Labshake search
Citations for Thermo Fisher :
4501 - 4550 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2024Quote: ... and the supernatant was replaced with 5 mM glucose-containing RPMI 1640 with Di-4-ANEPPDHQ (Thermo Fisher Scientific) at 2 µg/mL ...
-
bioRxiv - Immunology 2024Quote: ... heated to 70°C for 5 min and ran on a 4-12% Nupage precast gel (Thermo Fisher Scientific). Protein were then detected using InstantBlue™ Coomassie Stain (Abcam ...
-
bioRxiv - Physiology 2024Quote: Platelet intracellular Ca2+ was measured as described previously using 5 µM Fluo-4 fluorescence dye (Invitrogen, Waltham, Massachusetts, USA).20 1 x 105 washed platelets were labeled with Fluo-4 for 30 min at room temperature (RT) ...
-
bioRxiv - Cancer Biology 2024Quote: Passaging of ROBO1+ sorted Rounded/CBC cultures every 4-5 days is best done by incubating cells with 0.25% trypsin-EDTA (Gibco) for 1-2 min at RT/37°C ...
-
bioRxiv - Molecular Biology 2024Quote: 786-O cells (5 × 104) were seeded in 4-well Nunc Lab-Tek II chamber slides (Thermo Fisher Scientific) and cultured for 2 days ...
-
bioRxiv - Molecular Biology 2024Quote: ... 786-O cells (5 × 104) were seeded in 4-well Nunc Lab-Tek II chamber slides (Thermo Fisher Scientific) and cultured for 2 days ...
-
bioRxiv - Pharmacology and Toxicology 2024Quote: ... centrifuged at 500xg for 5 minutes at 4°C using a swinging bucket rotor (Sorvall Legend RT, Thermo Fisher), and then pellets were washed with an additional 1 mL cold lysis buffer and incubated on ice for an additional 5 minutes.
-
bioRxiv - Immunology 2024Quote: ... Cells were stained in FACS buffer (PBS supplemented with 2% FBS and 5 mM EDTA) for 30 min at 4°C using the following antibodies: eFluor450 anti-mouse CD4 (GK1.5; Invitrogen), BV605 anti-mouse CD8 (53-6.7 ...
-
bioRxiv - Biophysics 2024Quote: ... 5 μl of protein lysate was separated by gel electrophoresis on a NuPAGE 4-12% Bis-Tris Gel (ThermoFisher) and transferred by semi-dry transfer in NuPAGE transfer buffer (ThermoFisher ...
-
bioRxiv - Biochemistry 2024Quote: ... RNAs were labeled at room temperature for 4 h in the dark by adding 5’-(iodoacetamido)fluorescein (IAF) (ThermoFisher) dissolved in DMSO to a final concentration of 7.5 mM ...
-
bioRxiv - Cell Biology 2024Quote: ... was boiled for 5 minutes at 95°C and separated on a 4-12% Bis-Tris gel (NP0321BOX, Invitrogen) in MES running buffer (NP0002 ...
-
bioRxiv - Cancer Biology 2024Quote: ... then denatured at 95°C for 5 minutes and loaded on 4-12% NuPAGE™ Bis-Tris gels (Invitrogen). Samples were transferred to a nitrocellulose membrane using a semi-wet transfer ...
-
bioRxiv - Neuroscience 2021Quote: ... Neurons were incubated with 5 μM Fura-2 AM and 0.04% Pluronic F-127 (Thermo Fisher Scientific) for 45-60 min at 37 °C in standard extracellular solution ...
-
bioRxiv - Molecular Biology 2020Quote: ... Acclaim PepMap 100 nanoViper C18 column (Thermo Scientific, 100 µm ID×2 cm, 5 μm, 100 Å) was used as pre-column for sample loading ...
-
bioRxiv - Molecular Biology 2022Quote: ... 100 μl of RNase A/T1 mix (2 μg/μl, 5 U/μl, Thermo Scientific, cat#EN0551) was added to 400 μl of the cleared lysate and incubated for 20 min at room temperature ...
-
bioRxiv - Immunology 2022Quote: ... blocked for 2 hours at room temperature with 1X PBS supplemented with 5% Fetal Calf Serum (Invitrogen) and 0.25% Tween-20 (Invitrogen) ...
