Labshake search
Citations for Zymo Research :
5851 - 5900 of 6691 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2022Quote: ... pombe DNA was extracted from cells grown to log phase at 32°C using a YeaStar genomic DNA purification kit (Zymo Research). Equal quantities of DNA from four spontaneous canavanine-resistant clones derived from strain 2299 were combined ...
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bioRxiv - Developmental Biology 2022Quote: ... Glycerol stocks of the plasmids that were confirmed correct by Sanger sequencing were grown and prepared for maxiprep plasmid isolation (Zyppy Plasmid Maxiprep Kit, Zymo 6431). Proper cloning was achieved for all gRNAs.
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bioRxiv - Evolutionary Biology 2022Quote: ... The NEBNext® Ultra™ II DNA Library Prep Kit for Illumina was used for library preparation and was combined with EZ-96 DNA Methylation-Gold™ MagPrep (ZYMO) for bisulfite libraries ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Prepared lysates were stored at -80°C until RNA extraction was performed using the Quick-RNA MiniPrep kit (Zymo Research #R1054), following the manufacturer’s instructions.
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bioRxiv - Genomics 2022Quote: ... DNA was extracted from the brain and muscle tissue separately using a Quick-DNA™ MagBead Plus kit (Zymo Research, 2017) and quantified using a Qubit™ dsDNA HS Assay Kit on a Qubit™ 3 Fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... The supernatants were collected and the total RNA was then extracted using the Direct-zol RNA MiniPrep Kit (R2052, Zymo Research) and quantified using NanoDrop 2000 ...
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bioRxiv - Evolutionary Biology 2022Quote: ... DNA was first extracted from pools (10 flies for each cage and time point) using the Quick-DNA Plus kit (Zymo D4068), which includes a proteinase K digestion to ensure unbiased sampling of diverse bacteria ...
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bioRxiv - Plant Biology 2022Quote: ... YES3-Gate-OPT3 construct or YES3-Gate lacking the cDNA insert were transformed into appropriate yeast line using the Frozen-EZ Yeast Transformation II kit (Zymo Research). Transformants were selected for uracil prototrophy on YNB medium containing 6.7% (w/v ...
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bioRxiv - Cell Biology 2022Quote: ... The samples were then placed on a magnet and the immunoprecipitated DNA samples in the supernatant along with the input samples were purified using ChIP Purification Kit (Zymo Research), according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2022Quote: ... DNA was purified from proteinase K treated samples using a DNA purification kit following the manufacturer instructions (Zymo Research, CA, USA) and was subsequently analyzed either by running a 2% low melting agarose (APEX ...
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bioRxiv - Microbiology 2022Quote: ... DNA and RNA were extracted using the Quick DNA/RNA mini prep plus kit with ZR BashingBeads (0.1 and 0.5mm; Zymo Research, Carlsbad, CA). For coral samples ...
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bioRxiv - Cancer Biology 2022Quote: ... total RNA from biological triplicates of HMLE-Twist and HMLE-pBp cells was extracted (12 biological samples in total) with Quick-RNA Miniprep kit (Zymo Research) according to manufacturer protocol ...
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bioRxiv - Physiology 2022Quote: ... gastrocnemius muscle RNA from Sham and SMC mice were isolated with the Direct-zol RNA Miniprep Plus kit (Zymo Cat#: R2070). RNA library construction and sequencing were performed by the High-Throughput Genomics Core at the Huntsman Cancer Institute ...
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bioRxiv - Microbiology 2019Quote: ... the 180 basepair-long amplicons were purified by using a Zymo Research DNA Clean and Concentrator™ Kit according to the manufacturer’s protocol (Zymo Research) and eluted in in 30 μl 1× Tris-EDTA (TE ...
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bioRxiv - Microbiology 2019Quote: ... and PCR products were extracted and purified from agarose gels using the Zymoclean Gel DNA Recovery Kit (Zymo Research, Irvine, CA). The Zyppy Plasmid Miniprep Kit (Zymo Research ...
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bioRxiv - Microbiology 2019Quote: ... Digests were heat inactivated for 20 minutes at 80°C then column cleaned using the DNA Clean and Concentrator kit (Zymo Research). Dilute ligations (100-500 ng DNA ...
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bioRxiv - Developmental Biology 2019Quote: Sodium bisulfite modification of genomic DNA (500 ng) of WT and Etv5 KO mESCs was performed according to the instruction of EZ DNA Methylation-DirectTM Kit (Zymo Research). Methylation specific PCR for a fragment of Fgfr2 promoter was carried out as we previously described (Zhang et al. ...
