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Citations for Thermo Fisher :
4301 - 4350 of 5129 citations for MRPL54 Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... Peptide and enzyme solutions were dissolved and diluted in 25 mM 4-(2-hydroxyethyl)-1-piperazine ethanesulfonic acid (HEPES, Fisher Scientific). The peptide (100 μM in a total volume of 100 μL ...
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bioRxiv - Immunology 2021Quote: ... RNA-optimized Spike genes were designed based on the SARS-CoV-2 Spike protein sequence from Wuhan-Hu-1 (GenBank: MN_908947) with its original signal peptide and the DNAs were synthesized by GeneArt (Thermo Fisher Scientific). All Spike plasmids have the 2P stabilizing mutations by substituting of K986V987 [34–36] ...
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bioRxiv - Evolutionary Biology 2020Quote: ... About 1.5 micrograms of peptides from each sample were used for DIAMS measurement on an Orbitrap Fusion Lumos Tribrid mass spectrometer (Thermo Scientific) platform coupled to a nanoelectrospray ion source ...
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bioRxiv - Systems Biology 2020Quote: ... Single U-937 monocyte and macrophage cells were isolated and distributed into 1μl of pure water with MassPREP peptide mixture (25fmol/μl final concentration, Waters 186002337) in 384-well PCR plates (ThermoFisher AB1384) using a BD FACSAria I cell sorter ...
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bioRxiv - Biochemistry 2021Quote: ... Peptides were analyzed by LC-MS/MS using a data-independent acquisition method as described previously.69 Extracted ion chromatograms for fragment ions derived from tryptic peptides were plotted using Xcalibur Qual Browser (Thermo Scientific). The area under the curve for each peak was used for quantification.
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bioRxiv - Physiology 2020Quote: ... 50 μg of peptides from each sample were labelled with 5 μl of Tandem Mass Tag 10-plex (TMT, Thermo Scientific) reagent (0.02mg/μl ...
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bioRxiv - Systems Biology 2020Quote: ... peptides were fractionated at pH 10 with a ‘spider fractionator’ coupled to an EASY-nLC 1000 chromatography system (Thermo Fisher Scientific) as described previously31 ...
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bioRxiv - Biochemistry 2020Quote: ... The liquid was evaporated to dryness and the peptides were separated on an EASY-spray column connected to an EASY-nLC 1000 system (Thermo Scientific). The peptides were eluted in a 60 min gradient (from 5-26% of buffer B (2% acetonitrile ...
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bioRxiv - Biochemistry 2021Quote: ... were centrifuged at 16000 g for 30 min and the concentration of peptides were measured by Nanodrop one Microvolume UV-Vis Spectrophotometer (Thermo Fisher). All peptide samples were analyzed in technical triplicate by LC-MS using an Easy-nano LC 1200 system coupled to an Orbitrap Fusion Lumos mass spectrometer (Thermo Fisher) ...
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bioRxiv - Biochemistry 2020Quote: ... the theoretical m/z values for all peptides were used to interrogate the raw data using the Quan Browser software program (Thermo Scientific) (Table 1) ...
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bioRxiv - Cancer Biology 2021Quote: ... After desalting using C18 Stage tips extracted peptides were separated on an Easy-nLC 1200 system coupled to a Q Exactive HF mass spectrometer (both Thermo Scientific) as described elsewhere by Kliza et al (22 ...
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bioRxiv - Biochemistry 2021Quote: ... The digested elutions samples were reconstituted with 0.1% trifluoroacetic acid (TFA) in LC-MS grade water and loaded onto the Pierce peptide desalting spin columns (Thermo Fisher Scientific) according to the manufacturer’s protocols ...
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bioRxiv - Biochemistry 2020Quote: Processed peptides were measured by reversed phase liquid chromatography-tandem mass spectrometry (LC-MS/MS) on an EASY nLC1000 (Thermo Scientific) coupled to a quadrupole orbitrap mass spectrometer (Q-Exactive ...
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bioRxiv - Biochemistry 2020Quote: ... the valve switched to allow peptides to be separated on an Acclaim PepMap C18 nano column (3 µm, 75µm x 25cm, Thermo Fisher Scientific), in a 120 min gradient of 5% to 40% B at 300 nL/min ...
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bioRxiv - Microbiology 2020Quote: ... Twelve micrograms of peptides were labelled with TMT labels (6-plex) according to the manufacturer’s instructions (Thermo Fisher Scientific, Waltham, MA) and pooled ...
