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Citations for Thermo Fisher :
4401 - 4450 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... and the same number of cells were re-plated in 6-well plates and selected with G418 (800 µg/ml) (11811031, Thermo Fisher scientific) for 8-14 days ...
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bioRxiv - Neuroscience 2024Quote: ... iNeurons were resuspended and plated at 600K/well in PLO-lam coated 6-well plates in Neurobasal medium (NBM; Fisher Scientific, 11570556), supplemented with 200 mM GlutaMAX (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... Brain extracts containing 60 ug of protein were loaded and separated on 6-10% denaturing polyacrylamide gel electrophoresis (SDS-PAGE) and transferred to polyvinylidene difluoride (PVDF) membrane (Cat#88518; Thermo Fisher Scientific). The membrane was blocked with 5% milk in Tris-buffered saline with 0.1% Tween (TBST ...
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bioRxiv - Biochemistry 2024Quote: ... the cell debris was sedimented using ultracentrifugation at 100000xg (SorvallTM WX+, Thermo Scientific with A-27 fixed angle rotor 6 x 50mL). The supernatant was then filtered using 0.2 nm syringe cap filters ...
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bioRxiv - Molecular Biology 2024Quote: ... of samples were pooled and separated into 6 fractions by off-line basic reversed-phase (bRP) using the Pierce High pH Reversed-Phase Peptide Fractionation Kit (Thermo Fisher Scientific). The fractions were collected in 7.5 ...
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bioRxiv - Microbiology 2024Quote: ... Samples were concentrated onto the trap column at 5 µl/min for 6 min with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap 480™ (Thermo Fisher Scientific) at 300 nl/minute via the analytical column ...
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bioRxiv - Immunology 2024Quote: ... Real-time PCR was performed using SYBR Green Realtime PCR Master Mix (TOYOBO) on ABI QuantStudio 6 flex Real-time PCR System (Thermo Fisher Scientific). The RT-qPCR Primer sequences for targeting respective genes are displayed in Table S1 ...
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bioRxiv - Immunology 2024Quote: ... Cleaned skin was then cut in small pieces with scissors and digested in 6 ml of CO2 independent medium (Gibco 18045-054) containing Hyaluronidase type IS 500 µg/ml (Sigma-Aldrich H3506) ...
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bioRxiv - Immunology 2024Quote: ... qPCR was performed using a PowerUp SYBR Green Master Mix and standard protocols on an Applied Biosystems QuantStudio 6 Real-Time PCR System (Thermo Fisher Scientific). RPL19 was used to normalize RNA isolated from mouse samples ...
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bioRxiv - Molecular Biology 2024Quote: ... An additional size-fractionation step was performed for all EV and NVEP fractions using a Novex TBE PAGE gel (6%, Invitrogen, Cat# EC6265BOX) with a size range of 150 – 400 bp for cell line-derived cells and EVs ...
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bioRxiv - Microbiology 2024Quote: ... Samples were concentrated onto the trap column at 5 µl/minutes for 6 minutes with Buffer A (0.1% formic acid, 2% DMSO) and then infused into an Orbitrap 480™ (Thermo Fisher Scientific) at 300 nl/minute via the analytical columns ...
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bioRxiv - Cancer Biology 2024Quote: Twenty-five thousand MDA-231 or D2A1 cells were seeded in the wells of a 6-well plate and cultured in Phenol free DMEM media (ThermoFisher, catalog# 31053028) supplemented with 10% (v/v ...
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bioRxiv - Molecular Biology 2024Quote: ... or 2.5 µg (6-well plates) of plasmid DNA diluted in OptiMEM medium using the Lipofectamine 3000 reagent (Invitrogen, ThermoFisher Scientific, France) for 48 h ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was adjusted to pH 5.8 using 1N KOH prior to addition of 6 g/L Phytagar (Gibco, Life Technologies, U.S.A) and autoclaving for 20 min at 121°C ...
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bioRxiv - Neuroscience 2022Quote: ... Culture 1 1% (Gibco), fetal bovine serum 2.5% and 2 μg/ml doxycycline hydrochloride.
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bioRxiv - Cancer Biology 2022Quote: ... mixed 1:1 with addition of 1% B27 (Invitrogen), 0.5% N2 (Invitrogen) ...
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bioRxiv - Microbiology 2023Quote: ... treated 1:1 with chloroform (Fisher Scientific, C606-1) for 15 minutes ...
