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Citations for Thermo Fisher :
4451 - 4500 of 5129 citations for MRPL54 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: The precipitated pellets were processed as described in the proteomics section and peptides were cleaned up and stored on C18 HyperSEP cartridges at −80°C until use (Thermo Scientific).
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bioRxiv - Immunology 2023Quote: ... used to measure AKT activity and the Z’-LYTE Kinase Assay Kit - Ser/Thr 7 Peptide (Thermo Fisher Scientific, Cat# PV3180) used to measure PDK1 activity ...
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bioRxiv - Biochemistry 2023Quote: ... The concentration of amino acids and unique peptides was measured using an ultrahigh-performance liquid chromatograph tandem triple quadrupole mass spectrometer (Vanquish UHPLC-TSQ Altis MS, ThermoFisher, USA). To confirm the peptide structure ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The pooled and desalted sample was fractionated using high pH reverse-phase chromatography on an XBridge peptide BEH column (130 Å, 3.5 μm, 2.1 × 150 mm, Waters) on an Ultimate 3000 HPLC system (Thermo Scientific/Dionex). Buffers A (10 mM ammonium formate in water ...
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bioRxiv - Cell Biology 2023Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Scientific).
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bioRxiv - Biochemistry 2023Quote: ... Peptides were then separated with an Acclaim PepMap RSLC column (75 µm ξ 25 cm, C18 2 µm 100Å, Thermo Scientific) at 0.25 µL/min with a gradient of 4-31% solvent B (80% I ...
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bioRxiv - Cancer Biology 2023Quote: Peptides were resuspended in 200 mM HEPES (pH 8.5) and a 100µg aliquot of peptides was mixed with tandem mass tags (TMT, ThermoFisher Pierce, Rockford, IL) at a label to protein ratio of 2:1 ...
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bioRxiv - Biochemistry 2023Quote: ... Interaction of His-fused proteins with other purified proteins (e.g. GST-PKAC) or synthetic peptides was studied by pulldown using Ni-NTA affinity resin (HisPur, Thermo scientific # 88221). GST-fused proteins were pulled down on glutathione-Sepharose beads (Amersham Biosciences) ...
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bioRxiv - Biochemistry 2023Quote: ... The soluble fraction was then added to a new tube and the peptides were extracted and desalted using C18 desalting columns (Thermo Scientific).
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bioRxiv - Developmental Biology 2023Quote: ... The resultant peptides were analyzed on an Advance UHPLC system (ABRME1ichrom Bioscience) connected to a Q Exactive mass spectrometer (Thermo Fisher) processing the raw mass spectrum using Xcalibur (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: LC MS/MS data was acquired using a Dionex UltiMate 3000 RSLCnano for peptide separation and analysed with an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Scientific) with an Acclaim PepMap RSLC analytical column (75 µm x 50 cm ...
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bioRxiv - Cancer Biology 2023Quote: ... The peptides were then reconstituted in MS-grade 0.1% FA in water before determining their concentration using a Pierce Quantitative Fluorometric Peptide Assay (Thermo Scientific 23290) following the manufacturer’s protocol.
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bioRxiv - Cancer Biology 2023Quote: Digested peptides were run on a Q-Exactive HF mass spectrometer coupled to an EASY-nLC II 1200 chromatography system (Thermo Scientific). Samples were loaded on a 50 cm fused silica emitter that was packed in-house with ReproSIL-Pur C18-AQ ...
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bioRxiv - Cell Biology 2023Quote: ... and about 1 µg peptide was loaded onto a 2-cm trap column and separated by a 50-cm EASY-Spray column (Thermo Scientific) heated to 55°C ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting peptides were analyzed by online nano liquid chromatography (LC)-MS/MS (UltiMate 3000 RSLCnano and Q-Exactive HF, Thermo Scientific). For this ...
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bioRxiv - Cell Biology 2023Quote: ... TFEB tryptic peptides were dried and reconstituted in LC-MS mobile phase A (2% acetonitrile, Thermo Scientific, cat. no.047138.M6), 98% MilliQ purified water ...
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bioRxiv - Cell Biology 2023Quote: ... The peptides were redissolved in 100mM TEAB buffer and equal amounts of peptides from each sample were labelled with TMT 10-plex Mass Tag labelling kit (#90110, ThermoFisher Scientific). After labelling ...
