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Citations for Thermo Fisher :
4751 - 4800 of 5129 citations for MRPL54 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Systems Biology 2023Quote: Acquisition of single-cell derived peptides was conducted with an Orbitrap Astral mass spectrometer operated in positive mode with the FAIMS Pro interface (Thermo Fisher Scientific) using a compensation voltage set to −50 V ...
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bioRxiv - Microbiology 2023Quote: ... was added to cultures to label new peptide synthesis before harvesting (Click-iT™ Plus OPP Alexa Fluor™ 488 Protein Synthesis Assay Kit, Invitrogen)88 ...
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bioRxiv - Cell Biology 2023Quote: ... concentrated using vacuum centrifugation and separated into eight fractions using Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (84868, Thermo Scientific) according to the manufacturer’s instructions for TMT-labelled peptides ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific) using a 140 min gradient ...
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bioRxiv - Microbiology 2023Quote: ... the valve switched to allow peptides to be separated on an Acclaim PepMap C18 nano column (2 μm, 75 μm x 25 cm, Thermo Fisher Scientific) at 35 °C in either 60-minute or 90-minute gradients of 5% to 35% buffer B (98% ACN with 0.1% formic acid ...
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bioRxiv - Microbiology 2023Quote: ... LC-MS/MS analysis of the biotinylated peptides was performed on an EASY-nLC 1200 UHPLC connected to an Orbitrap Fusion mass spectrometer (Thermo Fisher Scientific) as described previously67 ...
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Borrelia PeptideAtlas: A proteome resource of common Borrelia burgdorferi isolates for Lyme researchbioRxiv - Biochemistry 2023Quote: ... B31-Biotin labelled peptides were analyzed using Agilent 1100 nano pump coupled to an LTQ Velos Pro-Orbitrap Elite mass spectrometry (Thermo Scientific, USA). Sample was loaded onto a trap column consisting of a fritted capillary (360 μm o.d. ...
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bioRxiv - Cell Biology 2023Quote: ... Resulting peptides were extracted and analyzed by high-sensitivity LC-MS/MS on an Orbitrap Fusion mass spectrometer (Thermo Scientific, Waltham MA). Proteins were identified by database searching of the fragment spectra against the SwissProt (EBI ...
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bioRxiv - Biochemistry 2023Quote: ... Eluted peptides were subjected to electrospray ionization and then entered an LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo Fisher Scientific). Peptide sequences (and hence protein identity ...
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bioRxiv - Molecular Biology 2023Quote: ... The eluted peptides were dried and resuspended in 0.1% FA before injection LC-MS/MS analysis of the digested peptides was conducted using an Orbitrap Q Exactive Plus mass spectrometer coupled to an EASY-nLC 1200 (Thermo Fisher Scientific). A home-made column (C18 50 cm capillary column picotip silica emitter tip (75 μ diameter ...
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bioRxiv - Molecular Biology 2023Quote: ... Fifty micrograms of peptides/sample were labeled with TMT labels (16-plex) according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA), and pooled ...
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bioRxiv - Plant Biology 2023Quote: ... each sample containing 25 μg of peptide in 50 mM TEAB buffer was combined with its respective 10-plex TMT reagent (Thermo Fisher Scientific) and incubated for 1 h at room temperature ...
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bioRxiv - Microbiology 2023Quote: ... Appropriate correction factors for the individual TMT channels for both lysine side-chain labelling and peptide N-terminal labelling as per the TMT-6plex kits used (Thermo Fisher Scientific) were configured into the database search ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Orbitrap Exploris 480, Thermo Fisher Scientific) using a 180-min gradient ...
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bioRxiv - Biochemistry 2023Quote: ... The peptides were injected into C18 nano-column on an EASY-nLC (Proxeon) coupled with an Orbitrap Velos mass spectrometer (Thermo Fisher Scientific). A ninety-minute gradient was used for separation at a flow rate of 300nL/minutes ...
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bioRxiv - Cell Biology 2023Quote: Samples were prepared for mass spectrometry as previously detailed.19 Freeze-dried peptides were resuspended in 80 µl of distilled water and quantified by the BCA assay (Thermo Fisher Scientific) according to the manufacturer’s instruction ...
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bioRxiv - Bioengineering 2023Quote: ... The concentration of peptide conjugates was determined by the standard Bicinchoninic acid (BCA) assay using Pierce(tm) BCA Protein Assay Kit (Thermo Fisher Scientific). To confirm successful synthesis of peptide conjugates ...
