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Citations for Thermo Fisher :
4601 - 4650 of 5129 citations for MRPL54 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2021Quote: ... The FL-NP construct included a C-tag peptide (EPEA) at the C-terminus (27) for affinity chromatography purification using a C-tag affinity resin (Thermo Fisher Scientific), eluting with 20 mM Tris-HCl ...
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bioRxiv - Biochemistry 2020Quote: ... Separation of peptides was achieved by use of an Acclaim Pepmap RSLC analytical column (C18, 100 Å, 75 μm. × 50 cm (Thermo Scientific)) ...
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bioRxiv - Neuroscience 2021Quote: ... The eluted peptides were ionized online by electrospray ionization (ESI) and transferred into an LTQ Orbitrap XL mass spectrometer (Thermo Fisher Scientific) which was operated in the positive mode to measure full scan MS spectra (300–1700 m/z in the Orbitrap analyzer at resolution R = 30,000 ...
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bioRxiv - Neuroscience 2021Quote: ... the peptide extracts were reduced in volume by vacuum centrifugation and a small portion of the extract was used for fluorometric peptide quantification (Thermo scientific Pierce). Samples were analyzed for LC-MS/MS analysis by UC Davis Proteomics core ...
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bioRxiv - Biochemistry 2021Quote: ... The peptides were trapped onto a C18 PepMap 100 pre-column (inner diameter 300 mm × 5 mm, 100 Ǻ; Thermo Fisher Scientific) using solvent A (0.1% formic acid in water ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were separated by nano-flow reversed-phase liquid chromatography coupled to a Q Exactive Hybrid orbitrap mass spectrometer (Thermo Fisher Scientific). The peptides were trapped onto a C18 PepMap 100 pre-column (inner diameter 300 mm × 5 mm ...
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bioRxiv - Neuroscience 2019Quote: Each tryptic peptide mixture obtained from 150 μg of protein extract was isotopically labeled with the corresponding TMT6plex reagent (Thermo Fisher Sci.) based on the standard procedure ...
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bioRxiv - Cell Biology 2021Quote: ... The peptide extracts are reduced in volume by vacuum centrifugation and a small portion of the extract is used for fluorometric peptide quantification (Thermo scientific Pierce). One microgram of sample based on the fluorometric peptide assay was loaded for each LC-MS analysis.
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bioRxiv - Plant Biology 2021Quote: The digested peptide mixtures were injected into a liquid chromatography-tandem MS system using an Ultimate 3000 RSLCnano LC (Thermo Fisher Scientific) in-line connected to a Q Exactive (SAMBA callus versus GIF control group and SAMBA leaf ...
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bioRxiv - Microbiology 2020Quote: ... 2 µl of each sample were subjected to reverse phase liquid chromatography for peptide separation using an RSLCnano Ultimate 3000 system (Thermo Fisher Scientific). Peptides were loaded on an Acclaim® PepMap 100 pre-column (100 µm × 2 cm ...
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bioRxiv - Microbiology 2020Quote: ... The eluted peptides were sprayed into a Q Exactive hybrid quadrupole-Orbitrap mass spectrometer using a Nanospray Flex™ Ion Sources (Thermo Scientific). Survey scans were taken in the Orbi trap (35,000 resolution determined at m/z 200 ...
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bioRxiv - Systems Biology 2021Quote: ... Peptide sample was loaded onto the pre-analytical column (100 µm x 2 cm, nanoViper C18, 5 µm, 100A; Thermo Fisher Scientific) at a flow rate of 5 µl/min ...
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bioRxiv - Synthetic Biology 2021Quote: ... Tryptic peptides were analyzed using an Easy-nLC1000 liquid chromatography system coupled to a high-resolution Q-Exactive mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Plant Biology 2021Quote: ... Samples were sequenced by Edman Degradation using a Perkin Elmer 494 Procise Protein/Peptide Sequencer with an on-line 140C PTH Amino Acid Analyzer (Applied Biosystems, Inc).
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bioRxiv - Microbiology 2020Quote: ... Peptides were loaded onto an Acclaim PepMap100 C18 trap column (300 µm ID × 5 mm, 5 µm, Thermo Fisher Scientific; Cat. # 160454) and then separated on a PepMap RSLC C18 analytical column (75 µm ID×15 cm ...
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bioRxiv - Systems Biology 2021Quote: ... coupled with a customized LC system as previously described.15 The peptide fractions from high pH reversed-phase LC fractionation were analyzed an LTQ Orbitrap Velos mass spectrometer (Thermo Fisher Scientific) coupled with a similar customized LC system ...