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bioRxiv - Cell Biology 2023Quote: MS analysis of TMT-labelled peptides was performed on a Q-exactive-HF mass spectrometer coupled with a Dionex Ultimate 3000RS (ThermoFisher Scientific). The detailed LC-MS protocol can be found in the supplemental materials ...
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bioRxiv - Cell Biology 2023Quote: ... and the resulting peptides were fractionated using an Ultimate 3000 nano-LC system in line with an Orbitrap Fusion Tribrid mass spectrometer (Thermo Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Mixed peptides were re-dissolved in 300 µl 0.1% trifluoroacetic acid and fractionated according to the instructions of Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher, Cat# 84868). Samples were eluted by gradient acetonitrile of 10.0% ...
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bioRxiv - Cell Biology 2023Quote: ... Approximately 500ng of each peptide fraction was analyzed by LC-MS on a Velos Orbitrap mass spectrometer (Thermo Scientific, Waltham MA). Online capillary LC ...
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bioRxiv - Cell Biology 2023Quote: ... Slices were subjected to tryptic digest and reductive alkylation using routine procedures and eluted peptides analysed by LC-MS/MS on a UltiMate 3000 RSLCnano System (Thermo Scientific) coupled to a LTQ OrbiTrap Velos Pro mass spectrometer (Thermo Scientific).
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bioRxiv - Molecular Biology 2023Quote: ... The resultant peptides were analyzed on an Advance UHPLC system (AMR/Michrom Bioscience) connected to a Q Exactive mass spectrometer (Thermo Fisher) processing the raw mass spectrum using Xcalibur (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2023Quote: ... the eluates obtained from competitor peptide elutions were resuspended in lysis buffer and incubated with PierceTM anti-HA agarose (Thermo Fisher) for 3 h with end-over-end rotating at 4 °C ...
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bioRxiv - Cancer Biology 2023Quote: ... Trypsinized peptides were then analyzed on an EASY-nLC II system connected to an Orbitrap Elite ETD hybrid mass spectrometer (Thermo Fisher) using Thermo Scientific™ Xcalibur™ Software ...
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bioRxiv - Cell Biology 2023Quote: ... samples were loaded offline onto 3-phase chromatography columns and peptides were eluted using 10 MudPIT steps into an Orbitrap Elite mass spectrometer in positive ion mode (Thermo Scientific) using an Infinity 1260 quaternary pump (Agilent).
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bioRxiv - Microbiology 2024Quote: ... For LC-MS/MS measurements 800 ng of peptides were analyzed on an LTQ-Orbitrap Elite mass spectrometer (Thermo Fisher Scientific) coupled to an EASY-nLCTM 1000 UHPLC system (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2024Quote: LC-MS/SM analysis of digested peptides was performed on an Orbitrap Q Exactive Plus mass spectrometer (Thermo Fisher Scientific, Bremen) coupled to an EASY-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2024Quote: ... at a trypsin:protein ratio of 1:20 and peptides were purified using C18 tips (Pierce C18 Tips, 100-μl bed; Thermo Fisher Scientific). Purified peptides were diluted to 0.1 μg μl−1 and 20 μl (2 μg ...
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bioRxiv - Microbiology 2024Quote: ... 4 µl of peptide mixtures (1µg) were loaded onto an Acclaim PepMap precolumn (75 µm x 2 cm, 3 µm, 100 Å; Thermo Scientific) equilibrated in solvent A and separated at a constant flow rate of 250 nl/min on a PepMap RSLC C18 Easy-Spray column (75 µm x 50 cm ...
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bioRxiv - Cancer Biology 2024Quote: 50 µg of the of the resulting peptides after tryptic digest as described above were subsequently labeled using TMT-10plex reagents (Thermo Scientific) according to manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Peptides were loaded onto a µ-precolumn (Acclaim PepMap 100 C18, cartridge, 300 µm i.d.×5 mm, 5 µm) (Thermo Scientific), and were separated on a 50 cm reversed-phase liquid chromatographic column (0.075 mm ID ...
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bioRxiv - Molecular Biology 2024Quote: ... 1/10 of peptide solution was injected in a nanoRSLC-Q Exactive PLUS (RSLC Ultimate 3000) (Thermo Scientific,Waltham MA, USA). Peptides were loaded onto a µ-precolumn (Acclaim PepMap 100 C18 ...
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bioRxiv - Molecular Biology 2024Quote: ... Separated peptides were eluted directly from the column and sprayed into an Orbitrap Velos Mass Spectrometer (ThermoFisher Scientific, Hemel Hempstead, UK) using an electrospray capillary voltage of 2.7 kV ...
