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Citations for Thermo Fisher :
4701 - 4750 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Developmental Biology 2021Quote: ... The sections were counterstained with TO-PRO™-3 (T3605, Invitrogen, OR, USA; dilution 1:1000) and examined using a confocal laser microscope (DMi8 ...
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bioRxiv - Genetics 2019Quote: ... except that 1-Bromo-3-chloropropane was used instead of chloroform and DNAse (TURBO DNase, Ambion) treatment was carried out ...
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control.
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bioRxiv - Genetics 2022Quote: ... The 3-phosphoglycerate kinase (Pgk1) protein was detected using anti-Pgk1 monoclonal antibody (1:30,000; Invitrogen) as a loading control ...
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bioRxiv - Cell Biology 2022Quote: ... pre-complexed for > 20 min with 1 μg DNA and 3 μg lipofectamine 2000 (ThermoFisher 11668019); DNA and lipofectamine were each diluted to 100 μl before combining ...
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bioRxiv - Cell Biology 2022Quote: ... cells were plated and transfected with 1:3 ratio (RNA:Lipofectamine) using Lipofectamine RNAiMAX reagent (ThermoFisher Scientific) following manufacturer recommendations ...
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bioRxiv - Plant Biology 2022Quote: The stock solutions for the GLVs (Z)-3-hexen-1-ol (Z3-HOL, 98%; Acros Organics) and (Z)-3-hexenyl acetate (Z3-HAC ...
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bioRxiv - Immunology 2024Quote: ... and a 3:1 CD3/CD28 mouse T-activator Dynabead to Treg ratio (Thermo Fisher Scientific). On day 3 of expansion ...
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bioRxiv - Cell Biology 2024Quote: ... pre-complexed for > 20 min with 1 μg DNA and 3 μg lipofectamine 2000 (ThermoFisher 11668019); DNA and lipofectamine were each diluted to 100 μl before combining ...
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bioRxiv - Cell Biology 2023Quote: PASMCs were stained with Hoechst 33342 dye (3 μL/ml of 1:100 dilution; 62249; Invitrogen) for 15-30 min at 37° C to visualize apoptotic cells ...
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bioRxiv - Cell Biology 2023Quote: ... with PBS (3 times) and incubated with a secondary antibody (1:500, Molecular Probes AF488 #A11008) and Hoechst33342 (1:2000 ...
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Autoimmune inflammation triggers aberrant astrocytic calcium signaling to impair synaptic plasticitybioRxiv - Neuroscience 2023Quote: ... 1 mM L-cysteine) with papain (3 U/mL) and DNAse I (150 U/μL, Invitrogen) for digestion during 25 min at 37°C ...
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bioRxiv - Plant Biology 2023Quote: ... and 1 µL of template cDNA using a QuantStudio 3 real-tame PCR system (Applied Biosystems). PP2AA3 was used for normalization ...
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bioRxiv - Cancer Biology 2023Quote: ... 3% agarose gels were prepared with a 1:20000 dilution of SYBRSafe DNA gel stain (Invitrogen). Once complete the PCR products were loaded onto the gel and the electrophoresis was run on a Powerpac Electrophoresis system (BioRad ...
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bioRxiv - Cell Biology 2023Quote: ... Samples were then washed and incubated with a 1:1000 dilution of To-Pro-3 (ThermoFisher) in PBS for nuclear staining and then mounted using VectaShield antifade mounting medium (Vector Labs ...
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bioRxiv - Cancer Biology 2023Quote: ... 1% P/S at a cell density between 3 × 105 and 2.5 × 106 cells/ml (GIBCO). Cells were always handled and maintained under sterile conditions (tissue culture hood ...
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bioRxiv - Genetics 2023Quote: ... the nuclei were stained by applying 3 mL of 1:1000 diluted DAPI (Thermo Scientific, 62248) in 2×SSC in 4 minutes ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... + hydrogen peroxidase 3% (1:100, Alexa Fluor 488 Tyramide SuperBoost Kit, goat anti-rabbit IgG, Invitrogen, Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... They were then mounted using culture medium supplemented with 1 μM TO-PRO-3 (Life Technologies) for nucleic acid staining ...
