Labshake search
Citations for Zymo Research :
5701 - 5750 of 6691 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... The reaction was stopped by adding 250 µl of DNA Binding Buffer and DNA was isolated using the Clean and Concentrator-5 Kit (Zymo). Libraries were produced by PCR amplification of tagmented DNA and sequenced on a NextSeq 2000 sequencer 150 bp Paired End.
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bioRxiv - Zoology 2024Quote: DNA from the homogenized termite guts was extracted using the Quick-DNA™ Fecal/Soil Microbe Microprep Kit (Zymo Research) following kit instructions ...
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bioRxiv - Systems Biology 2024Quote: ... and then each sample was applied to a spin column supplied in the RNA clean and concentrator kit (Zymo Research). Samples were centrifuged at 12,500 rcf for 30 seconds ...
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bioRxiv - Microbiology 2024Quote: DNA was extracted from pelleted cells as described previously16 using a ZymoBiomics DNA Miniprep kit (Zymo Research; Irvine, CA, USA) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: Total RNA was extracted by Direct-zol RNA Miniprep Plus kit according to the manufacturer’s instruction (Zymo, Catalog no. R2072) and then reverse-transcribed into complementary DNA using the iScript Reverse Transcription Supermix (Bio-Rad ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were treated as described and RNA was extracted using the Zymo Quick-RNA MiniPrep Kit (Zymo Research, catalog R1055) following the kit instructions ...
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bioRxiv - Genomics 2024Quote: ... and a pool of L3 larvae (A. caninum) using a Quick-DNAÔ HMW MagBead Kit (Zymo Research, Irvine, CA, USA) and modified Solid Tissue extraction protocol (Supplemental Table 1) ...
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bioRxiv - Molecular Biology 2024Quote: ... Products from PCR1 were run on a 2% agarose gel in TAE (Tris-acetate-EDTA) buffer and fragments were extracted using the Zymo Gel DNA recovery Kit (Zymo). Eluted products were pooled in equimolar amounts and were submitted to the UC Davis DNA Technologies and Expression Analysis Core lab for a second round of PCR (PCR2 ...
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bioRxiv - Microbiology 2024Quote: ... The product of this reaction was then run on a 0.8% agarose gel and purified using Zymoclean Gel DNA Recovery Kit (Zymo Research) to yield the final Spike DNA fragment ...
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bioRxiv - Plant Biology 2020Quote: ... and RNA greater than 17 nt was isolated following manufacturer’s instructions with the Zymo RNA clean and concentrator kit (Zymo Research; R1017). After checking digestion quality ...
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bioRxiv - Plant Biology 2020Quote: ... RNA less than 200 nt was isolated following manufacturer’s instructions with the Zymo RNA clean and concentrator kit (Zymo Research; R1015). Next ...
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bioRxiv - Microbiology 2020Quote: ... and mRFP1 was PCR amplified using primers listed in Table 3 and purified using DNA Clean and Concentrator-5 kit (Zymo Research). pUC19 was digested with PstI and pBAD18 was digested with EcoRI and HindIII ...
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bioRxiv - Synthetic Biology 2021Quote: ... Bands of the expected size were cut out and DNA was extracted with a ZymoClean Gel DNA recovery kit (Zymo Research). For all cloning steps ...
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bioRxiv - Cancer Biology 2021Quote: Sorted cKithighCD11blow AML cells were lysed in Trizol reagent and RNA was extracted using Direct-zol RNA miniprep kit (Zymo Research) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2021Quote: ... the RNA volume was brought up to 100 μL with nuclease-free water and was purified away from other reaction components by an RNA Clean & Concentrator kit (Zymo Research) by following the manufacturer’s instructions ...
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bioRxiv - Biophysics 2020Quote: ... The completed PCR reactions were pooled and ethanol-precipitated prior to purification using a DNA Clean & Concentrator Kit (DCC-25, Zymo Research). The concentration of the labeled 197-bp PCR products was determined by UV absorbance ...
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bioRxiv - Cell Biology 2020Quote: ... and the RNA was extracted using the Direct-zol™ RNA purification kit following the manufacturer’s protocol for DNase I digestion (Zymo Research). cDNA was synthesized with Maxima Reverse Transcriptase (Thermo Scientific™ ...
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bioRxiv - Cell Biology 2020Quote: ... The parasites stages were homogenized in TRIzol with DNA and total RNA was extracted using the Direct-Zol RNA MiniPrep Plus kit (Zymo Research) according to manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: ... The 300 bp sized Micro-C library was purified via 1.5 % agarose gel electrophoresis and extracted with Zymoclean Gel DNA Recovery Kit (Zymo Research, D4002) with a final elution volume of 50 μL ...
