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Citations for Thermo Fisher :
4551 - 4600 of 10000+ citations for SpectraDye Antibody Labeling Kit IR800 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2020Quote: ... monoclonal antibody and further stained with Hoechst (1:10000) and appropriate Alexa Fluor (488/594/647)-conjugated secondary antibodies (Thermo Fisher Scientific). The stained plates were imaged using an automated high-content spinning-disk microscope CQ1 (Confocal Quantitative Image Cytometer ...
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bioRxiv - Biophysics 2021Quote: ... After washing thrice with PBS-0.1% Tween 20 the membrane was probed with primary antibody which is a rabbit monoclonal anti human lamin A+C antibody (Thermo Scientific, USA) and Stabilized Peroxidase Conjugated secondary Goat Anti-Rabbit (H+L ...
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bioRxiv - Cancer Biology 2021Quote: ... Primary antibodies were incubated overnight at 4°C followed by washing and incubation with alexa-conjugated secondary antibodies (Invitrogen, 1:1000 dilution) at room temperature for 1-3 hour in the dark ...
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bioRxiv - Bioengineering 2022Quote: ... cluster of differentiation 31 (CD31, rat IgG2a) primary antibody and alpha-smooth muscle actin (α-SMA, rabbit IgG) primary antibody were purchased from Thermo Fisher Scientific (Waltham ...
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bioRxiv - Neuroscience 2022Quote: ... The primary antibody incubation was followed by 2 h incubation at room temperature with corresponding fluorescent secondary antibodies (1:500, Thermo Fisher Scientific). Nuclei were counterstained with DAPI (1:2000 in 0.1 M PB ...
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bioRxiv - Cancer Biology 2022Quote: ... p53BP1 primary antibodies (Cell Signalling Technologies) were used for foci immunostaining alongside the Alexa Fluor® 488-conjugated secondary antibody (Fisher Scientific).
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed to remove the excess of primary antibody and incubated with the appropriate Alexa-Fluor secondary antibodies for 2 h at 25 °C (1:1000, Thermo Fisher Scientific). The excess of secondary antibody was washed ...
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bioRxiv - Developmental Biology 2020Quote: ... 5um serial sections were generated and used for immunofluorescence with primary antibodies recognizing sarcomeric myosin (1:20, DSHB MF20) and Alexa Fluor-conjugated secondary antibodies (1:1000, Thermo Fisher Scientific). Antigen retrieval was performed on all sections with 10mM sodium citrate buffer ...
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bioRxiv - Neuroscience 2020Quote: ... Secondary antibody solution contained 2 secondary antibodies in blocking solution: Goat anti-mouse Alexa-Flour (AF) 568 (Invitrogen A-11004, 1:400), Goat anti-chicken AF-488 (Invitrogen A11039 ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatant was kept after spinning at 800 rcf for 2’ at +4°C and were incubated overnight at +4°C with 3µg of dedicated antibodies: Nr2f1 antibody (Thermo Fisher PA5-21611) and GFP antibody (Abcam ab13970 ...
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bioRxiv - Developmental Biology 2021Quote: ... as the primary antibodies and then stained with anti-mouse-Alexa488 and anti-rabbit-Alexa594 as the secondary antibodies (Thermo Fisher Scientific) respectively and co-stained with DAPI (Sigma) ...
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bioRxiv - Developmental Biology 2020Quote: ... samples were incubated in fluorophore conjugated Goat anti-Rabbit antibody or Goat anti-Mouse antibody (in 1:400 dilution; Thermo Fisher Scientific) for 2 hours at RT with blocking solution as diluent ...
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bioRxiv - Neuroscience 2021Quote: ... and immunostained with mouse monoclonal anti-SARM1 primary antibody (Chen et al., 2011) followed by Alexa Fluor 488 or 647 anti-mouse secondary antibody (Thermo Fisher Scientific). Fluorescence microscopy images were acquired on a DMi8 upright fluorescence microscope (Leica microsystems ...
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bioRxiv - Microbiology 2021Quote: ... cells were fixed with 4% PFA for 30 minutes and stained for intracellular ENV with 1A1D-2 (1/500) monoclonal antibody and anti-mouse 647-conjugated antibody (2 μg/ml, Invitrogen Cat# A21235).
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bioRxiv - Neuroscience 2020Quote: ... 15 min each and then incubated the brain sections in 2° antibodies (1:700 for Dylight-/Alexa-conjugated antibodies made in donkey purchased from Thermo Fisher Scientific) in 5% BSA/PBST for 2 hrs at RT ...
