Labshake search
Citations for Zymo Research :
6101 - 6150 of 6691 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genetics 2023Quote: ... Five hundred nanograms of gDNA was bisulfite converted following manufacturer’s specifications using the EZ DNA Methylations kit (Zymo Research; Irvine, CA). Bisulfite-treated gDNA was amplified from −660 to +97 from the transcription start site of Pl1-Rhoades (Cone et al. ...
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bioRxiv - Genomics 2023Quote: ... was transformed with 2 uL of each ligation reaction and resulting colonies were selected for plasmid DNA isolation using the ZymoPure Plasmid miniprep kit (Zymo Research). Each cloned gRNA sequence-containing PB-CRISPRia plasmid was verified by Sanger sequencing (Quintara Bio).
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bioRxiv - Cancer Biology 2023Quote: ... change of reverse transcription primers for compatibility with standard Illumina sequencing primers 3) cDNA clean-up prior to library amplification performed with the DNA Clean & Concentrator-5 kit (Zymo, #D4013) 4 ...
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bioRxiv - Bioengineering 2023Quote: ... The PCR product was run on a 2% Agarose gel and the band at 150 bp was excised and then purified using the Zymoclean Gel DNA Recovery kit (Zymo Research). The dgRNA was synthesized via IVT using the MEGAscript T7 Transcription kit supplemented with 10mM ATP ...
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bioRxiv - Molecular Biology 2023Quote: ... gel fragments corresponding to 190 – 350 nts in DNA size were excised and libraries extracted using the Zymoclean Gel DNA Recovery Kit (D4007, Zymo Research). DNA concentrations were measured using the Qubit dsDNA HS Assay Kit (Q32854 ...
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bioRxiv - Microbiology 2023Quote: Isolated poly(A)+ RNA was firstly subjected to a clean-up and concentration step using the RNA clean & concentrator kit (Zymo Research). Then ...
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bioRxiv - Molecular Biology 2023Quote: ... nucleus accumbens and lung of the 4 juvenile and 4 old female mice was bisulfite converted using the EZ DNA methylation Gold kit (Cat# D5006, Zymo Research) according to manufacturer’s instructions and eluted ...
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bioRxiv - Neuroscience 2023Quote: ... and RNA was extracted with the phenol-chloroform-based procedure or using Direct-zol RNA Miniprep Plus kit (Zymo research, R2070) following the manufacturer’s protocols ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was isolated from temporal cerebral cortex samples using the Zymo Quick-DNA/RNA Miniprep Plus Kit (Zymo, Irvine, CA, USA) and purified using the RNA Clean and Concentrator-5 kit (Zymo) ...
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bioRxiv - Neuroscience 2023Quote: ... 88∼135 bp) were resolved on a 10% TBE polyacrylamide gel and extracted separately using the DNA Clean & Concentrator-5 kit (Zymo Research).
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bioRxiv - Immunology 2023Quote: ... Approximately 50 ng genomic double-stranded DNA per donor was converted with sodium bisulfite using the EZ DNA Methylation-Lightning Kit (Zymo Research) and fragmented by sonication (Covaris S220 ...
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bioRxiv - Microbiology 2023Quote: ... Supernatants for each sample were carefully transferred to RNase-free tubes for purification by the Direct-Zol RNA Miniprep Plus RNA purification kit (Zymo Research), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: RNA was purified and concentrated according to the protocol for RNA Clean and Concentrator Kit - 5 (Zymo Research Corporation, Irvine, CA) with an eluted volume between 30-35µL ...
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bioRxiv - Molecular Biology 2023Quote: Total RNA was extracted from cultured cells or myocardial tissue of mice euthanized at the timepoints reported above using the Direct-zol RNA purification Kit (ZYMO Research) following the manufacturer’s protocol.
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bioRxiv - Cell Biology 2023Quote: Genomic DNA was extracted from 200-250µm-thick cryopreserved rat lung tissue using the Zymo Quick-DNA MicroPrep Kit (Zymo, Irvine, CA), according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: RNA from exponentially growing or senescent human and bowhead whale primary skin fibroblasts was isolated using the Quick-RNA MiniPrep kit (Zymo Research) according to manufacturer instructions.
