Labshake search
Citations for Thermo Fisher :
4951 - 5000 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Biophysics 2023Quote: ... samples were mixed with 2X Novex Tris-glycine native sample buffer and loaded into NuPAGE 3-8% Tris-glycine gels (Invitrogen) with NativeMark unstained protein standard (Thermo) ...
-
bioRxiv - Biophysics 2022Quote: ... CaVβ3-stable cells were grown in suspension at 37°C supplied with 8% CO2 in FreeStyle 293 Expression Medium (Gibco) supplemented with 2% fetal bovine serum (FBS ...
-
bioRxiv - Cell Biology 2023Quote: ... 10-15ug total protein was loaded into wells of 3-8% Tris-Acetate gels and separated in tris acetate buffer (Invitrogen) ran at 65v for 30 minutes and then 125v until the dye front reached the bottom of the gel ...
-
bioRxiv - Molecular Biology 2024Quote: hTERT-RPE1 cells carrying an inducible copy of GFP multimers and FlpIn T-Rex 293 cells carrying an inducible copy of 3×FLAG-SNAP-SAF-A were grown in 8-well Lab-Tek II Chambered Coverglass -- 1.5 borosilicate glass (Thermofisher scientific).
-
bioRxiv - Biochemistry 2021Quote: ... 1 × 106 HEK293T cells were plated in a 6-well dish with 1.5 mL of DMEM growth media (Gibco) containing 10% Fetal Bovine Serum (FBS ...
-
bioRxiv - Genomics 2020Quote: ... 2 µl cDNA (diluted 1:20) was used in a 6 µl Fast SYBR Green qPCR reaction (ThermoFisher Scientific). No-template controls for each gene were run in all qPCR plates ...
-
bioRxiv - Biochemistry 2020Quote: ... according to the manufacturer’s instructions and diluted 1:6 with DNAse/RNAse-free water (Thermo Fisher, Waltham, MA, USA). qRT-PCR was performed with SYBR-Green Master Mix (Thermo Fisher Scientific ...
-
bioRxiv - Bioengineering 2021Quote: ... was dissolved under stirring in 15 mL of an alginate solution (10 mg/mL-1) formed in 0.1 M MES buffer at pH 6 (ThermoFisher). Sulfo-NHS (41 mg ...
-
bioRxiv - Cell Biology 2021Quote: ... the cells were seeded at low and high density in 6-or 12-well plates coated with 0,2% type 1 Collagen (Gibco) and grown in PM for 24 hours ...
-
bioRxiv - Cancer Biology 2021Quote: ... NALM-6 cells were cultured in RPMI media supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco). MCF7 cells were cultured in DMEM media supplemented with 10% fetal bovine serum and 1% penicillin/streptomycin (Gibco) ...
-
bioRxiv - Molecular Biology 2021Quote: ... 293T cells were cultured in Dulbecco’s Modified Eagle’s Medium (DMEM) with 6% fetal bovine serum (FBS) and 1% Antibiotic-Antimycotic (Gibco, 15240062). T cell lines were cultured in Roswell Park Memorial Institute (RPMI ...
-
bioRxiv - Immunology 2020Quote: 250 μL K92 peptide at 1 mg/mL was reduced with 6 μL of 0.5 M Dithiothreitol (Thermo Fisher) for 40 minutes at 37 °C and alkylated with addition of 18 μL of 2-Iodoacetamide (Thermo Fisher) ...
-
bioRxiv - Microbiology 2022Quote: ... 60 °C for 1 min) X 49 cycles on the QuantStudio 6 flex Real-time PCR system (Applied Biosystems), using the TaqMan RNA-to-CT 1-step kit (Applied Biosystems).
-
bioRxiv - Immunology 2023Quote: ... and rested for ∼6 hours in R15 (RPMI 1640 medium supplemented with 15% FBS, 1% antibiotic-antimycotic [Thermo Fisher Scientific] ...
-
bioRxiv - Biophysics 2024Quote: ... at a 6:1:2 ratio for each virus using the Bac-to-Bac baculovirus expression system (Thermo Fisher). Cell cultures were grown in ESF 921 medium (Expression Systems ...
-
bioRxiv - Physiology 2024Quote: ... Cells were grown overnight in glass slides and loaded with 10-6 mol.L-1 4,5-diaminofluorescein-diacetate (DAF-2DA; Molecular Probes) for 30 minutes ...
