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Citations for Thermo Fisher :
4351 - 4400 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2023Quote: ... Takara)-coated plates at an MOI of 5 and the transduced population was enriched by puromycin (3 µg/mL; Gibco, China) selection for 5 days ...
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bioRxiv - Biochemistry 2023Quote: Labeled RNA oligonucleotide ‘20.25’ was annealed to non-labeled complementary RNA oligonucleotide ‘19.1’ (5’-CGU ACG CGG AAU ACU UCG A-3’, Dharmacon, Thermo Scientific) in 30 mM HEPES KOH pH 7,5 ...
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bioRxiv - Biophysics 2023Quote: ... the tissue was incubated for 5 min with 1 µM TO-PRO-3 iodide (Invitrogen, Life Technologies Corporation, Oregon, USA) followed by washing with 1x DPBS (nuclei data not shown) ...
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bioRxiv - Developmental Biology 2023Quote: ... R 5’-atggatccTCATAGAGCTGAAGCCACCAG-3’) were generated by PCR amplification from 24 hpf whole embryo cDNA and cloned to pCRII-TOPO (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... An internal standard was prepared from the isotope-labeled standards in 3% acetonitrile with 0.1% formic acid with 5 fmol/µL of Peptide Retention Time Calibration (PRTC) mixture (ThermoFisher Scientific) and 10 µg/mL E ...
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Mechanisms of transcriptional regulation in Anopheles gambiae revealed by allele specific expressionbioRxiv - Genetics 2023Quote: RNA was extracted from pools of 10 female F1 progeny from each of the six crosses 3-5 days after eclosion using RNAqueous4PCR total RNA isolation kit (Invitrogen). RNA quality and quantity was checked to be adequate for library preparation using the Agilent Bioanalyser profile and Qubit® 2.0 Fluorometer ...
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bioRxiv - Cell Biology 2023Quote: ... the iMACs were washed 3 x 5 min with PBS and incubated for 1h with 1:1000 goat anti-mouse IgG-AF488 (A11001, Invitrogen). Afterwards the cells were again washed 3 x 5 min with PBS ...
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bioRxiv - Developmental Biology 2023Quote: ... was synthesized on templates that contain the T7 promoter sequence (5’-TAATACGACTCACTATAGGGTACT-3’) at each end using a MEGAscript T7 kit (Ambion); templates were amplified from 0–4 h embryonic cDNA using specific primers (5’-AGCAGATGACGACGACAACA-3 ...
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bioRxiv - Neuroscience 2024Quote: ... and Biotinylated Dextran Amine (BDA, cases AV, AT: equal parts 10% 10 kDA and 10% 3 kDA, 5 µl; Invitrogen). Dextran amines with molecular weight 10 kDA (BDA in AT and AV ...
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bioRxiv - Neuroscience 2024Quote: ... Thereafter, sections were rinsed as before (3 x 5 min, cold TBS) then incubated with AlexaFluor conjugated secondary antibodies (Thermofisher) in 5% normal goat serum in TBS for 2 h at 4°C with agitation ...
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bioRxiv - Pathology 2024Quote: ... right arm target 5’-ACA TCG CTA ACC TGC TG CT-3’) were synthesized using the mMESSAGE mMACHINE kit (Ambion) and injected into one-cell stage embryos at 100 pg per embryo ...
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bioRxiv - Neuroscience 2024Quote: ... Tissues were dissociated using 0.05% Trypsin for 5 min at room temperature and subjected to 3 washes with Hanks’ Balanced Salt Solution (HBSS, Gibco). Cell supernatants were triturated in DMEM media with 20% fetal bovine serum using a 1000 μL pipette and applied to a 100 μm cell strainer (MidSci ...
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bioRxiv - Microbiology 2024Quote: ... was cultured at a density of 3 × 10^5 cells/ml in RPMI-1640 medium (Roswell Park Memorial Institute Medium, Gibco), supplemented with 10% (v/v ...
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bioRxiv - Genomics 2024Quote: The cell line containing the Cre-Blasticidin and the CMV-GFP cassettes were cultured for 3-5 days in IMDM media (Gibco, with 10% FBS and 1% Penicilin/Streptomycin ...
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bioRxiv - Immunology 2024Quote: ... Excess secondary antibodies were washed off with PBS (3 washes, 5 minutes each) followed by addition of 32.4 μM of Hoechst nuclear staining reagent (ThermoFisher #H3570) to label nuclei ...
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bioRxiv - Cancer Biology 2024Quote: ... Human lung fibroblast MRC5 and its SV40-transformed derivatives were cultured in a 5% CO2 and 3% O2-regulated incubator in MEM medium without Phenol Red (Gibco), completed with 10% FBS ...
