Labshake search
Citations for Zymo Research :
6051 - 6100 of 6691 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2023Quote: ... microdissected material of around 80 gynoecia (sections of around 24 paraffin blocks with 3 or 4 embedded inflorescences) was pooled in a single tube and RNA extraction was performed with Direct-zol RNA MicroPrep kit (Zymo Research). The integrity and concentration of extracted RNA of each sample was measured using a NanoDrop 2000 (ThermoFisher Scientific) ...
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bioRxiv - Genetics 2023Quote: Total RNA was extracted from frozen tissues using Direct-zol RNA Miniprep plus kit following the manufacturer’s protocol (Zymo Research - R2073). The purity and quantity of the extracted RNA were analyzed using RNA 6000 Nano LabChip Kit and Bioanalyzer 2100 (Agilent CA ...
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bioRxiv - Molecular Biology 2023Quote: Total genomic DNA was extracted from ∼0.2 mg (i) spinach and (ii) mixed-species Teklad samples using a Zymo Quick-DNA Fecal/Soil Microbe Miniprep Kit (Zymo Research). Following extractions ...
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bioRxiv - Molecular Biology 2023Quote: ... Libraries were then size selected by gel purifying DNA on a 10% TBE gel and cleanup was performed using Zymo DNA Cleanup kit (Zymo # D4014) and the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: Total RNA was isolated from the received fungal pellet using conventional TRIzol method followed by column purification using Quick RNA Plant MiniPrep Kit (Zymo Research). The qualities and quantities of the isolated RNA were checked on NanoDrop followed by Agilent TapeStation using High Sensitivity RNA ScreenTape.
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bioRxiv - Molecular Biology 2023Quote: ... RNA from each fraction was first precipitated in EtOH/C2H3NaO2 and then purified through RNA Clean & Concentrator-5 kit following the manufacturer’s protocol (Zymo Reaserach, R1016). RNA distribution profiles were analyzed by qRT-PCR.
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bioRxiv - Neuroscience 2023Quote: ... Total RNA was isolated using the trizol method as described by the manufacturer’s protocol using the Directzol RNA isolation kit (Zymo Research, Germany). The RNA concentration was determined by UV measurement ...
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bioRxiv - Genomics 2023Quote: ... All colonies from each reaction were scraped from the plate and the CROP-seq-opti-Puro-T2A-GFP-sgRNA plasmid library was extracted using ZymoPURE II Plasmid Midiprep Kit (Zymo, D4200). The lentiviral library was generated as stated above with extended virus production time ...
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bioRxiv - Microbiology 2023Quote: ... DNA and RNA were coextracted from filters at Colorado State University using ZymoBIOMICS DNA/RNA Miniprep Kit (Zymo Research Cat. # R2002) coupled with RNA Clean & Concentrator-5 (Zymo Research Cat ...
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bioRxiv - Immunology 2023Quote: RNA was isolated from homogenized colon tissue (fresh, stored in RNAlater or Trizol) or isolated crypts using the Quick-RNA Miniprep Kit (R1055, Zymo Research), or for samples in Trizol ...
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bioRxiv - Neuroscience 2022Quote: RNAlater-preserved samples were homogenized in PBS and total RNA was extracted from samples using the Quick-RNA Miniprep Kit (Zymo Research). RNA extracts were evaluated for concentration and purity using a Nanodrop 8000 instrument (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2022Quote: ... At the indicated time points we harvested cells and extracted RNA using the Direct-zol-96 RNA kit (Zymo #11-331H). Samples were then sent to Novogene for bulk RNA-seq.
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bioRxiv - Bioengineering 2022Quote: cDNA samples were again purified via column purification using a Zymo Research DNA Clean and Concentrator-25 kit (Zymo, Irvine, CA). Select 200-300 bp regions were then amplified using specific primers designed against various hotspot regions found in the patient-sample derived consensus sequence (Supplementary Table 5) ...
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bioRxiv - Neuroscience 2023Quote: RNA was extracted from neocortical regions of slice cultures using Trizol reagent followed by RNA Clean & Concentrator kit (Zymo research R1014). cDNA was synthesized with 0.5 μg of RNA using the cDNA Synthesis Kit (Bio-Rad 170-8891 ...
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bioRxiv - Systems Biology 2023Quote: ... to exclude small fragments (less than about 200bp) and the DNA was eluted from the gel slices using the Zymoclean Gel DNA Recovery Kit (Zymo, #D4001), followed by quantification (Qubit ...