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bioRxiv - Microbiology 2022Quote: ... Generated peptides were analysed by nanoLC-MS/MS on a QExactive□+□mass spectrometer coupled to an EASY-nanoLC-1000 (Thermo-Fisher Scientific, USA). The data were searched against a database containing the Human Swissprot sequences (2017_01) ...
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bioRxiv - Systems Biology 2023Quote: ... 20 to 30 µg of proteins were digested in-gel and the resulting peptides were analysed online using a Q Exactive HF mass spectrometer coupled with an Ultimate 3000 RSLC system (Thermo Fisher Scientific). MS/MS analyses were performed using the Maxquant software (v1.5.5.1 ...
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bioRxiv - Cell Biology 2022Quote: Lyophilized peptide pellets were reconstituted in 100 mM TEAB and concentration estimates were performed spectroscopically using a Nanodrop 1000 (Thermo Fisher Scientific). For each sample ...
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bioRxiv - Cancer Biology 2023Quote: ... 1 mL min-1) and identity of peptides confirmed by high-resolution mass (Q Exactive Orbitrap LC-MS/MS (Thermo Fisher Scientific).
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bioRxiv - Genetics 2023Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific) using 120 min and 90 min acetonitrile gradients for SIN-3 and ARID-1 interactomes respectively ...
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bioRxiv - Microbiology 2023Quote: ... Peptide sequences (and hence protein identity) were determined by comparing the fragmentation pattern acquired by the software program Sequest (Thermo Fisher Scientific) (64 ...
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bioRxiv - Microbiology 2023Quote: ... Eluted peptides were subjected to electrospray ionization and entered into an LTQ Orbitrap Velos Pro ion-trap mass spectrometer (Thermo Fisher Scientific). Peptides were detected ...
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bioRxiv - Molecular Biology 2023Quote: ... the valve switched to allow peptides to be separated on an Acclaim PepMap C18 nano column (2 µm, 75 µm x 25 cm, Thermo Fisher Scientific) at 35 °C in a 90 min gradient of 5% to 40% buffer B (98% ACN with 0.1% formic acid ...
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bioRxiv - Immunology 2023Quote: EAE was induced in 10-12 week old mice by subcutaneous flank injection of 150 μg of myelin oligodendrocyte glycoprotein peptide (residues 35-55; MOG35-55) (Genemed Synthesis, Inc., San Francisco, USA) emulsified in incomplete Freund’s adjuvant (Thermo Fisher Scientific; DF0639606) supplemented with 5 mg/mL heat-inactivated Mycobacterium tuberculosis (Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2023Quote: ... Mixed peptide samples were prepared from 4 μL of each sample and separated using a high pH reversed-phase peptide separation kit (Thermo Fisher Scientific) according to the instructions ...
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bioRxiv - Immunology 2023Quote: ... anti-SIVmac251 Nef mAb (clone 17, epitope peptide corresponding to SIVmac239 Nef 71-80 not including the residue G63: Thermo Scientific/Pierce) manually conjugated with Alexa 488 or Alexa 647 ...
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bioRxiv - Immunology 2023Quote: The desalted tryptic peptides were reconstituted in formic acid/acetonitrile mixture and the peptide amounts were estimated using a NanoDrop-1000 UV spectrophotometer (Thermo Fisher Scientific). The samples were analysed by LC-MS/MS using an Easy-nLC 1200 coupled to Q Exactive HF mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Cancer Biology 2023Quote: EASY nLC 1200 liquid chromatography: Two hundred (200) ng of tryptic peptides were loaded onto an EASY nLC 1200 liquid chromatography (Thermo Fisher Scientific) for proteomic analyses ...
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bioRxiv - Cell Biology 2022Quote: ... Antibodies against ZNF524 were purified and enriched from the serum against this recombinantly expressed His-ZNF524 using the SulfoLink® Immobilization Kit for Peptides (Thermo Scientific) according to manufacturer’s instructions.
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bioRxiv - Molecular Biology 2023Quote: ... The 24 fractions were subsequently dried and the peptides re-dissolved in 5% formic acid and analyzed by nanoLC–MS/MS system Orbitrap Fusion Tribrid Mass Spectrometer (Thermo Fisher Scientific), equipped with an UltiMate 3000 RSLCnano Liquid Chromatography System ...
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bioRxiv - Microbiology 2023Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Fisher Scientific) over a 150 min organic gradient ...
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bioRxiv - Plant Biology 2023Quote: ... The proteins were trypsinized and the resulting peptides were separated by HPLC and analyzed by LC-S/MS on Q-Exactive (Thermo Fisher Scientific). Data analysis was done as described in (Kurmanbayeva et al ...
