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Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2020Quote: ... Peptides were then resuspended in 50 μL of 50 mM HEPES buffer and isobarically labeled using 400 μg of Tandem Mass Tag 6-plex (TMT6) isobaric labeling reagent (Thermo Fisher Scientific). The labeling reactions were then quenched with 4 μL of 5% hydroxylamine ...
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bioRxiv - Neuroscience 2020Quote: ... 500 000 cells per well were seeded in a 6-well-plate in Dulbecco’s Modified Eagle Medium (DMEM) (Gibco/Thermo Fisher, France) with 10% Fetal Bovine Serum (FBS ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... plasma and urinary mtDNA in samples was quantified by the following thermal profile used with QuantStudio™ 6 Flex System (Applied Biosystems, Carlsbad ...
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bioRxiv - Immunology 2021Quote: ... Supernatants were collected 6 days post transfection by centrifugation at 4,000g followed by filtration through a 0.22 μM filter (Thermo Fisher Scientific, Cat#: SE1M179M6). Clarified supernatants were then incubated with Protein A agarose beads (Thermofisher ...
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bioRxiv - Microbiology 2021Quote: ... 5 x 105 HCT116 cells were seeded in a 6 well plate with Dulbecco’s modified Eagle medium (DMEM, Gibco®, Life Technologies) supplemented with 10% fetal bovine serum (FBS ...
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bioRxiv - Immunology 2020Quote: ... FP8-GGGC peptides were conjugated to Qβ-VLPs using the bifunctional cross-linker succinimidyl 6-[(β-maleimidopropionamido) hexanoate] (SMPH; Thermo Fisher Scientific) as described previously for the conjugation of amyloid-beta (Aβ ...
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bioRxiv - Biochemistry 2019Quote: ... The protein concentration was determined after denaturing the proteins with 6 M guanidine hydrochloride by measuring absorbance at 280 nm and 496 nm with a NanoDrop Spectrophotometer (Thermo Fisher Scientific). The concentration was calculated using the following equation:
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bioRxiv - Immunology 2021Quote: ... On day 10-11 EB were harvested by gentle pipetting and 30 EB/well were plated on gelatin-coated 6-well plates in RPMI (Thermo Fisher Scientific), 10% FCS (PAN Biotech ...
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bioRxiv - Evolutionary Biology 2020Quote: ... Following electroporation the cells were seeded across 3-wells of a 6 well plate coated with Matrigel in StemFlex supplemented with RevitaCell supplement (Thermo Fisher, A2644501). After 3 days from electroporation the cells were selected with StemFlex supplemented with G418 (25 µg/ml ...
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bioRxiv - Cell Biology 2019Quote: ... Cells were pelleted by centrifugation at 10628 g at 25°C for 10 min (Thermo Fisher Scientific, #F9-6×1000 LEX rotor) and washed once with sterilized water prior to re-suspension into (MM ...
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bioRxiv - Neuroscience 2021Quote: ... with transfection reagents containing the siRNAs (final concentration: 6 nM in culture medium) and Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific; #13778030) in Opti-MEMI following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... siRNA and the negative control siRNA (siNC) with 20 nM in 6-well plates were transfected using Liopfectamine 3000 (Invitrogen, Carlsbad, USA) according to the manufacturers’ protocols ...
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bioRxiv - Physiology 2021Quote: ... Quantitative real-time PCR (qPCR) was performed with SYBR Green chemistry using the QuantStudio 6-flex Real-time PCR System (Thermo Fisher Scientific). Expression levels of mouse and human genes were normalized to Actinb or cyclophilin or ACTINB ...
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bioRxiv - Immunology 2020Quote: Wild-type Phl p 6 and its mutants were expressed from pET17b constructs in Escherichia coli strain BL21 Star (DE3; Invitrogen/Thermo Fisher Scientific), as described in the supplement.
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bioRxiv - Developmental Biology 2020Quote: ... Spermatocytes and spermatids isolated from 8-week-old C57BL/6 mice were suspended in αMEM with 10% knockout serum replacement (Thermo Fisher Scientific) and 10 μg/ml GDNF (Funaskoshi) ...
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bioRxiv - Biochemistry 2021Quote: Cells were seeded in 6 well plate format and grown till 70% confluency and the luciferase reporter plasmids were transfected using Lipofectamine 3000 (Thermo Fisher Scientific). One hour prior to harvesting ...
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bioRxiv - Animal Behavior and Cognition 2021Quote: ... sections were rinsed three times for 10 min each and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4′,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific) as counterstaining.
