Labshake search
Citations for Zymo Research :
6251 - 6300 of 6691 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: We extracted RNA and DNA from tissue biopsies using the ZR-Duet DNA/RNA MiniPrep Plus kit (Zymo Research, catalog number D7003). We performed the in-column DNase I treatment as specified in the kit to remove residual DNA from the RNA fraction ...
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bioRxiv - Biophysics 2021Quote: ... RNA was then extracted from the aqueous phase using a spin column-based purification kit (RNA Clean & Concentrator-5, Zymo Research # R1015) and reverse-transcribed into cDNA using SuperScript VILO Master Mix (Thermo Fisher # 11755050) ...
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A Data-Driven Transcriptional Taxonomy of Adipogenic Chemicals to Identify White and Brite AdipogensbioRxiv - Pharmacology and Toxicology 2021Quote: ... Total RNA was extracted and genomic DNA was removed using the Direct-zol MagBead RNA Kit and following manufacturer’s protocol (Zymo Research, Orange, CA). RNA concentrations and contamination were determined spectrophotometrically using a Nanodrop (ND-1000 ...
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bioRxiv - Microbiology 2021Quote: Six PCR reactions from each sample were pooled and purified with the DNA Clean & Concentrator™ kit (Zymo Research, Irvine, CA, USA), and quantified with Qubit 1.0 fluorometer (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2019Quote: ... #10296010) were added to the flow-through and RNA was extracted using the Direct-zol RNA Mini-Prep kit (Zymo Research #R2052) as per the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... The RNA was purified and treated with DNase by using the Direct-zol RNA MiniPrep kit (Zymo Research Corporation, Orange, CA, USA), according to the manufacturer’s recommendation ...
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bioRxiv - Microbiology 2021Quote: ... DNA was extracted from 20 mg of each tissue using the ZYMO Quick-DNA™ Miniprep Plus Kit (Zymo Research, Irvine, CA). Resulting DNA was amplified neat and diluted 1:2 ...
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bioRxiv - Immunology 2021Quote: DNA for HLA typing was isolated from PBMCs using the Quick-DNA/RNA™ Microprep Plus Kit (Zymo Research, Irvine, CA, USA). High throughput-HLA typing was performed by the Institute of Transplantation Diagnostics and Cell Therapeutics (ITZ) ...
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bioRxiv - Immunology 2021Quote: A total of 200 ng genomic DNA was subjected to sodium bisulfite treatment and purified using the EZ DNA Methylation-Gold Kit (Zymo research, D5007) according to the manufacturer’s specifications ...
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bioRxiv - Immunology 2021Quote: Viral (v)RNA was extracted from the respiratory secretions of COVID-19 patients using the Quick-RNA™ Viral Kit (Zymo Research) following the manufacturer’s protocol and complementary (c)DNA synthesized using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems™ ...
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bioRxiv - Immunology 2021Quote: ... followed by a final clean-up step by agarose-gel extraction (2% (w/v) gel) with Zymoclean Gel DNA Recovery kit (Zymo Research D4001). Yeast scFv display libraries were generated using the amplified VH and VL DNA libraries from above and the pYD1 yeast surface display vector (Addgene plasmid #73447 ...
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bioRxiv - Microbiology 2022Quote: ... gondii infected-PC12 cells that were harvested on day five post-infection using the Direct-zol RNA MiniPrep Plus kit (Zymo Research, USA) according to the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2022Quote: ... An equal volume of ethanol 100% was added and the mixture was then loaded into a Zymo-Spin™ IIICG Column from the Quick-RNA Miniprep Kit (Zymo Research). Further steps were conducted according to the manufacturer’s protocol ...
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bioRxiv - Bioengineering 2022Quote: ... Total RNA was then isolated from all mouse tissues using TRIzol reagent and RNA Isolation kits with on-column DNase treatment (Zymo Cat# R2072). cDNA synthesis was performed with random primers from the Protoscript cDNA synthesis kit (NEB Cat#E6560S) ...
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bioRxiv - Bioengineering 2022Quote: Plasmid DNA was extracted from frozen glycerol library aliquots using a Zyppy Plasmid Miniprep Kit according to manufacturer instructions (Zymo, Cat# D4036). After performing assembly selections on the double mutant libraries ...
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bioRxiv - Cell Biology 2022Quote: ... ethanol was added in a ratio of 1:1 to the supernatant from the previous step and continued according to the manufacturer instructions of Direct-zol mini (for tissues) and micro (for fecal wash) prep kit (ZYMO research, R2052).