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bioRxiv - Biochemistry 2023Quote: ... All plasmids used for transient transfection experiments were purified with an endotoxin removal step (ZymoPURE Plasmid Miniprep Kit from Zymo Research). The PEmax** protein expression vector (pET-21a-PEmax**-6His ...
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bioRxiv - Bioengineering 2023Quote: ... and the correct size band was cut out and extracted from the agarose with the Zymo Gel recovery kit (Zymo D4002). We observe that libraries that contained left probes containing the non-read 2 priming regions produced some nonspecific amplification requiring size selection purification ...
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bioRxiv - Evolutionary Biology 2024Quote: Total RNA from the heart and testis of the Madagascar ground gecko was purified using ISOGEN (#311-02501, Nippon Gene) and Direct-zol RNA Miniprep kit (#R2050, Zymo Research). The cDNA was synthesized from total RNA using Verso cDNA synthesis kit (#AB1453A ...
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bioRxiv - Synthetic Biology 2024Quote: RNA was extracted from callus and protoplast cultures using the ZR Plant RNA Miniprep™ kit (R2024, Zymo Research, CA, USA) and TRIzol™ Reagent (15596026 ...
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bioRxiv - Cell Biology 2024Quote: ... RNA from tdTomato positive SRSF2 knockout and P95H mutant cells and reporter positive control cells was extracted using the Direct-zol RNA microprep kit (Zymo Research), following manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Genomic DNA was extracted from 1.5 mL cultures of Bacteroides strains using the Zymobionics DNA Miniprep kit (Zymo Research, Irvine, CA) and used as input for IS-Seq as described previously 19 ...
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bioRxiv - Genomics 2024Quote: ... Total RNA (∼700 ng) was used to make RNA-seq libraries following the Zymo-Seq Ribo-free Total RNA Library Kit (Zymo Research) instructions with dual indexing ...
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bioRxiv - Plant Biology 2024Quote: Preparation of RNA samples from seedlings of the indicated genotypes and growth conditions was performed using a Quick-RNA MiniPrep Kit with on-column DNase I treatment (Zymo Research). cDNA synthesis was performed with 1.5 µg of total RNA using a Superscript IV First-Strand cDNA Synthesis Kit (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from infected cells 72 h post infection using Quick-DNA Miniprep kit (Zymo Research, catalogue no. D3024).
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bioRxiv - Genomics 2024Quote: ... An aliquot of 250 ng DNA from each sample was bisulfite converted using the EZ DNA Methylation kit (Zymo Research, #D5001) according to manufacturer’s instructions for Illumina DNA methylation arrays ...
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bioRxiv - Microbiology 2024Quote: A whole-stranded transcriptome RNA library was generated using the Zymo-Seq RiboFree Total RNA kit (Zymo Research, Irvine, CA, USA), following the manufacturer’s protocol ...
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bioRxiv - Biochemistry 2024Quote: ... contains the Arabidopsis thaliana cytochrome P450 reductase I gene, ATR1) (Urban et al., 1997) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research). For plant transient expression ...
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bioRxiv - Cell Biology 2024Quote: ... 25 µl of RNase free water was added to the mixture and transcribed RNA was eluted using the RNA Clean & Concentrator kit (Zymo Research). Samples were assessed for their length and concentration by Bioanalyzer (Agilent ...
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bioRxiv - Microbiology 2024Quote: DNA was isolated from freshly-grown colonies using the Zymo Quick-DNA Fungal/Bacterial Miniprep Kit (Zymo Research, Irvine, CA, USA). Libraries were prepared using the Nextera DNA Flex Library Prep as recommended by the manufacturer (Illumina ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed twice with PBS and RNA was isolated using Direct-zol RNA miniprep Kit (Zymo research, cat. no. R2050). RNA was quantified and processed for cDNA synthesis using Reverse Transcriptase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... 5 µl of every amplicon from the same RT product was pooled and then purified using a DNA Clean & Concentrator-5 kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2024Quote: ... PCR products for both pairs of primers were pooled and then purified using a DNA Clean & Concentrator-5 kit (Zymo Research) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Total RNA was collected over a time course of 24 hours and isolated using a Quick-DNA/RNA™ Miniprep kit (Zymo) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2024Quote: ... Bacteria expressing the correct plasmid were grown in bulk and high-purity plasmid was extracted using ZymoPURE Plasmid Midiprep kit (Zymo research) and verified by sequencing of the coding region of the influenza virus segment.
