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Citations for Zymo Research :
5801 - 5850 of 6691 citations for Glutathione S Transferase GST Fluorescent Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... and from bacteria harvested from broth cultures were extracted with ZymoBIOMICS™ DNA Miniprep or Microprep kits (Zymo Research, Irvine, CA). Freshly-dissected ...
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bioRxiv - Microbiology 2019Quote: ... 5 µL of ligated plasmid was then transformed into competent BW23473 cells (made using the Mix & Go E. coli Transformation Kit & Buffer Set, Zymo Research) and plated on LB + amp plates and grown overnight ...
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bioRxiv - Molecular Biology 2021Quote: ... Duplexed 12RSS4-9 was digested with MluI and EcoRI and purified using the Zymo DNA Clean and Concentrator Kit (Zymo # D4013). The double-digested and gel purified pMX-INV and duplexed 12RSS4-9 were ligated at a 3:1 12RSS4-9:pMX-INV ratio with T4 DNA Ligase by overnight incubation at 16°C ...
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bioRxiv - Molecular Biology 2021Quote: ... The reactions were treated with DNAse (15 min at 37°C) and remaining RNA was recovered using Direct-zol RNA mini prep kit (Zymo research) according to the protocol ...
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bioRxiv - Molecular Biology 2021Quote: RNA was isolated from cultured cells using BIO TRI RNA reagent (Bio-lab) and Direct-zol RNA MiniPrep kit (Zymo research), as instructed by the manufacturer ...
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bioRxiv - Microbiology 2020Quote: ... PCR reactions yielding single amplicons as judged by agarose gel electrophoresis were purified using a PCR clean up kit (Zymo Research) and sent for DNA sequencing ...
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bioRxiv - Genomics 2020Quote: ... About 300-500 bp DNA were selected using agarose gel electrophoresis and were purified by the Zymoclean gel DNA recovery kit (Zymo Research). High Sensitivity DNA Chips of Agilent Bioanalyzer 2100 were utilized to check library quality ...
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bioRxiv - Genomics 2021Quote: ... Clones were verified by Sanger sequencing (Quintara Biosciences) prior to retransformation and maxiprep using the ZymoPURE II Plasmid Maxiprep Kit (Zymo Research).
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bioRxiv - Genetics 2019Quote: Four 7 micron sections of the left ventricle were used to isolate total RNA using the Quick-RNATM FFPE kit (Zymo Research). 1 µg of total RNA was used to generate cDNAs using the SuperScript™ VILO™ cDNA Synthesis Kit ...
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bioRxiv - Cell Biology 2020Quote: Total RNA was extracted from OSC:eFH-piwi and wild type (WT) OSC using Trizol and Direct-zol RNA MiniPrep kit (Zymo Research, R2051). Complementary DNA (cDNA ...
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bioRxiv - Cancer Biology 2021Quote: ... The cells were then cultured for 6 days prior to RNA isolation using the ZR-Duet DNA/RNA MiniPrep Plus Kit (Zymo D7003). RNA quality and concentration were confirmed on an Agilent TapeStation and Life Technologies Qubit ...
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bioRxiv - Cancer Biology 2022Quote: ... Individual 50 μL reactions were run on a 3% agarose gel and libraries were purified using the Zymoclean Gel DNA Recovery kit (D4007, Zymo Research). Sequencing was performed using standard Illumina instructions ...
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bioRxiv - Immunology 2022Quote: ... Plasmid DNA was extracted from ten million yeast from each round of selection using the Zymoprep Yeast Miniprep Kit (Zymo Research), following manufacturer instructions ...
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bioRxiv - Microbiology 2022Quote: ... and stored at -20°C until (≤ 3 days) the DNA was extracted via the Zymo Quick-DNA Fecal/Soil Microbe kit (Zymo Research) according to the manufacturer’s protocol.
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bioRxiv - Cell Biology 2022Quote: Testing and control combinations of plasmids were co-transformed into yeast AH109 strain using the Frozen-EZ Yeast Transformation II Kit (ZYMO, T2001) and cultured on a SD/-Leu-Trp selective plate at 30 °C for 2–4 days ...
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bioRxiv - Biochemistry 2022Quote: ... which were then grown in liquid culture overnight and purified using the ZymoPure endotoxin-free midiprep kit (Zymo Research, Irvine, CA). The Bxb1 recombinase expression vector (pCAG-NLS-HA Bxb1 ...
