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Citations for Thermo Fisher :
4701 - 4750 of 5129 citations for MRPL54 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Plant Biology 2022Quote: LC-MS/MS analyses of all peptide mixtures were done using Ultimate 3000 RSLCnano system connected to Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Fisher Scientific). Prior to LC separation ...
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bioRxiv - Plant Biology 2022Quote: ... truncated SCPSs versions and cysteine-to-serine replacements (all without signal peptide and Stop codon) were cloned into the secretory expression plasmid pPICZalphaA (Thermo Fisher Scientific). Individual cysteine to serine replacements in SCPSs were amplified from synthetic genes (gBlocks ...
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bioRxiv - Neuroscience 2023Quote: ... C18 column-desalted peptides were resuspended with 100 mM HEPES pH 8.5 and the concentrations were measured by micro BCA kit (Fisher Scientific, Cat # PI23235). For each sample ...
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bioRxiv - Microbiology 2023Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific) using a 360-min gradient ...
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bioRxiv - Plant Biology 2023Quote: ... constructs coding for mCherry tagged versions of the peptides were generated in pK7m34GW-rolD∷EGFP destination vector using Multisite Gateway Technology (Thermo Fisher Scientific). The entry clones contained the same promoter fragments in pDONRP4-P1R which were used for complementation experiments ...
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bioRxiv - Immunology 2022Quote: We induced EAE using 8-to 12-week-old female mice that were subcutaneously immunization with a 100 μL emulsion of 100 μg of MOG35–55 peptide (MEVGWYRSPFSRVVHLYRNGK) (Thermo Fisher Scientific) in PBS with CFA containing 200 μg of Mycobacterium tuberculosis H37Ra (Difco) ...
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bioRxiv - Neuroscience 2023Quote: ... Then 2-8 µg peptide was dissolved in 20 µL 100 mM TEAB pH 8.5 and 100 µg TMTpro 16plex labels (Thermo Fisher Scientific A44522) dissolved in 5 µL anhydrous acetonitrile was added to each sample ...
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bioRxiv - Physiology 2022Quote: ... Desalted peptides were analysed using liquid chromatography mass spectrometry (LC-MS/MS) using an EASY-nLC 1200 UHPLC system (Thermo Fisher Scientific) coupled to an Q Exactive HF mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Physiology 2022Quote: ... Sample aliquots containing 25 μg of peptides were dried and labeled with tandem mass isobaric tags (TMT 10-plex, Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2022Quote: LC-MS/MS analyses of purified and desalted peptides were performed on a Dionex UltiMate 3000 n-RSLC system (Thermo Fisher Scientific), connected to an Orbitrap FusionTM TribridTM mass spectrometer (Thermo Fisher Scientific) ...
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bioRxiv - Plant Biology 2022Quote: Nano-scale liquid chromatography (LC) separation of tryptic peptides was performed on a Dionex Ultimate™ 3000 Rapid Separation LC system (Thermo Fisher). The protein digests were loaded onto a 20 μl nanoViper sample loop (Thermo Fisher) ...
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bioRxiv - Microbiology 2022Quote: ... Peptides were separated on an EASY-Spray PepMap RSLC C18 column (2 µm particle size, 75 µm × 50 cm; Thermo Scientific, ES801) heated to 55 °C using a flow rate of 400 nL/min ...
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bioRxiv - Molecular Biology 2022Quote: Label-free peptide samples were subjected to LC-MS/MS analysis on an EASY-nLC 1200 (Thermo Fisher Scientific, San Jose, CA) coupled to a Q Exactive HF Orbitrap mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2022Quote: ... synthesized with an added terminal cysteine residue (New England Peptide) was dissolved in ultrapure water with 5 mM EDTA and mixed with maleimide-activated OVA (Thermo Fisher Scientific) at room temperature for 2 h.
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bioRxiv - Evolutionary Biology 2022Quote: ... 2 μl of each sample were subjected to reverse phase liquid chromatography for peptide separation using an RSLCnano Ultimate 3000 system (Thermo Fisher Scientific). Therefore ...
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bioRxiv - Neuroscience 2024Quote: ... 50 µg of tryptic peptides were used for High pH RP-HPLC using a 25 cm ProSwift™ RP-4H capillary monolithic column (Thermo Scientific) on an Agilent 1200 series HPLC (high-pressure liquid chromatography ...
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bioRxiv - Cell Biology 2023Quote: ... of samples were pooled and separated into 6 fractions by off-line basic reversed-phase (bRP) using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). The fractions were collected in 7.5 ...
