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Citations for Thermo Fisher :
4351 - 4400 of 10000+ citations for SpectraDye Antibody Labeling Kit IR800 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and resuspended in an antibody cocktail containing fluorophore-conjugated antibodies (Supp. Table 2) and LIVE/DEAD Fixable Blue stain (ThermoFisher, #L34962) in BD Horizon Brilliant Stain Buffer (BD Biosciences ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed for 3X in PBS followed by secondary antibody incubation (all secondary antibodies were incubated in 1:200 dilutions: anti-mouse Fisher Scientific Cat no ...
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bioRxiv - Biochemistry 2022Quote: ... secondary antibody HRP conjugated (31460) and goat anti-mouse IgG (H+L) secondary antibody HRP conjugated (31430) were from Thermo Scientific and rabbit anti-ATP13A3 antibody (HPA029471 ...
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bioRxiv - Cancer Biology 2023Quote: ... incubated overnight with primary antibodies at 4 degrees and detected with goat anti-rabbit or mouse-HRP secondary antibodies (Thermo Scientific) followed by detection using SuperSignal™ West Pico Plus chemiluminescent substrate (Thermo Scientific).
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bioRxiv - Developmental Biology 2023Quote: ... Sections were incubated with primary antibody overnight at 4°C and then incubated with Alexa Fluor-conjugated donkey secondary antibodies (1:400, Molecular Probes) at RT for 2 hrs and then mounted in VECTASHIELD medium with 4′-6-Diamidino-2-phenylindole (DAPI ...
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bioRxiv - Cancer Biology 2023Quote: ... they were incubated in secondary antibody conjugated to different fluorophores (Alexa 555-labeled anti-rabbit and Alexa 488-labeled anti-mouse secondary antibody (Thermo Fisher)) and DAPI for 1 hour at room temperature ...
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bioRxiv - Systems Biology 2023Quote: ... The following primary antibodies were used: 10 ul of allophycocyanin (APC)-labeled anti-CD2 antibody (clone LT2; Invitrogen, Catalog # MA1-10132), 5 uL of APC-labeled anti-CD81 antibody (clone 1D6 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then incubated overnight with rabbit anti-p-ERM primary antibody (1:5000, Roubinet, 2011) followed by goat anti-rabbit Alexa Fluor 488-conjugated secondary antibody (1:200, Invitrogen #A11070) for 1h ...
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bioRxiv - Developmental Biology 2023Quote: ... Samples were incubated in a solution of secondary antibodies diluted in blocking buffer (antibodies and dilutions described in Table 3) and DAPI (1 μg/mL; ThermoFisher Scientific) for 30 minutes at RT in the dark ...
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bioRxiv - Cell Biology 2023Quote: ... samples were incubated for 2h at room temperature (in the dark) with fluorescently labelled secondary antibodies (Invitrogen Alexa Fluor Plus antibodies) diluted (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... were further incubated with fluorophore-conjugated secondary antibodies (goat anti-Mouse IgG2a Cross-Adsorbed Secondary Antibody, Alexa Fluor 594, Thermo Scientific, cat# A-21135 ...
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bioRxiv - Neuroscience 2023Quote: ... were selected and processed as described above with primary antibody Rabbit anti-Cox-2 (1:1k, Cayman Chem, 160123) and secondary antibody AF555 donkey anti-rabbit (1:200, ThermoFisher, A31572).
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bioRxiv - Biochemistry 2023Quote: ... Horseradish peroxidase-conjugated secondary antibodies against rabbit (catalogue number: 31460) and mouse IgG (catalogue number: 31430) were Pierce antibodies from Thermo Scientific. Adenosine-5’-Triphosphate (ATP) ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was then incubated with a secondary antibody: goat anti-rabbit IgG (H+L) antibody (1:5000; Thermo Fisher Scientific) or Goat anti-mouse IgG (H+L ...
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bioRxiv - Bioengineering 2023Quote: ... the samples were left to incubated at room temperature for 90 minutes with the corresponding secondary antibodies (diluted 1:200 in PBS): Alexa Fluor® 488 Goat Anti-Rabbit IgG Antibody (Product #A-11008, Invitrogen) and Anti-Mouse IgG Secondary Antibody ...
