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Citations for Thermo Fisher :
4451 - 4500 of 10000+ citations for 2 3 5 6 Tetrahydroxy 4 phosphonooxycyclohexyl dihydrogen phosphate since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... equipped with a trapping column (3 µm C18 particle, 2 cm length, 75 µm ID, Acclaim PepMap, Thermo Scientific) and a 50 cm long separation column (2 µm C18 particle ...
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bioRxiv - Genomics 2023Quote: ... and 100 μg/ml cycloheximide using Beckman Coulter UC tubes 9/16 x 3-1/2 (Fisher Scientific, NC9194790), and equilibrating overnight at 4 °C ...
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bioRxiv - Molecular Biology 2023Quote: ... coupled to an Acclaim PepMap 100 column (2 cm x 75 μm, 3 μm particle size, Thermo Fisher Scientific) coupled in line with an EASY-Spray PepMap RSLC C18 reversed-phase column (50 cm x 75 μm ...
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bioRxiv - Microbiology 2023Quote: ... tilt series were acquired at binning 2 on a Falcon 3 camera under the controls of Tomography software (ThermoFisher). For cryo-ET of the enrichment culture ...
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bioRxiv - Cancer Biology 2023Quote: Mouse primary myoblasts were isolated from limb muscles of pups (2-3 days old) and cultured in Ham’s F-10 nutrient mixture (Invitrogen) with 20% FBS (Invitrogen ...
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bioRxiv - Plant Biology 2023Quote: ... cDNAs were synthesized from 2 to 3 μg of total RNA using SuperScript IV and Oligo dT12-18 (Thermofisher) following manufacturer’s instructions ...
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bioRxiv - Neuroscience 2023Quote: ... followed by seeding of 2-3×106 cells onto T25 flasks (Thermo-Fisher Scientific; Cat. No. 12-556-009) coated with Matrigel ...
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bioRxiv - Cell Biology 2023Quote: Mouse primary myoblasts were isolated from the limb muscles of 2-3 day-old pups and cultured in Ham’s F-10 nutrient mixture (Invitrogen) as described before.38 The culture medium was supplemented with 20% fetal bovine serum (FBS ...
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bioRxiv - Biochemistry 2023Quote: ... The peptides were first concentrated on a precolumn (PepMap100 C18 3 μm; 75 μm × 2 cm; Thermo Fisher Scientific) and then separated on an EASY-Spray column (ES903 ...
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bioRxiv - Immunology 2024Quote: ... The transduced cells were further cultured for 3 days under antibiotic selection pressure (2 μg/mL puromycin (Thermo Fisher)) until the non-transduced control cells were dead ...
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bioRxiv - Neuroscience 2024Quote: ... the membrane was incubated in 10 mL of 3% BSA/TBST (w/v) with 2 µL streptavidin-HRP (1:5,000; S911, Invitrogen) for 1 hour at room temperature ...
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bioRxiv - Molecular Biology 2024Quote: ... and transfected with various plasmids using 3 μl Lipofectamine 3000 reagent and 2 μl P3000 reagent (both from Invitrogen). The total amount (1 μg ...
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bioRxiv - Cell Biology 2024Quote: ... The medium was changed every 2-3 days and cells were passaged at <95% confluency with 0.05% Trypsin-EDTA (Gibco).
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bioRxiv - Cell Biology 2024Quote: ... Cells that stably expressed proteins tagged with Halo7 or mGFP were established by using G418 (Nacalai Tesque, 0.2 mg/mL for PC-3 and 2 mg/mL for 4175-LuT) or blasticidin (Invitrogen, 2 mg/mL for PC-3 and 1 mg/mL for 4175-LuT) ...
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bioRxiv - Cancer Biology 2024Quote: ... CIOV1, 2, 3 cell lines (Brenton Lab, University of Cambridge, UK) were cultured in DMEM/F-12 medium (Gibco) with 10% FBS and 1% penicillin/streptomycin ...
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bioRxiv - Cancer Biology 2024Quote: ... as the function of cell health was detected via 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT, Invitrogen, M6494) assay (Ricci et al. ...
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bioRxiv - Cell Biology 2020Quote: ... Supernatant (1 ml at ~4-5 mg/ml) was incubated with 50 μl of superparamagnetic Dynabeads Streptavidin C1 (Invitrogen) and rotated overnight at 4 °C ...