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bioRxiv - Plant Biology 2024Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Fisher Scientific) using a 120-min gradient ...
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bioRxiv - Plant Biology 2024Quote: ... 1 to 3 µl of each sample were subjected to reverse phase LC for peptide separation using an RSLCnano Ultimate 3000 system (Thermo Fisher Scientific). Peptides were loaded on an Acclaim PepMap 100 pre-column (100 µm x 2 cm ...
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bioRxiv - Developmental Biology 2024Quote: ... Liquid chromatography-mass spectrometry (LC-MS) analyses of trypsin-digested peptides were performed on a Q Exactive HF-X hybrid Quadrupole-Orbitrap instrument (Thermo Fisher Scientific) coupled with an Easy-nLC 1200 system ...
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bioRxiv - Cell Biology 2024Quote: ... 100 μg of each pooled sample was digested in-solution as described above and fractionated by Pierce™ High pH Reversed-Phase Peptide Fractionation Kit (ThermoFisher Scientific) according to manufacturer recommendations ...
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bioRxiv - Microbiology 2024Quote: ... Peptides were loaded on a trapping column and eluted over a 75μm x 50cm analytical column (Thermo Fisher P/N ES-803) at 300 nL/min using a 1hr reverse phase gradient ...
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bioRxiv - Microbiology 2024Quote: ... Extracted and desalted [45] peptides were analysed on an Easy-nLC 1200 system coupled to a Q Exactive HF mass spectrometer (all Thermo Fisher Scientific) [44] ...
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bioRxiv - Physiology 2024Quote: Peptides for total proteomic analysis were analyzed on a Dionex 3500 nanoHPLC coupled to an Orbitrap Eclipse mass spectrometer (Thermo Fisher Scientific) via electrospray ionisation in positive mode with 1.9 kV at 275 °C and RF set to 30% ...
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bioRxiv - Developmental Biology 2024Quote: ... Aliquots containing 10 μg of peptide from each sample were labeled with tandem mass tag (TMT) 16-plex reagents (TMTpro, Thermo Fisher Scientific), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2024Quote: ... Baker ™) and peptide concentrations were determined from their absorbance at 280 nm using a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific, USA).
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bioRxiv - Molecular Biology 2024Quote: 1.5 µg trypsinized peptides were dissolved in 50 mM TEAB pH 8.5 and labelled using the TMT10plex Isobaric Labeling Reagent Set (Thermo Fisher Scientific, 90111). Each sample was labeled with TMT10plex reagents at a 20:1 label:peptide w/w ratio ...
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bioRxiv - Developmental Biology 2024Quote: ... the samples were used for conjugation of fluorophore to the puromycylated peptides using the Click-iT ® Plus Alexa 647 Fluor ® Picolyl Azide tool kit (Invitrogen). The pellet of gonads was resuspended and incubated with 200 µL Click-iT ® reaction buffer containing 0.5 µL of 500 µM Alexa Fluor ® Picolyl Azide ...
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bioRxiv - Cell Biology 2024Quote: ... and peptides were analysed via a nanospray ion source into a Q Exactive Plus hybrid quadrupole Orbitrap mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Biochemistry 2024Quote: ... Dried samples were redissolved with 300 μL of 0.1% TFA in H2O and further fractionated using high-pH reversed-phase peptide fractionation kits (ThermoFisher, P/N 84868) following the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: Peptides were analyzed by nano-HPLC-tandem mass spectrometry (MS/MS) using an UltiMate 3000 RSLCnano system (Thermo Fisher Scientific, Dreieich, Germany) connected to an Orbitrap Elite mass spectrometer (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... Fractions were spiked with iRT standard peptides and analyzed in technical duplicates via LC-MS/MS on a hybrid quadrupole-orbitrap mass spectrometer (Q Exactive, Thermo Fisher Scientific) hyphenated to a nanoflow chromatography systems (Easy-nLC 1000 UHPLC ...
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bioRxiv - Immunology 2024Quote: Unenriched peptides and enriched phosphopeptides from each fraction were analyzed by LC-MS/MS on an Orbitrap Exploris 480 (ThermoFisher, Bremen, Germany) connected to a Dionex 3700 RSLC Nano using 100 µm PicoFrit column self-packed with 2.2 μm Acclaim RSLC 120 C18 (ThermoFisher) ...
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bioRxiv - Genetics 2024Quote: ... The digested and derivatized histone peptides were diluted in 0.1% TFA and injected to nLC-QqQ MS (Dionex nanoLC and a ThermoFisher Scientific TSQ Quantum). Peptides were first loaded to a trapping column (2 cm × 150 μm ...
