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Citations for Thermo Fisher :
4651 - 4700 of 5129 citations for MRPL54 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2019Quote: Effector candidates without their signal peptide were cloned into the pEarleyGate100 (pEG100) vector for plant expression [78] using Gateway cloning (Thermo Fisher Scientific). Candidate effector genes were amplified from genomic DNA isolated from spores of B ...
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bioRxiv - Cancer Biology 2020Quote: Peptides were analyzed on a Q Exactive HF interfaced with an Ultimate™ 3000 RSLCnano System (Thermo Fisher Scientific, San Jose, CA). The dried peptides and the enriched phosphopeptides were loaded onto a nano-trap column (Acclaim PepMap100 Nano Trap Column ...
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bioRxiv - Physiology 2021Quote: ... The stained bands were excised and processed for in-gel tryptic digestion (Shevchenko et al., 2006) and the eluted peptides were then analyzed using a Thermo Orbitrap Fusion Lumos Tribrid (Thermo Scientific™) mass spectrometer in data-dependent acquisition mode ...
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bioRxiv - Microbiology 2020Quote: LC-MS/MS analysis of tryptic peptides was performed on an Ultimate 3000 RSLC nano instrument coupled to a QExactive HF mass spectrometer (both Thermo Fisher Scientific). Tryptic peptides were trapped for 4 min on an Acclaim Pep Map 100 column (2 cm × 75 μm ...
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bioRxiv - Microbiology 2021Quote: ... Fractionated peptides (6 fractions in triplicate, total 18 runs) were analyzed on Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) interfaced with Easy-nLC 1000 nanoflow liquid chromatography system (Thermo Scientific ...
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bioRxiv - Developmental Biology 2020Quote: ... Peptides were loaded onto a trap column (PepMap C18, 5 mm × 300 μm ID, 5 μm particles, 100 Å, Thermo Fisher Scientific) at a flow rate of 25 μL min−1 using 0.1% TFA as mobile phase ...
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bioRxiv - Immunology 2020Quote: ... FP8-GGGC peptides were conjugated to Qβ-VLPs using the bifunctional cross-linker succinimidyl 6-[(β-maleimidopropionamido) hexanoate] (SMPH; Thermo Fisher Scientific) as described previously for the conjugation of amyloid-beta (Aβ ...
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bioRxiv - Cancer Biology 2019Quote: LC-MS/MS analysis of iTRAQ-labeled tryptic peptide fractions was performed using an LTQ-Orbitrap Velos (Thermo Fisher Scientific, Waltham, MA). Tryptic peptides were separated prior to analysis using a Dionex Ultimate 3000 RSL C nanosystem (Thermo Fisher Scientific ...
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bioRxiv - Systems Biology 2019Quote: ... equivalent aliquots of the digested peptides were analyzed in triplicate with an Easy-nLC 1200 coupled to a Q Exactive HF mass spectrometer (Thermo Fisher Scientific). A 20 x 0.1 mm i.d ...
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bioRxiv - Physiology 2019Quote: ... Inclusion bodies of the antigen peptides were harvested from the transformant Escherichia coli BL21 Star (DE3) (Thermo Fisher Scientific, Waltham, MA, USA) (37) ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides were subjected to ESI source followed by tandem mass spectrometry (MS/MS) coupled online to the UHPLC (Thermo Fisher Scientific). Intact peptides were detected in the Orbitrap at a resolution of 60,000 ...
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bioRxiv - Biochemistry 2021Quote: Dansyl-derivatized or unmodified peptides after tryptic or chymotryptic digest were analyzed via LC- MS/MS with an LTQ Orbitrap Elite (Thermo Fisher Scientific) as follows ...
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Disruption of the TCA cycle reveals an ATF4-dependent integration of redox and amino acid metabolismbioRxiv - Biochemistry 2021Quote: The tryptic peptides were analyzed on a Fusion Lumos mass spectrometer connected to an Ultimate Ultra3000 chromatography system (both Thermo Scientific, Germany) incorporating an autosampler ...
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bioRxiv - Cell Biology 2021Quote: ... Peptides were separated by a PepMapTM RSLC C18 EasySpray column (2 µm, 100Å, 75 µm x 50 cm) (Thermo Fisher Scientific, UK), operating at 50°C ...
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bioRxiv - Immunology 2020Quote: ... peptide separation using acetonitrile gradient was run for 120 min and (ii) data was collected on an Orbitrap Fusion (Thermo Fisher Scientific) operated at 120,000 resolution using HCD (higher-energy collisional dissociation ...
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bioRxiv - Biochemistry 2021Quote: ... 2 μL of each sample were subjected to reverse phase liquid chromatography for peptide separation using an RSLCnano Ultimate 3000 system (Thermo Fisher Scientific). Therefore ...