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bioRxiv - Microbiology 2023Quote: O/N bacterial cultures were diluted to an OD600 of 0.15 in TSB with 1 μg/mL 1,1′-didodecyl-3,3,3′,3′-tetramethylindocarbocyanine perchlorate (DiIC12) (Invitrogen). Cultures were grown to an OD600 of 1.0 ...
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bioRxiv - Developmental Biology 2024Quote: ... zebrafish were incubated for 3 h in 1 µM LysoTracker™ Deep Red (Invitrogen™, L12492) in E3 (DMSO) ...
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bioRxiv - Neuroscience 2024Quote: ... or anti-occludin (1:100 in 3% BSA in TBS; Thermo Fisher Scientific, Cat# 71-1500) overnight at 4°C in a humidified atmosphere ...
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bioRxiv - Biochemistry 2024Quote: ... 1-3 nmol of mixed antigen was bound to pre-equilibrated Dynabeads MyOne Streptavidin C1 (ThermoFisher). The purified phages were mixed with the beads pre-loaded with a combination of hCNPase and mCNPase and incubated for 2 h at RT ...
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bioRxiv - Biophysics 2021Quote: ... about ~5 μg of bacmid were transfected using 6 μl of CellfectinII reagent (Thermo Fisher Scientific). 5 days after initial transfection ...
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bioRxiv - Immunology 2020Quote: ... which were labeled with 5-(and-6)-carboxyfluorescein diacetate succinimidyl ester (CFSE; Molecular Probes, Eugene, OR) as described (52) ...
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bioRxiv - Bioengineering 2024Quote: ... SMR frequency peaks were calibrated (hertz to picogram) with 8 μm polystyrene beads with a known density of 1.05 g/mL (Thermo Fisher, Duke Standards). Calibration calculations for both cell volume and buoyant mass were conducted using MATLAB.
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bioRxiv - Immunology 2023Quote: ... The concentration was measured at NanoDrop One/One (Thermo Fisher Scientific) and the quality (RNA integrity number ...
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bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-6 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
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bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-6 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
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bioRxiv - Neuroscience 2022Quote: ... the cerebral cortexes from 2-3 P3-P5 C57BL/6 mice were collected in ice cold HBSS (Invitrogen), the tissue was washed three times with HBSS and digested with 0.04% trypsin (Sigma ...
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bioRxiv - Cell Biology 2022Quote: HCT116 cells were cultured in a 6-well plate at 300,000 cells per well in 3 ml McCoy’s 5A medium (Gibco) supplemented with 10% heat inactivated FBS and with 90 UI/mL Penicillin ...
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bioRxiv - Cell Biology 2023Quote: ... X-biotin-PE [N-((6-(biotinoyl)amino)hexanoyl)-1,2-dihexadecanoylsn-glycero-3-phosphoethanolamine] was obtained from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 30 ovaries from 3-6 day-old flies using TRIzol (Thermo Fisher Scientific) in three replicates ...
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bioRxiv - Molecular Biology 2019Quote: mRNA was purified from 200 µg total RNA extracted from uninfected and infected Huh7 cells (MOI 1, 48 hours post-infection) using one round of polyA selection (Dynabeads mRNA purification kit; Thermo Fisher) and one round of rRNA depletion (NEBNext rRNA depletion kit ...
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bioRxiv - Neuroscience 2021Quote: Freshly dissected retinas were incubated with gentle agitation for one hour at 37°C in 1 mg/mL of pHrodo Red-conjugated zymosan bioparticles (ThermoFisher Scientific) resuspended in retinal culture media (1:1 mixture of DMEM and F-12 supplemented with L-glutamine ...