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bioRxiv - Immunology 2024Quote: ... A polyclonal antibody against the ROTR-1 protein was raised using the peptide LDRSPPSDDGTQKV (ROTR-1 amino acids 288 to 301) in rabbit (Thermo Fisher Scientific). Anti-ROTR-1 sera was used at a dilution of 1:1000 ...
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bioRxiv - Immunology 2024Quote: ... (i) peptide separation using acetonitrile gradient was run for 120 min,(ii) data was collected on an Orbitrap Fusion (Thermo Fisher Scientific) operated at 120,000 resolution using HCD (higher-energy collisional dissociation ...
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bioRxiv - Cell Biology 2023Quote: LC-MS/MS analyses of all peptide mixtures were done using RSLCnano system connected to Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher Scientific). Prior to LC separation ...
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bioRxiv - Biochemistry 2023Quote: ... cells were plated into 6-well plates at the density of 300000 cells per well and 24h after plating treated with Aβ or p3 peptides diluted in OPTIMEM (Thermo Fisher Scientific) supplemented with 5% FBS (Gibco) ...
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bioRxiv - Cell Biology 2024Quote: Phosphopeptide-enriched and phospho-depleted peptide fractions were analyzed by LC-MS/MS using a Thermo Easy-nLC 1200 (Thermo Fisher Scientific) with a 50 cm (inner diameter 75 µm ...
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bioRxiv - Biochemistry 2024Quote: ... 0.2 µg of peptide sample was analyzed with a RSLCnano UHPLC system coupled to a Q-Exactive mass spectrometer (Thermo Fisher Scientific) using an acetonitrile gradient (3.2% - 60% ...
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bioRxiv - Cancer Biology 2023Quote: ... dried peptides were resuspended in LC buffer and subjected to DDA-MS on a QExactive H-FX mass spectrometer (Thermo Fisher Scientific) equipped with an Easy-nLC 1200 (Thermo Fisher Scientific) ...
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bioRxiv - Biochemistry 2023Quote: ... These peptides were desalted using Oasis HLB cartridges (Waters, Milford, MA) and then dried by Speed Vac (Thermo Fisher Scientific, Bremen, Germany). Then ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptides were separated on an Acclaim PepMap C18 column (id 75 μm, length 50 cm, particle size 2 μm, Thermo Fisher Scientific) using a 10%-45% gradient of solvent B (80% acetonitrile ...
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bioRxiv - Plant Biology 2023Quote: ... 500 ng TMT-labelled peptide sample was measured on an EASY-nLC 1200 system coupled to an Orbitrap Exploris 480 mass spectrometer (Thermo Scientific, www.thermofisher.com). Liquid chromatography was performed using 20 cm fritless silica emitters with 0.75 µm inner diameter (New Objective ...
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bioRxiv - Plant Biology 2023Quote: ... The resulting peptides were analyzed by nanoLC-MS/MS using an Ultimate 3000 RSLCnano coupled to a Q-Exactive HF (Thermo Fisher Scientific) using a 120-min gradient ...
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A network medicine approach to elucidate mechanisms underlying menopause-induced knee osteoarthritisbioRxiv - Pathology 2023Quote: ... analysis of peptide fractions was carried out on an EASY-nLC 1000 coupled to an Orbitrap Eclipse Tribrid mass spectrometer (Thermo Fisher Scientific). Each fraction was loaded onto an Aurora 25cm x 75µm ID ...
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bioRxiv - Immunology 2023Quote: ... Peptides were ionized by electrospray after HPLC separation with a MS-coupled Proxeon EASY-nLC 1200 liquid chromatography system (Thermo Fisher Scientific) using 75 µm inner diameter nanocapillaries with PicoTip emitters (NewObjective # PF360-75-10-CE-5 ...
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bioRxiv - Microbiology 2023Quote: ... The concentration of peptides was determined by measuring UV absorbance at 280 nm or by performing Pierce rapid gold BCA protein assay (Thermo Fisher Scientific) in case of peptides lacking aromatic amino acids ...
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bioRxiv - Molecular Biology 2023Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to MS/MS (Ultimate 3000 and LTQ-Orbitrap Velos Pro, Thermo Fisher Scientific) using a 124 min gradient ...
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bioRxiv - Cancer Biology 2023Quote: ... Peptide sequences (and hence protein identity) were determined by matching protein databases with the acquired fragmentation pattern by Sequest (Thermo Fisher Scientific).