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bioRxiv - Cell Biology 2022Quote: ... Cellular RNA was isolated by either phenol/chloroform extraction or using the Direct-zol RNA Microprep kit (Zymo Research, Irvine, CA). RT-qPCR was performed in 384 well plates on an Applied Biosystems 7900HT Fast Real-Time PCR system using the QuantiTect Virus Kit (Qiagen ...
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bioRxiv - Molecular Biology 2022Quote: ... All the steps from here were optimized from the standard protocol provided with the EZ Nucleosomal DNA Prep Kit (D5220, Zymo Research). Briefly ...
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bioRxiv - Plant Biology 2022Quote: Extraction of total RNA from Arabidopsis seedlings and Marchantia spores was carried out using the Direct-Zol RNA MINIprep kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2022Quote: The 2 final 15kb fragments were gel extracted via agarose gel electrophoresis and purified with the Large Fragment DNA Recovery Kit (Zymo Research) and eluted in water ...
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bioRxiv - Microbiology 2022Quote: ... The cells and medium were then collected and subjected to RNA extraction using the Direct-zol RNA MiniPrep kit (Zymo Research) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2022Quote: ... The remaining PCR reaction was run with the determined cycle number with the same cycler conditions as before and DNA was purified with Zymo ChIP clean and concentrator kit (Zymo research) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: DNA was obtained from at least 107 yeast per round of selection using a Zymoprep Yeast Plasmid Miniprep II kit (Zymo Research). Primers were designed to amplify the Aga2 leader peptide through to the beginning of the β2M sequence ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed three times with sterile PBS and total RNA was isolated using the Direct-Zol Miniprep Plus kit (Zymo Research) following the manufacturer’s protocol ...
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bioRxiv - Microbiology 2022Quote: ... 16S rRNA sequencing libraries were prepared by amplifying and barcoding the V1-V2 region of the 16S rRNA gene using the Quick-16S NGS Library Prep Kit (Zymo Research). Samples were pooled and sequenced on the Illumina MiSeq platform with 2 × 250 base pair standard run at Washington University DNA Sequencing Innovation Lab.
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bioRxiv - Bioengineering 2022Quote: The isolated yeast populations obtained after round 4 were subjected to colony screen and plasmid recovery by using Zymoprep kit (Zymo Research). The plasmids were transformed into E ...
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bioRxiv - Bioengineering 2022Quote: ... The PCR product was run on a 1% Agarose gel and the band at the appropriate size was excised and the DNA extracted using the Zymoclean Gel DNA Recovery kit (Zymo Research). Purified insert DNA was cloned into the linearized modRNA plasmid (5MCS3)(K et al. ...
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bioRxiv - Bioengineering 2022Quote: ... The bands of interest were cut from the denaturing gels and purified using ZR small-RNA PAGE Recovery Kit (Zymo Research). The purified RNA samples were stored in nuclease free water at -20 °C.
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bioRxiv - Immunology 2022Quote: ... Cells were sorted into TRIzol (Thermo-Fisher) and RNA was isolated using a Direct-zol RNA-mini kit with RNA clean and concentrator columns (Zymo Research) and treated with DNase (Qiagen).
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bioRxiv - Cancer Biology 2022Quote: ... RNA from either CD34+ cells sorted after transduction or bulk cells at day 12 of culture was extracted using Direct-Zol RNA MicroPrep Kit (Zymo Research) and reverse transcription was performed with SuperScript Vilo cDNA Synthesis Kit (Invitrogen) ...
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bioRxiv - Neuroscience 2021Quote: ... An equal volume of 100% ethanol (800uL) was added immediately and mixed well before proceeding to RNA isolation with the Direct-zol RNA Microprep Kit (Zymo Research). DNase treatment and RNA isolation proceeded according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Sequencing libraries were made from the Zymo mock community using the LoopSeq 16S long-read kit (referred to throughout as ‘Zymo-Loop’). This preparation was also followed for the ATCC-gut and ATCC-oral communities as well as the uterine lavage and cyanobacterial enrichment samples ...
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bioRxiv - Plant Biology 2021Quote: ... The RNA precipitants were treated with 100 μl of 0.04 U Turbo DNase I at 25°C for 30 min to remove DNA contamination and cleaned using an RNA Clean & Concentrator-5 kit following the manufacturer’s protocol (Zymo Research, USA). The RNA was eluted in 16 μl RNase-free water for subsequent experiments ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were harvested in TRIzolTM LS reagent (Invitogen) and total RNA was extracted using the Direct-Zol RNA kit (Zymo Research) according to manufacturer instruction ...