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bioRxiv - Cell Biology 2020Quote: ... Samples were washed for 4 x 5 min in PBS and mounted in Prolong Gold with DAPI stain (Invitrogen). Imaging was performed on an Axio Observer microscope (Zeiss ...
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bioRxiv - Genomics 2020Quote: ... 5 mM MgCl2, 1 mM EGTA, 0.5 mM DTT, 0.05% Tween-20, 4 U/mL SUPERase-In [Thermo Fisher] ...
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bioRxiv - Microbiology 2021Quote: ... Harvested cells were normalized to the lowest OD-600 of the collected cells and pelleted by centrifugation at 20000XG for 2 mins and resuspended in 75 µL lysis buffer (10 mM Tris-HCl, pH 7.0, 4% SDS, 5 U DNaseI (ThermoFisher), 0.2 tablets of PhosStop (Roche)) ...
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bioRxiv - Neuroscience 2020Quote: ... at 95 °C for 5-10 min and loaded on a gradient 4-12% NuPAGE Bis-Tris gels (Invitrogen). After electrophoresis at 200 V ...
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bioRxiv - Microbiology 2020Quote: ... Cell envelopes were stained by adding 5 µL of 1 mg/mL FM™ 4-64FX (Thermo Fisher Scientific) and non-viable cells stained with 1 µL of the LIVE/DEAD™ cell dye (Thermo Fisher Scientific) ...
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bioRxiv - Cell Biology 2022Quote: ... Remnant villi were removed by shaking tissue pieces at 4°C in an initial 5 minute incubation PBS with 10 mM RNase-free EDTA pH 8.0 (Invitrogen). Crypts were collected and filtered through 70 µm cell strainers in subsequent fractions ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated with primary antibody O/N at 4°C (5% FBS, 0.5% Tx, 0.2% gelatine in PBS; GFP (1:500, ThermoFisher Scientific). Slices were then repeatedly washed with PBS-0.5% Tx ...
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bioRxiv - Biochemistry 2022Quote: ... and heated at 95°C for 5 min before loading onto a NuPAGE Bis-Tris 4-12% gel (Invitrogen). Immunoblotting was performed using a Mini Trans-Blot cell (BioRad ...
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bioRxiv - Neuroscience 2020Quote: ... brains were transferred through 4 wells containing a blocking solution (5% Goat Serum in PBT; Life Technologies #16210-064), and sitting in the last well for 30 minutes ...
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bioRxiv - Cancer Biology 2020Quote: ... denaturated 5 min at 95°C and finally loaded on NuPAGE 4-12% Bis-Tris protein gels (Life Technologies) and electro-transferred onto nitrocellulose membranes ...
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bioRxiv - Molecular Biology 2021Quote: ... SDS-PAGE gels were loaded with 5 - 15 μL of lysate per lane (NuPAGE 4-12% Bis-Tris; Invitrogen). Lysates were diluted with additional 1x NuPAGE LDS buffer if necessary ...
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bioRxiv - Microbiology 2020Quote: ... and the wells were overlaid with 4 ml/well DMEM with 5% FBS and 0.7% agarose (Invitrogen, 16500-500). At 6 d.p.i. ...
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bioRxiv - Genomics 2021Quote: ... we extracted total RNA from rosette leaves of 4-5-week-old plants using Trizol (Invitrogen, cat. No. 15596026) according to manufacturer’s manual ...
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bioRxiv - Genomics 2021Quote: ... centrifuged at 500xg for 5 minutes at 4°C using a swinging bucket rotor (Sorvall Legend RT, Thermo Fisher), and then pellets were washed with an additional 1 mL cold lysis buffer and incubated on ice for an additional 5 minutes ...
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bioRxiv - Cancer Biology 2021Quote: ... which were boiled for 5 min at 95°C and then separated on 4-12% polyacrylamide gradient gels (Invitrogen). After blotting ...
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bioRxiv - Molecular Biology 2021Quote: ... A total of 5 μg protein was run on a NuPage™ 4-12% Bis-Tris Protein Gel (Invitrogen) using MOPS buffer according to manufacturer instructions and subsequently transferred to a nitrocellulose membrane according to the NuPage™ manufacturer's protocol (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... lysed in 1X Novex buffer and 5-7.5×105 cells equivalents were resolved on 4-12% NuPAGE gel (Invitrogen) in MOPS buffer ...
