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Citations for Thermo Fisher :
4401 - 4450 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2020Quote: ... All samples were analyzed using an Agilent 1100 HPLC system equipped with a MabPac RP column (3 × 50 mm, 4 µm, Thermo Scientific). Elution conditions were as follows ...
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bioRxiv - Molecular Biology 2020Quote: ... adult cardiomyocytes isolated from 3-4 month old mice were washed in PBS containing protease and phosphatase inhibitor cocktail (PIC; Thermo Scientific) and then fixed in 1% formaldehyde at 25°C for 8 min for cross-linking and followed by quenching by 0.125 M glycine at 25°C for 8 min as previously described ...
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bioRxiv - Genomics 2021Quote: ... 3 and 4 and PABPC1 (ON-TARGETplus SMARTpool) or a scramble control (ON-TARGETplus SMARTpool) were performed in Optimem (Life Technologies), using Oligofectamine (Life Technologies) ...
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bioRxiv - Biochemistry 2021Quote: Nucleotide sugars were separated using Inertsil ODS-4 250 × 4.6 mm column with a particle size 3 μm (GL Science) and Vanquish Flex HPLC system (Thermo Fisher Scientific), with 100 mM potassium phosphate buffer pH 6.4 containing 8 mM tetrabutylammonium hydrogen sulphate as buffer A and 70 % solution of buffer A in 30 % acetonitrile as buffer B ...
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bioRxiv - Cancer Biology 2021Quote: ... for 10 min at 70°C and separated on 4-12% Bis/Tris-gels or 3-8% Tris/Acetat-Gels (Thermo Fisher). After separation ...
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bioRxiv - Immunology 2021Quote: ... PBMCs were incubated for 20 min at 37°C in PBS containing 4,4-difluoro-5,7-dimethyl-4-bora-3a,4a-diaza-s-indacene-3-hexadecanoic acid (BODIPY FL C16; 1 μM; Thermo Fisher Scientific). To determine neutral lipid content ...
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bioRxiv - Cell Biology 2023Quote: ... digitonin-solubilized (4 g/g) mitochondria (10–50 μg) were loaded in NativePAGE Novex 3–12% (vol/vol) gels (Life Technologies). After electrophoresis ...
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bioRxiv - Microbiology 2023Quote: ... the indicated amounts of protein sample were boiled at 95°C for 3 min and separated by 4–12% Bis-Tris SDS-PAGE (Thermo Scientific) either in 1x MES buffer (Thermo Scientific ...
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bioRxiv - Neuroscience 2022Quote: ... Electrophoretic run of samples containing 30μg of proteins was performed on precast NuPAGE 3-8% or 4-12% Tris-Acetate Protein Gels (Thermo Fisher Scientific), or on home-made acrylamide gels 8-15% ...
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bioRxiv - Neuroscience 2022Quote: ... Biotinylated glycoproteins were enriched by incubation for 3 hrs at 4°C on a rotating wheel with high affinity streptavidin agarose beads (ThermoFisher Scientific). Extensive washing was performed on a vacuum manifold ...
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bioRxiv - Molecular Biology 2022Quote: ... Then 3 μg of purified recombinant protein was added with 4 μl of RiboLock RNase Inhibitor (Thermo Fisher Scientific, Waltham, MA). The mixture was incubated at room temperature for 1 hour ...
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bioRxiv - Neuroscience 2022Quote: Mammary glands were harvested from lactating GCaMP6f;K5CreERT2 mice diced into 3- to 4-mm3 pieces and loaded with CellTracker Red (1.5 μM, ThermoFisher C34552, ThermoFisher, Waltham, MA) for at least 30 min in DMEM/F12 containing 10% FCS ...
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bioRxiv - Cell Biology 2022Quote: HSC cells atop ECM hydrogels subjected to the different treatments for 3 d were fixed with 4% paraformaldehyde (Thermo Fisher Scientific) at room temperature for 20 min ...
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bioRxiv - Developmental Biology 2022Quote: ... Epithelial sheets of embryos from the same litter were pooled (3-4 embryos per sample) and incubated for 10 min at RT in TrypLE Select Enzyme (Life Technologies/Gibco). Dissociated cells were resuspended in 0.5% BSA ...