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bioRxiv - Biochemistry 2021Quote: ... These peptide mixtures were desalted using Oasis HLB cartridges (Waters, Milford, MA) and dried by vacuum evaporation (Thermo Fisher Scientific, Bremen, Germany). The digested peptides (n=30 ...
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bioRxiv - Cell Biology 2020Quote: ... Titanium dioxide-enriched peptides were introduced via an EASY-Spray nano-electrospray ion source into an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific). Other samples were injected into Q-exactive mass spectrometers (Thermo Fischer Scientific) ...
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The derlin Dfm1 promotes retrotranslocation of folded protein domains from the endoplasmic reticulumbioRxiv - Cell Biology 2020Quote: ... peptides were separated by nano-flow reversed-phase liquid chromatography coupled to Q Exactive Hybrid Quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific). Peptides were loaded on a C18 PepMap100 pre-column (inner diameter 300μm×5mm ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were separated on reversed phase C18 columns (precolumn: Acclaim PepMap 100, 300 μm × 5 mm, 5μm, 100 Å (Thermo Fisher Scientific); separation column ...
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bioRxiv - Biochemistry 2020Quote: ... Peptides were reconstituted in LC-MS injection buffer (3% acetonitrile, 0.1% formic acid) and quantified by Pierce Quantitative Fluorometric Peptide Assay (Thermo Scientific, Product #23290).
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bioRxiv - Evolutionary Biology 2020Quote: ... 3 μl of each sample were subjected to reverse phase liquid chromatography for peptide separation using an RSLCnano Ultimate 3000 system (Thermo Fisher Scientific). Therefore ...
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bioRxiv - Evolutionary Biology 2020Quote: ... peptides were loaded on an Acclaim PepMap 100 pre-column (100 μm × 2 cm, C18, 5 μm, 100 Å; Thermo Fisher Scientific) with 0.07% trifluoroacetic acid at a flow rate of 20 μL/min for 3 min ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were analysed by online nanoliquid chromatography coupled to tandem mass spectrometry (UltiMate 3000 and LTQ-Orbitrap Velos Pro (Thermo Fisher Scientific) for the CPSF1 ...
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bioRxiv - Microbiology 2020Quote: ... Peptides were reconstituted in 0.5 M TEAB and processed following the instruction manual for the TMT kit (Thermo Fisher Scientific, Waltham, USA). The tryptic peptides were fractionated by high pH reverse-phase HPLC using a Thermo Betasil C18 column (5 μm particles ...
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bioRxiv - Microbiology 2020Quote: ... before peptide separation on a nanoscale PepMap 100 C18 nanoLC column (3 μm, 100 Å, 75 μm i.d. × 50 cm, Thermo Fisher Scientific) at a flow rate of 0.2 μL per min using a 120 min gradient comprising 100 min from 4% to 25% of solvent B and 20 min from 25% to 40% of solvent B (solvent A consisted in 0.1% formic acid in water ...
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bioRxiv - Molecular Biology 2021Quote: ... analysis of peptide fractions was carried out on an EASY-nLC 1000 coupled to an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific). For each fraction ...
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bioRxiv - Immunology 2020Quote: ... assays [17] were generated from a mixture containing 100 fmol of each heavy reference peptide and shotgun data-dependent acquisition (DDA) LC-MS/MS analysis on a Thermo Orbitrap Fusion Lumos platform (Thermo Fisher Scientific). The setup of the μRPLC-MS system was as described previously [18] ...
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bioRxiv - Immunology 2021Quote: ... Protein identification by liquid chromatography tandem mass spectrometry (LC-MS/MS) analysis of peptides was performed using an LTQ Orbitrap Velos MS (Thermo Fisher Scientific) interfaced with a nanoAcquity LC system (Waters ...
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bioRxiv - Immunology 2020Quote: ... 1 µg of digested peptides were loaded into mass spectrometry system (Q Exactive Hybrid Quadrupole-Orbitrap MS manufactured by ThermoFisher Scientific, USA). For separation of peptides Hypersil gold C18 (100x2.1 mm ...
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bioRxiv - Immunology 2020Quote: ... One population was pulsed with 50 μM of a relevant peptide and labeled with a high concentration (2.5 μM) of carboxyfluorescein diacetate succinimidyl ester (CFSE) (Molecular Probes, Eugene, OR). The other population was unpulsed and labeled with a lower concentration (0.25 μM ...
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bioRxiv - Cell Biology 2021Quote: ... Eluted peptides were introduced directly via a nanospray ion source into a Q Exactive Plus hybrid quardrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific). The mass spectrometer was operated in standard data dependent mode ...