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bioRxiv - Developmental Biology 2023Quote: ... Bound primary antibodies were detected with HRP-conjugated secondary antibody and chemiluminescence assay (SuperSignalTM West Pico PLUS Chemiluminescent Substrate, Thermo Scientific). Total and di-phospho MPK-1B and alpha-tubulin levels were quantified by measuring the corresponding band intensities using built-in measurement tools of Fiji [70] ...
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bioRxiv - Developmental Biology 2023Quote: ... Bound primary antibodies were detected with HRP-conjugated secondary antibodies followed by a chemiluminescence assay (SuperSignalTM West Dura Extended Duration Substrate, Thermo Scientific). To inactivate HRP-conjugates and proceed with the incubation of the primary anti-alpha tubulin antibody ...
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bioRxiv - Microbiology 2023Quote: ... Blocking was performed for 1h (5% skimmed milk in Tris-buffered saline containing 0.5% Tween) and primary antibody staining was carried out overnight at 4°C (polyclonal anti-PBP2a, custom antibody from Thermo Fisher, 1:1000 or polyclonal anti-Sortase A ...
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bioRxiv - Microbiology 2023Quote: ... were added as the primary antibodies and HRP-labelled goat anti-human IgE antibody (at 1:1,000 dilution, Invitrogen, Waltham, MA) was used as the secondary antibodies ...
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bioRxiv - Genetics 2023Quote: ... Cells were incubated with primary antibodies overnight at 4 °C and probed with the appropriate secondary antibodies for 1 hour at room temperature (ThermoFisher Scientific). Primary and secondary antibodies were resuspended in 0.2% Gelatin in PBS ...
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bioRxiv - Immunology 2023Quote: ... transferred to nitrocellulose membranes and the membranes were probed with the following antibodies: a rabbit anti-MAVS polyclonal antibody (Thermo Fisher), a rabbit anti-STING polyclonal antibody (Cell Signaling) ...
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bioRxiv - Molecular Biology 2023Quote: ... Incorporation of CldU was detected using rat-anti-BrdU antibodies (1:500; BU1/75, AbD Serotec) and Alexa fluor-555-labelled goat-anti-rat antibodies (1:500; Molecular Probes), whereas incorporated IdU was detected using mouse-anti-BrdU antibodies (1:750 ...
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bioRxiv - Cancer Biology 2023Quote: ... TRAIL blocking ab was purchased from Santa Cruz TRAIL Antibody (RIK-2): sc-56246, and IFN gamma blocking Monoclonal Antibody (NIB42, #6-7318-81) was purchased from Thermo Fisher. The blocking mAb was added when NK cells were combined with tumor cells.
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bioRxiv - Cell Biology 2023Quote: ... Membranes were washed and then incubated with Horseradish peroxidase secondary antibodies against the last antibody: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen ® ...
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bioRxiv - Cell Biology 2023Quote: ... followed by PBS-Tween washes and secondary antibody incubation (2h at room temperature): Horseradish peroxidase secondary antibodies: mouse (Gt anti-mouse IgG -H+L-Invitrogen®) 1:10000 and rabbit (Gt anti-Rb IgG -H+L-Invitrogen® ...
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bioRxiv - Neuroscience 2023Quote: ... The vasculature was incubated overnight in primary antibodies (all primary antibodies were incubated in 1:200 dilutions: SK Cat no. AB2200865, IK Fisher Scientific Cat no ...
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bioRxiv - Neuroscience 2023Quote: ... membranes were washed and then incubated with the corresponding secondary antibodies for 1h at room temperature (Goat Anti-rabbit Secondary Antibody, HRP conjugate 1:1000, ThermoFisher #31460). All antibodies were diluted in a 5% BSA solution in TBS-T ...
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bioRxiv - Neuroscience 2024Quote: ... Secondary antibodies were as follows: isotype-specific Alexa Fluor 488- and Alexa Fluor 546- conjugated goat anti-rat IgG antibodies (Thermo Fisher Scientific Cat# A-21434 ...
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bioRxiv - Microbiology 2024Quote: ... Rabbit anti-BAG1 primary antibodies (1:1000; Carruthers Lab) and goat anti-rabbit Alexa Fluor 594 secondary antibodies (1:1000; Invitrogen; #A11012) were utilized ...
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bioRxiv - Cancer Biology 2024Quote: ... Appropriate primary antibodies and HRP-conjugated secondary antibodies were used and proteins were detected using the Enhanced Chemiluminescent (ECL) reagent (Thermo Scientific). The images were acquired with ChemiDoc MP System (Bio-Rad) ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:500 anti-Mouse IgG-HRP-linked whole ab sheep antibody (Cytiva NA931V) and 1:500 anti-rabbit IgG-Alexa 488-conjugated donkey antibody (Invitrogen A21206), (2 ...
