Labshake search
Citations for Zymo Research :
6301 - 6350 of 6691 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... PCR products were isolated from agarose gel after electrophoresis and purified using Zymoclean Gel DNA Recovery kit (Zymo Research, Irvine, CA, USA). Purified PCR fragments were ligated in pCR® II Vector – Dual Promoter TA Cloning Kit (Invitrogen-Thermo Fisher Scientific ...
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bioRxiv - Physiology 2023Quote: ... and immediately lysed with TRIzol (Fischer Scientific, 15-596-018) for RNA isolation using the Direct-zol RNA MicroPrep Kit (Zymo Research, R2062) per the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2023Quote: ... the clarified cell lysates (2×108 cells) were used to purify total RNA using the Direct-zol RNA MiniPrep kit (Zymo Research, USA). One microgram of total RNA after Turbo DNase I (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Bacterial genomic DNA was extracted from overnight cultures grown in 5 mL BHI broth using the Zymo BIOmics DNA Miniprep Kit (Zymo, Irvine, CA).
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bioRxiv - Genomics 2022Quote: ... DNA extracts from each experimental replicate without standards or phage HM1 were cleaned up prior to library preparations with the Zymo Genomic DNA Clean and Concentrate-10 kit (Cat. No. D4011, Zymo Research Corporation). Long read libraries were prepared with the Ligation Sequencing Kit (Cat ...
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bioRxiv - Genetics 2023Quote: Genomic DNA was extracted from agar plugs (as described above for PFGE chromosome preparation) using the Zymoclean gel DNA recovery kit (Zymo Research, D4001T) and successively digested with Exonuclease V (RecBCD ...
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bioRxiv - Molecular Biology 2023Quote: ... Cleaned pools of samples were gel purified to remove excess primer and eluted cDNA was cleaned through Zymo DNA Clean and Concentrator kit (Zymo Research #D4014).
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bioRxiv - Evolutionary Biology 2023Quote: ... RNA was then extracted from each sample using the Quick-RNA MiniPrep Kit according to the manufacturer’s protocol (Zymo Research, Irvine, CA). The total amount of RNA in each sample was subsequently quantified with a Qubit 2.0 fluorometer (Life Technologies ...
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bioRxiv - Cell Biology 2023Quote: ... The cells were then washed with PBS and lysed in Tri-reagent and RNA was extracted using the Direct-zol RNA miniprep kit (Zymo Research, # R2050) following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 38% v/v saturated acidic phenol) and the Direct-zol RNA MiniPrep Kit (including on-column DNase I treatment, Zymo Research, R2052) or according to the protocol for TRIzol™ Reagent (Invitrogen) ...
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bioRxiv - Biochemistry 2023Quote: ... equal amount of nuclease free water was added to each sample and purified using Oligo Clean & Concentrator kit (Zymo Research, CAT#: D4061). The samples were eluted in 6 μL of nuclease free water The samples were eluted in 6 μL of nuclease free water and an equal amount of 2x denaturing loading buffer was added for separation on a 20% denaturing polyacrylamide gel as described above ...
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bioRxiv - Genetics 2023Quote: ... Cells were cultured for 3-5 days post-transfection after which genomic DNA was extracted using the Quick-DNA Microprep Kit (Zymo Research Corporation).
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bioRxiv - Immunology 2023Quote: ... we have isolated DNA for preparing Reduced Representation Bisulfite Sequencing (RRBS) libraries using Zymo-Seq RRBS Library Kit (Zymo Research, USA, #D5461). In brief ...
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bioRxiv - Systems Biology 2023Quote: DNA was extracted from approximately 100 mg of each gut content sample using the ZymoBIOMICS™ 96 MagBead DNA Kit (D4308) (Zymo Research), following manufacturer’s recommendations ...
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bioRxiv - Neuroscience 2023Quote: ... four independent differentiations from C71G+/- iMGs and three independent differentiations from M114T+/- and M114T+/+ iMGs were lysed with Trizol and total RNA was isolated with Direct-zol RNA Microprep Kit (Zymo Research, R2060) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: In vitro-infected keratinocytes and SCC cells were lysed in 6-wells with 1mL TRIzol Reagent and the RNA was purified by using the Direct-zol™ RNA Miniprep kit (Zymo Research). The procedure was performed according to the manufacturer protocol except for an additional 1 min centrifugation after the last washing step to completely remove eventual ethanol leftover ...
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bioRxiv - Genetics 2023Quote: ... Total RNA was purified from the aqueous phase using the RNA Clean & Concentrator-5 kit with DNase I treatment to remove genomic DNA contamination (Zymo Research R1013). RNA quantity was determined with the Qubit RNA Broad Range Assay (ThermoFisher Scientific Q10210) ...
