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Citations for Thermo Fisher :
5101 - 5150 of 10000+ citations for 6H 1 3 Dioxolo 4 5 g 1 benzopyran 6 one 7 8 dihydro since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Solubilized membranes were mixed 1:1 with binding buffer (20 mM Tris pH 8/1 M NaCl/20 mM imidazole) and loaded onto a HisPur Ni-NTA (Thermo Scientific) column equilibrated with 20 mM Tris pH 8/750 mM sucrose/500 mM NaCl/10 mM imidazole/0.2% DDM (equilibration buffer) ...
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bioRxiv - Biochemistry 2020Quote: ... High-resolution mass spectra and MS/MS fragmentation pattern of 1-8 were recorded using a Q Exactive Plus mass spectrometer (Thermo Scientific). Chromatography for determination of amino acyl hydroxamate was performed on a Waters ACQUITY H-class UPLC system coupled to a Xevo TQ-S micro (Waters ...
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bioRxiv - Molecular Biology 2021Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Immunology 2020Quote: ... was applied to the grids and blotted (Whatman #1 paper) for 2 to 8 s using Vitrobot Mark IV (Thermo Fisher) with a blot force of 5 ...
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bioRxiv - Cell Biology 2022Quote: ... and 4.8 µL cDNA (diluted 1:20 prior to use) was added to each well of qPCR plates (REF 4346906, Life Technologies). Samples were run on a StepOnePlus™ RT-qPCR machine (Applied Biosystems) ...
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bioRxiv - Molecular Biology 2022Quote: Total 40μg of protein from cell lysates were loaded in 8%SDS-PAGE gel and immunoblotted with RAD51 antibody (1:1000; PA5-27195; Thermofisher Scientific) diluted in 1%BSA+TBS+ 0.1%Tween 20 ...
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bioRxiv - Neuroscience 2022Quote: ... Differentiated iNeurons were then cryogenized as aliquots of 1 · 106 to 8 · 106 cells in heat inactivated fetal bovine serum (FBS, 10270-106, Thermo Fisher) containing 5% v/v dimethyl sulfoxide (DMSO) ...
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bioRxiv - Immunology 2024Quote: ... cleanup beads were used (0.8:1 beads/sample ratio) and then eluted in 10 µL of DNase/RNase-Free Distilled Water (10977- 049, ThermoFisher®). cDNA was quantified by the Quant-iT PicoGreen dsDNA Assay Kit (P7581 ...
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bioRxiv - Neuroscience 2023Quote: ... were maintained in DMEM with 1g/L of D-glucose supplemented with 8% Fetal Bovine Serum/FBS and 1% Pen/Strip (Thermo Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Imaging was done in 8-well glass-bottomed dishes (Lab-Tek Chambered #1 Borosilicate Coverglass System 155411, Thermo Scientific Nunc, USA).
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bioRxiv - Genetics 2023Quote: ... IP and input DNA were purified using ChIP elution buffer (250 mM NaCl, 1% SDS, 10 mM Tris-Cl pH 8, 1mM EDTA) and incubation with proteinase K (ThermoFisher Scientific) overnight at 65°C ...
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bioRxiv - Genomics 2023Quote: ... pombe cells were mixed in an 8:1 ratio and total RNA was extracted using the RiboPure yeast kit (Ambion, Life Technologies) using the following volumes ...
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bioRxiv - Developmental Biology 2023Quote: ... Imaging was done in 8-well glass-bottomed dishes (Lab-Tek Chambered #1 Borosilicate Coverglass System 155411, Thermo Scientific Nunc, USA).
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bioRxiv - Biochemistry 2023Quote: ... Freshly isolated mitochondria were resuspended in 50 μl of click-it lysis buffer (50 mM TRIS-HCl pH 8, 1% SDS, 250 U/mL Universal Nuclease [Thermo Fisher]) and incubated on ice for 15 min ...
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bioRxiv - Cell Biology 2024Quote: ... Click-it reaction was performed after staining with primary antibodies by incubating cells in Click-it buffer (100 mM Tris-HCl pH 8, 2 mM CuSO4, 1 ng Alexa Fluor 647 Azide (Life Technologies), and 100 mM ascorbic acid for 30 min at room temperature (RT) ...