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bioRxiv - Molecular Biology 2019Quote: ... 100 µL of this soma sample was then carried forward for RNA isolation using the Zymo QuickRNA MicroPrep kit (Zymo R1050). In parallel ...
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bioRxiv - Molecular Biology 2019Quote: ... 50 ng of genomic DNA was digested with the appropriate restriction endonuclease and purified using Genomic DNA Clean & Concentrator kit (D4065-Zymo Research). 0.6 ng was used as template for qPCR analysis using PowerUp SYBR Green Master Mix (Life Technologies ...
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bioRxiv - Molecular Biology 2019Quote: ... Two rounds of bisulfite conversion of the adapter-ligated DNA were performed using EZ-DNA methylation Gold Kit (D5005-Zymo Research), and used as template for three separate PCR reactions (eight cycles of amplification ...
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bioRxiv - Bioengineering 2019Quote: The frozen samples were subjected directly to DNA extraction with the ZymoBIOMICS™ DNA Miniprep Kit (Zymo Research, Irvine, CA, USA), using a PowerLyzer® 24 Bench Top Bead-Based Homogenizer (MO BIO Laboratories ...
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bioRxiv - Microbiology 2019Quote: DNA fragments < 700 bp in length were isolated from 9 μg of total genomic DNA using a Select-a-Size DNA Clean & Concentrator kit (Zymo Research) according to manufacturer’s recommendations ...
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bioRxiv - Microbiology 2019Quote: Sixteen sputa collected from nine Australian adult CF patients underwent DNA extractions using the Zymo Quick-DNA Miniprep Plus Kit (ZYMO Research) protocol ...
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bioRxiv - Synthetic Biology 2019Quote: ... the 6633 basepair-long amplicons were purified by using a Zymo Research DNA Clean and Concentrator™ Kit according to the manufacturer’s protocol (Zymo Research) and eluted in 30 µl 0.5× Tris-EDTA (TE ...
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bioRxiv - Plant Biology 2019Quote: ... The PCR products were analyzed via agarose gel electrophoresis and the clean-up was either done by bead purification with HighPrepTM PCR (MAGBIO) or column purification with the DNA Clean & ConcentratorTM kit (Zymo Research). The purified samples were send for Sanger sequencing (Eurofins Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... RNA was released by proteinase K treatment in urea and recovered by phenol chloroform extraction and column purification (RNA Clean-Up kit; Zymo Research). Input samples were dephosphorylated and 3’-linker ligated to a custom oligonucleotide primer and all samples (IPs and SMInputs ...
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bioRxiv - Molecular Biology 2019Quote: ... the DNA was treated with sodium bisulfite under non-denaturing conditions (no denaturation and incubation at 37°C for 3 hrs) using the Zymo Lightning bisulfite modification kit (Zymo Research). Following sample clean-up ...
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bioRxiv - Biochemistry 2019Quote: ... the DNA samples used for 450k analysis were digested overnight at 37°C using BamHI and EcoRI and then bisulfite converted using EZ DNA Methylation-Lightning Kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Genetics 2019Quote: ... genomic DNA was isolated from a small aliquot of mixed mouse and fly cells using Quick-DNA Miniprep kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2020Quote: ... and Spag17Pcdo/Pcdo animals were purified and concentrated using the Zymo DNA clean and Concentrator kit (Zymo Research Corporation, Irvine, CA). 100ng of concentrated DNA were used in each Sanger sequencing reaction.
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bioRxiv - Molecular Biology 2020Quote: ... 800 ng of DNA from each participant was bisulphite treated using the EZ Zymo Methylation Kit (D5002, Zymo Research, CA, USA) following the alternative over-night incubation conditions for use prior to the Illumina® Infinium MethylationEPIC Kit provided in the published protocol ...
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bioRxiv - Molecular Biology 2020Quote: ... The gel slice corresponding to 70-90 nucleotides was excised from the PAGE gel and the RNA fragments were recovered using ZR small-RNA PAGE Recovery Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Plant Biology 2020Quote: ... Amplicons were excised from a 2 % agarose gel (200-800 bp fragments were excised) and purified using the Gel DNA recovery kit (Zymo Research). Library concentrations were measured with qPCR according to Illumina’s Sequencing Library qPCR Quantification Guide ...
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bioRxiv - Pathology 2020Quote: ... the cells were washed with PBS three times and RNA was extracted using an RNA extraction kit (Zymo, Irvine, CA, USA). In a parallel experiment ...