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bioRxiv - Plant Biology 2024Quote: Ribosome footprints were prepared from the youngest fully expanded leaf from 5 week old tobacco plants or from 3-week old Arabidopsis seedlings using Ambion RNase I (Invitrogen) as described previously (Chotewutmontri et al. ...
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bioRxiv - Neuroscience 2024Quote: ... cells were washed 3 times with 1X PBS for 5 minutes each and incubated with secondary antibodies Alexa Fluor 633 (A21094, Thermofisher), Alexa Fluor 555 (A21422 ...
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 80–100 pairs of ovaries from 3- to 5-day-old flies (for the analysis of piRNA production in somatic follicle cells) using TRIzol Reagent (Ambion). After 2S rRNA depletion ...
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bioRxiv - Molecular Biology 2024Quote: ... The siRNA sequences targeting human TFEB (5□-GAA AGG AGA CGA AGG UUC AAC AUC A-3□) were purchased from Invitrogen. The siRNA sequences targeting human NPC1 (5□- CAA UUG UGA UAG CAA UAU UTT-3□ ...
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bioRxiv - Bioengineering 2024Quote: ... KTB21 human mammary basal epithelial cell line was cultured in epithelial cell growth medium (DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific] ...
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bioRxiv - Genomics 2024Quote: ... Media was changed daily and 3×10^5 cells were passaged every other day using TryplE (Life technologies, Cat.N: 12604013) with a 30-minute selective sedimentation step where the detached cells were kept in an uncoated 10cm dish at 37C to separate ES from MEFs ...
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bioRxiv - Biochemistry 2024Quote: ... the cells were washed 3 times with imaging buffer and incubated in imaging buffer containing 5 μM EGTA-AM (#E1219, Invitrogen). After 45 min of incubation ...
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bioRxiv - Biochemistry 2024Quote: siRNA oligonucleotides targeting DEK (5’-GAAGGCTAAGCGAACCAA A-3’) at a final concentration of 50 nM were transfected into cells using Lipofectamine 3000 (Invitrogen) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: Antibodies were obtained in >50 ug quantities and labeled with bifunctional 5’ Acrydite - bit sequence - 3’ DBCO oligos (IDT) by enzymatic modification and click chemistry (SiteClick Antibody Azido Modification Kit, ThermoFisher) according to the manufacturer’s instructions ...
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bioRxiv - Genomics 2024Quote: ... LNCaP cells were seeded at 80% confluence and grown for 3 days in phenol red-free RPMI Medium with 5% charcoal-stripped Fetal Bovine Serum (FBS; #cat 12676029, Gibco) and then stimulated with 10 nM 5-α-dihydrotestosterone (DHT ...
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bioRxiv - Bioengineering 2024Quote: ... Primary cells were cultured in basal medium ((DMEM [low glucose]: Ham’s F12 [1:3] medium supplemented with 5% FBS [Thermo Fisher Scientific] ...
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bioRxiv - Biochemistry 2024Quote: ... 3 µl sample was applied to glow-discharged grids and incubated for 5 s followed by blotting for 3 sec using a Vitrobot Mark IV (FEI ThermoFisher) operated at 4 °C and 100% humidity ...
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bioRxiv - Cancer Biology 2024Quote: ... The tissue was washed 3 x 5 min with PBS and thereafter mounted with Prolong Diamond Antiface Mountant (ThermoFisher, P36961). The tissue samples were placed in the dark at RT for at least 1 day before imaging.
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bioRxiv - Cell Biology 2024Quote: ... Coverslips were washed 3 times for 5 min with 1x PBS before mounting onto glass slides using ProLong Diamond Antifade mountant (Invitrogen) and left to dry overnight at 4°C before imaging.
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bioRxiv - Genetics 2024Quote: The resulting cDNA was amplified with primers SD6-PSPL3_RT-FW (5’-TCACCTGGACAACCTCAAAG-3’) and RTpSAD-RV (CSIC Patent P201231427) using Platinum Taq DNA polymerase (Life Technologies) and the following cycling conditions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were analyzed with the MACSQuant cell analyzer (3 lasers, 10 parameters, Miltenyi Biotech) or the AttuneNxT acoustic focusing cytometer (4 lasers, 14 parameters, Thermo-Fisher Scientific). Data were processed using FlowJo software (ver.9.9 or 10.7 ...
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bioRxiv - Immunology 2021Quote: ... was added to a concentration of 1X and 12.5-30 μg of total protein from each sample was resolved on NuPAGETM 4-12% BisTris (Phospho-PMK-1 and Total-PMK-1) or NuPAGETM 3-8% TrisAcetate (TIR-1::3xFLAG) gels (ThermoFisher Scientific), transferred to nitrocellulose membranes using a Trans-Blot Turbo Transfer System (Bio-Rad Laboratories ...