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bioRxiv - Microbiology 2021Quote: ... DNA with a size of at least ~200 bases was excised and purified using a gel DNA recovery kit (Zymo Research) with elution in 50 μl of nuclease-free water ...
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bioRxiv - Neuroscience 2021Quote: ... The transposed DNA fragments were further amplified and barcoded (Buenrostro et al., 2013, 2015) and purified with a ChIP DNA Clean & Concentrator kit (Zymo, D5205). The fragments were run on 2% E-Gel™ EX agarose gels (Thermo Fisher scientific ...
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bioRxiv - Cell Biology 2021Quote: ... the RNA was cleaned to remove enzyme and buffer components using the RNA Clean & Concentrator-5 kit (Zymo Research, Cat# R1014) before the next treatment ...
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bioRxiv - Cell Biology 2021Quote: Cellular and tissue total RNA was extracted using TRI reagent and Direct-zol RNA MiniPrep Plus kit (Zymo Research, Irvine, CA). First-strand cDNA was synthesized using the SuperScriptTM IV VILOTM cDNA synthesis kit (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2020Quote: ... Combination of the two plasmids encoding the desired fusion proteins were transformed into Y2HGold cells using Frozen-EZ Yeast Transformation II Kit (Zymo Research). Selection for growth was carried out on selective drop-out plates lacking leucine and tryptophan (SD-Leu-Trp ...
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bioRxiv - Microbiology 2020Quote: ... The presence of inserted sequences in generated vaccine virus stocks was confirmed by RNA extraction (Direct-zol RNA kit, Zymo Research) followed by RT-PCR (qScript XLT ...
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bioRxiv - Immunology 2020Quote: ... the DNA was subjected to bisulfite conversion and PCR-based sequencing adapter tagging as described for WGBS above (Pico Methyl-Seq Library Prep Kit, Zymo Research). The library was quantified and sequenced on an Illumina Nextseq 550 ...
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bioRxiv - Genomics 2020Quote: Sodium bisulfite conversion: 200 ng of NA12878 DNA was converted using the EZ DNA Methylation-Gold Kit (ZYMO Research, Irvine, CA) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2020Quote: ... Reactions were quenched with 8 volumes of ethanol and the DNA was recovered by using the DNA Clean and Concentrator kit (Zymo Research). DNA samples were digested to nucleosides using the Nucleoside Digestion Mix (NEB ...
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bioRxiv - Immunology 2021Quote: Overnight cultures of MACS sorted antibody-escaped and ACE2 preselected yeast populations were proceed to yeast plasmid extraction kit (Zymo Research). PCRs were performed to amplify the N26 barcode sequences as previously described13 ...
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bioRxiv - Molecular Biology 2020Quote: ... The linear A/B standards were finally treated with Proteinase K and further purified with the Zymo ChIP DCC-25 purification kit (Zymo Research). The final A/B standards were quantified using Qubit dsDNA HS Assay on Qubit 2.0 Fluorometer (Life Technologies ...
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bioRxiv - Immunology 2020Quote: ... Product bands of the correct size (∼400 bp) were gel-purified using the Zymoclean™ Gel DNA Recovery Kit (Zymo, D4008) and subsequently analyzed by capillary electrophoresis (Fragment analyzer ...
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bioRxiv - Microbiology 2021Quote: ... rRNA depleted RNA was purified from the reaction mixture with the Zymo RNA Clean & Concentrator-5 kit (Zymo Research, Irvine, CA) according to manufacturer instructions and eluted in 12 µl nuclease-free water ...
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bioRxiv - Immunology 2021Quote: ... RT-PCR products containing linked TCRα:β cDNA amplicons were purified using a DNA Clean & Concentrator Kit (D4033, Zymo Research, Irvine, CA).
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bioRxiv - Molecular Biology 2022Quote: ... 4sU-labelled mouse and Drosophila cells were mixed and RNA was extracted using the Direct-zol DNA/RNA Miniprep kit (Zymo Research) as per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... Samples were then de-crosslinked by incubation at 94C for 20 mins and subsequently lysed and extracted for genomic DNA (gDNA) using the Quick-DNA/RNA Miniprep Kit (Zymo D7005T). gDNA samples of both samples were sent to Cellecta Inc ...