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bioRxiv - Microbiology 2021Quote: ... Appropriate correction factors for the individual TMT channels for both lysine side-chain labelling and peptide N-terminal labelling as per the TMT-6plex kits used (Thermo Fisher Scientific) were configured into the database search ...
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bioRxiv - Neuroscience 2020Quote: High pH reversed-phase peptide fractionation kit was used to perform desalting and fractionation as per manufacturer’s protocol (ThermoFisher, Cat. No. 84868). Briefly ...
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bioRxiv - Microbiology 2021Quote: ... Eluted peptides were ionized with a μPAC Flex iON connect (PharmaFluidics, Ghent, Belgium) attached to a Nanospray Flex Ion Source (Thermo Fisher Scientific) equipped with a nanoESI emitter (FOSSILIONTECH ...
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bioRxiv - Plant Biology 2021Quote: ... The OmAGAL2 coding sequence without the expected signal peptide (SP) sequence (ΔSP-OmAGAL, amino acids [AA] 47–412) was cloned into the pENTR/D-TOPO vector (Thermo Fisher Scientific) in accordance with the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: Six μl of resuspended peptides for the glycoform identification and two μl two-fold diluted peptides for the glycoform quantification were run on an Orbitrap Fusion Tribrid (ThermoFisher Scientific, Bremen) mass spectrometer coupled to nanospray UHPLC system Agilent 1290 (Agilent Technologies ...
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bioRxiv - Microbiology 2020Quote: ... The resulted peptides were cleaned up in a HyperSep filter plate with bed volume of 40 μL (Thermo Fisher Scientific, Rockford, IL). Briefly ...
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bioRxiv - Microbiology 2019Quote: ... Peptides were analyzed using a nanoLC-MS/MS system connected to a Q-Exactive hybrid quadrupole-orbitrap mass spectrometer (Thermo Scientific, Germany) equipped with a nano-electrospray ion source ...
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bioRxiv - Cell Biology 2021Quote: 400 ng tryptic peptides per sample were analyzed using an Ultimate 3000 RSLCnano HPLC (Dionex) coupled to a Q Exactive HF Orbitrap (Thermo Fisher Scientific). Samples were pre-concentrated on a C18 trap column (Acclaim PepMap 100 ...
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bioRxiv - Molecular Biology 2022Quote: ... The resultant peptides were analysed on an Advance UHPLC system (AMR/Michrom Bioscience) coupled to a Q Exactive mass spectrometer (Thermo Fisher Scientific), and the raw mass spectrum was processed using Xcalibur (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive Plus, Thermo Fisher Scientific) using a 140-min gradient ...
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bioRxiv - Biochemistry 2022Quote: ... Tryptic peptides were analysed by nano-scale capillary LC-MS/MS with an Ultimate U3000 HPLC (ThermoFisher Scientific Dionex, San Jose, USA) set to a flowrate of 300 nL/min ...
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bioRxiv - Biochemistry 2022Quote: ... Resulting peptides were separated during a 1 h gradient on C18 material using an Ultimate3000 rapid separation liquid chromatography system (Thermo Fisher Scientific) and subsequently injected in an online-coupled QExactive plus mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2022Quote: ... The peptides were separated on a 75 μm × 15 cm RP-HPLC analytical column packed with 2 μM C18 particles (Thermo Fisher Scientific) using an increasing gradient of 0.1% FA in ACN (8-35% over 100 min ...
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bioRxiv - Molecular Biology 2022Quote: ... The eluted peptides were electrosprayed at a 2.0 kV spray and analyzed by an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific, USA) coupled to the LC system ...
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bioRxiv - Genetics 2022Quote: ... The peptide extracts were reduced in volume by vacuum centrifugation and a small portion of the extract was used for fluorometric peptide quantification (Thermo scientific Pierce). One microgram was loaded for each LC-MS analysis.
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bioRxiv - Cell Biology 2022Quote: ... which were subjected to in-gel tryptic digestion and the resulting peptides analysed by nano-liquid chromatography tandem mass spectrometry (LC-MS/MS) using an Orbitrap Fusion Tribrid mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... Peptides were desalted using Sep-Pak® Light C18 Cartridge (Waters, Wilmslow, UK, WAT023501) and dried down using a SpeedVac (Thermo Scientific).