-
bioRxiv - Microbiology 2020Quote: ... 5 x 106 S2 cells were seeded in 2 mL Schneider’s Drosophila media (Thermo Fisher, Waltham, Massachusetts) supplemented with 10% heat-inactivated fetal bovine serum in 9.6 cm2 wells of a tissue culture plate and incubated at 26 °C ...
-
bioRxiv - Physiology 2020Quote: ... The membrane was probed with primary antibodies for α-Tubulin (ThermoFisher 322588, clone B-5-1-2), acetylated (Sigma T7451 ...
-
bioRxiv - Immunology 2020Quote: Cells were harvested from the peritoneum by lavage with 5 ml cold PBS + EDTA (2 mM, Gibco). After washing ...
-
bioRxiv - Genetics 2020Quote: ... cells were passaged into differentiation media supplemented with 5-Ethynyl-2’-deoxyuridine (Life Technologies, A10044, 10 µM). On day 6 of differentiation ...
-
bioRxiv - Molecular Biology 2021Quote: 5-ethynyl-2’-deoxyuridine (EdU)-incorporation assay has been performed using a commercially available kit (Invitrogen, C10340). Animals were injected intraperitoneally with 60 µl of EdU (0.5 mg/ml in 50% DMSO and 50% PBS solution ...
-
bioRxiv - Developmental Biology 2021Quote: ... received two intraperitoneal (i.p.) injections of 5-ethynyl-2’-deoxyuridine (EdU) (Thermo Fisher Scientific, Cat. No.: A10044) 1mg each and 4 hours apart ...
-
bioRxiv - Cell Biology 2022Quote: ... then transfected for 5 hours with indicated plasmids in 2 mL Opti-MEM (Invitrogen, Cat. #31985-088) using Lipofectamine 3000 transfection kit (Thermo Fisher ...
-
bioRxiv - Developmental Biology 2024Quote: ... was prepared and added to 426.5 μL 2:1 (vol:vol) 0.1x Tris (Fisher Scientific, cat.# BP152-5)-ethylenediaminetetraacetic acid (EDTA ...
-
bioRxiv - Biochemistry 2024Quote: ... 5 μL of PCR products was run on a 2% E-gel EX Agarose Gel (ThermoFisher #G401002) for 10 minutes according to the settings programmed into the gel system ...
-
bioRxiv - Neuroscience 2023Quote: ... The BV-2 cells were cultured in DMEM supplemented with 5% heat-inactivated fetal bovine serum (Gibco) and 1% penicillin-streptomycin ...
-
bioRxiv - Neuroscience 2023Quote: Pregnant dams were injected intraperitoneally at e12.5 with a solution containing 5-Ethynyl-2’-deoxyuridine (EdU, ThermoFisher) and embryos were collected 2h after ...
-
bioRxiv - Cell Biology 2023Quote: ... and the sample was washed with 1X PBS for 2 x 5 min using a Pipetman (Gibco), then blocked using the blocking solution for 1 h ...
-
bioRxiv - Cell Biology 2023Quote: ... at 150 × 106 cells/dish for 2 h at 37°C/5% CO2 in RPMI-1640 (Gibco) to separate adherent cells ...
-
bioRxiv - Evolutionary Biology 2023Quote: We conducted all experiments using SM/5 media (Loomis and Sussman 1966) (2 g glucose (Fisher Scientific), 2 g BactoPeptone (Oxoid) ...
-
bioRxiv - Neuroscience 2024Quote: ... mixed retinal cultures were loaded with 5 µM Fura-2 AM (acetoxymethyl ester, #F1221; Thermo Fisher Scientific) with 0.01% pluronic F-127 (#P3000MP ...
-
bioRxiv - Developmental Biology 2024Quote: Dams were injected IP with 30 mg/kg body weight of 5-ethynyl-2’-deoxyuridine (EdU; Invitrogen) 12 hours prior to harvesting at E18.5 ...
-
bioRxiv - Synthetic Biology 2024Quote: EVs (2×109 particles in 15 µL) were mixed with a 5 µL sample buffer (NP0007, Invitrogen) and heated at 70 ℃ for 10 min ...
-
bioRxiv - Bioengineering 2024Quote: ... 2 µL mL-1 egg lecithin (5 mM in EtOH) and 1x chemical defined lipid concentrate (Gibco). After incubation for 2 days ...