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bioRxiv - Molecular Biology 2024Quote: ... Genomic DNA was extracted using the manufacturer’s instructions using a YeaStar Genomic DNA kit (Catalog D2002, Zymo Research, Irvine, California, USA). Genomic DNA was subject to a PCR to check for the insertion of the corresponding tag or the CHC KO.
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bioRxiv - Molecular Biology 2024Quote: ... RNA was extracted from cells expressing the iRS3 reporter 3h post-induction using the Direct-zol™ RNA MiniPrep kit (Zymo). After extraction ...
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bioRxiv - Zoology 2024Quote: ... and post-aestivating (55 days old) female beetles were extracted using the Quick-RNA Tissue/Insect Kit (Zymo Research, Freiburg, Germany). Subsequently ...
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bioRxiv - Developmental Biology 2024Quote: ... DNA around binding sites was cleaved with pAG-MNase enzyme and the released DNA was reverse-crosslinked with proteinase K and 0.1% SDS overnight at 65°C before purification with a ChIP DNA Clean & Concentrator kit (Zymo Research D5205). DNA was converted into Illumina-compatible libraries with the NEBNext(R ...
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bioRxiv - Genetics 2024Quote: ... Two micrograms of DNA were subjected to sodium bisulfite conversion using an EZ DNA Methylation Kit (Zymo Research, Irvine, CA, USA). Genomic regions were subsequently amplified using EpiTaq HS (Takara Bio ...
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bioRxiv - Cell Biology 2024Quote: Yeast transformations were performed either using a standard lithium acetate transformation or Zymo Frozen-EZ Yeast Transformation II Kit (Zymo #T2001) before plating on appropriate selection media82 ...
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bioRxiv - Neuroscience 2024Quote: ... Equal amounts of ethanol were added to each of the samples before eluting the RNA using a Direct-zol RNA kit (Zymo Research) using manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2024Quote: Yeast genomic DNA was extracted from the potential aMF biosensor yeast strains and the unedited yWS677 strain (to be used as negative control in PCR analysis) using the YeaStar Genomic DNA kit and protocol (Zymo Research), with no known deviations.
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bioRxiv - Systems Biology 2022Quote: ... This flow through was discarded and 1 volume of DNA/RNA Prep buffer (Zymo) was added then centrifuged ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Cells were lysed in 300ul of RNA lysis buffer (Zymo Research, #R1060-1-50). Prepared lysates were stored at -80°C until RNA extraction was performed using the Quick-RNA MiniPrep kit (Zymo Research #R1054) ...
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bioRxiv - Immunology 2022Quote: ... Additional tissues were treated using TRIzol reagent (Zymo Research Catalog No R2050-1-200) at a ratio of at least 1:1 or fixed with 10% neutral-buffered formalin before additional work in BSL2 conditions.
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bioRxiv - Developmental Biology 2020Quote: ... 1 μL CircLigase) for 12 hours at 60°C and subsequently purified by Zymo RNA Clean & Concentrator 5 columns (100 μL sample ...
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bioRxiv - Microbiology 2023Quote: ... 1) Amplification of a ZymoBIOMICS Microbial Community DNA Standard (Zymo Research, Irvine, CA U.S.A.) and 2 ...
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bioRxiv - Genomics 2023Quote: Cells were lysed in 300μl of RNA lysis buffer (Zymo Research #R1060-1-50), and stored at -80°C until RNA extraction was performed using the Quick-RNA MiniPrep kit (Zymo Research #R1054 ...
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bioRxiv - Microbiology 2023Quote: ... Samples were immediately stored in 1 ml of DNA/RNA Shield presentation buffer (Zymo) and frozen at -20°C until DNA extraction.