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bioRxiv - Biochemistry 2022Quote: ... Digested peptides were analysed by LC-MS/MS on an Exploris 480 coupled to an Ultimate 3000 nanoLC system (Thermo Fisher Scientific) for figure 2D and on an Exploris 240 (Thermo Fisher Scientific ...
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bioRxiv - Cancer Biology 2022Quote: ... at 300 nL/min was used to elute peptides from the capillary reverse-phase column (0.075 mm × 150 mm, Acclaim Pepmap 100® C18, Thermo Fisher Scientific). Eluted peptides were electro-sprayed online at a voltage of 1.5 kV into the Q-Exactive+ mass spectrometer ...
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bioRxiv - Microbiology 2021Quote: SARS-CoV-2 RBD WT for SPR binding assays (with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) was expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Eluted peptides were analyzed with an Orbitrap Fusion mass spectrometer coupled with an Easy-nLC 1000 (Thermo Fisher Scientific, Waltham, MA, USA). Mass spectrometer was operated in positive polarity mode with capillary temperature of 320 °C ...
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bioRxiv - Cell Biology 2021Quote: One ug of each sample with 50 femtomole of heavy labeled Peptide Retention Time Calibrant (PRTC) mixture (Thermo Fisher Scientific, cat # 88321) was loaded onto a 30 cm fused silica picofrit (New Objective ...
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bioRxiv - Biochemistry 2021Quote: Digested and desalted peptides were analyzed on an Orbitrap Fusion Tribrid mass spectrometer coupled to an EASY-nLC 1200 system (Thermo Fisher Scientific). Tryptic peptides were separated at a flow rate of 300 nl/min using a 190 min gradient from 5% ACN ...
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bioRxiv - Immunology 2021Quote: ... NC_045512.2 with N-terminal signal peptide and C-terminal thrombin cleavage site-TwinStrep-8xHis-tag) were expressed in Expi293F (Thermo Fisher Scientific) cells at 37°C and 8% CO2 ...
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bioRxiv - Bioengineering 2021Quote: ... The amounts of peptides to be tagged were normalized and mixed with 41 μL of TMT reagent (TMT sixplex, Thermo Fisher Sci) freshly dissolved in acetonitrile (20 μg/μL ...
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bioRxiv - Microbiology 2020Quote: ... The peptide extracts are reduced in volume by vacuum centrifugation and a small portion of the extract is used for fluorometric peptide quantification (Thermo scientific Pierce). One microgram of sample based on the fluorometric peptide assay was loaded for each LC-MS analysis.
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bioRxiv - Immunology 2021Quote: ... Equivalent aliquots of the digested peptides were analysed by LC-MS/MS using an Easy-nLC 1200 coupled to Q Exactive HF mass spectrometer (Thermo Fisher Scientific). The peptides were loaded onto a 20 × 0.1 mm i.d ...
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bioRxiv - Cell Biology 2022Quote: ... the peptides were analyzed with a nanoAcquity UPLC system (Waters) coupled to an Orbitrap Q Exactive HF mass spectrometer (Thermo Fisher Scientific) employing the LC-MS/MS protocol in a data-independent acquisition mode ...
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bioRxiv - Microbiology 2022Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific) using a 80 min gradient ...
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bioRxiv - Immunology 2019Quote: ... Acidified eluted peptides were analysed in the data-dependent mode on a Q Exactive HF mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) online coupled to a UItimate 3000 RSLC nano-HPLC (Dionex) ...
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bioRxiv - Pathology 2019Quote: ... of the peptides were analyzed on a Q-Exactive Orbitrap mass spectrometer coupled to an Easy NanoLC-1000 system (Thermo Fisher Scientific). Each sample was analyzed in triplicate ...
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bioRxiv - Genomics 2019Quote: We analyzed fractionated peptides on an Orbitrap Fusion mass spectrometer coupled with the Nanospray Flex ion sources and an UltiMate 3000 UPHLC (Thermo Fisher Scientific). For each run ...
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bioRxiv - Physiology 2019Quote: ... and of those from GnHCl fraction (10µL, corresponding to 5 µg peptides) were loaded onto a spectrometer (Q-Exactive Quadrupole-Orbitrap, Thermo Scientific, Loughborough, UK) on a one-hour gradient with an intersample 30-minute blank loaded.
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bioRxiv - Cell Biology 2020Quote: ... 1.5 μg peptides were resuspended in 1% formic acid diluted in water and loaded on a Q Exactive Orbitrap mass spectrometer (Thermo Fisher Scientific). Settings used were as previously described [22] ...