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Citations for Thermo Fisher :
4501 - 4550 of 10000+ citations for SpectraDye Antibody Labeling Kit IR800 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biophysics 2021Quote: ... Cultured cells were stained with mAb 0614 or human chimera MAb 0614-5 as the primary antibody and Alexa Fluor 488– or 647–conjugated anti-rat or human-Ig polyclonal antibody (Thermo Fisher Scientific) as the secondary antibody ...
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bioRxiv - Cancer Biology 2021Quote: ... Triton X-100 and 40 mg/mL BSA for 30 min and incubated with primary antibodies for 3 h at room temperature and secondary antibodies in accordance with the manufacturer’s protocol (Life Technologies/Thermo Fisher Scientific). The samples were viewed and analyzed under an LSM880 laser scanning microscope using a 20x air objective (Carl Zeiss) ...
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bioRxiv - Cancer Biology 2021Quote: ... They were then incubated with primary antibodies for 3 h at room temperature and with secondary antibodies in accordance with the manufacturer’s protocol (Life Technologies/Thermo Fisher Scientific). For cell surface MMP14 staining ...
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bioRxiv - Cell Biology 2019Quote: ... and subjected to immunoprecipitations with goat anti-GFP antibodies (generic goat IgGs as control) or mouse anti-γ-adaptin antibodies (mouse hybridoma C7 antibodies as control) bound to protein G-Sepharose beads (Fisher Scientific). Samples were rotated end-over-end at 4°C for 2 h and washed 2 x in buffer D+ and 1 x in PBS ...
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bioRxiv - Cell Biology 2019Quote: ... Cryosections were allowed to thaw in PBS buffer at room temperature and incubated with primary antibodies followed by Alexa Fluor–conjugated secondary antibodies (Thermo Fisher Scientific), counterstained in DAPI (1;5 ...
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bioRxiv - Cell Biology 2020Quote: ... Fixed cells were then blocked in PBS containing 1% BSA and incubated with primary antibodies and appropriate secondary antibodies (Alexa Fluor; Thermo Fisher Scientific) in PBS containing 0.1% BSA ...
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bioRxiv - Cell Biology 2020Quote: ... The cells were labeled with the rabbit anti-tau (C-terminal) antibody (MilliporeSigma) or the mouse anti-phospho-Tau (Thr181) Clone AT270 antibody (Thermo Fisher Scientific) at room temperature at dilution of 1:1000 in 1% BSA for 1 hour and then labeled with Alexa Fluor488 goat anti-mouse IgG secondary antibody at a dilution of 1:500 for 30 minutes at room temperature ...
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bioRxiv - Genetics 2021Quote: ... incubated with mouse anti-HA antibody and then with the secondary antibody conjugated with Donkey-anti-mouse Alexa 567 (Invitrogen Molecular Probes). DNA was stained with 0.5 mg/ml DAPI (Sigma-Aldrich) ...
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bioRxiv - Cell Biology 2020Quote: ... then incubated with appropriate primary antibodies and HRP or near-IR-conjugated secondary antibodies and visualized using chemiluminescence (SuperSignal West Femto Chemiluminescent Substrate; Thermo Fisher Scientific), or a Li-Cor Odyssey fluorescence scanner ...
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bioRxiv - Microbiology 2019Quote: ... followed by immunostaining with a rabbit polyclonal MUC2 antibody and goat anti-rabbit Alexa-Fluor 488 antibody (Life Technologies, Paisley, United Kingdom). Slides were imaged using a BX51 upright microscope and a Coolsnap EZ camera (OLYMPUS ...
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bioRxiv - Microbiology 2019Quote: ... The SFTSV foci were detected using rabbit anti-SFTS NP antibody (Abclon) and goat anti-rabbit IgG secondary antibody conjugated with alkaline phosphatase (Thermo Fisher Scientific). Viral plaques were visualized by incubation with NBT/BCIP solution (Roche ...
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bioRxiv - Developmental Biology 2020Quote: The primary antibodies used in this study and their dilutions were the following: chicken anti-GFP antibody (Life Technologies, A10262, 1:500), rabbit anti-DsRed (Clontech ...
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bioRxiv - Cell Biology 2022Quote: ... Fixed cells were blocked in 1% (w/v) BSA and incubated in primary antibody and respective secondary antibody (Alexa Fluor; Thermo Fisher Scientific) in 1% (w/v ...
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bioRxiv - Molecular Biology 2021Quote: ... Add 100 µL Pol II antibody-conjugated beads (10 µg Pol II 8WG16 antibody conjugated with Dynabeads™ Protein G for Immunoprecipitation (ThermoFisher, #1004D)) and incubate on a tube rotator for 1 hour in the 4 °C room ...
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bioRxiv - Molecular Biology 2020Quote: ... Secondary antibody incubation was for 1 hour at room temperature using HRP conjugated antibodies: anti-mouse IgG at 1:3000(Invitrogen 62–6520) or goat anti-rabbit IgG at 1:3000 (Promega W4011) ...