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bioRxiv - Microbiology 2020Quote: ... The resulting tryptic peptides were dried by speed vacuum at 4 °C and desalted with a C18 spin column (Thermo Scientific) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2020Quote: ... Peptide mixtures were loaded onto a PepMap 100 C18 trap column (75 μm id x 2 cm length; Thermo Fisher Scientific) at 3 μL/min for 10 min with 2 % acetonitrile (volume fraction ...
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bioRxiv - Microbiology 2021Quote: ... Protein samples were trypsin digested and peptide mixtures were analyzed by a Q-Exactive mass spectrometer coupled to an Easy-nLC system (both from Thermo Scientific). All MS/MS data were processed with Proteome Discoverer 2.1 (Thermo Scientific ...
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bioRxiv - Microbiology 2021Quote: ... The resulting peptides were analyzed by online nanoliquid chromatography coupled to tandem MS (Ultimate 3000 RSLCnano and Q-Exactive HF, Thermo Scientific). Peptides were sampled on a 300 μm × 5 mm PepMap C18 precolumn (Thermo Scientific ...
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bioRxiv - Immunology 2020Quote: ... samples were proteolysed by a modified filter aided sample preparation 57 and eluted peptides were analysed by LC-MSMS on a QExactive HF mass spectrometer (ThermoFisher Scientific) coupled directly to a UItimate 3000 RSLC nano-HPLC (Dionex) ...
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bioRxiv - Immunology 2021Quote: Mass spectrometry data acquisition: Analysis of TMT labeled peptide digests was carried out on an Orbitrap Q Exactive HF-X instrument from Thermo Fisher Scientific (Bremen ...
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bioRxiv - Cell Biology 2021Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min organic gradient ...
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bioRxiv - Genetics 2019Quote: ... Approximately 1 μg of peptides from each digested band was analysed using LC-MS/MS on a Q Exactive mass spectrometer (ThermoFisher Scientific), as previously described [66].
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bioRxiv - Genetics 2020Quote: ... The resulting peptides were fractionated using a Dionex Ultimate 3000 nanoHPLC system in line with an LTQ-Orbitrap Velos mass spectrometer (Thermo Scientific). In brief ...
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bioRxiv - Biophysics 2021Quote: ... SARS-CoV-2 ORF10 protein (full lenth, crude, Mass spectrometry data of crude peptides is included in supplementary information) was purchased from Thermo Scientific, USA.
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bioRxiv - Cancer Biology 2020Quote: ... The second half of the data (runs 5-8) was obtained after peptide separation on 75 μm columns packed with 2.6 μm Accucore beads (Thermo Fisher Scientific). Peptides were injected onto 30-40 cm ...
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bioRxiv - Immunology 2022Quote: ... The concentrated peptides were injected into in a Q Exactive HFX instrument (Thermo-Scientific) connected to an Easy-nLC 1200 instrument (Thermo Scientific). The peptides were analyzed in data-dependent mass-spectrometry (DDA-MS ...
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An Azobenzene G-quadruplex Ligand Exhibits Promising Antibacterial Activity against Escherichia colibioRxiv - Microbiology 2022Quote: ... acetonitrile 0.1% (vol/vol) formic acid peptides were resolved on a 250 mm × 75 μm Acclaim PepMap C18 reverse phase analytical column (Thermo Scientific) over a 150 min acetonitrile gradient ...
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bioRxiv - Neuroscience 2022Quote: ... Eluted peptides were dried down overnight before resuspension in 5% formic acid ready for injection onto the Orbitrap Exploris (Thermo Fisher) mass spectrometer using data independent acquisition (DIA) ...
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bioRxiv - Biochemistry 2022Quote: ... Mass recalibration for each sample was determined manually by performing a separate search on each raw file for linear peptides in Proteome Discoverer 2.4 (Thermo Fisher Scientific) without spectrum recalibration ...
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bioRxiv - Biophysics 2022Quote: ... faecalis was purified as described previously55 and fluorescently labeled at the stem peptide lysine with Alexa Fluor 488 NHS ester (Life Technologies). For labeling ...
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bioRxiv - Cancer Biology 2022Quote: ... Dried peptides were dissolved in 0.1% TFA and analysed by LC-MS/MS on Q Exactive plus mass spectrometer (Thermo Fisher Scientific). Peptide identification was performed using the Mascot search engine (Casado and Cutillas ...
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bioRxiv - Biophysics 2022Quote: ... The cross-linked peptides were separated on a PepMap RSLC column (250 mm × 75 μm, 2 μm particle size, ThermoFisher Scientific). Chromatographic separation was performed with a 60 min gradient starting at 6% and increasing to 40% B (mobile phase A ...
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bioRxiv - Cancer Biology 2022Quote: Peptide samples were analyzed on a Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass Spectrometer (Thermo Fisher Scientific, Rockford, IL, USA) coupled with a high-performance liquid chromatography system (EASY nLC 1200 ...