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bioRxiv - Immunology 2021Quote: ... Mice were genotyped by PCR using forward primers 5’-ctgagcagagacccactgaaag-3’ and reverse primers 5’- ggatctggcttctgagtttgtgta-3’ and amplicons were ran in 6% TBE gels (Life Technologies, Carlsbad, CA).
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bioRxiv - Immunology 2021Quote: Sigma Aldrich’s CHOZN cells were cultured in EX-CELL CD CHO Fusion medium (MilliporeSigma, Burlington, MA, USA) supplemented with 6 mM GlutaMAX (Gibco, Waltham, MA, USA), shaking at 37°C ...
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bioRxiv - Microbiology 2020Quote: Lysates of infected HeLa cells were prepared by scraping three wells of a 6-well plate into 300-500 μL volume of ice-cold Mammalian Protein Extraction Reagent (M-PER™) buffer (Thermo Fisher Scientific) supplemented with cOmplete™ Mini EDTA-free Protease Inhibitor Cocktail (Roche ...
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bioRxiv - Microbiology 2019Quote: ... ATPase was assayed by a continuous spectrophotometric method using a 2-amino-6-mercapto-7-methylpurine ribonucleoside/purine nucleoside phosphorylase reaction to detect the released inorganic phosphate (EnzChek Kit; Life Technologies, California, USA) (53) ...
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bioRxiv - Molecular Biology 2021Quote: ... A similar protocol was followed for small scale virus production when establishing individual stable cell lines with transfection being performed on 6-well plates using Lipofectamine 2000 (Thermo Fisher Scientific, 11668019) reagent.
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bioRxiv - Immunology 2022Quote: ... containing 100 µl of tempus RNA- stabilizing solution aliquoted from a regular-sized tempus tube (designed for the collection of 3 ml of blood and containing 6 ml of solution; ThermoFisher, Waltham, MA, USA). This method is described in detail in an earlier report (7) ...
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bioRxiv - Neuroscience 2021Quote: ... Multiplexing quantification used the TMT-128 and TMT-131 channels from a 6-plexing tandem mass tag (TMT) kit (Thermo Fisher, Waltham, MA). See details in the SI document.
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bioRxiv - Neuroscience 2021Quote: ... Fish were euthanized 6 hours post-treatment and Biocytin was histologically detected using Alexa Fluor 594-conjugated Streptavidin (Molecular Probes, cat# S-11227). Biocytin-labeled axons were quantified using the “threshold” and “particle analysis” tools in the Fiji software ...
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bioRxiv - Molecular Biology 2020Quote: ... Samples with RIN≥6 were processed for microarray as described (19) and hybridized onto Affymetrix HT HG-U133PM+ arrays (Affymetrix, Santa Clara, CA) using a GeneTitanR according to Affymetrix technical protocols ...
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) reactions were carried out using the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, Life Technologies) with the SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Immunology 2022Quote: BMDMs obtained by standard procedure were seeded into 6-well plates (1X106 cells per well) in DMEM with 10% dialyzed FBS (Thermo Scientific, Waltham, MA), 4 mM glutamine ...
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bioRxiv - Genomics 2020Quote: Small-interfering (si)RNA knockdown of LOC157273 was performed on primary human hepatocytes from donor Hu8200 (genotype G/G at rs4841132) plated into collagen-coated 6-well plates (ThermoFisher Scientific #A11428-01). Cells were incubated in complete Williams’ E medium and transfected with siRNA (50 nM ...
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bioRxiv - Genomics 2020Quote: Motility agar plates were prepared by adding 20 mL of BHI with 0.3% agar per well of a Corning Costar 6-well plate (Thermo Fisher Scientific, Waltham, MA). C ...
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bioRxiv - Molecular Biology 2021Quote: ... Quantitative PCR was performed on a QuantStudio 6 Flex Real-Time PCR System using SYBR Green Power SYBR™ Green PCR-Master-Mix (Applied Biosystems™) and primers listed in Supplementary Table 2.
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bioRxiv - Evolutionary Biology 2019Quote: ... and 6 μl of DEPC-treated water using the SuperScript™ III First-Strand Synthesis System for RT-PCR (Invitrogen, Carlsbad, CA, US). Amplification products were visualized in a 2% agarose gel and photographed under the same parameters for each gene.
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bioRxiv - Cancer Biology 2019Quote: Transfections were performed of cells seeded in 6-well plates using DharmaFECT1 as per manufacturer’s protocol (Dharmacon; Thermo Fisher Scientific Inc., Waltham, MA). DUSP4 (M-003963-03 ...
