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Citations for Thermo Fisher :
4901 - 4950 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2019Quote: ... COS-7 and U2OS cells were grown at 37°C with 5% CO2 in complete DMEM (Thermo Fisher Scientific, USA) containing 10% FBS and 1% L-glutamine ...
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bioRxiv - Plant Biology 2021Quote: ... pistils and young seeds (7 DAP from CT and 5 DAP from HT) were isolated following a protocol for Trizol (Invitrogen). RNA from 26 DAP seeds was isolated using a NucleoSpin RNA Plant and Fungi kit (Macherey-Nagel ...
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bioRxiv - Neuroscience 2022Quote: ... jGCaMP7b-XC or jGCaMP7b-XN and 1 μg cDNA encoding CFP (for labelling the soma area and neurites) were transiently transfected into DIV 5-7 cultured cortical neurons by Lipofectamine 2000 (Invitrogen) with a typical protocol according to the manual ...
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bioRxiv - Neuroscience 2021Quote: ... Brains were extracted from rats aged postnatal day 5-7 and placed in working buffer (Hank’s balanced salt solution (HBSS) (Life Technologies) with 0.5% chromatographically purified bovine serum albumin (BSA ...
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bioRxiv - Biophysics 2022Quote: ... HEK293 and COS-7 cells were maintained at 37 ºC and 5% CO2 and cultured in Dulbecco’s modified Eagle media (Life Technologies) supplemented with 10% FBS (Hyclone ...
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bioRxiv - Molecular Biology 2023Quote: ... Northern blot analysis of U1 snRNA was performed using 5’-radiolabeled DNA probes (Sequence is listed in Supplemental Table 7) in ultrasensitive hybridization Buffer (Thermofisher). The radioactivity signals were analyzed by PhosphorImager (Typhoon FLA 7000).
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bioRxiv - Synthetic Biology 2022Quote: ... 5’ and 3’ RACE ready cDNA was generated from RNA extracted from ~30 pairs of ovaries or testes dissected from 5-7 days-post-emergence (dpe) Liverpool strain adults using Trizol (Invitrogen). Primers LA1076 then nested with LA1352 (5’ ...
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bioRxiv - Microbiology 2024Quote: ... approximately 5 × 105 Huh-7 cells were transfected with 2 μg of purified BAC DNA using Lipofectamine 3,000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Biochemistry 2024Quote: ... Liposomes at 5-7 mM were applied to the carbon side of a grid held in a Vitrobot Mark IV (ThermoFisher) chamber kept at 4°C and 100% relative humidity ...
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bioRxiv - Immunology 2023Quote: Lungs were transferred into 7 ml bijoux tubes containing 500 µl digestion mix consisting of 5 mg/ml collagenase I (Gibco) and 10 µg/ml DNase I (Sigma ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then dissociated using 0.5mM EDTA into a single cell suspension and incubated in 5 μM of cell-permeant 2’,7’-dichlorodihydrofluorescein diacetate (CM-H2DCFDA) dye (Invitrogen) for 30 min at 37°C in the dark ...
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bioRxiv - Microbiology 2023Quote: ... approximately 5×105 Huh-7 cells were transfected with 2 µg of purified BAC DNA using Lipofectamine 3000 (Fisher Scientific) as a transfection reagent ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were passaged every 5-7 days once they reached about 70-80% confluency using TrypLE cell dissociation reagent (Gibco) and gentle scraping before being replated onto new MEFs ...
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bioRxiv - Immunology 2023Quote: ... and washed with HBSS and resuspended in appropriate volume of HBSS pH 7.4 supplemented with 5% FBS before staining with 7-aminoactinomycin D (Life Technologies) to exclude dead cells ...
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bioRxiv - Molecular Biology 2023Quote: ... supplemented with 12.5 ng/mL IL-7 (Miltenyi, USA) and 12.5 ng/mL IL-15 (Miltenyi, USA) or RPMI 1640 (Gibco, USA) medium supplemented with 100 U/mL IL-2 (Sci-Store ...
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bioRxiv - Cell Biology 2023Quote: ... Cyclin B14E7E cells were selected for 72 h with Nutlin-3A 5 µM and for 7 days with Geneticin (Gibco) 0.4 mg/ml ...
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bioRxiv - Cancer Biology 2022Quote: ... in 500 μL HEPES (25 mM)/PBS were mixed overnight at 4 °C with 5 μL of 4 μm aldehyde-activated latex beads (A37304, Invitrogen). The beads were blocked with 200 μL of 1% BSA for 1 h at room temperature ...