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bioRxiv - Microbiology 2022Quote: ... Cells were seeded at a density of 106 cells/well or 8×105 cells/well in a 6-well or 96-well tissue culture plate (Nunc; Thermo Fisher Scientific), respectively ...
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bioRxiv - Immunology 2023Quote: ... the debris spot was generated by adding 6 μL of the solution containing the necrotic hepatocytes into an 8-well chamber slide (Nunc, Rochester, NY, USA) and drying for 2 hours in the laminar flow ...
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bioRxiv - Immunology 2023Quote: ... We isolated CD4 T cells from pooled spleen and lymph nodes of 6-8-week-old Foxp3IRES-GFP mice using magnetic negative selection using the Dynabeads Untouched Mouse CD4 Cells Kit (ThermoFisher Scientific, cat#11415D), resting cells for at least 30 min at 4°C prior to electroporation ...
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bioRxiv - Microbiology 2023Quote: ... Cells were seeded at a density of 106 cells/well or 8×105 cells/well in a 6-well or 96-well tissue culture plate (Nunc; Thermo Fisher Scientific), respectively ...
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bioRxiv - Cell Biology 2022Quote: ... at 37°C in a 5% CO2 incubator with daily media changes and were passaged every 4-5 days using TrypLETM (ThermoFisher), following manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2019Quote: ... The samples were then rinsed with PBS (5 × 3 min) and incubated for 1 h at 37°C with the following secondary antibodies: anti-human Alexa 488 (Thermo Fisher, Waltham, MA, USA; 1:750) or anti-mouse Alexa 488 (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... recombinant human IL-3 (R&D, Cat. 203-IL-050/CF, 25 ng mL-1) or c) human M-CSF recombinant protein (Invitrogen, Cat. PHC9501, 100 ng mL-1). A partial medium change was performed every 48 hours with 2x cytokine composites to replenish cytokines ...
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bioRxiv - Animal Behavior and Cognition 2023Quote: ... After the secondary antibody another four 15min washes in PBST (here 0.5% tween) with 1% BSA were performed followed by a short 5min incubation in TO-PRO™-3 Iodide (642/661)(Invitrogen, cat# T3605, 1:1000 dilution). The animals were mounted in moviol with DAPCO on glass slides and stored at 4°C till imaging.
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bioRxiv - Cell Biology 2021Quote: ... and one-fifth of the affinity purification (IP) were separated on Bolt 4-12% Bis-Tris Plus 15-well gels (Invitrogen), run in Bolt 1x MOPS SDS running buffer (Novex ...
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bioRxiv - Microbiology 2021Quote: ... Genomic DNA was eliminated from RNA extracts by digesting for one hour at 37°C with 4 U of TURBO DNAase (Ambion) followed by purification with MEGAclear Transcription Clean-Up kits (Ambion).
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bioRxiv - Microbiology 2021Quote: ... Protein purity was determined by resolving one to two micrograms of purified protein by SDS-PAGE using NuPAGE 4–12 % Bis Tris precast gels (ThermoFisher) for 50 minutes at 200 V ...
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bioRxiv - Genomics 2022Quote: ... We estimated DNA quality using a NanoDrop One Microvolume UV-Vis Spectrophotometer and quantified DNA using a Qubit 4 Fluorometer (ThermoFisher) with a Quant-iT dsDNA HS Assay Kit ...
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bioRxiv - Microbiology 2022Quote: ... Protein purity was determined by resolving one to two micrograms of purified protein by SDS-PAGE using NuPAGE 4–12% Bis Tris precast gels (ThermoFisher) for 50 minutes at 200V ...
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bioRxiv - Cell Biology 2019Quote: ... Mixed protein eluates were separated by one-dimensional gel electrophoresis on a 4–12% NuPage Novex Bis–Tris gel (Invitrogen), stained with the Colloidal Blue Staining Kit (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... One to 20 μg of total protein were separated by NuPAGE™ 4-12% Bis-Tris Gel (Thermo Fisher Scientific) and transferred onto PVDF membranes (Bio-Rad ...