-
bioRxiv - Cancer Biology 2024Quote: ... permeabilized in 0.3% Triton X-100 for 5 min and blocked in 2% blocking solution (Thermo fisher). Cells were incubated overnight with the primary antibodies washed in PBS and secondary antibodies were applied for 1 hr before counter-staining with DAPI ...
-
bioRxiv - Cell Biology 2024Quote: The EU/EdU co-labelling mix was prepared by combining 100µM BrdU (5-Bromo-2’-deoxyuridine, Invitrogen) and 500µM EU in Nematostella medium with 2% DMSO and 50mM MgCl2 ...
-
bioRxiv - Biochemistry 2024Quote: ... and then loaded onto a trap column (PepMap100 C18, 300 μm × 2 mm, 5 μm; Thermo Scientific) followed by separation on an analytical column (PepMap100 C18 ...
-
bioRxiv - Developmental Biology 2024Quote: ... 2% SDS and 5% glycerol supplemented with AEBSF and Halt protease and phosphatase inhibitors (Thermo Fisher Scientific). Protein concentrations were measured by BCA assay (Thermo Scientific) ...
-
bioRxiv - Developmental Biology 2024Quote: ... We used Click-IT Plus 5-ethynyl-2′-deoxyuridine (EdU) AlexaFluor-555 or 488 (Thermo Fisher C10638) for all the Endoreplication labeling experiments ...
-
bioRxiv - Cell Biology 2020Quote: ... 1000 μg of total protein was incubated overnight at 4 °C with 2 μg α-GFP (Life Technologies/ThermoFisher Scientific ...
-
bioRxiv - Genomics 2021Quote: ... Nuclei were sorted by gating on the DAPI peaks using a BD FACS Aria III (200,000 – 400,000 events) into a small volume of landing buffer (4% BSA in PBS, 2 U/µl Ambion RNase Inhibitor ...
-
bioRxiv - Biochemistry 2020Quote: ... and 4 mM TCEP using a 2 mL 7,000 MWCO Zeba spin desalting column (ThermoFisher Scientific, Waltham MA). The desalted protein was incubated with 40 μL of 10 mM AlexaFluor-647 C2 maleimide (ThermoFisher Scientific ...
-
bioRxiv - Cancer Biology 2020Quote: Transient transfections were performed by adding 2-3μg of the appropriate vector in conjunction with 4-6μL Lipofectamine transfection reagent (Invitrogen). Cell culture media was changed 12 hours post-transfection and replaced with fresh media ...
-
bioRxiv - Biochemistry 2020Quote: ... 2 mL and 4 mL glass vials and ultraclean Silicone/PTFE septa caps (Thermo Fisher, Waltham, MA, USA) were used for biology ...
-
bioRxiv - Neuroscience 2021Quote: ... buffered with 10 mM 4-(2-hydroxyethyl)-1-piperazineethanesulfonic acid (HEPES) (Life Technology, Thermo Fisher Scientific Inc., USA) and coated with 20 μg/mL laminin (Sigma Aldrich ...
-
bioRxiv - Cell Biology 2021Quote: ... onto a Dionex IonPac AS11-HC column (2 mm × 250 mm, 4 µm particle size, Thermo Fisher Scientific) equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm ...
-
bioRxiv - Cell Biology 2021Quote: ... equipped with a Dionex IonPac AG11-HC guard column (2 mm × 50 mm, 4 µm, Thermo Fisher Scientific). The column temperature was held at 30°C ...
-
bioRxiv - Cell Biology 2021Quote: ... Cells were permeabilized in FACS Buffer (DPBS without calcium and magnesium, 4% FBS, and 2 mM EDTA (Gibco)) with 0.5% (w/v ...
-
bioRxiv - Synthetic Biology 2022Quote: ... 3,4-dihydroxystyrene and 2-methoxy-4-vinylphenol concentrations were quantified using a Dionex UltiMate 3000 HPLC (Thermo Scientific) with a Discovery® HS-F5-3 column (15 cm x 2.1 mm ...
-
bioRxiv - Biochemistry 2022Quote: ... and incubated for 2 h at 4°C with 50 µl of IgG magnetic PanMouse Dynabeads (Thermo Fisher) equilibrated with IP buffer ...