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bioRxiv - Neuroscience 2024Quote: ... 0.05% Tween-20) overnight at 4 °C and with secondary antibodies diluted in 5% nonfat milk in TBST (ThermoFisher, A16078 [goat anti- mouse HRP ...
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bioRxiv - Molecular Biology 2024Quote: ... Samples were heated to 80°C for 5 min and electrophoresed on 4-12% NuPAGE Bis-Tris gels (ThermoFisher). Wet transfer was to a nitrocellulose membrane (0.45 uM pore ...
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bioRxiv - Cell Biology 2024Quote: ... and aliquots containing 5 × 106 cells were separated on a NuPAGE bis-tris 4–12% gradient polyacrylamide gel (Invitrogen). The sample lane was divided into eight slices that were excised from the Coomassie-stained gel ...
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bioRxiv - Microbiology 2024Quote: ... were then mixed 1:1,000 with 5 mL of MMB agar + Tetracycline and poured into 4-well Nunc Rectangular Dishes (ThermoFisher). Dishes were dried at room temperature for 1 hour ...
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bioRxiv - Biochemistry 2023Quote: ... heated for 5 minutes at 95°C and loaded on 4-12% Bis-Tris NuPage gels (Thermo Fisher Scientific). After electrophoretic separation ...
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bioRxiv - Cell Biology 2023Quote: ... Primary antibody incubation was performed overnight at 4°C in PBST with 5% normal goat serum (Thermo Fisher, USA). Secondary antibodies were diluted in PBST and incubated for 1 hr at room temperature ...
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bioRxiv - Biochemistry 2023Quote: ... The established stable cells were cultured in Dulbecco’s modified Eagle’s medium (DMEM, Nacalai Tesque) supplemented with 4∼5% fetal calf serum (FCS; Thermo Fisher Scientific ...
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bioRxiv - Immunology 2023Quote: ... and cellular debris spun down at 12,000 g for 5 minutes before being subjected to electrophoresis on Bolt 4- 12% Bis-Tris gels from Invitrogen/Thermo Fisher (Cat ...
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bioRxiv - Developmental Biology 2023Quote: ... transgenic hg13 larvae (mmachchg13/hg13 Tg(col2a1a:EGFP)) were staged and fixed at 5 DPF with 4% paraformaldehyde (Fisher Scientific). Larvae were dissected for genotyping and unbiased imaging was performed on the remaining head tissue ...
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bioRxiv - Developmental Biology 2023Quote: ... Larvae were maintained in embryo media at 28.5°C until 5 DPF and fixed in 4% paraformaldehyde (Fisher Scientific) at room temperature for 1 hour and stored in 1x PBS (Fisher Scientific ...
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bioRxiv - Developmental Biology 2023Quote: ... Cells were subsequently centrifuged for 5 minutes at 200 x g and resuspended in 4% paraformaldehyde (Thermo Scientific Chemicals) for 30 minutes at 22°C ...
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bioRxiv - Neuroscience 2023Quote: ... Slices were rinsed with PBS (4 x 5 min) and mounted on microscope slides with ProLong Gold (ThermoFisher Scientific). Slices were kept in the fridge for at least two weeks before confocal imaging ...
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bioRxiv - Genetics 2023Quote: ... mice were overdosed with inhaled isoflurane (>5%) and transcardially perfused with 4°C Ca2+/Mg2+-free 1X HBSS (Invitrogen). Bilateral T11-L2 DRGs ...
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bioRxiv - Microbiology 2023Quote: ... 5′-labelled Aar RNA (4 nM) was incubated for 1 h at 37°C with 1× structure buffer (Ambion), 1 µg yeast RNA and putative mRNA interaction partners at the indicated concentrations (0 nM ...
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bioRxiv - Cell Biology 2023Quote: ... Lysates were clarified by centrifugation (18,253 xg, 5 min, 4°C) and protein concentrations determined by absorbance on a NanoDrop instrument (ThermoFisher). Equal amounts of lysate were mixed with 4x loading dye (2% SDS ...
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bioRxiv - Molecular Biology 2023Quote: ... SDS-PAGE gels were loaded with 5-15 μL of lysate per lane (NuPAGE 4-12% Bis-Tris; Invitrogen). Lysates were diluted with additional 1x NuPAGE LDS buffer if necessary ...