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bioRxiv - Cell Biology 2020Quote: 2 × 105 cells were transfected in a gelatin-coated 6-well plate in ES DMEM-2i using Lipofectamine 2000 (Fisher Scientific, 11668-027). Two pairs of gRNA were used for each gene (1 µg each ...
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bioRxiv - Developmental Biology 2019Quote: ... The samples were systematically sectioned (6 μm thickness) using a rotary microtome (Microm HM355S, MICROM International GmbH, Germany) and were mounted on Polysine®slides (Thermo Fisher Scientific). Slides were stored at 4 °C for a maximum of one week before use.
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bioRxiv - Cell Biology 2021Quote: Following a 90 min feed with 5% chicken egg yolk, guts were dissected from larvae (6 dpf, 10 guts pooled per sample) and stored in RNAlater (Thermo Fisher Scientific AM7020) at 4°C for 1 week ...
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bioRxiv - Biochemistry 2021Quote: ... A673 cells (5.0 × 105) in 6-well dishes were reverse transfected with siRNA (50 nM) using Lipofectamine™ RNAiMAX (Invitrogen, cat. no.13778075) according to manufacturer’s instructions ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, Thermo Fisher SCIENTIFIC #F9-6 x 1000 LEX rotor). Cells were washed once with water and suspended into 75 mL ice cold water ...
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bioRxiv - Biochemistry 2020Quote: ... Cells were pelleted down by centrifugation (10628 g at 25°C for 5 min, Thermo Fisher SCIENTIFIC #F9-6 x 1000 LEX rotor). The cells were washed once with sterilized water and re-suspended into 6 L MM composed of 1.34% yeast nitrogen base without amino acids (SIGMA Y0626) ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Neuroscience 2021Quote: ... The nuclei of astrocytes and autaptic neurons were visualized by counterstaining with 4’,6-diamidino-2-phenylindole (DAPI)-containing mounting medium (ProLong® Gold Antifade Reagent with DAPI, Thermo Fisher Scientific). Only neurons with one nucleus were analyzed when counting synapse numbers.
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bioRxiv - Cell Biology 2021Quote: ... The generated cDNA was used for real-time quantitative PCR using the SYBR Green Master mix in a QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher Scientific, 4309155) using the primers listed in Table S1 ...
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bioRxiv - Plant Biology 2021Quote: ... The PCR reaction was performed in a QuantStudio™ 6 Flex Real-Time PCR system instrument (384-well, Applied Biosystems, Foster City, CA) with an initial incubation at 95°C for 10 min ...
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bioRxiv - Neuroscience 2020Quote: ... then replenished with either 200 μL (chamber slide cells) or 6 mL (150 mm dish cells) of Opti-MEM I (ThermoFisher Scientific, Cat#31985062). BioPORTER medium mix was added at a 1:1 volume ratio in the cells grown in chamber slides and a 1:3 volume ratio in the cells grown in 150 mm dishes ...
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bioRxiv - Microbiology 2021Quote: Keratinocytes were seeded at a density of 5×105 cells/well in a 6-well tissue culture plate (Nunc; Thermo Fisher Scientific, Singapore) and grown for 3 days at 37°C in a 5% CO2 humidified atmosphere ...
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bioRxiv - Molecular Biology 2021Quote: ... 0.2 μg of each pmirGLO construct was transfected into human HEK-293T cells [44] (6 × 104 cells/well) with 1.6 pmol of miR-155 or scrambled control miRNA (Thermo Scientific Dharmacon, Waltham, MA) in a 96-well plate ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then counted and 500 cells per treatment were plated in triplicate in 6 well plates (Nunc, Thermo Fisher, UK, Cat: 10119831) and incubated for 12 days ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were then counted and 500 cells per treatment were plated in triplicate in 6 well plates (Nunc, Thermo Fisher, UK, Cat: 10119831) and incubated for 12 days ...
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bioRxiv - Cell Biology 2021Quote: ... coverslips were washed once with PBS and before mounting on objective glass with 6% n-propyl gallate diluted in glycerol and 10x PBS and combined with ShandonTM Immuno-MountTM (Thermo Scientific, cat# 9990402) in a 1:12 ratio ...
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bioRxiv - Cell Biology 2020Quote: ... 3 µl of siRNA or 6 µl RNAiMAX were each mixed with 125 µl of Gibco Roswell Park Memorial Institute (RPMI) 1640 Medium (11875093, Thermo Fisher Scientific Inc.) and incubated separately at room temperature for 2 min ...