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bioRxiv - Cell Biology 2020Quote: ... Nuclei were counterstained with 2-(4-Amidinophenyl)-1H-indole-6-carboxamidine (DAPI) and appropriate secondary antibodies conjugated to fluorochromes (Thermo Fisher Scientific) were applied for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... and replated at a density of 3*106 cells/well in Matrigel-coated 6-well plates containing NB media supplemented with BDNF (Thermo Fisher PHC7074), GDNF (Thermo Fisher PHC7044) ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were cultured and expanded in 6-well plates coated with Geltrex™ LDEV-Free Reduced Growth Factor Basement Membrane Matrix (A1413202, Gibco Life Technologies) diluted 1:100 in DMEM (31966021 ...
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bioRxiv - Cell Biology 2020Quote: ... cells were typically transfected in a 6 well plate format with 20 μM siRNAs and 4 μL Lipofectamine RNAiMAX (Thermo Fisher Scientific); the depletion phenotype was inspected 72 h after transfection unless otherwise indicated in the text or the legends ...
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bioRxiv - Cell Biology 2020Quote: Glass-bottomed imaging dishes were exposed to UV light for 6 minutes and coated with Poly-D-Lysine (Thermo Fisher Scientific, A3890401) for a minimum of 20 minutes ...
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bioRxiv - Cancer Biology 2021Quote: Tandem mass tag (TMT) labeling was performed for mass spectrometry (MS) using the TMT 6-plex isobaric labeling kit (ThermoFisher, Cat #90111) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... Isolated B cells were stained for 20 minutes on ice with a fluorescence staining-mix containing 4’,6-Diamidin-2-phenylindol (DAPI; Thermo Fisher Scientific), anti-human CD20-Alexa Fluor 700 (BD) ...
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bioRxiv - Immunology 2021Quote: ... and was conjugated directly to surface lysines on Qβ bacteriophage VLPs using the bidirectional crosslinker succinimidyl 6-[(beta-maleimidopropionamido) hexanoate] (SMPH; Thermo Fisher Scientific) as previously described 10 ...
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bioRxiv - Microbiology 2020Quote: ... Alexa-Fluor 594 labelled goat anti-rabbit IgG, ProLong Gold antifade reagent with DAPI (4’, 6-diamidino-2-phenylindole) (Life technologies-Invitrogen, USA), ATP Affinity kit (no ...
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bioRxiv - Molecular Biology 2020Quote: ... approximately 400.000 cells were plated in a 6-well dish and reverse transfected with a final siRNA concentration of 50 nM using Lipofectamine RNAiMax (Thermo Fisher Scientific). 48 hrs post transfection ...
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bioRxiv - Molecular Biology 2020Quote: ... qPCR was performed using qPCRBIO SyGreen Blue Mix (PCR Biosystems) and run on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems). Primer sequences used were predesigned KiCqStart SYBR® Green primers (Sigma-Aldrich ...
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bioRxiv - Immunology 2020Quote: Primary lymphocyte cell cultures were prepared by passing 6 peripheral nodes (axial, brachial, and inguinal) through a single 70-µm nylon mesh filter (Thermo Fisher, USA) with the rubber tip of the plunger from either a 1-or 3-mL syringe ...
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bioRxiv - Immunology 2020Quote: ... SYBR Green was used as the reporter and the reaction was run for 40 cycles on a QuantStudio 6 PCR instrument (Thermo Fisher, USA). Genes that were detected on or after cycle 35 were considered not expressed ...
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bioRxiv - Immunology 2020Quote: CD3+ T cells were isolated from the spleen of C57BL/6 mice using a negative selection T cell separation kit (#8802-6840-74, Thermo Fisher Science). CD3+ T cells were labeled with the CellTrace carboxyfluorescein diacetate succinimide ester (CFSE ...
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bioRxiv - Immunology 2020Quote: ... Expression of CD69 on the surface of MEL5 TCR+ CD8+ J.RT3-T3.5 cells was measured after 6 h using the following directly conjugated monoclonal antibodies: anti-CD8α–PE-Cy7 (clone RPA-T8; Thermo Fisher Scientific), anti-CD8β–eFluor660 (clone SIDI8BEE ...
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bioRxiv - Physiology 2020Quote: ... and PCR was performed on the QuantStudio 6 Flex system (3 technical replicates per mouse, Applied Biosystems, Iowa Institute of Human Genetics). Gene expression was calculated using the ΔΔct method (39 ...