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bioRxiv - Biochemistry 2022Quote: ... Supernatant containing P1 virus was harvested ∼4 days post transfection and stored at 4°C after supplementing 5% FBS (Gibco). Expression of the Sec complex was carried out by adding 0.5 mL P1 virus to 0.7 L of Sf9 cells at density of ∼1.5 M/ml that were prepared in a 2-L baffled flask ...
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bioRxiv - Cell Biology 2023Quote: ... with NaOH) and were loaded with 5 μM Ca2+ dye Fluo-4 acetomethyl ester (Fluo-4 AM) (Invitrogen Life Technologies) for 20 minutes at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... with NaOH) and were loaded with 5 μM Ca2+ dye Fluo-4 acetomethyl ester (Fluo-4 AM) (Invitrogen Life Technologies) for 20 minutes at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... pTG-Luc and SARS2-COV2 spike expression vector were transfected into HEK 293T cells at the ratio of 3:4:3 using Lipofectamine 3000 transfection reagent (ThermoFisher Scientific, Waltham, MA). Accession IDs of the spike proteins used in the study were ...
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bioRxiv - Plant Biology 2022Quote: 5’-biotinylated RNA and unmodified RNA of Motif 3 (5’-AAAUCGCCGGAG-3’ 5’-Bio-AAAUCGCCGGAG-3’) were synthesized by Eurofins (Hamburg, Germany) and used for LightShift™ Chemiluminescent RNA EMSA Kit (Thermo Scientific). Total protein was extracted from LL and 10 min LL➔HL-treated plants using extraction buffer (25 mM Tris ...
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bioRxiv - Microbiology 2021Quote: ... the hypervariable V3 region of the 16S rDNA gene was amplified from 20 ng of DNA using the primers 5′-CCTACGGGAGGCAGCAG-3′ and 5′-ATTACCGCGGCTGCTGG-3′ (Integrated DNA Technologies BVBA, Leuven, Belgium),(18) 1U Platinum® PCR SuperMix High Fidelity (ThermoFisher Scientific) and 10 μM of primer-mix ...
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bioRxiv - Neuroscience 2022Quote: ... sections were incubated in 0.05 M Tris-HCl buffer (pH 7.6) containing 5 mg 3-3′ diaminobenzidine (Thermo Scientific, TA-060-HDX) per 10 mL buffer and a final concentration of 0.01% hydrogen peroxide for 15 min at RT ...
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bioRxiv - Immunology 2022Quote: ... and hybridized first using RT primer (5′/5Biosg/AAGCAGTGGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTVN-3′) and then reverse transcribed into cDNA using TSO primer (5′-AAGCAGTGGTATCAACGCAGAGTACATrGrGrG-3’) and RT maxima reverse transcription (Thermo Fisher Scientific). cDNA was amplified using ISPCR primer (5′-AAGCAGTGGTATCAACGCAGAGT-3′ ...
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bioRxiv - Microbiology 2024Quote: ... and a pan VSG reverse primer which binds to a conserved 14bp region of the 3’ UTR (5’-GATTTAGGTGACACTATAGTGTTAAAATATATC-3’) with AmpliTaq Gold (Applied Biosystems, 4398881) (anneal & extension 60C 1m ...
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bioRxiv - Microbiology 2024Quote: ... total RNA was harvested from a 10 ml parasite culture (5% hematocrit; >3% parasitaemia) by resuspending the pelleted RBCs in 3 ml pre-warmed Trizol (Invitrogen #15596-018). After a 5 min incubation step at 37°C ...
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bioRxiv - Immunology 2021Quote: The enumeration of apoptotic cells was performed using the CellEventTM Caspase-3/7 Green Flow Cytometry Assay Kit (catalogue #: C10427; Thermo Fisher Scientific) following manufacturer’s instructions.
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bioRxiv - Cell Biology 2021Quote: ... Human breast carcinoma cell lines (SK-BR-3, MCF-7, MDA-MB-231, T-47D) were cultured in GIBCO® RPMI 1640 (Life technologies) medium supplemented with 10% FBS (Life technologies) ...
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bioRxiv - Bioengineering 2021Quote: ... Cells were passaged on day 3 to maintain sub-confluent culture conditions and analyzed by flow cytometry on day 7 using an Attune NxT (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2022Quote: ... At time of treatment the cells were also treated with 2μM CellEvent caspase-3/7 green detection reagent (C10423; Invitrogen, ThermoFisher Scientific, Inc), which was used to measure apoptosis ...
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bioRxiv - Systems Biology 2023Quote: ... RNA was extracted from 40 pairs of adult testes per sample per condition (3 to 7 days post-eclosion) using the PureLink RNA Mini Kit (Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... the strips were washed 3 times in PBS-T before incubation in primary antibody (GST Tag Mouse anti-Tag, Clone: 8-326, Invitrogen) for 1 h at room temperature ...