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bioRxiv - Cell Biology 2023Quote: Total RNA was extracted from 2-5 x107 cells grown in different nutrients (see above) with the YeaStar RNA Kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... before being spun down (10k rpm, 2 minutes) and resuspended in 80 μL of digestion buffer (included in the YeaStar RNA Kit, Zymo Research). Cells were then stored on ice until ready for RNA extraction ...
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bioRxiv - Immunology 2023Quote: ... DNA obtained from anti-OVA hits were then cut out from agarose gels and amplicons from heavy and light chains were purified separately by gel extraction (Zymoclean Gel DNA Recovery Kit, Zymo Research).
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bioRxiv - Genomics 2023Quote: ... Tissue was then homogenized with steel beads and total RNA was extracted using the Direct-zol™ RNA MicroPrep kit from Zymo Research ...
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bioRxiv - Genetics 2023Quote: ... and proteinase K (2 h at 65°C) and DNA was extracted with the ChIP DNA Clean & Concentrator kit (Zymo Research). Libraries for sequencing were prepared with a modified version of the Next Ultra II DNA Library Prep Kit for Illumina (NEB ...
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bioRxiv - Developmental Biology 2023Quote: ... The genomic DNA was treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit following the protocol of the manufacturer (Zymo Research). DNA was amplified by 30 cycles of a three-step PCR (98℃ for 10 sec ...
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bioRxiv - Evolutionary Biology 2022Quote: RNA was extracted from 30 μL of each virus inoculum using a Direct-zol RNA Miniprep kit (Zymo Research, Irvine, CA) following the manufacturer’s protocol ...
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bioRxiv - Genomics 2023Quote: ... 350 ng of each extracted sperm DNA and 350 ng of control human NA12878 lymphoblastoid cell line genomic DNA (Coriell Institute) were bisulfite-converted using the Zymo EZ DNA Methylation kit (Zymo Research). The loci were amplified by PCR using the following primer sets ...
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Striated muscle-specific base editing enables correction of mutations causing dilated cardiomyopathybioRxiv - Genetics 2022Quote: ... was added to 200 μl of homogenized tissue (see DNA extraction) and processed using the Direct-zol RNA Miniprep Kit (Zymo Research) with on-column DnaseI digestion ...
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bioRxiv - Systems Biology 2023Quote: ... We ran this concentrated library on a 2% agarose 1X TAE gel with an EtBr stain at 90 V for 85 minutes and extracted the resulting 636 bp band using a DNA Clean & Concentrator-5 Kit (Zymo, #D4014) with a 20 μL MB H2O elution ...
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bioRxiv - Biochemistry 2023Quote: ... Plasmids used for transient transfections in HEK293T cells were prepared and purified using the Zymopure Midiprep Kit (Zymo Research, cat #D4201). Mutations to create the CFTR ΔPRF constructs were chosen using RNA structure predictions performed by the ViennaRNA RNAfold Web Server.
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bioRxiv - Molecular Biology 2023Quote: RNA for RNA-seq was obtained by processing cerebellar tissue or CGN cultures with Trizol reagent followed by clean up on the Zymo Direct-zol RNA miniprep kit (Zymo #R2052). RNA purity was measured to ensure samples had A260/280 and A260/230 values > 1.9 ...
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Disruption of the Aspergillus fumigatus RNA interference machinery alters the conidial transcriptomebioRxiv - Microbiology 2023Quote: ... The reaction was ended with EDTA for 5 min at 65°C followed by cleanup using the RNA Clean and Concentrator-25 kit (Zymo Research) as described above ...
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bioRxiv - Cell Biology 2023Quote: ... Bacteria containing the constructs were then grown overnight at 37°C and the DNA was extracted using the Zyppy Plasmid Miniprep Kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Plant Biology 2022Quote: ... the DNA fragments were treated twice with bisulfite using an EZ DNA Methylation-Gold™ Kit (Zymo Research, Orange, CA, USA). The libraries were then prepared according to the Illumina standard DNA methylation analysis protocol.
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bioRxiv - Genetics 2023Quote: ... Five hundred nanograms of gDNA was bisulfite converted following manufacturer’s specifications using the EZ DNA Methylations kit (Zymo Research; Irvine, CA). Bisulfite-treated gDNA was amplified from −660 to +97 from the transcription start site of Pl1-Rhoades (Cone et al. ...