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bioRxiv - Neuroscience 2024Quote: ... Oligomers of Aβ42 were prepared by diluting the peptide solution in phenol red-free F12 medium (Thermo Fisher, Waltham MA, USA) to a final concentration of 100 μM and incubated for 24 h at 4°C followed by immediate use or storage at -80°C ...
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bioRxiv - Bioengineering 2024Quote: ... A one microgram equivalent of peptide was subjected to LCMS/MS analysis on Orbitrap Q-Exactive Plus mass spectrometer (Thermo Scientific) in conjunction with Dionex UltiMate3000 Ultra High-Performance Liquid Chromatography (Thermo Scientific ...
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bioRxiv - Plant Biology 2024Quote: ... 5 µl of this peptide mix was injected onto an Acclaim PepMap RSLC HPLC column (75 µm × 15 cm, Thermo Scientific) connected to the EASY-nLC 1000 system (Thermo Scientific) ...
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bioRxiv - Cell Biology 2024Quote: ... Peptides were loaded onto a trapping microcolumn Acclaim PepMap100 C18 (20 mm x 100 μm ID, 5 μm, 100Å, Thermo Scientific) before separation on a reversed-phase custom packed nanocolumn (75 μm ID × 40 cm ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptides were eluted for 35 minutes onto a 0.075 mm × 500 mm Acclaim™ PepMap™ RSLC resolving column (Thermo Scientific) using a liner gradient ranging from 5-28% of buffer B (80% Acetonitrile/0.1% Formic Acid/19.9% Water ...
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bioRxiv - Cancer Biology 2024Quote: ... Peptide concentration was calculated based on the peak area of 100 ng of Pierce HeLa protein digestion standard (Thermo Fisher Scientific). Afterwards the peptide solution was frozen at −70°C before further processing.
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bioRxiv - Cancer Biology 2024Quote: ... About 5 µg digested peptides were aliquoted for global proteomics analysis by stage tip desalting and dried using Speed-Vac (Thermo Scientific). 1 µg peptides were loaded on Evotip (Evosep Biosystems ...
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bioRxiv - Cell Biology 2024Quote: ... peptide samples were then dried in a speedvac and resuspended in a solution of 3% acetonitrile and 0.1% TFA and desalted using Pierce Peptide Desalting Spin Columns (Thermo Scientific # 89851). Eluants were dried in a speedvac and resusupended in a solution of 3% acetonitrile and 0.1% formic acid to a final concentration of 0.5 µg/µL ...
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bioRxiv - Biochemistry 2024Quote: ... and were subjected to separation on an XBridge Peptide BEH C18 column (Waters) connected to an Accela high-performance liquid chromatography (HPLC) system (Thermo Scientific). A 50-min gradient of buffer B (90% ACN ...
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bioRxiv - Bioengineering 2024Quote: ... to peptides detected using DECODE was performed as follows: 12.5µL per well of 4 mM peptide solutions were coated on 384 well ELISA plates™ (Thermo Fisher Scientific) by shaking at r.t ...
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bioRxiv - Biochemistry 2024Quote: ... cell lysates were spiked with a known concentration of synthetic stable isotope-labeled peptides (Gerber et al, 2003) (AQUA) obtained from Life Technologies: ATPGHTG[C]LSPG[C]PDQPA(R ...
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bioRxiv - Microbiology 2024Quote: ... Eluting peptides were directly analyzed on a Q-Exactive HF mass spectrometer in data-dependent acquisition (DDA) mode (Thermo Fisher Scientific)15 ...
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bioRxiv - Biochemistry 2024Quote: ... Pierce™ Quantitative Colorimetric Peptide Assay (23275) and Pierce™ Rapid Gold BCA Protein Assay Kit (A53227) were ordered from ThermoFisher Scientific (Waltham ...
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bioRxiv - Cell Biology 2024Quote: The resulting peptides were analyzed by LC-MS using Nano LC-MS/MS (Dionex Ultimate 3000 RLSCnano System, Thermo Fisher Scientific) interfaced with Eclipse (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2024Quote: ... Isobaric-labelled peptides from total proteome and phosphoproteomes were then fractionated into eight fractions using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Biochemistry 2024Quote: ... The peptide samples were loaded onto an Acclaim PepMapTM 100 C18 trap column (100 µm x 2 cm, 100 A; Thermo Scientific) and resolved on a PepMap RSLC C18 analytical column (2µm ...