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bioRxiv - Neuroscience 2021Quote: ... Membranes were then washed 3 times for 4 min with TBST and treated with Pierce ECL Western Blotting Substrate (Thermo Scientific) according to manufacturer protocol ...
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bioRxiv - Microbiology 2020Quote: ... The purified linearized vector and digested foxA gene were ligated (1:3 molar ratio) overnight at 4°C using T4 DNA ligase (Thermo Scientific). The construct was transformed into competent E ...
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bioRxiv - Genomics 2020Quote: ... where 2–3 mm of the terminal ends of 3–4 filaments from the left side each fish were collected and placed in RNAlater (Thermo Fisher Scientific ...
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bioRxiv - Neuroscience 2020Quote: ... Slices were then rinsed 4 x 15 min in PBS using the nuclear counterstain TO-PRO-3 Iodide (1:2000, Thermo Fisher). For preservation of immunofluorescence ...
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bioRxiv - Bioengineering 2021Quote: ... an Agilent LC-MS system consisting of a 1100 HPLC and a 1946D single quadrupole ESI mass spectrometer equipped with a MabPac RP column (3 × 50 mm, 4 µm, Thermo Scientific) or 2 ...
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bioRxiv - Bioengineering 2020Quote: ... We performed ERBB2 SNAPD on a 9:1 mixture of MOLT-4 and SK-BR-3 cells stained with CellTrace™ Calcein Red-Orange AM (Invitrogen). We then reinjected these droplets onto a dielectric sorting device (Fig ...
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bioRxiv - Neuroscience 2020Quote: ... for 1 h at RT and then incubated for 3 days at 4°C with the monoclonal mouse anti-NeuN antibody conjugated with Alexa-488 (clone A60, Life Technologies) at a concentration of 0.5 µg mL-1 into blocking buffer ...
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bioRxiv - Cell Biology 2020Quote: ... were loaded on to NuPAGE 4-12% Bis-Tris or 3-8% Tris-Acetate 1.0 mm Mini Protein Gels (Invitrogen, NP0321, EA0375PK2) buffer alongside PageRuler Protein Ladder (5 µL/lane ...
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bioRxiv - Neuroscience 2022Quote: ... CAT-1 (encoded by SLC7A1) and CAT-3 (encoded by SLC7A3) had 4 substrates including L-Lysine hydrochloride (Fisher Scientific #BP386), L-Histidine (Sigma-Aldrich #H6034) ...
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bioRxiv - Molecular Biology 2022Quote: ... 4 µg of an HA-tag antibody (MBL, M132-3) was mixed with 20 µL of Dynabeads Protein G (Invitrogen, #10004D) and subsequently added to extracts ...
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bioRxiv - Neuroscience 2022Quote: ... Samples were subjected through high pH reverse phase fractionation into 3 or 4 fractions and all fractions were run independently on an Orbitrap Fusion (Thermo Scientific) mass spectrometer coupled to an Acquity M-Class nanoLC (Waters Corporation) ...
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bioRxiv - Immunology 2022Quote: ... Proteins and DNAs were incubated for 30 min at 4 °C and analyzed on 3-12% gradient Bis-Tris native gels (Life Technologies) at 4 °C ...
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bioRxiv - Neuroscience 2022Quote: Cultured neurons at 19-21 DIV were incubated with 3 μM of the calcium dye Fluo-4 AM (Thermo Scientific, Sweden) in DMSO(Sigma-Aldrich ...
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bioRxiv - Microbiology 2020Quote: ... followed by incubation for 3 hr at 4°C with 250 μg of GST-fusion protein coupled to glutathione-agarose (Thermo Scientific). Beads were then washed four times (pull down buffer containing 0.1% Triton X-100 ...
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bioRxiv - Immunology 2020Quote: ... RAG was reconstituted in RAG1-deficient v-Abl cells via retroviral infection of cells with the pMSCV-RAG1-IRES-Bsr and pMSCV-FLAG-RAG2-GFP vectors followed by 3-4 days of Blasticidin (ThermoFisher, #R21001) selection to enrich for cells with virus integration.
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bioRxiv - Cell Biology 2021Quote: ... were isolated from 3-4 days old euthanized rats and were plated in α-MEM medium supplemented with 10% fetal bovine serum (Gibco) and 1% Penicillin-Streptomycin solution (Hyclone ...
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bioRxiv - Bioengineering 2020Quote: ... The reaction was monitored using an Agilent G1946D LC/ESI-MS system equipped with a MabPac RP column (3 × 50 mm, 4 µm, Thermo Scientific). Elution conditions were as follows ...