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bioRxiv - Neuroscience 2023Quote: ... then incubated for 3 days (room temperature during the day and 4 °C at night) in PAT-NGS containing rabbit anti-GFP (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... BN-PAGE was performed at 4°C on Native PAGE Novex 3-12% Bis-Tris Protein gels (Thermo Fisher Scientific, BN1001) according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: The tadpole was stepwise rehydrated in 1x PBS with 0.1% Triton X-100 (PBST) and blocking was performed for 3 days at 4°C in 10% CAS-Block / 90% PBST (008120, Life Technologies). Staining was performed using Atp1a1 antibody (1:500 ...
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bioRxiv - Immunology 2023Quote: ... Eight days after subcutaneous immunisation of C57BL/6 mice with 50 μg of 4-Hydroxy-3-nitrophenyl acetyl hapten conjugated chicken gamma globulin (NP-CGG) in Imject Alum Adjuvant (ThermoFisher Scientific), 4.3 µg MAR hypoxia detecting probe (Goryo Chemical ...
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bioRxiv - Molecular Biology 2023Quote: ... and blotted on two sides for 3 sec at 4 °C and 100% humidity before vitrified in liquid ethane using a Vitrobot Mark IV (Thermo Fisher). The grids were subsequently transferred to a Titan Krios (G3i ...
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bioRxiv - Immunology 2023Quote: ... cDNA was diluted 1:3 or 1:4 before mixing with Power SYBR Green PCR Master Mix (CAS: 4368702, Applied Biosystems) and primer pairs (Table S4B) ...
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bioRxiv - Cell Biology 2022Quote: ... 3.5 - 4 ml aliquots of the resulting 10,000xg supernatants were added up with 3-2.5 ml 1X PBS (Gibco, Carlsbad, CA, USA) to reach a final volume of 6.5 ml and were transferred on top of a 60% - 10% iodixanol gradient (Progen Biotechnik GmbH ...
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bioRxiv - Microbiology 2022Quote: ... After 3 hours a fraction of the bacteria was fixed by the addition of 4% methanol-free paraformaldehyde (PFA, Thermo Scientific) for 10 min at room temperature and the reaction was quenched by the addition of 0.3 M glycine ...
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bioRxiv - Neuroscience 2023Quote: ... cells were fixed with 4% PFA for 20 min at RT and then washed (3×) in DPBS with calcium and magnesium (DPBS +/+, Gibco Invitrogen). For structural proteins ...
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bioRxiv - Cancer Biology 2024Quote: ... these plasmids were transfected into HEK293T cells alongside psPAX2 and pMD2.G at a mass ratio of 1 pMD2.G : 3 psPAX2 : 4 pLKO.1 using Lipofectamine 2000 (Invitrogen 11668019), and media containing the lentivirus was concentrated with Lenti-X Concentrator (Takara Bio 631232 ...
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bioRxiv - Molecular Biology 2024Quote: ... Recombinant bacmids were transfected into Sf9 cells to generate recombinant virus and amplified for large scale protein purification by growth to 3-4×106 cells/mL in Sf-900 II SFM media (ThermoFisher #10902088) in a humidified 28°C incubator shaking at 125 rpm ...
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bioRxiv - Microbiology 2024Quote: ... Samples were incubated at 95°C for 3 min and 10 µL were separated by 4–12% Bis-Tris SDS-PAGE (Thermo Scientific) in 1x MOPS buffer (Thermo Scientific) ...
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bioRxiv - Immunology 2024Quote: ... 1 x 109 SupT1 CD4+ T cells were infected with 4 055 ng of VSV-G-HIVNL4-3 XCS HIV-Gagp24 for 3h in IMDM plus 10mM HEPES (Gibco/ThermoFisher Scientific) supplemented with 2 µg/ml of DEAE-dextran (Sigma) ...
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bioRxiv - Biochemistry 2024Quote: The sample was blotted for 3 s with blot force 20 at 100% relative humidity and 4 °C using a Vitrobot Mark IV (Thermo Scientific). Grids were plunge frozen in liquid ethane cooled by liquid nitrogen ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were assayed using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™ ...
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bioRxiv - Bioengineering 2021Quote: ... Medium was changed every 2-3 days and cell passages were carried out using a TrypLE Express solution (Gibco). Human TERT-immortalized gingival fibroblasts (hTERT-HGF ...
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bioRxiv - Neuroscience 2020Quote: ... pooled and purified cDNA libraries with mean sizes of 550-600 bp and concentrations of 2-3 ng/µl (measured with Qubit 2.0, Invitrogen) were sequenced on a HiSeq 2500 Illumina system with single-end 50 bp read lengths ...
