Labshake search
Citations for Zymo Research :
5501 - 5550 of 6633 citations for Parallel Artificial Membrane Permeability Assay PAMPA Kit since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cells were lysed in Tri-reagent and RNA was extracted using the Direct-zol RNA miniprep kit (Zymo Research, # R2050) following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2023Quote: ... for DNA receiver PCR product purification and Quick-RNA 96 Kit for guide RNAs (gRNAs) purification were purchased from Zymo Research (Irvine ...
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bioRxiv - Microbiology 2023Quote: ... and the total RNA was extracted from each sample using the Direct-zol RNA MiniPrep Kit (Zymo Research, Irvine, CA). All total RNA from three biological replications of each sample were sent to the Oklahoma Medical Research Foundation Genomics Core Laboratory for the library preparation and RNA sequencing ...
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bioRxiv - Cancer Biology 2023Quote: ... These spike-in samples then undergone bisulfite conversion and purification by EZ DNA Methylation-Gold Kit (Zymo Research, D5006, USA). DNA library was prepared from bisulfite-converted DNA samples using xGen Methyl-Seq DNA Library Prep Kit (Integrated DNA Technologies ...
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bioRxiv - Microbiology 2022Quote: ... The 190 samples were extracted in the summer of 2021 using ZymoBionics DNA miniprep Kit (Zymo Research, Irvine, California, USA). We used the standard extraction protocol with modifications ...
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bioRxiv - Microbiology 2022Quote: ... and gDNA was extracted from cell pellets by the CTAB-lysozyme method (80) and cleaned using the Genomic DNA clean and concentrator kit (Zymo) before submitting to UMGC for Tn-seq library preparation and Illumina sequencing.
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bioRxiv - Molecular Biology 2022Quote: ... Ligated RNA samples with unique barcodes were pooled and cleaned up using the Oligo Clean & Concentrator kit (Zymo Research, #D4060). All samples were next reverse transcribed by using Superscript III (Invitrogen ...
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bioRxiv - Molecular Biology 2022Quote: ... Linearized plasmid DNAs and RNA bait DNAs were kit purified prior to IVTT (DNA Clean and Concentrator; Zymo Research, D4004). All DNAs were eluted into Tris-EDTA (TE ...
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bioRxiv - Molecular Biology 2022Quote: RNA from resting B cells isolated from three wild-type animals was isolated using Direct-zol RNA Miniprep Kit (Zymo). Libraries were constructed using the Illumina TruSeq Stranded Total RNA Library and sequenced on NextSeq2000 with PE100 sequencing ...
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bioRxiv - Microbiology 2022Quote: ... erythrocytes were lysed in 0.15% saponin before RNA was extracted from parasite pellets using a Direct-zol RNA Miniprep Kit (Zymo Research) according to the manufacturer’s instructions ...
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bioRxiv - Plant Biology 2022Quote: ... and total RNA was isolated using a Direct-zol RNA Miniprep Plus Kit following the manufacturer’s instructions (ZYMO RESEARCH; USA). Briefly ...
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bioRxiv - Plant Biology 2023Quote: RNA was extracted from 3 biological replicates of 100mg of frozen tissue of each accession using the Quick-DNA/RNA Miniprep kit (Zymo) following manufacturer’s instructions with the following modifications ...
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bioRxiv - Plant Biology 2024Quote: ... we sorted into 20 μl of RNA-Later then used the magnetic bead based Direct-zol-96 MagBead RNA-Isolation kit (Zymo) to isolate RNA ...
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bioRxiv - Cell Biology 2024Quote: ... The cells were scraped into solution and total RNA was purified using Zymo Quick-RNA Miniprep kit (Zymo Research, R1054). Of the isolated total RNA ...
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bioRxiv - Biochemistry 2024Quote: Total RNA was extracted using hot acid phenol method followed by purification using Direct-zol RNA Miniprep Kit (Zymo Research). The RNA was treated with DNaseI ...
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bioRxiv - Genetics 2024Quote: ... The library was transformed into yWS2542 (ram11 ydj11) using the Frozen-EZ Yeast Transformation II Kit (Zymo Research, Irvine, CA) per the manufacturer’s guidelines ...
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bioRxiv - Microbiology 2024Quote: ... The tissues were homogenized by bead beating and viral RNA was extracted using the RxnDirect-zol RNA Miniprep Plus kit (Zymo). For all samples the viral RNA was quantified by qRT-PCR as described above.