-
bioRxiv - Pathology 2024Quote: ... IRB number: VGHUST105-G7-6-1) were cultivated in Dulbecco’s modified Eagle’s medium (Thermo Fisher Scientific, Waltham, MA, USA) supplemented with 10% heat-inactivated fetal bovine serum ...
-
bioRxiv - Developmental Biology 2021Quote: ... re-suspended in PBS containing a viability marker (7-AAD – 7-Aminoactinomycin, Invitrogen – A1310), filtered again through 70-µm cell strainers and incubated on ice for 5 minutes ...
-
bioRxiv - Developmental Biology 2022Quote: ... 7 and 7 fish respectively and dissociated in 0.25% Trypsin-EDTA (Gibco, ThermoFisher Scientific) and 8 mg/ml Collagenase from Clostridum histolyticum (Sigma-Aldrich ...
-
bioRxiv - Developmental Biology 2022Quote: ... 7 and 7 fish respectively and dissociated in 0.25% Trypsin-EDTA (Gibco, ThermoFisher Scientific) and 8 mg/ml Collagenase from Clostridum histolyticum (Sigma-Aldrich ...
-
bioRxiv - Synthetic Biology 2023Quote: ... The samples were incubated overnight with Paired Box 7 (PAX 7) antibodies (Invitrogen, USA) in blocking solution at 4°C ...
-
bioRxiv - Pharmacology and Toxicology 2020Quote: ... an interleukin-8 (IL-8) ELISA kit (Invitrogen, Catalog # CHC1303) to quantify secreted IL-8 levels in conditioned media ...
-
bioRxiv - Plant Biology 2022Quote: Replicate trials of soil grown grafted plants from 3-7 days post-grafting were cut at their root tips and dipped into (5 mg/mL) of 5(-and-6)-Carboxyfluorescein Diacetate (CFDA) (Invitrogen®, Waltham, MA, USA). Plants were incubated under full-spectrum LED lights (Cirrus LED Grow Lights ...
-
bioRxiv - Immunology 2022Quote: About 80,000 MCF-7 cells were placed in each chamber of a 4-well chamber slide (Thermo Scientific, cat. no. 177399) containing 500 μl of Dulbecco’s Modified Eagle Medium (DMEM ...
-
bioRxiv - Microbiology 2020Quote: The in vitro enzymatic assay with the fluorogenic substrate ubiquitin-7-amido-4-methylcoumarin (Ub-AMC, UbiQ) was assembled in a 384-well microplate (Thermo Scientific). Assays were performed in 20 μL reaction volumes and in triplicate ...
-
bioRxiv - Immunology 2020Quote: Macrophages were incubated with the fluorescent glucose analog 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG) (10 μM, Invitrogen, California, USA) in PBS for 30 min ...
-
bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt™ 4-12% Bis-Tris Plus gels or 7% Tris-Acetate gels (Invitrogen, Thermo Fisher Scientific), blotted onto a nitrocellulose membrane (926-31092 ...
-
bioRxiv - Physiology 2021Quote: ... Proteins were separated on Bolt™ 4-12% Bis-Tris Plus gels or 7% Tris-Acetate gels (Invitrogen, Thermo Fisher Scientific), blotted onto a nitrocellulose membrane (926-31092 ...
-
bioRxiv - Immunology 2021Quote: ... containing 2’-(N-(7-nitrobenz-2-oxa-1,3-diazol-4-yl)amino)-2-deoxyglucose (2-NBDG; 50 μM; Thermo Fisher Scientific). To determine FA uptake ...
-
bioRxiv - Microbiology 2022Quote: ... with 250ng each of the 8 plasmids corresponding with each segment of viral genome (or 7 plasmids for mock) and 4 µl Lipofectamine 2000 transfection reagent (Thermo Fisher) per well ...
-
bioRxiv - Cell Biology 2024Quote: ... Glucose was traced using 2-(N-(7-Nitrobenz-2-oxa-1,3-diazol-4-yl)Amino)-2-Deoxyglucose (2-NBDG, Invitrogen, ThermoFisher, cat. #N13195) combined with 0,57 mg/mL 40kDa tetramethyl-rhodamine Dextran (Invitrogen ...
-
bioRxiv - Biochemistry 2023Quote: ... Samples were boiled at 99 °C with shaking for 7 min and 25 μg of protein per sample was analysed with 4-12% Bis-Tris gels (Life Technologies) according to manufacturer’s instructions ...