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bioRxiv - Molecular Biology 2020Quote: ... Alexa Fluor 488-conjugated goat anti-rat IgG (H + L) antibodies and Alexa Fluor 594-conjugated goat anti-mouse IgG (H + L) antibodies (ThermoFisher Scientific, A11005) were used as secondary antibodies ...
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bioRxiv - Molecular Biology 2020Quote: ... the primary antibody was rinsed three times with PBS then Alexa Fluor 546-tagged goat anti-rabbit IgG secondary antibody (ThermoFisher, A-11035) was added at a 1:1250 dilution and incubated for 1 hour before a final 3x rinse with PBS.
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bioRxiv - Cancer Biology 2019Quote: ... Then coverslips were inverted onto 100 μL of antibody block with secondary antibodies (Alexa Fluor 488 anti-mouse - 1:200, Life Technologies #A11029) and DAPI (DNA ...
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bioRxiv - Immunology 2019Quote: ... Tween 20 and incubated for 2 hr with goat-anti-mouse secondary antibody-Alexa Fluor® 680 conjugate or goat anti-rabbit secondary antibody-Alex Fluor® 800 (0.67 ng/ml; ThermoFisher Scientific). Membranes were washed and developed on the Odyssey Infrared Imaging System (LI-COR) ...
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bioRxiv - Biochemistry 2020Quote: ... washed three times with PBS and added with the secondary antibody (Alexa Fluor 488 Conjugated anti-mouse antibody, Life technologies by ThermoFisher Scientific) at 1:250 for overnight at 4 °C ...
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bioRxiv - Neuroscience 2019Quote: ... and immunostained for 16 h at 4°C with primary antibodies followed by Alexa Fluor® 488- or 546-conjugated secondary antibodies (Molecular Probes) for 1 h at room temperature ...
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bioRxiv - Neuroscience 2019Quote: ... Brains were incubated in primary antibodies (1:25 mouse nc82 and 1:1000 rabbit antibody to GFP A-11120, Thermo Fisher Scientific) at 4 °C for three days ...
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bioRxiv - Cell Biology 2020Quote: GFP was visualized using rabbit polyclonal or mouse monoclonal anti-GFP primary antibodies and Alexa 647-labelled secondary antibodies (Molecular Probes/Invitrogen) directed against rabbit or mouse ...
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bioRxiv - Developmental Biology 2021Quote: ... Primary antibodies (Supplemental Table 1) were incubated at 4°C overnight and secondary antibodies (Alexa Fluo 488, 555, or 647; Thermo Fisher Scientific) were incubated at room temperature for 1 hour ...
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bioRxiv - Genomics 2021Quote: ... The secondary antibody used in this study is Alexa Fluor® 647 Donkey Anti-Rabbit IgG (H+L) Antibody (Life Technologies A31573) used at 1:500 dilution.
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bioRxiv - Neuroscience 2020Quote: ... Visualization of immunoreactive signals of hM4Di was performed using an anti-M4 antibody (H-175) and fluorophore-conjugated secondary antibodies (Invitrogen, Carlsbad, USA) as described previously15.
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bioRxiv - Neuroscience 2020Quote: ... The following secondary antibodies were used here: Alexa Fluor 555-labeled donkey anti rabbit secondary antibody (1:500, Thermo Fisher Scientific, USA), Alexa Fluor 555-labeled donkey anti rat secondary antibody (1:500 ...
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bioRxiv - Bioengineering 2021Quote: ... Washed samples were incubated for 1h at RT with the secondary antibody (1:200 Alexa Fluor 647 goat derived anti-rabbit antibody, Thermo Fisher Scientific). Then ...
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bioRxiv - Bioengineering 2021Quote: ... The antibody probes used were primary rabbit-anti-tGFP antibody (Pierce PA5−22688,) and secondary polyclonal antibody AlexaFluor-647-labeled donkey-anti-rabbit (Invitrogen A-31573). Bovine serum albumin (BSA ...
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bioRxiv - Neuroscience 2021Quote: ... Sections were washed with PBS three times and incubated with secondary antibody (Alexa fluor 488, 546, 633 secondary antibodies, Invitrogen-Molecular Probes) diluted in blocking solution (1:500 ...
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bioRxiv - Neuroscience 2020Quote: ... Brains were incubated with secondary antibody for 3 nights at 4°C with secondary antibodies at 1:500: goat anti-rabbit Alexa Fluor 488 (ThermoFisher #A-11008) and goat anti-mouse Alexa Fluor 647 (ThermoFisher #A-21236) ...
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bioRxiv - Neuroscience 2021Quote: ... Detection of primary antibodies was performed using fluorochrome-conjugated secondary antibodies against goat (Alexa Fluor Plus 647-conjugated, donkey anti-Goat; A32849; Invitrogen; 1:200) and rabbit (Cy3-conjugated ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were incubated in secondary antibodies corresponding to each primary antibody (Alexa Fluor 488 for MAP2 and Alexa Fluor 594 for VGAT, 1:400, Thermo Fisher Scientific) for 40 minutes at room temperature ...