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bioRxiv - Microbiology 2024Quote: ... The size of the PCR products was verified by agarose gel electrophoresis and purified by using the Zyppy extraction kit (Zymo). The amplified PCR products were digested with BamHI and XhoI and ligated with similarly digested pCTX-1-lux ...
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bioRxiv - Physiology 2024Quote: ... The VAT was weighed prior to use and 50 ug of tissue and DNA was extracted using the Quick-DNATM Miniprep Plus Kit (Zymo) following manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2023Quote: ... 400 ng DNA were extracted after MTAC induction and bisulfite-converted using EZ DNA Methylation-Gold Kit (Zymo Research, D5005). PCR amplification was performed to convert ssDNA to dsDNA using primers designed for a region spanning the MATa and the backbone of LacO plasmid used for integration (Table S2) ...
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bioRxiv - Cell Biology 2024Quote: ... when RNA was further purified using the RNA Clean & Concentrator kit with in-column DNase-I treatment (Zymo Research Corp), following manufacturer instructions ...
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bioRxiv - Evolutionary Biology 2024Quote: RNA from the same sample material was extracted using the Quick-RNA Fungal/Bacterial miniprep kit (Zymo Research, CA, USA) using the manufacturer’s protocol and eluted in 25 µl of DNase/RNase free water ...
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bioRxiv - Genetics 2024Quote: ... A single colony was picked and grown in terrific broth (TB) to an optical density (OD) of 0.5.RNA isolation was performed using Direct-zol RNA Miniprep Kit (Zymo Research). RNA was prepared for small RNA sequencing according to the following protocol ...
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bioRxiv - Genetics 2024Quote: ... A cell line was prepared encoding this donor plasmid by transforming BL21(DE3) and making the resultant cell line chemically competent using Mix & Go preparation kit (Zymo). The temperature sensitive donor plasmid was then transformed with a second plasmid encoding a T7-inducible recombinase and a constitutively expressed bridge RNA ...
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bioRxiv - Genomics 2024Quote: ... Eluted DNA was treated with Proteinase K at 55ºC for 30 min and then purified with a ChIP DNA Clean and Concentrator kit (Zymo Research) according to the manufacturer’s instructions.
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bioRxiv - Genomics 2024Quote: ... The recovered rRNA depleted total RNA was further cleaned up and concentrated with the RNA Clean & Concentrator kit (Zymo, R1016).
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bioRxiv - Genomics 2024Quote: Genomic DNA (gDNA) of S1 and S2 was extracted using the Quick-DNA fungal/Bacterial Miniprep Kit (Zymo Research, USA) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: CP-KO RNA was extracted from 15 3h PBF guts with the Direct-zol RNA Mini-prep kit (Zymo Research) including on-column DNase treatment and transcribed into cDNA with the qScript cDNA Synthesis Kit (Quantabio) ...
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bioRxiv - Cancer Biology 2024Quote: ... Genomic DNA was isolated from MV4;11 Cas9 + GeCKOv2 cells collected before and following sustained C6-treatment using the Quick-gDNA MidiPrep Kit (Zymo Research) per manufacturer’s directions ...
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bioRxiv - Genetics 2023Quote: ... For specific miRNA quantification, we size-fractionated small RNAs (< 200nt, containing pre-miRNAs) using RNA Clean & Concentrator 5 column kits (Zymo), as per the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The reactions were cleaned up with RNA Clean and Concentrator 100 columns (Zymo, referred to as “kit-purified” in text). Purity was assessed using a 6% Tris-Borate-Urea gel (Invitrogen).
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bioRxiv - Synthetic Biology 2023Quote: ... treated with 1 μL of DpnI for 3 h at 37℃ and purified with Zymo Research’s Clean & Concentrator-5 kit according to the manufacturer’s instructions (Zymo CN# D4004). Then ...
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bioRxiv - Genetics 2023Quote: ... The PCR products were then pooled at equimolar ratios and cleaned using the DNA Clean and Concentrator Kit (Zymo Research), followed by gel extraction ...
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bioRxiv - Evolutionary Biology 2023Quote: ... and amplicons ranging from 0.2–8 kb were extracted and purified using the Zymoclean Gel DNA Recovery Kit (Zymo Research), quantified using a Qubit 2.0 fluorometer (Life Technologies) ...