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bioRxiv - Cell Biology 2021Quote: ... The peptides were separated on an EASY Spray C18 column (50 cm x 75 cm, 2 µm particle size) (ThermoFisher Scientific, Toronto) using an EASY nLC-1200 ...
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bioRxiv - Biochemistry 2021Quote: SARS-CoV-2 RBD proteins for SPR binding assays (residues 328-531 of S protein from GenBank NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Cancer Biology 2020Quote: ... Peptide mixture concentration was estimated by measuring absorbance at 280 nm with a NanoDrop 2000 spectrophotometer (Thermo Scientific, San Jose, CA, USA) and a standard curve made from MassPREP E ...
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bioRxiv - Biochemistry 2022Quote: ... The amount of peptide material in each resuspended sample was approximately quantified with a NanoDrop OneC microvolume UV-Vis spectrophotometer (Thermo Fisher Scientific), and typically ∼1 µg of the peptide was injected onto the LC-MS system for each run ...
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bioRxiv - Cancer Biology 2022Quote: ... Peptides were first trapped on a C18 precolumn (Acclaim PepMap 100, 300 μm × 5 mm, 5 μm, 100 Å) (Thermo Fisher Scientific) and eluted peptides were subsequently separated on a μPAC 50 column (PharmaFluidics ...
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bioRxiv - Cancer Biology 2022Quote: ... acetonitrile 0.1% (v/v) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (ThermoFisher Scientific, Inc) over a 150 min organic gradient ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting peptides were analyzed with a nanoAcquity UPLC system (Waters) coupled to an Orbitrap Q Exactive HF mass spectrometer (Thermo Fisher Scientific) employing the LC-MS/MS protocol in a data-independent acquisition mode ...
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bioRxiv - Biochemistry 2022Quote: ... The peptides were passed through Oasis HLB cartridges (Waters, USA) for desalting and then dried by vacuum evaporation (Thermo Fisher Scientific, Germany).
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bioRxiv - Biochemistry 2022Quote: ... Mixed peptide samples were prepared from 9 μL of each sample and separated using a high pH reversed-phase peptide separation kit (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: ... The resuspended peptides were evaluated with the Impact II UHR-TOF MS system (Bruker Daltonics Ltd.) coupled with the nanoLC system (Thermo Fisher Scientific). The protein quantification in individual samples was conducted using Maxquant 1.6.6.0 and the Andromeda search engine to correlate MS/MS spectra to the Uniprot Rattus norvegicus database [21] ...
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bioRxiv - Cell Biology 2022Quote: ... The resulting peptides were analyzed with a nanoAcquity UPLC system (Waters) coupled to an Orbitrap Q Exactive HF mass spectrometer (Thermo Fisher Scientific) employing the LC-MS/MS protocol in a data-independent acquisition mode ...
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bioRxiv - Cancer Biology 2022Quote: ... Purified proteins were on-bead digested and tryptic peptides were analyzed by nanoflow-LC-MS/MS with a high performance nanoflow-HPLC Orbitrap based ms/ms system (Thermo Fisher Scientific). The raw data files were analyzed with MaxQuant software (version 1.5.3.30) ...
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bioRxiv - Immunology 2022Quote: ... followed by in gel tryptic digestion and the resulting peptides in the supernatant were concentrated and analysed by LC-MS/MS on a LTQ-Orbitrap Velos mass spectrometer (Thermo Fisher Scientific) as described (Morikawa et al. ...
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bioRxiv - Immunology 2022Quote: ... and citrullinated Histone 4 using biotinylated peptides (Cit3-His4 bio: SG[Cit]GKGGKGLGKGGAKRHGSGSK-biotin) on streptavidin-coated high-capacity pre-blocked plates (Thermo Fisher Scientific) and hIgG1 at 5 μg/ml in RIA buffer (1% BSA ...
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bioRxiv - Plant Biology 2022Quote: ... the peptides were analyzed by online nano flow liquid chromatography tandem mass spectrometry performed on an EASY-nanoLC 1200 system (Thermo Fisher Scientific) connected to a Q Exactive™ Plus mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: ... Digested peptides were analysed by LC-MS/MS with a Dionex Ultimate 3000 RSLC nanoUPLC (Thermo Fisher Scientific Inc, Waltham, MA, USA) system and a Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific Inc ...
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bioRxiv - Molecular Biology 2022Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive Plus, Thermo Fisher Scientific) using a 50 min gradient ...
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bioRxiv - Microbiology 2022Quote: ... Extracted proteins were subject to in-solution trypsin digestion and the resulting peptides were sequenced on a Q-Exactive Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific) at the Vienna Research Platform for Metabolomics & Proteomics ...