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Citations for VWR :
1301 - 1350 of 1692 citations for Dengue Virus Serotype 1 NS1 Protein Insect Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2019Quote: ... undifferentiated hPSCs were initially seeded at 1.0 million cells/1mL in ultra-low attachment 6 well plates (Corning, VWR) placed on a 9-Position stir plate (Chemglass ...
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bioRxiv - Immunology 2021Quote: Single-cell leukocyte suspensions were prepared from spleens by mechanical homogenization of tissues between frosted glass microscope slides (VWR) and filtration through a 70µm nylon mesh ...
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bioRxiv - Cancer Biology 2021Quote: ... Cell supernatants were collected at different time points and centrifuged 30min at 10,000 × g at 4°C to eliminate cell debris (centrifuge CT15RE, VWR), then at 100,000 × g for 3h to eliminate larger vesicles (L7-55 Ultracentrifuge ...
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bioRxiv - Bioengineering 2020Quote: RAW 264.7 macrophages were seeded at 50,000 cells per well in a 96 well black glass-bottom plate (Greiner 96 well plates, 655891, VWR). Cells had been previously incubated with folic acid deficient RPMI medium (10% FBS ...
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bioRxiv - Bioengineering 2020Quote: H1299 cells were seeded at 20,000 cells per well in a 96 well black glass-bottom plate (Greiner 96 well plates, 655891, VWR). Media was replaced with media containing treatments (1% DMSO ...
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bioRxiv - Cancer Biology 2020Quote: ... MSCs were plated at 1.75×105 cells per 4.2cm2 of a 6-well transwell insert (Falcon 353090, VWR, Germany).
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed once in water followed by mounting the coverslip onto glass slides with Vectashield (VWR, 101098-042) and finally sealing the coverslip with nail polish (Electron Microscopy Science Nm ...
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bioRxiv - Synthetic Biology 2021Quote: ... and then cell suspensions were added with 20% (w/v) glass beads (0.5 mm) and the cells were grinded with hand-powered electrical motor (VWR). Subsequently ...
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bioRxiv - Microbiology 2021Quote: ... 10 μl of the cell lysates were resolved by standard 10% SDS-PAGE gel and electroblotted onto 0.2 mm nitrocellulose membrane (Biotrace, VWR) using a semi-dry transfer unit (Bio-Rad) ...
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bioRxiv - Biophysics 2021Quote: Cells were seeded at low density (~ 10 000 cells/ml) on 24 × 50 mm coverslips with thickness of #1.5 (VWR). After a settling time to allow cells to adhere ...
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bioRxiv - Cell Biology 2021Quote: ... Japanese Collection of Research Bioresources Cell Bank) were cultured in Dulbecco’s Modified Eagle Medium (DMEM) supplemented with 10% fetal bovine serum (FBS, VWR) and an antibiotic mixture (penicillin/streptomycin (PenStrep) ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were lysed by placing the plates on a shaker after adding 250 μL 0.1 M NaOH (VWR, US). Aliquots of 150 μL were pipetted out of each well into a scintillation bottle (Perkin Elmer ...
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bioRxiv - Neuroscience 2022Quote: ... the cell strainer was removed from the testing arena and dropped into a 6-well dish (VWR 10861-554) containing 4% PFA in 1x PBT (1x PBS + 0.25% TritonX100) ...
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bioRxiv - Microbiology 2020Quote: ... and MD40HG cultures in 0.2% FLA-YM (as above) and a no cell negative control were filtered through 0.2 μm cellulose acetate membrane syringe filter (VWR). The filtrates were flash frozen and stored at −80°C until analysis ...
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bioRxiv - Neuroscience 2021Quote: ... supplemented with 1% penicillin-streptomycin and 1% FBS at a density of 250,000 cells per well in 24 well plates (Nunclon) on coverslips precoated with poly-d-lysine and Matrigel (VWR) diluted 1:200 in Minimum Essential Media (HyClone) ...