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bioRxiv - Microbiology 2023Quote: ... and 10 conventional + Abx individuals was extracted with the Quick-DNA Tissue/Insect Microprep Kit (Zymo Research, Irvine, CA, USA), according to the manufacturer instructions and eluted in 30-40 µL elution buffer ...
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bioRxiv - Cell Biology 2023Quote: MAGIC YKO was constructed with a two-step transformation using the Frozen-EZ Yeast Transformation II Kit (T2001, Zymo Research) following the microscale protocol in 96-well format ...
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bioRxiv - Genomics 2023Quote: ... Each pool of heads or bodies was placed in a well of 96-well plates and total RNA was extracted using Quick-RNA 96 Kit by Zymo Research (Catalog no ...
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bioRxiv - Cancer Biology 2024Quote: ... Total RNA was extracted by phenol/chloroform and purified through the RNA Clean and Concentrator-25 kit (Zymo Research, R1018), following the manufacturer’s instructions ...
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bioRxiv - Synthetic Biology 2024Quote: ... the constructed and corrected recombinant plasmids were digested with Not I for linearization and then transformed into Po1fΔKu70 using the Frozen-EZ yeast transformation II Kit (Zymo Research). Transformants were screened on YNB plates supplemented with auxotrophic supplements containing appropriate amino acids and placed in an incubator at 30 °C for 2-3 day ...
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bioRxiv - Biochemistry 2024Quote: RNA was extracted from various developmental stages and adult tissues using TRIzol Reagent (Thermo Fischer Scientific, Waltham, MA, USA) and further purified with the RNA clean and concentrator-5 kit (Zymo), following the provided instructions ...
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bioRxiv - Molecular Biology 2023Quote: All RNA reporters were made by in vitro transcription with a standard T7 RNA polymerase protocol using DNA template gel extracted using the Zymoclean Gel DNA Recovery Kit (Zymo), 1x T7 RNA Polymerase buffer (NEB) ...
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bioRxiv - Neuroscience 2023Quote: ... RNA was isolated using phenol-chloroform extraction followed by purification with Zymo Direct-zol RNA Miniprep Kit (Zymo Research R2052). Genomic DNA removal and cDNA synthesis were performed with iScript gDNA clear cDNA synthesis kit (Bio-Rad 1725035 ...
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bioRxiv - Immunology 2023Quote: ... was added to the fractions and RNA was isolated using the DIrect-zol RNA Miniprep Plus Kit (Zymo cat# R2052). 16uL of RNA isolated from fractions was reverse transcribed (iScript cDNA synthesis kit ...
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bioRxiv - Genomics 2023Quote: ... 3 colonies were randomly selected and further expanded for plasmid DNA purification (Zyppy™ Plasmid Purification Kit; Zymo research #D4019). Extracted DNA was then sequenced to verify the integrity of the cloned fragments.
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bioRxiv - Molecular Biology 2023Quote: ... Samples were incubated for 30 min at 37°C and purified using the RNA Clean & Concentrator-5 kit (Zymo Research).
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bioRxiv - Bioengineering 2023Quote: hPSCs were lysed with TRI-Reagent and RNA was extracted using the Direct-zol RNA MiniPrep Plus kit (Zymo Research). RNA concentration was measured using a NanoDrop ...
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bioRxiv - Bioengineering 2023Quote: The terminal sorts of the yeast-displayed libraries were miniprepped using the Zymoprep Yeast Plasmid Miniprep Kit (Zymo Research, D2004) to recover enriched yeast plasmids ...
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bioRxiv - Cancer Biology 2023Quote: Tumors were then harvested 4 weeks post-injection and processed using Quick-DNA midiprep plus kit (Zymo Research Cat. #D4075). For each processed tumor ...
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bioRxiv - Neuroscience 2023Quote: ... 500ng of total RNA was used for bisulfite conversion with the EZ RNA Methylation kit according to manufacturer’s instructions (Zymo Research) but with an additional 2 PCR cycles to denature tRNAs completely ...
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bioRxiv - Synthetic Biology 2023Quote: ... coli cells were prepared and transformed with plasmids using the Mix and Go™ kit (Zymo Research, Irvin, CA, USA) according to the manufacturer’s indications ...