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bioRxiv - Biochemistry 2023Quote: ... Mixed peptide samples were prepared from 4 μL of each sample and separated using a high pH reversed-phase peptide separation kit (Thermo Fisher Scientific) according to the instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... Eluted phosphopeptide samples from both enrichments were pooled and fractionated into 14 fractions by increasing ACN concentration from 7 % to 50 % using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). Peptide separations of the whole proteome sample were performed using basic reversed-phase chromatography (bRP-LC ...
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bioRxiv - Molecular Biology 2023Quote: Tryptic peptides were separated on Hypersil Gold AQ C18 RP column 100 mm x 1 mm 3 µm 175 Å (Thermo Scientific) using UltiMate 3000 LC system (Dionex) ...
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bioRxiv - Systems Biology 2023Quote: ... Peptides were separated on an analytical column (Easy-Spray™ PepMap™ RSLC C18, 2 μm, 75 μm × 50 cm, Thermo Scientific) at a voltage of about 2.4 kV and at a flow rate of 0.3 μL/min with mobile phases of 0.1% FA in water and in 95% ACN using a linear gradient ...
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bioRxiv - Systems Biology 2023Quote: ... The acetonitrile was evaporated in a SpeedVac at 45°C and peptide concentration was determined by measuring absorbance at 280 nm on a NanoDrop 2000C spectrophotometer (Thermo Fisher Scientific).
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bioRxiv - Cell Biology 2023Quote: ... The enriched phosphopeptides were dried down and fractionated according to manufacturer’s instructions using High pH reversed-phase peptide fractionation kit (Thermo Fisher Scientific, 84868) for a final 6 fractions and subjected to C18 StageTip desalting prior to MS analysis.
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bioRxiv - Molecular Biology 2023Quote: Phosphoribosylated peptides were analyzed on the EASY-nLC 1200 coupled to an Orbitrap Eclipse Tribrid mass spectrometer (ThermoFisher Scientific, San Jose, CA). Peptides were separated on an Aurora UHPLC Column (25 cm × 75 μm ...
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bioRxiv - Neuroscience 2023Quote: ... Peptides were eluted from the S-Traps with 0.2% formic acid in 50% aqueous acetonitrile and quantified using Pierce™ Quantitative Fluorometric Peptide Assay (Thermo Fisher Scientific).
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bioRxiv - Neuroscience 2023Quote: ... Peptides were loaded onto an Acclaim PepMap 100 trap column (75 μm × 2 cm nanoViper, C18, 3 μm 100 Å, ThermoFisher Scientific) at flow rate of 2 μL/min ...
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bioRxiv - Microbiology 2023Quote: ... Each fraction of tryptic peptides was injected and separately analysed on an Ultimate 3000 HPLC system coupled to a QExactive Orbitrap (Thermo Fisher Scientific). The trap (5 mm × 300 µm ...
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bioRxiv - Microbiology 2023Quote: ... 100 μg of protein from each condition was subjected to in-solution digestion before labelling the resultant peptides using the TMT-10plex Isobaric Label Reagent Set (Thermo Fisher Scientific) according to the manufacturer’s protocol ...
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bioRxiv - Microbiology 2023Quote: ... Eluting peptides were ionized by electrospray ionization and analyzed in a Q Exactive HF hybrid quadrupole-Orbitrap mass spectrometer (Thermo Fisher Scientific) with the following parameters ...
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bioRxiv - Molecular Biology 2023Quote: Peptides in the compiled sample were fractionated (8 fractions) using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Cat: 84868; Thermo Fisher Scientific). Prior to liquid chromatography–mass spectrometry (LC-MS ...
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bioRxiv - Cancer Biology 2023Quote: ... The peptide separation for the phosphoproteome analysis was carried out with an Acclaim™ PepMap™ 100 C18 column (Thermo Fisher Scientific) using a 155min non-linear gradient from 3 to 35% of B (0 min ...
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bioRxiv - Cancer Biology 2023Quote: LC-MS/MS of pTyr peptides was carried out on an Agilent 1260 LC coupled to a Q Exactive HF-X mass spectrometer (Thermo Fisher Scientific). Peptides were separated using a 140-minute gradient with 70% acetonitrile in 0.2 mol/L acetic acid at flow rate of 0.2 mL/minute with approximate split flow of 20 nL/minute ...
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bioRxiv - Cancer Biology 2023Quote: ... The resulting peptides were analyzed by online nano liquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive Plus, Thermo Fisher Scientific) using a 120 min gradient ...