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Citations for Thermo Fisher :
4651 - 4700 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Physiology 2024Quote: ... control siRNA or siRNA targeting ADAR1 (30 pmol per well in 6-well plate) and Lipofectamine RNAiMAX transfection reagent (9 µL; Cat. # 13778075; Thermo Fisher Scientific, Dreieich, Germany) were used to transfect the cells according to manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... A total volume of 10µl per reaction was run on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems Thermo Fisher Scientific, 4485691) for 45 cycles (2 minutes at 50 °C ...
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bioRxiv - Genomics 2024Quote: ... HAECs at low passage (passage 3-6) were treated prior to harvest every 2 days for 7 days with either 10 ng/mL TGFB2 (ThermoFisher Scientific, cat. no. 302B2002CF), IL1B (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2024Quote: ... 100 ng of cDNAs were amplified in 1X TaqMan™ Fast Advanced Master Mix and 1X TaqMan™ Assay in a 20-µL reaction using the QuantStudio 6 Flex real-time PCR Platform (Thermo Fisher Scientific Inc.) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2024Quote: ... overnight incubation in a cold room (6-7°C) was carried and anti-mouse/rabbit secondary antibody Alexa-488 conjugated (Thermo Fisher Scientific, Waltham, MA) incubation was performed (1 hour ...
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bioRxiv - Neuroscience 2024Quote: ... Sections were stained with DAPI (4’,6-diamidino-2-phenylindole) and cover-slipped with ProLong Glass Antifade Mountant (P36984, Thermo Scientific, Waltham MA, USA). All images were acquired on a Keyence-BZ800 microscope ...
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bioRxiv - Immunology 2021Quote: ... supplemented with 1% FBS and 1 μM Indo-1 AM (Invitrogen), and incubated at 37°C for 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... α-zona occludens-1 (ZO-1) protein (1:200, Invitrogen, 61-7300) and the lectins Ulex europaeus agglutinin - Atto488 conjugated (UEA ...
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bioRxiv - Cell Biology 2023Quote: ... Whole blood was diluted 1:1 with 1× PBS (Thermo Fisher) and layered over Lymphoprep followed by density gradient centrifugation ...
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bioRxiv - Synthetic Biology 2024Quote: ... incubated with VCAM-1 monoclonal antibody (1:100, 1.G11B1 Invitrogen) at 4°C overnight ...
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bioRxiv - Cancer Biology 2024Quote: ... Cycle 1 GR-1 (Invitrogen, 14-5931-82, 1:100, 1hr), Cycle 2 F4/80 (Cell Signaling ...
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bioRxiv - Biophysics 2021Quote: ... ∼1 nM YOYO-1 (ThermoFisher, #Y3601), 1000 units of catalase (Millipore Sigma ...
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bioRxiv - Cell Biology 2020Quote: ... anti-COX-1 (Invitrogen, 1:100) and anti-COX-2 (Santa Cruz ...
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bioRxiv - Developmental Biology 2020Quote: ... BrdU (1:500, Invitrogen MoBU-1), Ki67 (1:2000 ...
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bioRxiv - Immunology 2019Quote: ... 1% Primocin (Invitrogen; ant-pm-1) for 5 minutes and kept on ice in a sterile container with PBS soaked gauze until transplantation ...
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bioRxiv - Developmental Biology 2020Quote: ... ZO-1 (1:200, ThermoFisher Scientific) YAP (Cell 1:200 ...
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bioRxiv - Developmental Biology 2022Quote: ... 1% 1%ITS-X (Gibco, 51500056), 8 nM β-estradiol (Sigma ...
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bioRxiv - Neuroscience 2021Quote: ... and accutase (1:1) (Gibco, A11105) for 8 min at 37°C and later blocked using 0.5 mg/ml trypsin inhibitor (Gibco ...
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bioRxiv - Biophysics 2022Quote: ... 1% GlutaMAX-1 (35050–001, Gibco), 1% nonessential amino acids (11140–050 ...
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bioRxiv - Neuroscience 2021Quote: ... and 1% GlutaMAX-1(Gibco, 35050061) was added to stop the digestion ...
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bioRxiv - Developmental Biology 2022Quote: ... 1:1 ProLong Gold (Invitrogen, USA).