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bioRxiv - Genomics 2019Quote: ... The samples were cooled down to RT before loading them on a 3-8% Tris-Acetate Protein Gels (Invitrogen EA0375PK2). Next ...
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bioRxiv - Genetics 2020Quote: ... samples were diluted in Laemmli sample buffer and 2 μg of protein was separated on a NuPage 3–8% Tris-Acetate gel (Invitrogen). The gel was then stained with Coomassie Blue and digitalized.
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bioRxiv - Developmental Biology 2021Quote: ... 20 μg protein lysates were subjected to gel electrophoresis on a 3-8% NuPAGE® Tris-Acetate gel (Life Technologies), and protein was transferred to a Immobilon-FL membrane (Millipore) ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples were boiled for 5min at 95°C before being loaded on a 3-8% NuPAGE Tris-Acetate gel (Invitrogen). Western blots were quantified using ImageJ ...
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bioRxiv - Neuroscience 2020Quote: ... Protein samples were heated to 70°C and loaded on 3-8% Tris-Acetate Protein Gels (ThermoFisher Scientific, Waltham, MA) or NuPAGE 4-12% Bis-Tris Protein Gels (ThermoFisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... Equal amounts of samples (50-100 µg) were resolved by SDS-polyacrylamide gel electrophoresis in 3–8% Tris-Acetate gels (Thermofisher). Gels were wet-transferred in 1x NuPAGE Transfer Buffer (Thermofisher ...
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bioRxiv - Cell Biology 2022Quote: ... 8% Tris-glycine gels at 150 volts for 2.25 hr or pre-poured NuPAGE 3-8% Tris-Acetate gel (Invitrogen; EA03752) for 2.15 hr (Figure 1C ...
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bioRxiv - Pathology 2020Quote: Stored blood samples collected on d 9 from selected 8 NEC and 8 healthy pigs across 3 litters were used for RNA extraction using MagMax 96 blood RNA isolation kit (Thermofisher) for transcriptomics by whole transcriptome shotgun sequencing as previously described24 ...
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bioRxiv - Cell Biology 2021Quote: RBL-2H3 cells in a confluent 75 cm2 flask were washed and trypsinized for 8 min at 37 °C with 3 mL of 0.05% Trypsin-EDTA (Thermo Fisher). The detached cells were resuspended in 7 mL of growth medium and centrifuged to remove the medium ...
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bioRxiv - Molecular Biology 2022Quote: ... quenched in 100 mM Tris-HCl pH 8.0 and analyzed on a NuPAGE™ 3–8 % Tris-Acetate gel (Invitrogen). Fractions containing protein bands corresponding to the expected size of a Pol II–APT complex were dialyzed against 10 mM K-HEPES pH 8.0 ...
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bioRxiv - Microbiology 2022Quote: ... To prepare CONV larvae first instars from surface-sterilized eggs were exposed immediately upon hatching to CONV inoculum at selected dilution (from 10−3 to 10−8) in sterile water (Gibco). Bacterial composition of CONV inoculum is provided (FIG S2 ...
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bioRxiv - Molecular Biology 2022Quote: 20~60μg lysates from culture cells or mouse tissues were separated on 10% Bis-Tris or 3~8% Tris-Acetate Novex NuPAGE gels (Invitrogen) and transferred to nitrocellulose membrane following standard procedures ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: ... Perfusion was subsequently changed to bicarbonate-buffered extracellular solution supplemented with the voltage- sensitive dye Di-8-ANEPPS at 3 µM (D3167, Invitrogen). Following dye loading for 4 minutes ...
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bioRxiv - Developmental Biology 2024Quote: Monolayers and EBs from day 3 to day 8 were dissociated to single cells with TypLE 1X (Thermo Fisher, 12563029) for 5 minutes at 37°C ...
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bioRxiv - Cell Biology 2024Quote: ... and the lysate was subjected to Western blot on NuPAGE Novex 3–8% Tris-acetate gradient gels (Invitrogen™ # EA0375BOX), transferred to a nitrocellulose membrane and blotted against OPA1 (Anti-Mouse ...
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bioRxiv - Microbiology 2023Quote: ... then 5 μg total protein in this buffer was separated by SDS-PAGE on a NuPAGE 3-8% Tris-Acetate Midi Gel (Invitrogen). Protein was transferred to a 0.45 μm PVDF Membrane (Cytiva) ...
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Bio-orthogonal Glycan Imaging of Culture Cells and Whole Animal C. elegans with Expansion MicroscopybioRxiv - Bioengineering 2024Quote: ... were incubated at 37°C for 2 h and analyzed by SDS-PAGE on a 3-8% tris-acete NuPAGE gel (Invitrogen) and stained with Pierce Silver Stain Kit (Thermo Scientific ...