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bioRxiv - Synthetic Biology 2021Quote: ... After the second treatment with DNase I the samples were column purified and concentrated using RNA Clean & Concentrator-5 kit (Zymo Research). Quality of RNA in each sample was checked on 1% agarose gel and its concentration measured on a Qubit 4 fluorometer (Invitrogen) ...
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bioRxiv - Genomics 2020Quote: ... 4-SUTP containing RNA was eluted in 100 mM DTT buffer and purified with RNA Clean and Purification kit (Zymo Research) with in-column DNAseq reaction to eliminate traces of genomic DNA ...
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bioRxiv - Molecular Biology 2021Quote: Total RNA from three-week-old or four-week-old plant leaves was isolated from Arabidopsis plants and from in vitro potato cultures using a Direct-zol RNA MiniPrep Kit (Zymo Research). A TRIzol™ reagent (Invitrogen)-based protocol was used for the reverse transcription reaction performed with Superscript™ III Reverse Transcriptase (Invitrogen) ...
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bioRxiv - Microbiology 2021Quote: ... RNA extractions were carried out using a modified version of the Quick-RNA™ MiniPrep kit (Zymo Research, Irvine, CA, USA), as follows ...
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bioRxiv - Microbiology 2021Quote: ... as well as two replicates of sorted yeast display library based on RBD-binding and expression were extracted from sorted yeast cells using a Zymoprep Yeast Plasmid Miniprep II Kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2021Quote: Bacterial DNA extracted from individual particle-attached communities was quantified through qPCR of the 16S rRNA gene using the Femto Bacterial DNA Quantification Kit (Zymo Research), which has a lower limit of detection of 20fg ...
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bioRxiv - Synthetic Biology 2020Quote: ... Mycelium was disrupted with a FastPrep FP120 system (Qbiogene) and total RNA was isolated using the Direct-zol RNA MiniPrep Kit (Zymo Research). For cDNA synthesis ...
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bioRxiv - Plant Biology 2020Quote: ... and total RNA was extracted from the tissue powder using a Direct-Zol RNA MiniPrep kit with DNase I treatment (Zymo Research) according to the manufacturer’s instruction ...
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bioRxiv - Genomics 2021Quote: ... The nascent RNA was purified by adding 3 volumes of TRIzol and processed with the Direct-zol RNA Miniprep kit (Zymo Research) with addition of 1/100 volume of 100 mM DTT to each supplied wash buffer.
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bioRxiv - Genomics 2021Quote: ... The PCR products were run on an UltraPure Agarose (Thermo Fischer Scientific) gel and cDNA fragments larger than 500 nt were purified using the Zymoclean Large Fragment DNA Recovery Kit (Zymo Research). The library was prepared using the ONT 1D kit (SQK-LSK108 ...
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bioRxiv - Plant Biology 2020Quote: ... total RNA were prepared from rosettes of three biological replicates of four (Fig. 10) and five (Fig. 6) week-old plants using the Direct-zolTM RNA Mini Prep Kit (Zymo Research). DNA contaminations were removed using Turbo DNase I ...
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bioRxiv - Immunology 2020Quote: ... plasmid DNA was isolated from 10 million yeast from each round using a Zymoprep Yeast Miniprep Kit (Zymo Research; Irvine, CA). Amplicons were generated to capture the peptide through the 3C protease site ...
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bioRxiv - Immunology 2020Quote: ... Cloning and sequencing of the full ORF cDNA of AgPGE2R was performed from total RNA isolated from hemocytes perfused from non-blood fed female mosquitoes (n=50) using Direct-zol RNA Miniprep kit (Zymo research). After DNase I treatment following manufacture’s protocol (New England Biolabs) ...
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bioRxiv - Evolutionary Biology 2019Quote: DNA was extracted from pools of approximately 15,000 to 20,000 surviving larvae from each replicate vessel using a Zymo ZR-Duet DNA/RNA MiniPrep Plus Kit (Zymo, Irvine, CA). DNA was shipped to Rapid Genomics (Gainesville ...
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bioRxiv - Evolutionary Biology 2019Quote: A total RNA sample enriched for small RNA was extracted from 500 pooled individuals using the ZR RNA MicroPrep kit (Zymo Research). miRNA library construction service from total RNA using Illumina TrueSeq Small RNA kit and sequencing on a NextSeq 500 Mid instrument was outsourced to StarSEQ GmbH ...
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bioRxiv - Plant Biology 2019Quote: ... frozen in liquid nitrogen and then RNA was extracted using the Direct-Zol Plant RNA extraction kit according to the manufacturer’s instruction (Zymo Research, Germany). Quality of the RNA was assessed using gel electrophoresis and quantity using a NanoDrop ...