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bioRxiv - Biochemistry 2022Quote: ... Synthetic peptides were coupled to rabbit serum albumin (RSA) with the linker Sulfo-m-maleimidobenzoyl-N-hydroxysuccinimide ester (Sulfo-MBS, Thermo Scientific) in a carrier (1):linker (50):peptide (50 ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting peptides were collected on a trap column (2 mm x 2 cm) filled with POROS 20 R2 material (Thermo Scientific) kept at 0.5 °C ...
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bioRxiv - Biophysics 2022Quote: ... Peptides were directly injected over the course of a 75 min acquisition into a Q-Exactive Plus mass spectrometer (ThermoFisher Scientific). Raw MS data were searched against the Uniprot canonical isoforms of the human proteome using the default settings in MaxQuant (version 1.6.12.0) ...
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bioRxiv - Cancer Biology 2022Quote: The resulting peptides were analyzed using a nanoAcquity liquid chromatography (Waters) coupled with a Q Exactive HF-X (Thermo fisher scientific). Samples were analyzed randomly ...
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bioRxiv - Cell Biology 2022Quote: ... 1,000 ng of peptides from each biological replicate were first injected onto an using an Ultimate3000 high-performance liquid chromatography system (ThermoFisher Scientific) coupled online to an Orbitrap HF-X (ThermoFisher Scientific) ...
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bioRxiv - Developmental Biology 2022Quote: ... Digested peptides were diluted to 60 μL with water and labeled for 1 hour at room temperature using 16plex TMTpro (Thermo Scientific) or 10plex TMT (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... Eluted peptides were introduced into the mass spectrometer (Q Exactive™ Hybrid Quadrupole-Orbitrap™ Mass Spectrometer, Thermo Fisher Scientific, USA). The spray voltage was set at 2.2 kV and the heated capillary at 270°C ...
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bioRxiv - Cell Biology 2022Quote: ... Peptides eluted from the capillary column were applied directly onto a Q Exactive Plus mass spectrometer by electrospray (Thermo Fisher Scientific) for mass spectrometry (MS ...
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bioRxiv - Immunology 2022Quote: ... The sites of phosphorylation of 32P-labeled peptides were also determined by solid-phase Edman degradation on a Shimadzu PPSQ33A Sequencer (Kyoto, Japan) with the peptides coupled to an 80 Sequelon-AA membrane (Applied Biosystems), as described previously (Campbell and Morrice ...
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bioRxiv - Plant Biology 2022Quote: ... The peptides were initially trapped on a C18 PepMap100 pre-column (300 µm i.d. x 5 mm, 100 Å, Thermo Fisher) using solvent A (0.1% formic acid in water) ...
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bioRxiv - Microbiology 2022Quote: ... and the resulting peptides were collected on a trap column (2 mm × 2 cm) with POROS 20 R2 material (Thermo Scientific) at 0.5°C ...
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bioRxiv - Microbiology 2022Quote: The raw LC-MS/MS data was analyzed against the Uniprot Gallid alphaherpesvirus 2 database (taxon 10390; 1300 sequences) using the Byonic peptide search algorithm (Protein Metrics) integrated into Proteome Discoverer 2.4 (Thermo Fisher Scientific). Optimal main search settings were initially determined with Byonic Preview (Protein Metrics ...
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bioRxiv - Microbiology 2022Quote: ... The peptides were analyzed by capillary LC-tandem mass spectrometry using an Orbitrap Fusion Lumos tribrid ion trap mass spectrometer (ThermoFisher Scientific). The resulting MS/MS data was processed using Sequest HT search engine within Proteome Discoverer 2.5 SP1 against a Homo Sapiens protein database obtained from Uniprot (78 787 entries ...
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bioRxiv - Plant Biology 2022Quote: ... Peptides were loaded onto a trapping microcolumn Acclaim PepMap100 C18 (20 mm x 100 μm ID, 5 μm, 100Å, Thermo Scientific) before separation on a reversed-phase custom packed nanocolumn (75 μm ID × 40 cm ...
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bioRxiv - Molecular Biology 2023Quote: Separation of peptides produced by AspN digestion was performed on a ICS3500 nano-UHPLC system (Thermo Fisher Scientific/Dionex, Germering, DE)) employing a 75 μm x 150 mm Easy spray column packed with C-18 reverse phase (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2023Quote: The peptides were analyzed by liquid chromatography (LC)-MS/MS (PROXEON coupled to a QExactive HF mass spectrometer, Thermo Fisher Scientific) with three injections of 5 μl digests ...