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Citations for Thermo Fisher :
4551 - 4600 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2024Quote: ... tissues from 6 different zebrafish were pooled in each condition and we used TriZol protocol to extract total RNA (Invitrogen, Carlsbad, California, USA). The protocol conditions for sample preparation and quantification have been previously detailed (Seiliez and al ...
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bioRxiv - Neuroscience 2024Quote: ... After incubation with the secondary antibody sections were washed three times for 6 min in PBS and mounted on microscopic slides covered with mounting dye with DAPI (Invitrogen, 00-4959-52).
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bioRxiv - Cell Biology 2024Quote: ... RPE1 cells stably expressing dCas9-GFP-3xFKBP were seeded in 6 well plates (120 000 cells/well, Thermo Fisher Scientific, Waltham, MA, USA) the day before lentivirus transduction ...
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bioRxiv - Bioengineering 2024Quote: ... 25 µg of proteins were separated in each well using 6% sodium dodecyl sulfate-polyacrylamide gel electrophoresis gel with pre-stained molecular weight markers (ThermoFisher St. Louis, MO) and transferred to PVDF (polyvinylidene ...
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bioRxiv - Bioengineering 2024Quote: ... were complexed for 20 minutes with 6 μL of TransIT-X2 in 50 μL of Opti-MEM™ Reduced Serum Medium (Gibco, Cat# 31985062). After complexing ...
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bioRxiv - Cell Biology 2024Quote: ... 15 000 cells/cm2 were plated in a 6-wells plate and after one day transfected using Lipofectamine 2000 (Thermofisher, Cat no. 11668030) in OptiMEM (Gibco ...
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bioRxiv - Biophysics 2020Quote: ... The concentrations of cdM2-1 and RNA were determined by absorbance at 280 and 260 nm using extinction coefficients of 5,960 M−1·cm−1 (Expasy-ProtParam) (14) and 8,050 M−1·cm−1·base−1 (ThermoFisher Scientific ...
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bioRxiv - Biophysics 2019Quote: ... ZO-1 (1:100, ThermoFisher), vimentin (1:100 ...
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bioRxiv - Bioengineering 2020Quote: ... 1:1,(GIBCO, Life Technologies) medium supplemented with 10% fetal bovine serum(GIBCO) ...
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bioRxiv - Bioengineering 2020Quote: ... 1:1,(GIBCO, Life Technologies) medium supplemented with 10% fetal bovine serum(GIBCO) ...
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bioRxiv - Neuroscience 2019Quote: ... 1:1 Neurobasal (ThermoFisher; 21103049) with 1× Glutamax ...
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bioRxiv - Cell Biology 2023Quote: ... 1% HEPES (1 M, Gibco), 1% L-glutamine (200 mM ...
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bioRxiv - Cell Biology 2022Quote: ... 1% HEPES 1 M (Gibco), 1% Sodium Pyruvate 100 mM (Gibco) ...
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bioRxiv - Cell Biology 2023Quote: ... ZO-1 (1:100, ThermoFisher), E-Cadherin (1:100 ...
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bioRxiv - Microbiology 2020Quote: ... and 1:1 3-methyl-1-butanol (Thermo Fisher Scientific) in mineral oil were used as cues ...
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bioRxiv - Developmental Biology 2022Quote: ... and 1% antibiotics in DMEM/F12 (1:1) media (Gibco).
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bioRxiv - Immunology 2022Quote: ... Zonula occludens-1 (ZO-1, 1:250 Invitrogen 33-9100), and Cav-1 (1:500 Invitrogen PA5-17447) ...
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bioRxiv - Neuroscience 2024Quote: ... cells were switched to neural induction media (DMEM/F12 and Neurobasal medium at 1:1 ratio, 1% B27, 0.5% N2, 1% PenStrep, 1% Glutamax; ThermoFisher Scientific) supplemented with ascorbic acid (0.1 mM ...
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bioRxiv - Plant Biology 2020Quote: ... qRT-PCR was performed with 2x PowerUP™ SYBR™Green Master Mix in the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems). GAPDH (AT1G13440 ...
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bioRxiv - Microbiology 2020Quote: Cells in 6- and 12-well format were washed with PBS and treated with 10 mM TrypLE™ Express Enzyme (Gibco; Cat. No. 12605028). Cells were then collected with PBS and added to 10% PFA to a final concentration of 4% ...