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bioRxiv - Cancer Biology 2022Quote: Genomic DNA was extracted from HBEC controls and various HBEC derivative cells (three different clones for each group) using a Quick-DNA Miniprep Plus kit (Zymo Research, D4070). DNA samples were submitted to the Van Andel Institute’s Genomics Core (Controls ...
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bioRxiv - Genomics 2022Quote: ... Each well was made up to 50uL with H2O and bisulfite conversion was performed following manufacturer protocols using the EZ-96 DNA Methylation Kit (Zymo, Cat. D5004) and eluted twice (12.5 uL each using elution buffer ...
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bioRxiv - Genomics 2022Quote: ... The RNA was then extracted from both samples using the Quick-RNA MiniPrep kit following the manufacturer’s instructions (Zymo Research, Irvine, CA). Total RNA in samples was quantified using a Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Genetics 2022Quote: ... 30 ng of purified dsDNA was tagmented at 55 °C for 15 min and purified using DNA Clean & Concentrator-5 kit (Zymo Research, D4004) The tagmented DNA was PCR amplified using Nextera primers ...
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bioRxiv - Molecular Biology 2022Quote: ... Tagmentation was performed for 30 minutes at 37°Con a rotator and DNA was purified using the DNA Clean and Concentrator 5 kit (Zymo Research D4004). PCR was performed as previously described [23] using 11 total cycles ...
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bioRxiv - Systems Biology 2022Quote: ... the cells were lysed in 700uL of Qiazol and RNA was isolated using the Zymo Direct-zol Mini-prep kit and reagents (Zymo-Catalog # RD2051). RNA integrity was determined with a Bioanalyzer and only RNA samples with a RNA Integrity Number (RIN ...
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bioRxiv - Plant Biology 2022Quote: ... We then thawed the ground tissue in trizol reagent and extracted RNA using a Direct-zol 96 kit according to manufacturer’s instructions (Zymo Research, Irvine CA). Lexogen quant-seq libraries for each sample were prepared and sequenced by the Cornell Institute of Biotechnology for the maize and sorghum datasets.
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bioRxiv - Microbiology 2022Quote: ... RNA sequencing library construction and ribosomal RNA depletion were performed using the Zymo-Seq RiboFree™ Total RNA Library Kit (Zymo Research). Paired-end (150 bp ...
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bioRxiv - Plant Biology 2023Quote: ... Total RNA was extracted from the leaf samples with the Direct-zol RNA Miniprep Plus kit according to the manufacturer’s (Zymo Research Europe GmbH) guidelines ...
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bioRxiv - Neuroscience 2022Quote: ... Cross-links between the DNA fragments and histones were reversed and the DNA fragments were recovered using the ChIP DNA Clean & Concentrator Kit (Zymo, Irvine, CA). Two biological replicates were performed for each group ...
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bioRxiv - Molecular Biology 2022Quote: ... The gel was cut to purify fragments from 200 bp to 600 bp using Zymoclean Gel DNA Recovery Kit (Zymo Research, D4007). The purified DNA libraries were measured by the Qubit HS ds DNA Assay Kit (ThermoFisher ...
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bioRxiv - Immunology 2022Quote: ... Samples were either kept in −80C at this point or proceeded with standard Trizol-mediated RNA extraction protocol or the Direct-zol 96 kit (Zymo Research, R2054) was used following the on-column DNA digestion protocol ...
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Atypical epigenetic and small RNA control of transposons in clonally reproducing Spirodela polyrhizabioRxiv - Plant Biology 2024Quote: ... small RNAs ranging from 17-to-25 nt were excised and recovered using the ZR small-RNA PAGE Recovery Kit (Zymo Research, #R1070). TraPR ...
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bioRxiv - Neuroscience 2024Quote: ... A total of 750 ng of DNA was subjected to bisulfite conversion using the EZ DNA Methylation kit (Zymo Research, Irvine, USA). Genome-wide DNA methylation was then performed using the Infinium HumanMethylationEPIC Bead Chip (Illumina) ...
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bioRxiv - Microbiology 2023Quote: Viral RNA extraction from nasal washes of infected ferrets was done using the Quick-RNA Viral 96 Kit (Zymo, Cat. No. R1040) using 160 µL sample volume ...
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bioRxiv - Microbiology 2023Quote: ... The MDA-amplified VLP DNA was then processed using the Zymo Genomic DNA Clean and Concentrator-10 kit (Zymo Research, CA, U.S.A) and eluted in Zymo DNA Elution Buffer ...
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bioRxiv - Plant Biology 2024Quote: Total RNA from seedlings was isolated using a Quick-RNA MiniPrep kit with on-column DNase I treatment (Zymo Research, Irvine, CA). cDNA was synthesized using Oligo(dT ...