-
bioRxiv - Cell Biology 2024Quote: ... Samples were then dried at 100°C and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
-
bioRxiv - Cell Biology 2024Quote: ... The flow-through was then dried in a speed-vac and derivatized with 7-chloro-4-nitrobenzo-2-oxa-1,3-diazole (Acros Organics, ThermoFisher Scientific) prior to reverse-phase HPLC (Agilent 1100 series ...
-
bioRxiv - Molecular Biology 2023Quote: ... 20 μl was loaded on to NuPAGE 4 to 12% Protein Gels with 7 μl of 4X NuPAGE LDS sample buffer as described by the manufacturer (Invitrogen: NP0322BOX) and run for 2 h at 100 V ...
-
bioRxiv - Neuroscience 2023Quote: Third instar larval brains were dissected in phosphate buffered saline (PBS) and fixed with 4 % formaldehyde (FA) pH 7 (Thermo Scientific)/PBS for 20 minutes at room temperature ...
-
bioRxiv - Neuroscience 2023Quote: ... 7-10 μg (cultured astrocyte lysates) of protein was loaded into Bolt™ 4–12% Bus-Tris Plus gels (Thermo Scientific) and run at 150 V for 1 hour ...
-
bioRxiv - Molecular Biology 2023Quote: ... Movies were collected on a Titan Krios G3i at spot size 7 with a Falcon 4 Direct Detector (Thermo Fisher Scientific) equipped with an energy filter set to 20 eV ...
-
bioRxiv - Developmental Biology 2022Quote: ... NCC primary cultures were performed in 4-well plates or in 8-well Chambered Coverglass (Nunc, cat. no. 154511) coated with fibronectin ...
-
bioRxiv - Cell Biology 2023Quote: ... 8 µg of protein was separated on a NuPAGE 4–12% Bis-Tris gel (Life technologies, Carlsbad, CA, USA). Proteins were electroblotted to 0.45 µm PVDF membranes (Imobilon-P ...
-
bioRxiv - Biochemistry 2023Quote: ... Handcast SDS-PAGE gels (8%, 10%, 15%, or 4-18% gradient) containing 0.5% (v/v) trichloroethanol (Acros Organics 139441000) for stain-free imaging (79 ...
-
bioRxiv - Cell Biology 2020Quote: ... We cultured cells in customized media consisting of low-glucose (1 g/L) DMEM with phenol red (Gibco, USA), 1 g/L sodium bicarbonate ...
-
bioRxiv - Cell Biology 2020Quote: ... Cells (at least ~1 g starting material) grown in shaking culture (in glucose-containing RPMI as described above, with 0.1% pluronic (Gibco)) were resuspended in low tonicity buffer (100 mM sucrose ...
-
bioRxiv - Cell Biology 2020Quote: ... Secondary antibodies (Alexa Fluor 488, 594-conjugated goat anti-rabbit or anti-mouse immunoglobulins G (IgGs) (1/1,000; Invitrogen) at 37°C for 30 min ...
-
bioRxiv - Molecular Biology 2020Quote: ... Each half of diluted lysate is incubated with 1/10 volume of antibody coated Protein G Dynabeads™ (Invitrogen) for 40 min at 4°C by end to end rotation ...
-
bioRxiv - Molecular Biology 2021Quote: ... 293T and mouse hepatoma (Hepa1c1c) cells were cultured in DMEM (4.5 g l-1 glucose, Hyclone) supplemented with 10% FBS (Gibco) and 100 units (penicillin) ...
-
bioRxiv - Physiology 2019Quote: ... Cells were pelleted by spinning at 5000 x g and pellet was resuspended in 1 ml of OptiMEM (Gibco). 100 μl of resuspended cells and 2-4 μg of desired plasmid DNA in 5 μL of volume were pipetted into electroporation cuvettes (2 mm gap ...
-
bioRxiv - Cell Biology 2019Quote: ... Extracts were clarified using two rounds of centrifugation (20,000 X g, 20 min) and then bound to 1 ml of Ni-NTA agarose beads (Invitrogen) in lysis buffer containing 5mM imidazole for 2-4 hr ...
-
bioRxiv - Genomics 2020Quote: ... Pooled fractions were tumbled at 4°C for 1 hour with 5μg of antibody (Key Resources Table) and 25μL Dynabeads™ Protein A or Protein G (ThermoFisher Scientific ...
-
bioRxiv - Cell Biology 2019Quote: ... Anti-PRG-1 antibody (Custom) or rabbit IgG was pre-coupled to protein A/G matrix (Thermo Scientific, 88802) and incubated with the supernatant of worm lysates for 4 h (Anti-PRG-1 with N2 lysates ...