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bioRxiv - Neuroscience 2020Quote: ... The tissue sections were then washed three times in PBS and incubated in secondary antibodies secondary antibodies coupled to Alexa 488 or Alexa 568 (1:300, Molecular Probes, Invitrogen) diluted in the blocking solution for 2 hours at room temperature ...
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bioRxiv - Biochemistry 2021Quote: The following antibodies were used: mouse monoclonal antibody against phosphorylated microtubule-associated protein tau (p-tau, UniProtKB P10637. Thermo Fisher Scientific, MN1020), mouse monoclonal antibody against slow myosin heavy chain I (MYH1 ...
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were blocked in 1% (w/v) BSA and incubated in primary antibody and respective secondary antibody (Alexa Fluor; Thermo Fisher Scientific) in 1% (w/v ...
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bioRxiv - Microbiology 2020Quote: ... Protein bands were detected using a primary antibody followed by an HRP-conjugated secondary antibody (rabbit, Life technologies #G21234, or mouse, Invitrogen #G21040) and visualized via chemiluminescence using Clarity Western ECL Substrate and the ChemiDoc MP Imaging System (BioRad) ...
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bioRxiv - Microbiology 2020Quote: ... were calculated by IFA using primary antibodies against viral proteins and Alexa FluorTM 488 α-rabbit and Alexa FluorTM 586 α-mouse as secondary antibodies (Invitrogen), respectively.
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4 ºC with primary antibodies (for details, see table 2) followed by a 2h incubation in Alexa fluorophore-labeled secondary antibodies (Thermo Fisher Scientific) for immunofluorescence ...
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bioRxiv - Microbiology 2021Quote: ... Cells infected with YFV were incubated with the pan-flavivirus anti-Env 4G2 antibody for 1h at 4°C and then with Alexa 488 anti-mouse IgG secondary antibodies (Thermo Fisher, #A28175) for 45 min at 4°C in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... and incubated with primary antibodies [Anti GFP rabbit polyclonal antibody (1:500 dilution; #catalog no: A-11122, Thermo Fisher Scientific, Tokyo, Japan)] diluted with the blocking solution overnight at 4°C ...
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bioRxiv - Microbiology 2021Quote: ... the parasite mitochondrion was visualized using the Novus anti-HSP60 antibody and AlexaFluor 647-conjugated goat anti-rabbit 2° antibody (Invitrogen Life Technologies A21244), and mACP-HA2 was visualized with the rat anti-HA 3F10 primary antibody and FITC-conjugated donkey anti-rat 2° antibody (Invitrogen Life Technologies A18746) ...
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bioRxiv - Microbiology 2021Quote: ... and mACP-HA2 was visualized with the rat anti-HA 3F10 primary antibody and FITC-conjugated donkey anti-rat 2° antibody (Invitrogen Life Technologies A18746). Images were taken on an EVOS M5000 imaging system or Zeiss 880 laser-scanning confocal microscope fitted with an Airyscan detector ...
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bioRxiv - Molecular Biology 2021Quote: ... the samples were washed with PBS and incubated in the dark for 1 h at 4 °C with secondary antibody (Alexa Fluor-568-conjugated anti-mouse antibody, 1:750, Thermo Fisher Scientific). Finally ...
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bioRxiv - Microbiology 2020Quote: ... sections were incubated with secondary goat IgG Alexa Fluor 488-conjugated anti-rabbit antibody and with goat IgG Alexa Fluor 561-conjugated anti-mouse antibody (Thermo Fisher Scientific) for 1 h at room temperature ...
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bioRxiv - Microbiology 2019Quote: ... fixed in 4% paraformaldehyde and stained with a primary mouse monoclonal antibody raised against IBDV VP2 [18] and a secondary goat anti-mouse antibody conjugated to Alexa Fluor 488 (Thermo Fisher Scientific). Wells were marked positive or negative for the presence or absence of virus by immunofluorescence microscopy and the TCID50/mL calculated by the Reed and Muench method [19] ...
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bioRxiv - Neuroscience 2021Quote: Primary antibody was rabbit anti-GFP primary antibody (1:2000; Chemicon, AB 3080) and secondary antibody was donkey anti-rabbit Alexa Fluor 594 (1:2000; Molecular Probes, A21207). In rats in the GFP group ...
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bioRxiv - Cancer Biology 2020Quote: ... Tissue sections were analyzed by hematoxylin and eosin staining or by immunofluorescence analysis using primary antibodies in conjunction with Alexa Fluor 488/594-conjugated secondary antibodies (Thermo Fisher Scientific) and Hoechst 33342 (Sigma) ...
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bioRxiv - Cell Biology 2021Quote: ... slides were incubated for 45 min at 37°C with a solution containing secondary antibodies (4 μ/ml Alexa Fluor 488– and/or 568–coupled anti-rabbit or anti-mouse antibodies from Molecular Probes) and 1 μg/ml DAPI to visualize DNA in PBST ...
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bioRxiv - Immunology 2021Quote: ... The following day macrophages were then washed with 1X PBS to remove unbound Ki67 antibody and incubated with Alexa Fluor-594 goat anti-rabbit secondary antibody (Invitrogen A-11037) at 1:250 dilution for 2 hrs at room temperature ...