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bioRxiv - Genetics 2023Quote: ... The amplicons were treated with DpnI restriction enzyme to remove the plasmid template and transformed into XL10 gold competent cells prepared by ‘Mix and Go!’ transformation kit (Zymo Research, T3001). The sequence of the isolated plasmids was confirmed using nanopore sequencing (Plasmidsaurus ...
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bioRxiv - Genetics 2023Quote: RNA was extracted from each of the 384 samples in batches of 30 samples using the Direct-zol RNA Miniprep kit (Zymo Research, R2050). Batches consisted of samples from the same tissue and were processed in the order individual samples were collected ...
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bioRxiv - Neuroscience 2023Quote: ... We extracted DNA from the whole fly or from just the body (saving the heads for immunostaining from a subset of flies) using Quick-gDNA miniprep kit (Zymo Research R1050). We designed primers upstream and downstream of the Tra binding sites as follows:
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bioRxiv - Molecular Biology 2023Quote: Genomic DNA from transduced primary cortical neurons or brain tissue from transduced animals was extracted using Quick-DNA Miniprep Kit (Zymo Research, USA) following manufacturer recommendations ...
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bioRxiv - Genomics 2023Quote: ... bisulfite sequencing libraries were constructed according to the manufacturer’s instructions using the EZ DNA Methylation-GoldTM Kit (Zymo Research, Irvine, CA, USA). Bisulfite sequencing libraries were sequenced to generate 150 bp paired-end reads ...
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bioRxiv - Genomics 2023Quote: ... 200 ng of sonicated DNA was subjected to bisulfite conversion using the EZ DNA Methylation-Gold™ Kit (Zymo, Cat No. D5006). Libraries were then made using the Accel-NGS Methyl-Seq DNA Library Kit with unique dual indices following the size-selection guidance provided with the kit and 10 cycles of amplification to minimise clonality ...
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bioRxiv - Bioengineering 2023Quote: ... and homogenized further using the mixer mill MM400 (Retsch) followed by RNA isolation with the Direct-zol™ DNA/RNA Miniprep Kit (Zymo Research). Total RNA concentrations were measured using the NanodropTM 2000c spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Bioengineering 2023Quote: ... The transcribed product was treated with DNase I for 30 min at 37°C and then purified using RNA Clean and Concentrator Kit (Zymo Research #R1016).
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bioRxiv - Bioengineering 2022Quote: ... The PCR amplified DNAs were purified by DNA Clean and Concentrator or Zymoclean™ Gel DNA Recovery Kit (Zymo Research, Irvine, CA). Primers were synthesized by Integrated DNA Technologies (Coralville ...
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bioRxiv - Microbiology 2022Quote: ... was added to each well followed by isolation of total RNA using Direct-Zol RNA Mini-Prep Plus Kit (Zymo Research, R1058) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... genomic DNA was isolated from HL-7702 cell lines and 2 μg DNA was treated with sodium bisulfite using an EZ DNA Methylation-Gold Kit (ZYMO Research, USA) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The crosslinking was reversed at 65 °C and the samples were purified using the ChIP DNA Clean and Concentrator kit (Zymo Research, D5205). The DynaMagTM-2 Magnet (Thermo Fisher Scientific ...
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bioRxiv - Genomics 2023Quote: ... These samples were used as the starting material for an RNA extraction using the Quick-RNA Viral Kit (Zymo, Cat no: R1034). The final elution was collected in 15 μl of nuclease-free water.
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bioRxiv - Cell Biology 2023Quote: ... was extracted from podocytes of Ercc1/Nphs2.Cre/mTmG mice using Direct-zol™ RNA MiniPrep Kit (cat. no. R2052, Zymo Research). Isolation of glomeruli ...
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bioRxiv - Plant Biology 2023Quote: ... Each biological replicated was generated by pooling three individual ChIP pull-downs (each consisting of 6 g of whole seedling tissues) during DNA purification using the ChIP DNA Clean & Concentrator Kit (Zymo Research, D5201). The samples were sent to BGI Genomics for library preparation and sequencing using DNBSEQ ...
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bioRxiv - Neuroscience 2023Quote: ... RNA from three biological replicates of vehicle-treated (Veh) and 48 h-TTX-treated (TTX) neurons (6 samples in total) were isolated using Quick-RNA Microprep kit (Zymo Research, R1051) according to the instructions of manufacturer ...