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bioRxiv - Genetics 2021Quote: ... We extracted and isolated RNA by phase separation using standard protocols followed by purification using the Direct-zol RNA microprep kit (Zymo R2060) with an on-column DNAse step per manufacturer directions ...
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bioRxiv - Microbiology 2019Quote: ... The enriched circular DNA samples were then purified by DNA Clean & ConcentratorTM-5 kit according to manufacturer’s instructions (Zymo Research, USA). Elution was done with 10 µl of 10 mM Tris (pH 8.0) ...
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bioRxiv - Evolutionary Biology 2021Quote: ... PCR products were resolved on 2% agarose gels followed by extraction and purification of candidate DNA amplicons using Zymoclean Gel DNA Recovery Kits (Zymo Research). Purified PCR products were cloned using the TOPO TA cloning kit (ThermoFisher Scientific) ...
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bioRxiv - Evolutionary Biology 2020Quote: Genomic DNA from approximately 105 − 106 cells was extracted from the cultures of the evolution experiments every 48 hours using Quick-DNA miniprep kits (Zymo Research). The HIV RT gene amplified by PCR from the proviral RNA using Phusion hot start II DNA polymerase (New England Bio-labs ...
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bioRxiv - Genomics 2021Quote: ... Fragments between 400-700 bp were excised from the gel and recovered using the ZymoClean Gel DNA Recovery Kit (Zymo Research). The final libraries were quantified using the Qubit dsDNA BR Kit (Thermo Fisher ...
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bioRxiv - Developmental Biology 2021Quote: ... Duplicate PCR reactions of each sample were carried out and the amplified products were pooled using a DNA Clean and Concentrator kit (Zymo Research). The resulting amplified fragments were analyzed via Sanger sequencing using both forward and reverse primers ...
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bioRxiv - Developmental Biology 2021Quote: cDNA preparation and preamplification: RNA isolation of digested samples was performed using the Quick-RNA MicroPrep kit (Zymo Research, Cat #: R1050). In-column DNAse-I treatment was performed for trace DNA removal before eluting RNA ...
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bioRxiv - Developmental Biology 2021Quote: ... the four guide RNA templates were pooled together and concentrated through purification by a DNA Clean and Concentrator kit (Zymo Research).
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bioRxiv - Synthetic Biology 2022Quote: ... carrying integrated copy constitutively expressed mCherry reporter was used to prepare competent cells and transformation of plasmids using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Sequences of synthetic DNA and strains used in this study are shown in Data S1 and Table S2.
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bioRxiv - Molecular Biology 2022Quote: ... the reaction was stopped by adding MN Stop Buffer and the nucleosomal DNA isolation was carried out according to the kit protocol (D5220, Zymo Research). Illumina TruSeq PCR free DNA library was prepared without bead-based size selection followed by a preparative size selection on the PippinHT to remove non- ligated adaptors and library molecules with inserts >500 bp.
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bioRxiv - Molecular Biology 2022Quote: ... TurboDNase treatment as described above usually removes most genomic DNA and small fragments of DNA that could prime as random primers (detectable by ‘no RT primer’ control) are cleared away efficiently by the RNA Clean & Concentrator-5 kit (Zymo Research). Hence ...
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bioRxiv - Molecular Biology 2022Quote: ... We followed the PCR with an agarose gel extraction and a column purification using the Oligo Clean & Concentrator kit (Zymo Research). The DNA template was then in vitro transcribed using the HiScribe T7 high yield RNA synthesis kit (NEB ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... were homogenized in RNA STAT-60 (Tel-Test, Inc., Friendswood, TX) and total RNA purified using a Direct-zol RNA miniprep kit (Zymo Research). We used an RNA Clean and Concentrator Kit (Zymo Research ...
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bioRxiv - Neuroscience 2021Quote: ... we used Q5 hot-start high-fidelity DNA polymerase to amplify the transcripts of each candidate gene from complementary DNA (cDNA) synthesized using the total RNA of w1118 fly heads (extracted using MiniPrep kit; Zymo Research). For each candidate gene ...
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bioRxiv - Molecular Biology 2021Quote: RNA was extracted from 150 µL of the fractionated samples using an RNA clean and concentrator-5 kit (Zymo Research, R1016) using the same procedure to extract RNA from unfractionated cell lysate (described above) ...