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bioRxiv - Immunology 2021Quote: ... apoptotic thymocytes were washed twice with PBS and resuspended in PBS at 10^6 cells/mL containing 40 ng/mL pHrodo-SE (Thermo Fisher Scientific) and incubated at RT for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... (Horizon Discovery Dharmacon, Lafayette, CO) in DharmaFECT 3 reagent (Horizon Discovery Dharmacon, T-2003) for 6-8 hours in OptiMEMm (Invitrogen, Waltham, MA)65 ...
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bioRxiv - Cell Biology 2021Quote: ... The expression of mRNA was examined by quantitative PCR analysis using a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems). TaqMan© assays were used to quantitate ERRα (Hs00607062_gH) ...
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bioRxiv - Neuroscience 2021Quote: ... One million pre-differentiated cells were plated to each well of a Nunclon 6-well dish with 2 ml of the high purity differentiation media: DMEM/F12 (Thermo Fisher 21331020) containing high purity N2 (above) ...
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bioRxiv - Cancer Biology 2020Quote: ... The gels were transferred to a nitrocellulose membrane using the iBlot Gel Transfer at P3 for 6 minutes (Thermo Fisher, cat#IB23001) and then blocked for 1 h at room temperature in Odyssey blocking buffer (LICOR Biosciences ...
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bioRxiv - Microbiology 2022Quote: ... trachomatis-infected cells by scraping one or two wells of a 6-well tissue culture plate in a total volume of 500 μL Trizol Reagent (Thermo Fisher Scientific). Samples were transferred to RNase-free o-ring capped tubes containing ∼100 μL volume of zirconia beads and thoroughly vortexed for 10 min to rupture bacterial cells ...
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bioRxiv - Cancer Biology 2022Quote: ... Nuclei were counterstained with DAPI (4’,6-diamidino-2-phenylindole) and filamentous actin with Alexa Fluor 488-conjugated phalloidin (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2022Quote: ... The syn-gRNA oligos and syn-gRNA-NTC were transfected using Lipofectamine RNAiMAX transfection reagent (5 µl per 6-well; Thermo Fisher Scientific) following the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2022Quote: ... B16F10-TR-shApoe cells were plated into a 6-well dish the day prior to transfection and transfected with 4 μg plasmid and TurboFect transfection reagent (Thermo Scientific, R0533) diluted in serum-free media ...
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Convection and extracellular matrix binding control interstitial transport of extracellular vesiclesbioRxiv - Bioengineering 2022Quote: ... with a cocktail of functionally inhibitory integrin blocking antibodies (20 μg/mL anti-α3, clone-ASC-6, Millipore Sigma; 20 μg/mL anti-α6, clone-GoH3, Thermo Fisher Scientific ...
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bioRxiv - Biochemistry 2022Quote: ... MDA-MB-231 cells were seeded at a density of 500,000 cells/well in a 6-well plate and transfected with siRNA targeting EED using Lipofectamine RNAiMAX Transfection Reagent (Thermo Fisher Scientific) for 2 days ...
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bioRxiv - Biochemistry 2022Quote: ... The resulting plasmids with Fpn or the empty plasmid were transfected into HEK 293S cells on 6-well plates with 293fectin™ transfection reagent (Invitrogen/Thermo Fisher) per the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2022Quote: ... The coverslips were then washed with 1x PBS and incubated with μg/ml DAPI (4’,6-diamidino-2-phenylindole; Life Technologies, #D1306), followed by mounting as described above.
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bioRxiv - Immunology 2022Quote: ... Bacteria were harvested after 16–18 h at 3000 x g for 15 min at 4°C (Sorvall LYNX 6000 centrifuge, F9-6×1000 LEX rotor, Thermo Fisher Scientific).
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bioRxiv - Immunology 2022Quote: ... Soluble forms of the SOSV antigens with were purified by using the proteins’ 6×-His tag or StrepII tags and Expi293F cells and Expifectamine 293 expression system (Thermo Fisher Scientific) as discussed below at day 5 to 7 post-transfection ...
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bioRxiv - Immunology 2022Quote: ... Cells were then washed with DPBS before being stained with 50 μL/well of DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride) (Invitrogen, cat. #D1306) diluted to 5 μM in DPBS for 15 min ...
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bioRxiv - Plant Biology 2022Quote: ... The RT-qPCR was performed using the ChamQ Universal qPCR Master Mix (Vazyme Biotech) on the QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems). Relative transcript abundances of the selected genes were calculated with the ddCt method (52 ...