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bioRxiv - Systems Biology 2023Quote: ... The aqueous phase was transferred to another tube by pipette and cleaned up using a Zymo cleanup and concentrator kit (Zymo Research D4013), then subject to size selection using SPRI-select beads (Beckman Coulter B23317 ...
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bioRxiv - Microbiology 2023Quote: ... DNA bands at ∼380 bp were excised and purified utilizing the Zymo Gel DNA Recovery Kit (Zymo Research, Irvine, CA, United States). Purified PCR products were equimolar pooled for a final library concentration of 11 pmol/l ...
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bioRxiv - Cell Biology 2023Quote: ... The sorted nuclei were then pelleted at 3000 × g for 10 minutes before mRNA isolation using the Direct-zol RNA Microprep kit (Zymo research R2060). All RNA samples were initially amplified using the Ovation RNA-Seq System v2 kit (Tecan Genomics) ...
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bioRxiv - Immunology 2023Quote: SARS-CoV-2 vRNA was isolated from BAL supernatant (200 µL) and mucosal swabs (nasal, pharyngeal, and rectal) using the Zymo Quick-RNA Viral Kit (Zymo Research, USA) as previously described [27,29] ...
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bioRxiv - Physiology 2023Quote: Total RNA was extracted from the livers or adipose tissue using Direct-zol RNA Microprep Kits (R2061, Zymo Research, Irvine, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2023Quote: Antibody genes were isolated from the terminal rounds of sorting against tau and α-synuclein fibrils using a yeast miniprep kit (Zymo, D2004). For tau ...
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bioRxiv - Developmental Biology 2023Quote: ... PCR were performed and PCR amplicons were used to conduct gel running and purified by DNA Clean & Concentrator kit (Zymo Research, # D4031). A-tailing were then performed on the purified PCR products following the NEB protocol ...
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bioRxiv - Molecular Biology 2023Quote: ... between 400 million to 1.2 billion cells (two to six flash-frozen pellets) were lysed and extracted using the YeaStar Genomic DNA kit (Zymo 11-323), following the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2023Quote: ... The RNA was then isolated from the membrane using phenol-chloroform and concentrated with the RNA Clean and Concentrator kit (Zymo Research, R1013), followed by reverse transcription using the RT Oligo primer (Supplementary Data 12 and SuperScript III ...
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bioRxiv - Plant Biology 2023Quote: ... and prey (pGADT7) constructs were co-transformed into the yeast strain AH109 using Frozen-EZ Yeast Transformation II Kit (Zymo Research, T2001). The resulting transformants were spotted on SD (−Leu ...
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bioRxiv - Genomics 2023Quote: ... and dissected tissues (head, abdomen, gonads) from adult unparasitised males and females using a Direct-zol RNA Microprep kit (Zymo Research, USA), including DNAse treatment following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: ... in Lysing Matrix B tubes and RNA was purified with the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA, United States). The quantity and quality of the RNAs were evaluated using Qubit RNA HS Assay Kit (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2023Quote: Samples were homogenized in an Omni Bead ruptor in TRIzol and then the RNA was extracted from 300μL of each sample using the Direct-zol RNA miniprep kit (Zymo Research, Irvine, CA). 2μL of isolated RNA from each sample was then converted to cDNA using the RevertAID first strand cDNA synthesis kit (Thermo Fisher ...
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bioRxiv - Neuroscience 2023Quote: ... The sequencing libraries were then run on a 10% TBE polyacrylamide gel and purified using the DNA Clean & Concentrator-5 kit (Zymo Research D4013).
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... ligation products were cleaned using AMPure XP Beads (Fisher, Cat. #NC9933872) before processing for bisulfite conversion using the Zymo EZ DNA Methylation Kit (Zymo, Cat. #D5001), and by amplifying the bisulfite converted products over 55 cycles of 95°C for 30 seconds followed by 55°C for 15 minutes ...
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bioRxiv - Molecular Biology 2023Quote: ... Total RNA from line with Bon GLKD driven by MT-Gal4 (and control flies that express an shRNA against the white gene) was depleted of ribosomal RNA with the Zymo-Seq RiboFree Total RNA Library Kit (Zymo Research, #R3000). RNA-seq libraries were made using the NEBNext Ultra II Directional RNA Library Prep kit for Illumina (NEB ...
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bioRxiv - Genomics 2023Quote: ... Single colonies were used to inoculate ampicillin liquid cultures grown overnight with shaking at 37°C and plasmids were subsequently purified with the ZR Plasmid Miniprep kit (Zymo Research #D4016). The purified plasmids were screened by Sanger sequencing (GeneWiz ...