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bioRxiv - Molecular Biology 2020Quote: ... The resulting product was then treated with Turbo DNase and the remaining RNA was purified with the Zymo RCC-25 column purification-25 kit (Zymo Research). The A/B RNA standards were quantified using Qubit RNA HS Assay on Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Neuroscience 2021Quote: ... and fl/fl from each nest/uterus) that were subsequently cleaned and DNAse treated using the zymo-clean&concentrator kit (Zymo Research). For primary neurons ...
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bioRxiv - Molecular Biology 2021Quote: ... The gel band corresponding to the size of dinucleosomal DNA (∼250 to 350 bp) was cut and purified with the Zymoclean Gel DNA Recovery Kit (Zymo #D4008). We note that size selection for DNA larger than 200 bp greatly reduces the ratio of unligated monomers in Micro-C data.
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bioRxiv - Molecular Biology 2021Quote: ... 10 fully grown GV oocytes (an equivalent of 40 haploid genomes and 80 single-stranded DNAs after bisulfite conversion) were directly subjected to EZ DNA Methylation-Direct kit (Zymo Research) for bisulfite conversion with the following modifications ...
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bioRxiv - Molecular Biology 2021Quote: High-molecular-weight genomic DNA from HAP1 Δ72 and HepG2 Δ15 deletion clone was extracted using Quick-DNA kit (Zymo Research) and shipped to Samplix Services (Denmark ...
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bioRxiv - Bioengineering 2022Quote: ... cerevisiae strain EBY100 (ATCC MYA-4941) (Boder & Wittrup, 1997) using frozen-EZ yeast transformation II kit (Zymo Research, Cat. No. T2001) and grown on SD-CAA agar plates for 3 to 4 days ...
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bioRxiv - Cell Biology 2022Quote: All above constructs were transformed into Top10 competent cells prepared using Mix & Go E.coli Transformation Kit and Buffer set (Zymo Research #T3002), cultured on LB agar plates to select for antibiotic resistance using standard workflows for molecular cloning and DNA production 46 ...
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bioRxiv - Genomics 2020Quote: ... 5 µl reactions were purified with the ZR-96 Oligo Clean & Concentrator and 15 µl reactions were purified with the Oligo Clean & Concentrator kit (Zymo Research) and eluted with probe resuspension buffer ...
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bioRxiv - Genomics 2020Quote: ... The gel band corresponding to the size of dinucleosomal DNA (∼250 to 350bp) was cut and purified with Zymoclean Gel DNA Recovery Kit (Zymo #D4008). We note that the size-selection for DNA larger than 200bp greatly reduces the ratio of unligated monomer in Micro-C data.
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bioRxiv - Developmental Biology 2020Quote: ... Samples were re-suspended with 50 μL of nuclease-free water and subsequently purified using the RNA Clean & Concentrator kit (Zymo, R1018). The concentration of each sample was determined using a Nanodrop spectrophotometer ...
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bioRxiv - Genomics 2020Quote: ... the adaptor-ligated MspI fragments were bisulfite-treated for two consecutive rounds using Zymo’s EZ DNA Methylation-Gold Kit (Zymo cat. D5005) per the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... The reverse cross-linked samples were treated with 20 μg/ml Proteinase K and purified using ChIP DNA Clean and Concentrator kit (Zymo research). The three reactions per condition were pooled at this stage ...
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bioRxiv - Molecular Biology 2021Quote: ... one each with the insert in genomic and in antigenomic orientation for plasmid stock preparation using ZymoPURE II Plasmid Maxiprep Kit (Zymo Research).
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bioRxiv - Cell Biology 2019Quote: The presence of the point mutations in the genome of the clones was confirmed by extraction of genomic DNA using Quick-DNATM Universal Kit (ZYMO Research) followed by amplification of the N-terminal of BRCA2 (aa 1-267 ...
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bioRxiv - Cell Biology 2019Quote: ... Genotyping PCR was performed using Taq polymerase on genomic DNA extracted from SMB55 cells using ZymoBead™ Genomic DNA kit (Zymo). Genomic DNA extracted from Ptch1-/- mouse embryonic fibroblasts and a wild-type mouse were used as a control for the mutant and wild-type alleles respectively ...
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bioRxiv - Neuroscience 2019Quote: ... The gDNA of the sorted cells (both N2A and NSCs) was extracted with the Genomic DNA(tm) - Tissue MiniPrep kit (Zymo Research) following the protocol of the manufacturer for cell suspensions ...