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bioRxiv - Developmental Biology 2021Quote: ... were incubated for 4 h in DMEM/F12 containing 5% horse serum (Gibco) and 5% foetal bovine serum (Gibco ...
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bioRxiv - Molecular Biology 2021Quote: ... Total RNA (5 μg) was separated in 4-20% TBE gel (ThermoFisher scientific), described above.
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bioRxiv - Microbiology 2021Quote: ... 14.5 μl of preheated reaction mixture [4 μl First Strand buffer (5 ×, Invitrogen), 1 μl 0.1 M dithiothreitol ...
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bioRxiv - Microbiology 2020Quote: ... HIOs were cultured in groups of 5/well using 4-well plates (ThermoFisher). Individual HIO lumens were microinjected using a glass caliber needle with 1μl of PBS control or different STm mutants (105CFU/HIO or 103CFU/HIO for 24h infections) ...
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bioRxiv - Pathology 2021Quote: ... washed platelets were stained with Fluo-4 AM (5 μM, Thermo Fisher Scientific) for 30 min at 37°C ...
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bioRxiv - Systems Biology 2022Quote: ... the reaction was quenched with 4 μl of 5% hydroxylamine (ThermoFisher Scientific, 90115) for 15 min ...
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bioRxiv - Microbiology 2023Quote: ... which included 5 µL 4× Taqman Fast Advanced Master Mix (Thermo Fisher Scientific), 0.4 µL of each primer (tat 2.0 and rev ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were loaded with 5 μM of Fluo-4-AM (Thermo Fisher Scientific) for 50 min at room temperature in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... 5% CO2 for 4 minutes and resuspended in E8 medium (Thermo Fisher Scientific) and 10 μM Y-27632 Rho-kinase inhibitor (ROCKi ...
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bioRxiv - Bioengineering 2023Quote: ... calcium imaging was performed using 5 μM Fluo-4-AM (ThermoFisher, F14201, US) in Krebs-Ringer’s solution containing NaCl 119 mM ...
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bioRxiv - Genomics 2023Quote: ... hiPSC-CMs were loaded with Fluo-4-acetoxymethyl (AM)-ester (5 μM, Invitrogen) in Tyrode’s buffer (135 mM NaCl ...
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bioRxiv - Genomics 2020Quote: ... Abl.3 and Abl.4 (13) were cultured in Roswell Park Memorial Institute medium (Gibco), containing 15% FBS (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: HAEC cells (passage 3-4) were lysed in ice-cold RIPA buffer (ThermoFisher, cat# 89900) containing protease and phosphatase inhibitors (ThermoFisher ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell number was determined every 3-4 days using the Countess automated cell counter (Invitrogen). 20,000 cells were then re-plated with fresh media and compound ...
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bioRxiv - Neuroscience 2019Quote: ... Lines were passaged every 3-4 days using enzymatic detachment with Stempro Accutase (ThermoFisher; A1110501) for 5 minutes and re-plated in mTeSR1 medium with 10µM Rock Inhibitor (Y2732 ...
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bioRxiv - Biochemistry 2021Quote: ... RNA was isolated from approximately 3-4 x 107 cells using TRIzol reagent (Thermo Fisher), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... for 3-4 hr in the presence of brefeldin A (BFA; 10ug/mL; Life Technologies).
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bioRxiv - Neuroscience 2022Quote: Hippocampal neurons were transfected at day in vitro (DIV)3-4 using Lipofectamine 2000 (Invitrogen). Shortly ...
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Migration and establishment of progenitor pool of melanocytes is governed by SEMA3E-PLXND1 signalingbioRxiv - Developmental Biology 2023Quote: ... cells were switched to M254 medium for 3-4 population doublings (Thermofisher Scientific, Life Technologies).
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Migration and establishment of progenitor pool of melanocytes is governed by SEMA3E-PLXND1 signalingbioRxiv - Developmental Biology 2023Quote: ... cells were switched to M254 medium for 3-4 population doublings (Thermofisher Scientific, Life Technologies).