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bioRxiv - Developmental Biology 2022Quote: ... anti-ZO-1 @ 1:500 (Invitrogen, cat#40-220D* ...
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bioRxiv - Neuroscience 2024Quote: ... Iba-1 (1:200, GT10312, Invitrogen), CD68 (1:400 ...
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bioRxiv - Neuroscience 2024Quote: ... 1×GlutaMAX-1 (Gibco, 35050-061), 1×β-Mercaptoethanol (Invitrogen ...
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bioRxiv - Developmental Biology 2024Quote: ... and 1:1 Eosin (Fisher Scientific): 80% ethanol ...
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bioRxiv - Neuroscience 2023Quote: ... 1:1 000 (Thermo Fisher Scientific) coupled to the blue ...
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bioRxiv - Microbiology 2021Quote: ... cDNA was diluted 1:10 for quantitative real-time PCR (qPCR) run on an Applied Biosystems QuantStudio™ 6 fast real-time PCR system (Applied Biosystems, Foster City, CA) using PowerUp SYBR green master mix (Life Technologies) ...
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bioRxiv - Cell Biology 2022Quote: ... Cells nuclei were counter-stained using 4′,6-Diamidine-2′-phenylindole dihydrochloride (DAPI) mounting medium (ProLong™ Diamond Antifade Mountant with DAPI, Thermo Fisher Scientific, Warsaw, Poland). Preparations were observed using the confocal microscope under magnification 630× and processed with Fiji software (ImageJ 1.52n ...
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bioRxiv - Neuroscience 2021Quote: ... sections were rinsed and mounted immediately after onto glass slides coated with gelatin in Fluoromont-G with 4’,6-diamidino-2-phenylindole (DAPI) (00-4959-52, Invitrogen, Thermo Fisher Scientific, MA, USA) as counterstaining.
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bioRxiv - Immunology 2020Quote: ... were stained with 0.25 ug of Mamu MR1 5-OP-RU or Ac-6-FP tetramer for one hour in the presence of 500 nM Dasatinib (Thermo Fisher Scientific; Cat No. NC0897653). When TCRVα7.2 co-staining was performed ...
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bioRxiv - Cancer Biology 2019Quote: ... Individual samples were then labeled with isobaric tags using commercially available Tandem Mass Tag™ 6-plex (TMTsixplex™) (Thermo Fisher Scientific, P/N 90061) or TMT11plex (TMT11plex™ ...
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bioRxiv - Microbiology 2020Quote: ... qRT-PCR was performed using TransStart Top Green qPCR SuperMix (TransGen Biotech) on a QuantStudio TM 6 Flex Real-Time PCR System (Applied Biosystems, Foster City, CA, USA) with the following thermal cycling parameters ...
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bioRxiv - Molecular Biology 2020Quote: ... Real-time qPCR reactions were carried on using power up SYBR green master mix on a QuantStudio™ 6 Flex Real-Time PCR System (Applied Biosystems, Life Technologies Inc.). Ct (cycle threshold ...
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bioRxiv - Cancer Biology 2020Quote: Gene-specific knockdown was achieved by reverse-transfection of PCa cell suspensions (total 5×105 cells) with 12.5 nM siRNA in 6 well plates using RNAiMAX transfection reagent (Life Technologies; Thermo Fisher Scientific, Scornsby, VIC, AUS), according to the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2022Quote: A concentration of 6 × 103 cells was loaded with 5 mM 4-amino-5-methylamino-2’,7’- difluorofluorescein diacetate (DAF-FM, Molecular Probes, Thermo Fisher, Sao Paulo, Brazil) after 72 h of treatment ...
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bioRxiv - Cell Biology 2022Quote: ... collected every 8 hours from 24 to 72 hours post-stimulation on day 6 were lysed in radioimmunoprecipitation (RIPA) lysis buffer (Pierce, Thermo Fisher Scientific; Cat. No.: 89900) containing protease inhibitors (Sigma-Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... per well were distributed in Ibidi® µ-slide 8-well chambered coverslips and stained with 0.8 μM of the membrane specific fluorescent styryl dye N-(3-triethylammoniumpropyl)-4-(6-(4-(diethylamino) phenyl) hexatrienyl) pyridinium dibromide (FM4-64; Thermo Fisher Scientific, Waltham, MA, USA) in the presence of 0 μM (control) ...