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bioRxiv - Physiology 2024Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick-RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were pelleted by centrifugation (500 x g for 5 minutes) and lysed for RNA and DNA extraction using the Quick-DNA/RNA Miniprep Plus kit (Zymo Research, D7003). RNA and DNA were eluted in water ...
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bioRxiv - Molecular Biology 2024Quote: ... was used to purify genomic DNA from the cells followed by bisulfite conversion using 50 ng DNA along with the EZ DNA Methylation- Gold kit (Zymo Research D5005). 10 ng of bisulfite-converted DNA was used to generate the whole genome bisulfite sequencing (WGBS ...
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bioRxiv - Microbiology 2024Quote: ... The amplified products were purified using the QIAquick PCR purification kit and underwent size selection using 1.5% low-melt agarose gels and Gel DNA Recovery Kit (Cat. No. D4002, Zymo Research, Irvine, CA). A final pool was made after quantification of each sample using the Qubit high-sensitivity dsDNA kit (Cat ...
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bioRxiv - Cancer Biology 2024Quote: RNA from K562 and Nalm6 cells was isolated using the NucleoSpin RNA Mini kit (Macherey Nagel) followed by DNase-digestion with the DNase I set (Zymo Research, #E1010) and clean-up using the NucleoSpin RNA Clean-up Mini Kit (Macherey-Nagel ...
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bioRxiv - Cell Biology 2024Quote: ... and all reactions with the appropriate molecular weight product were combined and cleaned using the DNA Clean and Concentrator 5 kit (Zymo Research D4013). 10 µg LentiCRISPRv2-opti (Addgene #163126 ...
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bioRxiv - Biochemistry 2023Quote: ... T7 transcribed SL5 RNA was first purified by polyacrylamide gel electrophoresis (PAGE) and recovered using small-RNA PAGE Recovery Kit (Zymo Research, R1070). RNase L (1.3 µg in 5 µL ...
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bioRxiv - Synthetic Biology 2024Quote: ... Samples were frozen in liquid nitrogen and mechanically homogenized before total RNA extraction was performed using the Quick-RNA™ Microprep Kit (Zymo Research). 200ng from each sample were transferred to GenTegra-RNA tubes (Syntezza Bioscience ...
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bioRxiv - Physiology 2023Quote: ... repeat 3-5 times on ice) and total RNA was extracted following the instructions of the Quick- RNA MiniPrep kit (Zymo Research, R1055) and reverse transcription was performed by SuperScript™ III (18080093 ...
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bioRxiv - Developmental Biology 2024Quote: ... according to the manufacturer’s instructions and 35 µL of the supernatant were treated with the RNA Clean & Concentrator Kit 5 (Zymo Research, Irvine, USA) using an adapted protocol (the RNA prep buffer step was performed twice ...
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bioRxiv - Genomics 2024Quote: ... RNA and DNA were simultaneously extracted from the cell samples using the Zymo Quick-DNA/RNA Microprep Plus Kit (Zymo Research, D7005) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The sgRNA target sites were amplified using PCR and purified using the DNA clean and concentrate kit (Zymo Research, Cat. No. D4033). The purified PCR amplicon products were sequenced by Eurofins Genomics ...
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bioRxiv - Cell Biology 2024Quote: Total RNA was extracted from snap-frozen cell pellets using the Quick-RNA Micro Prep Kit (Zymo Research, Irvine, CA, USA, R1050) and DNase digestion as per manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: Total RNA greater than 200 nt was purified from 100 µL of the lysates described above using the RNA Clean & Concentrator-25 kit (Zymo Research, R1017) as previously described (Hsu et al. ...
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bioRxiv - Evolutionary Biology 2023Quote: ... methylated indexed adapters were ligated and 20 µL of cleaned DNA fragments were subjected to sodium bisulfite conversion using the EZ DNA Methylation-Lightning Kit (Zymo Research, CA). Pre-amplification of the bisulfite-converted library was carried out for an equimolar pool of eight samples and was then subjected to exome capture at 47°C for 45 hours ...
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bioRxiv - Neuroscience 2023Quote: Primary cortical neurons were infected at 3 DIV with FL1 and at 10-12 DIV neurons were harvested and DNA extracted using Quick-DNA Miniprep Kit (Zymo Research, USA) following manufacturer recommendations ...
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bioRxiv - Immunology 2023Quote: ... and total cellular RNA from cardiomyocytes isolated from vehicle-and Doxo-treated mice was prepared using Direct-zol RNA miniprep kit (Zymo Research, R2052). Next-generation RNA sequencing procedure for both sets of samples were performed at Texas A&M University Bioinformatics Core ...