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bioRxiv - Cell Biology 2020Quote: ... Harvested equine explants were allowed to recover in 6-well plates for 3-7 days in the following control medium: DMEM/F12 medium (11330, Thermo Fisher Scientific, Waltham, MA), 10% fetal bovine serum (Seradigm 1500-500 ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Molecular Biology 2020Quote: ... Quantitative real-time PCR (qRT-PCR) reactions were carried out using the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, Life Technologies) with the SYBR Green PCR Master Mix (Applied Biosystems ...
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bioRxiv - Microbiology 2021Quote: ... 105 rabbit Sf1 epithelial cells were plated into each well of a 6-well plate and incubated at 37°C overnight in a 5% CO2 atmosphere within a HeraCell 150 incubator (Thermo Fisher Scientific, Waltham, MA). At the same time ...
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bioRxiv - Immunology 2021Quote: Human T cells were differentiated for 6–7 days in a humidified 5% CO2 incubator at 37°C with IMDM (Thermo Fisher, Waltham, MA, USA) supplemented with 10% fetal bovine serum ...
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Redundant and specific roles of cohesin STAG subunits in chromatin looping and transcription controlbioRxiv - Cell Biology 2019Quote: ... The cells are then transferred with warm hNSC medium to a 6-well plate coated with Geltrex (Gibco, A1413202, incubate for 1hr at 37C). The day after transfection the medium was refreshed and 1µg/ml puromycin (Sigma ...
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bioRxiv - Neuroscience 2019Quote: ... ∼25000 astrocytes were plated per 30 mm glass coverslip in 6-well plates and allowed to form micro-dot islands in DMEM (Thermo Fisher Scientific, Cat #11965092) supplemented with 10% (vol/vol ...
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bioRxiv - Cancer Biology 2020Quote: ... MiRNAs or vectors were transfected in the amount of 2 μg for 6-well plate into indicated cells by Lipofectamine 2000 transfection reagent (Thermo Fisher Scientific, Waltham, USA) according to the manufacturer’s instructions ...
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bioRxiv - Pathology 2020Quote: ... Quantitative reverse transcriptase-polymerase chain reaction (qRT-PCR) analysis of interleukin (IL)-6 was performed using TaqMan Gene Expression Assays (Rn01410330_m1; Applied Biosystems, Foster City, CA, USA) and 7900HT Fast Real-Time PCR System (Applied Biosystems) ...
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bioRxiv - Biophysics 2019Quote: ... Bacteria were harvested by centrifugation for 10 minutes at 4800 rpm (Thermo Lynx 4000 F12-6 rotor, Thermo Fisher Scientific, Waltham, MA, United States). The obtained pellets were stored at −80 °C until needed ...
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bioRxiv - Biochemistry 2020Quote: ... Insoluble fraction was removed by high speed centrifugation at 4°C (25658 g for 30 minutes, Thermo Fisher SCIENTIFIC #A23-6 x 100 rotor). The supernatant was filtrated with 0.22 μm filter (Filtropur BT50 0.2 ...
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bioRxiv - Bioengineering 2020Quote: ... the coverslips were placed upside down on a glass slide with slow fade mounting medium containing 4’,6-diamidino-2- phenylindole (DAPI, Life Technologies, Carlsbad, CA, USA) and kept at 4 °C until observation ...
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bioRxiv - Cancer Biology 2021Quote: ... the 5’ end of the forward primer sequences for RheBAT26 and RheD18S58 MSI markers was labeled with a 6-FAM fluorescent dye (Thermo Fisher Scientific, Waltham, MA). A multiplex PCR was designed to amplify RheBAT26 and RheD18S58 MSI markers with labeled primer sequences ...
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bioRxiv - Plant Biology 2020Quote: ... 20 ng of reverse transcribed RNA was used as template in the real-time PCR using Applied Biosystems(tm) QuantStudio(tm) 6 Flex (Fisher Scientific GmbH, Schwerte, Germany). Specific primers binding the 3’-UTR of the NOS terminator were used for the detection of the transgene transcripts ...
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bioRxiv - Microbiology 2020Quote: ... were acid digested on a hot plate using 6 mL Primar Plus grade HNO3 (68%) and 2 mL H2O2 (Thermo Fisher Scientific, Loughborough, UK). Samples were diluted with Milli-Q water (18.2 MΩ cm ...
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bioRxiv - Molecular Biology 2020Quote: ... Real-time qPCR reactions were carried on using power up SYBR green master mix on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, Life Technologies Inc.). Ct (cycle threshold ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated at 37°C in normoxia (21% O2) for Balb/c MSC or hypoxia (5% O2) for C57BL/6 MSC in culture medium consisting of MEM-α (ThermoFisher Scientific, Dublin, Ireland) supplemented with 10% heat-inactivated foetal bovine serum (FBS ...