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bioRxiv - Neuroscience 2023Quote: ... 88∼135 bp) were resolved on a 10% TBE polyacrylamide gel and extracted separately using the DNA Clean & Concentrator-5 kit (Zymo Research).
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bioRxiv - Neuroscience 2023Quote: ... RNA was isolated from temporal cerebral cortex samples using the Zymo Quick-DNA/RNA Miniprep Plus Kit (Zymo, Irvine, CA, USA) and purified using the RNA Clean and Concentrator-5 kit (Zymo) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Yeast transformations (plasmids and linear fragments for genomic integration) were done using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). All other chemicals were obtained from Merck unless otherwise specified.
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bioRxiv - Biophysics 2024Quote: ... This was done by isolating genomic DNA of each yeast strain using the Zymoprep yeast plasmid miniprep II kit (Zymo Research) according to the manufacturer’s protocol and PCR amplifying the region of interest.
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bioRxiv - Synthetic Biology 2024Quote: ... colonies were scraped with 50 mL 2XYT and plasmid DNA extracted with the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research, D4203). These plasmid libraries constitute the “post-selection libraries.”
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bioRxiv - Cell Biology 2024Quote: ... Assembled plasmids (termed pTREP-ID vector hereafter) were then purified using a DNA Clean and Concentrator purification kit (Zymo, no. D4014) and transformed into One Shot ccdB Survival 2 T1R resistant competent cells (Thermo Fisher ...
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bioRxiv - Synthetic Biology 2024Quote: ... coli were grown overnight in LB with the appropriate selective antibiotic and DNA was prepped using a ZymoPURE Plasmid Miniprep Kit (Zymo #D4210) by following the manufacturer’s instructions.
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bioRxiv - Synthetic Biology 2024Quote: ... Donor DNA was generated by PCR amplification from the pre-assembled donor DNA plasmids and purified by DNA Clean & Concentrator Kit (Zymo Research).
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed donor DNA fragment and recombinant plasmid pYlCas9 were simultaneously transformed into the targeted strain using the Frozen-EZ yeast transformation II Kit (Zymo Research). The amounts of donor DNA and plasmid pYlCas9 used were 1 μg and 500 ng ...
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bioRxiv - Plant Biology 2024Quote: ... total RNA was extracted from approximately 50 mg of finely minced tissue in all cases using the Quick-RNA™ Plant Miniprep kit from ZYMO RESEARCH® (ref ...
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bioRxiv - Neuroscience 2023Quote: ... and RNA was extracted with the phenol-chloroform-based procedure or using Direct-zol RNA Miniprep Plus kit (Zymo research, R2070) following the manufacturer’s protocols ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants for each sample were carefully transferred to RNase-free tubes for purification by the Direct-Zol RNA Miniprep Plus RNA purification kit (Zymo Research), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: RNA was purified and concentrated according to the protocol for RNA Clean and Concentrator Kit - 5 (Zymo Research Corporation, Irvine, CA) with an eluted volume between 30-35µL ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from cultured cells or myocardial tissue of mice euthanized at the timepoints reported above using the Direct-zol RNA purification Kit (ZYMO Research) following the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2023Quote: ... nucleus accumbens and lung of the 4 juvenile and 4 old female mice was bisulfite converted using the EZ DNA methylation Gold kit (Cat# D5006, Zymo Research) according to manufacturer’s instructions and eluted ...
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bioRxiv - Biochemistry 2023Quote: ... All plasmids used for transient transfection experiments were purified with an endotoxin removal step (ZymoPURE Plasmid Miniprep Kit from Zymo Research). The PEmax** protein expression vector (pET-21a-PEmax**-6His ...
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bioRxiv - Bioengineering 2023Quote: ... and the correct size band was cut out and extracted from the agarose with the Zymo Gel recovery kit (Zymo D4002). We observe that libraries that contained left probes containing the non-read 2 priming regions produced some nonspecific amplification requiring size selection purification ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR product was run on a 2% Agarose gel and the band at 150 bp was excised and then purified using the Zymoclean Gel DNA Recovery kit (Zymo Research). The dgRNA was synthesized via IVT using the MEGAscript T7 Transcription kit supplemented with 10mM ATP ...
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bioRxiv - Genomics 2023Quote: ... was transformed with 2 uL of each ligation reaction and resulting colonies were selected for plasmid DNA isolation using the ZymoPure Plasmid miniprep kit (Zymo Research). Each cloned gRNA sequence-containing PB-CRISPRia plasmid was verified by Sanger sequencing (Quintara Bio).