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bioRxiv - Cancer Biology 2024Quote: ... 1:500 anti-Mouse IgG-HRP-linked whole ab sheep antibody (Cytiva NA931V) and 1:500 anti-rabbit IgG-Alexa 647-conjugated donkey antibody (Invitrogen A31573). Washed three times for 30 minutes each in TNTT at 4 ℃ to remove secondary antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... The following secondary antibodies were used: goat anti-mouse IgG (H + L) highly cross-adsorbed secondary antibody Alexa Fluor 488 (Invitrogen/ThermoFisher), and goat anti-rabbit IgG (H + L ...
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bioRxiv - Cell Biology 2024Quote: ... Afterwards cells were labelled with fluorescence primary antibodies (Table 2) followed by Alexa FluorTM 488 and 568 labelled secondary antibodies and Phalloidin Alexa FluorTM 647 (Invitrogen, A22287) staining ...
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bioRxiv - Neuroscience 2024Quote: ... the cells were incubated overnight at 4°C with an anti-HIV-1 p24 monoclonal (AG3.0) primary antibody (4 µl/mL) (NIH HIV Reagent Program catalog# 4121) or P2RY12 primary antibody (1:100, 702516, Thermo Fisher) or TMEM119 primary antibody (1:50 ...
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bioRxiv - Immunology 2024Quote: ... Chemiluminescence was used to detect the secondary antibody (Anti-rabbit IgG, HRP-linked Antibody #7074 and SuperSignal™ West Pico PLUS Chemiluminescent Substrate, ThermoFisher).
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bioRxiv - Developmental Biology 2020Quote: ... using the mMessage mMachine T7 or SP6 kit and Poly(A) Tailing kit (Ambion), purified with RNeasy purification kit (Qiagen) ...
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bioRxiv - Developmental Biology 2020Quote: ... then purified with MEGA clear kit (all the kits were from Thermo Fisher Scientific). CB6F1 (C57BL/6 × BALB/c ...
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bioRxiv - Cell Biology 2019Quote: ... cDNA was synthesized using a commercial kit (SuperScript III First-strand synthesis kit, Invitrogen) using random hexamers according to kit instructions ...
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bioRxiv - Cell Biology 2021Quote: ... using the mMessage mMachine T7 or SP6 kit and Poly(A) Tailing kit (Ambion), purified with RNeasy purification kit (Qiagen) ...
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bioRxiv - Neuroscience 2020Quote: ... using the mMESSAGE mMACHINE T7 ULTRA kit and the MEGAshortscript T7 kit (Life Technologies), respectively ...
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bioRxiv - Biochemistry 2020Quote: ... the GeneJET Plasmid Miniprep Kit and the GeneJET Gel Extraction Kit from Thermo Scientific were used ...
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bioRxiv - Biophysics 2020Quote: ... Guide RNAs were expressed and purified using commercial kits (MEGAscript T7 Transcription Kit, Invitrogen and mirVana miRNA isolation Kit ...
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bioRxiv - Biophysics 2019Quote: ... cRNA was transcribed using in vitro transcription kits (T7 RNA expression kit; Ambion Invitrogen). After 12-24 hours from harvesting and defoliculation ...
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bioRxiv - Biophysics 2019Quote: ... cRNA was transcribed using in vitro transcription kits (T7 RNA expression kit; Ambion Invitrogen). After 12-24 hours from harvesting and defoliculation ...
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bioRxiv - Biochemistry 2019Quote: ... polyadenylated with a Poly(A) Tailing Kit and purified with a MEGAclear Kit (Ambion).
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bioRxiv - Molecular Biology 2022Quote: ... The kit used was the SuperScript III Platinum One-Step qRT-PCR kit (Invitrogen) according to the protocol described in (Sibai ...
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bioRxiv - Cell Biology 2024Quote: ... kit and cDNA was synthesized using the SuperScript™ III Reverse Transcriptase kit (Invitrogen, ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... with Neon transfection kit (Invitrogen) using 2 1350V ...
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bioRxiv - Cell Biology 2020Quote: ... with Neon transfection kit (Invitrogen) using 2 1350V ...