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bioRxiv - Cell Biology 2021Quote: ... 2 and 3 (100 pmol for each) or control siRNA were transfected into HeLa cells using Lipofectamine 2000 (Invitrogen). 48 h after transfection ...
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bioRxiv - Microbiology 2021Quote: ... After incubation for 2 h at 37°C the medium was aspirated and 3 mL of fresh DMEM (Gibco) supplemented with 5% New Born Calf Serum (NBCS ...
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bioRxiv - Microbiology 2021Quote: ... The other half of the wells of the Caco-2 cells was subsequently incubated anaerobically for 3 h with 0.3% gentamicin (50 μg/ml, Gibco) to eliminate all extracellular L ...
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bioRxiv - Microbiology 2021Quote: ... The other half of the wells of the Caco-2 cells was subsequently incubated anaerobically for 3 h with 0.3% gentamicin (50 µg/ml, Gibco) to eliminate all extracellular L ...
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bioRxiv - Cell Biology 2021Quote: ... under 5% CO2 at 37°C in humidifying conditions and passaged every 2-3 days using 0.25% Trypsin-EDTA (25200056, Gibco). Cells were routinely tested for mycoplasma ...
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bioRxiv - Cancer Biology 2021Quote: Relative cell proliferation rates were determined using an MTT (3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide) assay kit (Vybrant™ MTT Cell Proliferation Assay Kit, Invitrogen™ ...
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bioRxiv - Microbiology 2022Quote: ... A total of 3 µg of RNA was DNAse treated using 2 units of Turbo DNase I enzyme (Invitrogen). A total of 1 µg of DNAse-treated RNA was reverse transcribed using Superscript III reverse transcriptase (Invitrogen/Thermo Fisher) ...
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bioRxiv - Molecular Biology 2022Quote: ... Cell viability was determined by the MTT [3-(4,5-di-methylthiazol-2-yl)-2,5-diphenyltetrazolium bromide] (Invitrogen, Waltham, MA) reduction assay ...
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bioRxiv - Molecular Biology 2022Quote: ... Secondary antibodies were applied for 2 hours in PBS/3% milk powder containing 1 μg/ml Hoechst-33342 (Invitrogen) or DAPI (Roche ...
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bioRxiv - Cancer Biology 2021Quote: ... conditioned media was replaced by a 1.2 mM 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Thermo Fisher Scientific) solution ...
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bioRxiv - Immunology 2020Quote: ... 1×106 cells per condition were plated in 96 U-shape well plates (CELLSTAR, Kremsmünster) and rested for 2-3 h in RPMI (Gibco), supplemented with 10% FBS ...
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bioRxiv - Synthetic Biology 2020Quote: ... and cell viability was then determined using the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) cell viability assay (Invitrogen, USA) according to the manufacturer’s protocol 28 ...
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bioRxiv - Synthetic Biology 2021Quote: ... The reaction solution was vortexed for 2-3 seconds to avoid bubbles and added to 384-well plate (Nunc™ MicroWell™ 384-Well Optical-Bottom Plates ...
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bioRxiv - Cell Biology 2020Quote: ... 2×106 NP-TTD were transfected with 3 µg plasmid via electroporation using Neon™ Transfection System (MPK1096, Invitrogen) with following parameters ...
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bioRxiv - Neuroscience 2021Quote: ... Activated/Cleaved Caspase-3 (1:1000; Cell Signalling Biology, CAT#: 9661S and Thermo Fisher Scientific, CAT#: 66470-2-IG), Nestin (1:1000 ...
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bioRxiv - Molecular Biology 2023Quote: ... coupled to an Acclaim PepMap 100 column (2 cm x 75 μm, 3 μm particle size, Thermo Fisher Scientific) coupled in line with an EASY-Spray PepMap RSLC C18 reversed-phase column (50 cm x 75 μm ...
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bioRxiv - Microbiology 2023Quote: ... tilt series were acquired at binning 2 on a Falcon 3 camera under the controls of Tomography software (ThermoFisher). For cryo-ET of the enrichment culture ...
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bioRxiv - Neuroscience 2022Quote: ... We reverse-transcribed ∼2-3 µg DNase treated RNA into cDNA using Superscript III Reverse Transcriptase (Invitrogen no. 18080093). For each reaction ...
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bioRxiv - Genomics 2023Quote: ... and 100 μg/ml cycloheximide using Beckman Coulter UC tubes 9/16 x 3-1/2 (Fisher Scientific, NC9194790), and equilibrating overnight at 4 °C ...