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bioRxiv - Evolutionary Biology 2022Quote: ... Several donor colonies and one recipient colony were independently inoculated in 2 mL of LB in 15-mL tubes and cultured for 6 hours (37°C and 250 rpm, Thermo Scientific™ MaxQ™ 8000). Cultures were centrifuged (15 minutes ...
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bioRxiv - Cancer Biology 2019Quote: ... was cultured in RPMI with 5 % heat inactivated human serum (HS) and 2 ng/mL interleukin (IL)-6 (Gibco, Thermo Fisher Scientific, Waltham, MA, USA). In vitro experiments with KJON cells were performed with 2 % HS in RPMI and IL-6 (1 ng/mL) ...
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bioRxiv - Genetics 2021Quote: Total RNA was extracted from issues of C57BL/6 mice and isolated distal convoluted tubule (DCT) cells of parvalbumin-GFP mice using Trizol Reagent (Thermo Fisher Scientific, Waltham, MA, USA) according to manufacturer’s protocol ...
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bioRxiv - Genetics 2019Quote: ... qPCR assays were performed using the Kapa SYBR Fast Universal 2X qPCR Master Mix (Geneworks, Thebarton, South Australia, Australia) on the QuantStudio 6 Flex (Applied Biosystems, Foster City, CA, USA) following these steps ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The polymer solution was added dropwise to 16 mL citric acid solution 250 mM at pH 2.0 at 300 mOsmol/kg under sonication in an ice bath using the following parameters: initial amplitude 50 (6 mm sonotrode, Fisher Scientific Model 705 Sonic Dismembrator) during the addition of the oil phase (approx ...
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bioRxiv - Biochemistry 2021Quote: ... Enzyme assays were conducted in 0.5 mL containing 50 mM 2-(N-morpholino)ethanesulfonic acid (pH 6) and various concentrations of soluble starch (Acros Organics, No. AC424495000, Morris Plains, NJ). The assays also included 100 mM KCl ...
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bioRxiv - Microbiology 2021Quote: ... were stained with 0.25 ug of Mamu MR1 5-OP-RU or Ac-6-FP tetramer for one hour in the presence of 500 nM Dasatinib (Thermo Fisher Scientific; Cat No. NC0897653). When TCRVα7.2 co-staining was performed ...
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bioRxiv - Immunology 2021Quote: ... with an N-terminal IL-2 signal peptide for secretion and a C-terminal 6×His tag for purification was inserted into pFUSE-vector (Invitrogen, Thermo Fischer Scientific, MA, USA). The human ACE2 was expressed by essentially the same protocol used for the COVID-19 RBD ...
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bioRxiv - Plant Biology 2019Quote: ... pollinated stigma was incubated in FM™ 4-64 Dye (N-3-Triethylammoniumpropyl-4-6-4-Diethylamino Phenyl Hexatrienyl Pyridinium Dibromide, Life Technologies T3166, 8.23 μM) for five minutes and subsequently washed in 1/2 Murashige and Skoog basal medium containing 10 % (w/v ...
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bioRxiv - Genomics 2021Quote: ... The embryoid medium was aspirated and 15 to 20 embryoid bodies were plated per well in 6-well plates and cultured in 3 mL hematopoetic medium (2 mM GlutaMax, 1x Antibiotic-Antimycotic (Thermo Fisher Scientific; Cat. No. 15240062), 55 mM 2-mercaptoethanol (BioRad ...
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bioRxiv - Cancer Biology 2022Quote: ... To quantitate cell migration 6 fields of view per membrane were photographed with EVOS™ FL Auto 2 Imaging System(Thermo Scientific™ Invitrogen™), followed by quantification by ImageJ v1.53q (National Institutes of Health ...
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bioRxiv - Neuroscience 2022Quote: ... A total of 6 samples were prepared for proteomics analyses by means EasyPepTM Mini MS Sample Prep Kit (Thermo Fisher Scientific, Rock ford, IL, USA) for proteins extraction ...
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bioRxiv - Neuroscience 2024Quote: ... then separated on a reverse-phase nano-spray column (2 μm, 75 mm i.d. × 50 cm, 100 Å, Thermo Fisher Scientific Cat# 16-494-6) using gradient elution ...