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bioRxiv - Microbiology 2022Quote: ... Each transformation consisted of 40μL of competent cells and 10ng of the pFAB5516 plasmid library transferred to a chilled cuvette (1mm gap, VWR). Cuvettes were electroporated using a BTX™-Harvard Apparatus ECM™ 630 Exponential Decay Wave Electroporator with the following parameters ...
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bioRxiv - Genomics 2019Quote: Cells were harvested and total genomic DNA was isolated using QuickExtract DNA Extraction Solution (VWR, Radnor, PA, cat# QE09050). PCR was prepared using 5X GoTaq Green Reaction Buffer and GoTaq DNA Polymerase (Promega ...
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bioRxiv - Immunology 2021Quote: ... cell pellets were homogenized in approximately 200 μL of PBS using pre-filled silica bead tubes (VWR, Radnor, Pennsylvania) and a Bead Ruptor Elite homogenizer (OMNI International ...
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The evolution of red colour vision is linked to coordinated rhodopsin tuning in lycaenid butterfliesbioRxiv - Evolutionary Biology 2020Quote: ... cells were seeded at a density of 1.0×106 cells on day 0 in fifteen tissue culture dishes (10 cm diameter, ref 25382-166, VWR) in DMEM High Glucose ...
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bioRxiv - Developmental Biology 2021Quote: ... Cells were further dissociated with 3ml TrypLE Express, washed with Wash media (DMEM, 0.1% BSA) + 1mg/ml DNase (VWR) and centrifuged at 300 rcf for 3 mins ...
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bioRxiv - Cancer Biology 2020Quote: ... through injection of 5×105 67NR murine luminal breast cancer cells in 100 μL of serum-free RPMI (VWR). 67NR cells are locally invasive but non-metastatic ...
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bioRxiv - Cell Biology 2020Quote: Cells were washed twice with ice-cold PBS containing 50 μg/mL of cycloheximide (CHX) (VWR, 94271, Radnor, PA) for 3 minutes ...
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bioRxiv - Immunology 2021Quote: ... washed with AIMV medium without FCS and settled at a number of 2.5×104 cells per well into a 96-well black plate with clear bottom (#734-2480, VWR) for around 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... 2.5 × 104 target cells per well were pipetted into a 96-well black plate with clear-bottom (#7342480, VWR). After a short rest (15 min ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Cells were transfected with hTRPC6 (cloned into pcDNA3) or rat TRPV1 using jetPRIME ® transfection reagent (VWR, Lutterworth, UK). Cells were assayed 48 hours after transfection ...
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bioRxiv - Cancer Biology 2022Quote: ... HT-1080 cells were purchased from ATCC and cultured with DMEM supplemented with 10% fetal bovine serum and 0.1% Gentamycin (VWR). SUM149 (SUM149PT ...
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bioRxiv - Immunology 2022Quote: ... Cells were maintained in Dulbecco’s modified Eagle medium (DMEM) supplemented with 5% (vol/vol) fetal bovine serum (FBS, VWR) and 1% penicillin–streptomycin–glutamine (PSG ...
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bioRxiv - Molecular Biology 2022Quote: ... 5 million cells were seeded on a 15cm plate containing one 25 mm plastic coverslip (Thermanox, VWR 100500-878). At 24 hours after seeding ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were typically grown in 6-well plates after placing round coverslips in the bottom of the wells (VWR), or in PhenoPlates 96-well (Perkin Elmer) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... HEK293T cells were seeded at a density of 0.6 x 106 cells on day 0 in 60 mm culture dishes (ref 25382-100, VWR) in DMEM High Glucose ...
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bioRxiv - Cell Biology 2024Quote: ... cells were washed in PBS and fixed/stained in crystal violet/methanol solution (25% (v/v) methanol (VWR #20847.307) + 5% (w/v ...
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bioRxiv - Developmental Biology 2024Quote: ... which were adjusted to a concentration of 1.25 × 106 cells/mL in iPS Brew + 10 µM ROCK inhibitor (Y-27632, VWR). 800 µL of cell suspension (approximately 1 million cells ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were tested for mycoplasma every 3 months using the Genlantis MycoScope PCR Detection Kit (VWR, 10497-508) and were confirmed to be free of mycoplasma contamination ...