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bioRxiv - Molecular Biology 2020Quote: ... wound lysate cell suspensions were sorted from Tie-2Cre/CXCL12loxP/loxP and CXCL12loxP/loxP transgenic mice as single progenitor cells into each well of a 96-well plate using a Becton Dickinson FACSAria flow cytometer (Franklin Lakes, NJ) into 6 μl of lysis buffer and SUPERase-In RNAse inhibitor (Applied Biosystems, Foster City, CA) (N=5 mice) ...
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bioRxiv - Cell Biology 2021Quote: ... Quantitative real-time PCR was performed in triplicates using the QuantStudio 6 Flex Real-Time PCR System and Taqman Universal PCR Master Mix (Applied Biosystems, Foster City, CA). Probe sets for Il6 (Rn01410330_m1) ...
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FGF10 triggers de novo alveologenesis in a BPD model: impact on the resident mesenchymal niche cellsbioRxiv - Cell Biology 2022Quote: ... at RT and washed three times with TBST before being mounted with Prolong Diamond Anti-fade Mountant with DAPI (Invitrogen 4’,6-diamidino2-phenylindole). Photomicrographs of immunofluorescence staining were taken using a Leica DMRA fluorescence microscope with a Leica DFC360 FX camera (Leica ...
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bioRxiv - Cancer Biology 2022Quote: ... 200’000 cells of selected monocolonies of either cell line were seeded in 6-well plates and transfected on next day with CRISPR plasmids using Lipofectamine 2000 (Invitrogen by Life Technologies #11668019) according to manufacturer instructions ...
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bioRxiv - Biochemistry 2022Quote: ... The sample was blotted for 25 s with a force of 6 and then flash-frozen in liquid ethane and stored in liquid nitrogen using a Vitrobot Mark IV (Thermo Fisher Scientific, Waltham, USA) with the environmental chamber set at 100% humidity ...
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bioRxiv - Biophysics 2022Quote: Human adipose-derived stem cells (hASCs, passage p<=6) were obtained from the Radboud Biobank and cultured in Minimum Essential Medium Eagle (α-MEM) (Invitrogen, Thermo Fisher, USA). All media were supplemented with 10% fetal bovine serum (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2022Quote: ... 1.6 μL of the RNA extracts were used for RNA measurement with the use of the NanoDrop™ 2000 Spectrophotometer (Thermo Fisher Scientific, UK). The RNA samples were then stored at -80 °C.
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bioRxiv - Microbiology 2020Quote: ... Then RNA was reverse transcribed into cDNA and the transcriptional levels of different genes were determined by qRT-PCR using SybrGreen Premix Low rox (MDbio, China) and the QuantStudio™ 6 Flex (Thermo Fisher Scientific, America). 16S rRNA was used as an internal reference and the merF gene expression was calculated using the 2−ΔΔCt method ...
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bioRxiv - Microbiology 2021Quote: After the skin flap surgery and 15 min prior to the tail-vein injection of GFP-expressing bacteria (107 CFU in 100 μl 1x PBS) and PE-conjugated anti-human CD62E (6 μg, Thermofisher Scientific, #12-0627-42), 100 μg Dylight755-conjugated UEA-1 lectin (Unconjugated UEA-1 lectin ...
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bioRxiv - Molecular Biology 2022Quote: ... HEK293T cells were seeded into 6 well plates and transfected with 1ug plasmid mixture of pX459-gRNA1 and pX459-gRNA2 using Lipofectamine 2000 (Thermo Fisher Scientific, Cat. 11668019). On the next day ...
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bioRxiv - Cell Biology 2022Quote: ... Library quantification was performed using the Collibri™ Library Quantification Kit (Thermo Fischer Scientific, A38524500) in a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific).Both batches were sequenced in a NovaSeq6000 sequencer (Illumina ...
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bioRxiv - Developmental Biology 2022Quote: ... Gene expression was measured using real time qPCR with PowerUp SYBR Green Master mix on a QuantStudio 6 instrument (Thermo Fisher Scientific, Waltham, MA). Results were normalized to RPS9 (ribosomal protein S9 ...
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bioRxiv - Microbiology 2019Quote: ... A total of 6 μg of total RNA was reverse-transcribed with random primers using the High-Capacity cDNA Reverse Transcription Kit (Applied Biosystems, Foster City, CA). qRT-PCR was conducted using the Applied Biosystems One-Step Real-time PCR protocol using SYBR Green fluorescence (Applied Biosystems ...