetal bovine serum (GIBCO ...
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bioRxiv - Neuroscience 2023Quote: ... and cryostat-sectioned at 14Lµm horizontality (capturing the ganglion cell layer of the whole retina) onto Superfrost Plus glass slides (VWR International, LLC). For immunostaining ...
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bioRxiv - Biochemistry 2022Quote: Capsaspora owczarzaki cell cultures (strain ATCC®30864) were grown axenically in 25 cm2 culture flasks (#734-0044, Falcon® VWR) with 5 mL ATCC media 1034 (modified PYNFH medium ...
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bioRxiv - Immunology 2023Quote: ... were cultured in six-well plates at a density of 5 x 106 cells/mL in 2 mL of RPMI 1640 (cat#45000-396, VWR, USA) supplemented with 10% fetal bovine serum (Omega Scientific ...
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bioRxiv - Neuroscience 2024Quote: ... S2 cells were grown at 22°C in Schneider’s Drosophila medium supplemented with 10% Fetal Bovine Serum (FBS) (VWR, Radnor, PA) and 0.5% Penicillin/streptomycin (Sigma-Aldrich) ...
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bioRxiv - Neuroscience 2021Quote: Tissue samples were lysed in NP40 buffer (1% NP40 in 10 mM Tris, pH 7.4, 150 mM NaCl) in a Precellys homogenizator (VWR) and 10 μg total protein per sample were proteolyzed with Lys-C and trypsin using a modified FASP procedure (71).
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bioRxiv - Neuroscience 2021Quote: ... the samples were resolubilized with 15 min sonication in 30 μL of 1% acetonitrile + 0.1% trifluoroacetic acid in LC-MS grade water (all from VWR). The injection volume (between 2 and 10 μL ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... by combining compounds with 0.5 μg/mL recombinant luciferase in 1x PBS buffer and 1 mg/mL BSA (VWR). Reactions were initiated by the addition of 30 μL luciferin substrate to wells ...
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bioRxiv - Cancer Biology 2021Quote: ... and 0.1% Fast green (Raymond A Lamb) combined in a 1:9 dilution with aqueous Picric acid solution (VWR).
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bioRxiv - Immunology 2021Quote: ... and sequentially washed with RIPA (PBS pH 7.4 with 0.5% sodium deoxycholate [Thermo], 0.1% sodium dodecylsulfate [Fisher Scientific, Waltham, MA], 1% Nonidet P-40 substitute [VWR]), high salt PBS (PBS pH 7.4 ...
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bioRxiv - Neuroscience 2021Quote: ... Plates were rocked every 15 minutes for 1 hour before a semi-solid overly containing 0.5% Methylcellulose in viral growth media (VWR) was applied ...
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bioRxiv - Synthetic Biology 2021Quote: ... the cultures were fixed by adding 2 μL culture into 198 μL 1 × phosphate-buffered saline (K813-500ML, VWR) in a 96-well U-bottom plate (Thermo Fisher Scientific ...