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bioRxiv - Neuroscience 2019Quote: ... followed by three washes in 0.1%TritonX-100/PBS and one hour of incubation with the secondary antibodies Alexa Fluor 488 and Alexa Fluor 568 (1:500, Life Technologies). Nuclei were counterstained with DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... and then incubated for 2 hours at room temperature in secondary immunoreactive solution containing one or more of the following secondary antibodies: 1:1000 donkey anti-rabbit conjugated to Alexa 488 (Invitrogen A21206) and 1:1000 goat anti-mouse conjugated to Alexa 647 (Invitrogen A21235) ...
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bioRxiv - Immunology 2020Quote: Luciferase-based cytotoxicity assay was carried out for Her2 and CD19 CAR T cells as previously described (22) at a 10:1 ratio of effector to target cells of using Firefly Luc One-Step Glow assay (ThermoFisher #16197). For analysis of cytokine release ...
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bioRxiv - Neuroscience 2020Quote: ... 5 μL of diluted RNA (1:10) was added to 20μL of Superscript III Platinum One-Step qRT-PCR mix (Invitrogen 11746-100) containing 12.5 μL reaction mix ...
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bioRxiv - Microbiology 2020Quote: ... 40 cycles of 15 s at 95 ° C and 1 min at 60 ° C in Step-One-Plus Real-Time PCR System (Applied Biosystems). The standard curve was generated by diluting the DNA of the bacterium Escherichia coli ATCC 25922 in series of 102 – 10−8 (20 – 2 × 109 ng of DNA) ...
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bioRxiv - Microbiology 2020Quote: ... 40 cycles of 15 s at 95 °C and 1 min at 60 °C in Step-One-Plus Real-Time PCR System (Applied Biosystems). The proportion of bacteria in the microbiome was calculated based on the Ct (cycle threshold ...
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bioRxiv - Cell Biology 2021Quote: ... in PBS) and then for one hour in a solution containing phalloidin–Atto647N (1/400 in PBS, Thermo Fisher Scientific, 65906) and DAPI (0.5 μg/ml in PBS ...
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bioRxiv - Immunology 2021Quote: Two different siRNA for thymidylate synthase (TYMS silencer select s14538, s14539) and one control siRNA (silencer select Negative Control #1) (Ambion, Life Technologies) were used at 100 nM ...
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bioRxiv - Genomics 2021Quote: ... We performed one-step multiplex real-time PCR using TaqPathTM 1-Step Multiplex Master Mix (Thermo Fisher Scientific, cat. no. A28526), targeting three different SARS-CoV-2 genes with primer and probe sets specific for Spike (S) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Specimens were washed in PBS and incubated for one hour at room temperature using Texas red goat anti-rabbit antibody (1:1000) (Invitrogen, Ontario). Coverslips were adhered using PureLong Gold mounting medium containing DAPI (Life Technologies) ...
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bioRxiv - Molecular Biology 2022Quote: ... MERS-CoV and REBOV were diluted to 2×10-3 and confirmed by their respective monoplex one-step RT-PCR with specific primers using the Verso 1-step RT-PCR kit (Thermo Scientific). The reaction was set up in 10 μl final volume with 100 nM each of unlabelled forward and reverse primers ...
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bioRxiv - Plant Biology 2022Quote: ... and 2 mL of 1:10 diluted cDNA in an Applied Biosystems Step One Plus real-time PCR instrument (ThermoFisher, USA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... The plates were washed one final time with PBST and were then developed by adding 1-step Ultra TMB (Fisher Scientific) for 60 seconds ...
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bioRxiv - Developmental Biology 2023Quote: ... one day before the start of the experiment (day -1) cells were cultured in media with exosome depleted FBS (Gibco, 15624559). The day 0 ...
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bioRxiv - Neuroscience 2023Quote: ... sections were washed three times with PBS for 20 mins each then incubated for one hour in appropriate fluorescently-conjugated secondary antibodies (1:500, ThermoFisher Australia). Brainstem sections were kept in order and mounted onto gelatin-coated slides ...