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bioRxiv - Cancer Biology 2023Quote: ... Ramos cells were washed twice and 8.0 x 105 were aliquoted into thirteen 12×75 mm glass reagent tubes (VWR) for each test antibody ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed once with warm PBS and changed to a medium of DMEM without glucose (VWR, 97060-876), 10% FBS ...
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bioRxiv - Bioengineering 2023Quote: The cell culture plate inserts (transwells) for 24 wells (PET membrane, 3.0 µm pore size) were purchased from VWR International.
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bioRxiv - Immunology 2023Quote: ... a total of 107 cells were resuspended in 80μl PBS buffer supplemented with 0.5% BSA (0332, VWR Life Science) and 2mM EDTA (E57040 ...
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bioRxiv - Developmental Biology 2023Quote: ... we used a brush to release the eggs from the agar and transferred them to a cell strainer with 100µm nylon mesh (VWR). To remove the chorion ...
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bioRxiv - Immunology 2024Quote: ... The solution was filtered into a new Eppendorf tube through a 100 µM cell strainer (Falcon/VWR #21008-949). The epithelial cells were then spun down at 1000 g for 5 minutes ...
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bioRxiv - Biophysics 2020Quote: ... The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: ... Briefly, HEK293T cells (ATCC catalog #CRL-3216, RRID:CVCL_0063) were grown in DMEM supplemented with 10% FBS (VWR, catalog #97068-085) and 1% penicillin-streptomycin ...
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bioRxiv - Genomics 2020Quote: ... Cells expressing Cas9 were selected either by sorting for GFP10 or selection with 350 ng/ml Puromycin dihydrochloride (VWR J593) for three consecutive days starting one day after electroporation17 ...
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bioRxiv - Cell Biology 2020Quote: ... The cleared cell lysate was incubated in a lysis buffer-equilibrated Ni-NTA column (HisPur Ni-NTA Superflow Agarose, Pierce, VWR) for 2 h at 4 °C on a rotator ...
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bioRxiv - Neuroscience 2020Quote: ... penicillin/streptomycin and amphotericin B (1X Anti/Anti, #15240062, TFS) (referred to as ‘SHM-10’) on uncoated cell culture flasks (VWR). Cultures were maintained in a sterile incubator (MCO-20AIC ...
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bioRxiv - Cell Biology 2021Quote: ... then lysed cells in 1mL of ice-cold nuclear isolation buffer in a 2mL Dounce homogenizer (VWR, cat. #62400-595). We next filtered the homogenate through a 40µm Flowmi cell strainer (WVR ...
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bioRxiv - Biochemistry 2021Quote: For transductions VeroE6 or HEK cells were seeded into greiner CELLSTAR® 96-well plates or 6-well plates (VWR) containing lentivirus in DMEM containing 2 % FBS and 1 μg/mL polybrene ...
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bioRxiv - Microbiology 2020Quote: ... Cell debris and unbroken cells were removed by centrifugation and supernatants were passed through a 0.22 μm filter unit (VWR International). Soluble protein suspension was loaded onto the Bio-Scale Mini Profinity IMAC Cartridge (5 mL ...
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bioRxiv - Cell Biology 2021Quote: ... 20 μl of cells were put to 180 μl of TYI-S-33 media in 96-wells Tissue Culture Plate (VWR) and the same dilution step was performed 5 times ...
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bioRxiv - Immunology 2022Quote: ... We washed the plate with PBST (Applied Cells, Inc.) five times and added 100 μl 5% BSA (VWR Life Sciences) into each well and incubated at 37°C for 90 minutes ...
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bioRxiv - Neuroscience 2020Quote: ... Human neuronal cells SH-SY5Y (ATCC, Manassas, VA, #CRL-2266) were cultured in DMEM medium (VWR, Radnor, PA, #0101-0500) supplemented with 10% FBS (Gemini Biosciences West Sacramento ...