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bioRxiv - Neuroscience 2020Quote: ... The cells were then treated for 60-90 min with a secondary antibody solution containing goat anti-guinea-pig IgG (H+L) antibody conjugated with Alexa Fluor 594 (A-11076; Thermo Fisher Scientific) (1,000× dilution) ...
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bioRxiv - Systems Biology 2021Quote: ... the cells were washed three times with 100 μL of PBS manually and stained with diluted secondary antibody solution (Alexa-Fluor 568-conjugated goat anti-rabbit IgG antibody) with DAPI (ThermoFisher #A-11011). After incubating for 1 h at room temperature ...
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bioRxiv - Developmental Biology 2022Quote: ... The rat anti-Sls antibody (anti-Kettin, MAC155/Klg16, Babraham Bioscience Technologies) was diluted 1:1000 and visualised with fluorescently labelled secondary antibodies (ThermoFisher, Molecular Probes). Actin was stained with phalloidin-rhodamine or phalloidin-Alexa488 (1:2000 ...
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bioRxiv - Developmental Biology 2022Quote: ... followed by overnight incubation with primary antibody overnight at 4 °C (1:100 rabbit-anti-HNF4A Cell signaling 3113S; 1:250 mouse anti-AFP antibody, Sigma A8452; 1:100 mouse anti CYP3A4 antibody, Life Technologies MA5-17064 ...
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bioRxiv - Microbiology 2022Quote: ... Bound primary antibody was then labeled for 1h at room temperature using Alexa488 goat anti-mouse IgG secondary antibody (Invitrogen, OR, USA) at a 1:750 dilution in PBS ...
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bioRxiv - Zoology 2022Quote: ... while GFP was detected using an anti-GFP rabbit polyclonal primary antibody and an Alexa Fluor 488-conjugated goat anti-rabbit IgG secondary antibody (Thermo Fisher Scientific). Cell nuclei were counterstained with DAPI ...
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Purkinje cardiomyocytes of the ventricular conduction system are highly diploid but not regenerativebioRxiv - Developmental Biology 2022Quote: ... heart tissue sections were incubated at room temperature for 2 hr with secondary antibodies: goat anti-mouse IgG (H+L) cross-adsorbed secondary antibody AlexaFluor 488 conjugate 1:500 (A11001, Thermo Fisher Scientific); goat anti-rabbit IgG (H+L ...
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bioRxiv - Developmental Biology 2022Quote: ... REX1 antibody DA136 (3rd bleed) was diluted at 1:1000 and visualized using Donkey anti-Sheep HRP secondary antibody (Thermo Fisher Scientific). Immunoblots were imaged using enhanced chemiluminescence on a BioRad ChemiDoc imager (BioRad) ...
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bioRxiv - Microbiology 2022Quote: ... The resulting clones were screened via immunoblotting with gene-specific antibodies anti-NINL rabbit polyclonal antibody (Thermo Fisher Scientific, Waltham, MA,USA) and anti-NIN mouse monoclonal antibody (LSBio ...
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bioRxiv - Cell Biology 2019Quote: ... and then incubated at room temperature for 1 hour in secondary antibody solution which contained Alexa 488-conjugated anti-mouse antibody (1:200; Thermo Fisher Scientific), rhodamine phalloidin (1:50 ...
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bioRxiv - Biophysics 2020Quote: ... incubated with an anti-mouse antibody produced in goat and labeled with a goat anti-mouse antibody at 1:200 (Molecular Probes, tetramethylrhodamine, Invitrogen, T2762) for 45 min at 37°C ...
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bioRxiv - Cell Biology 2019Quote: ... Caveolin-1 rabbit polyclonal antibody (# PA1-064) and anti-pFAK (Y397) rabbit monoclonal antibody (# 44624) obtained from Thermo Fisher (Grand Island, NY).
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bioRxiv - Immunology 2020Quote: ... They were then exposed at room temperature first for 1 h to primary antibodies in blocking buffer and then for 1 h to Alexa Fluor–conjugated secondary antibodies (Thermo Fisher Scientific) and Hoechst 33342 in blocking buffer ...
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bioRxiv - Microbiology 2021Quote: ... Coverslips were blocked for 1 hour with PBS and 1% BSA before 1 hour incubation with the appropriate primary antibody and followed by 1 hour with Alexa-fluor conjugated secondary antibody 488 (Invitrogen 1/500). Coverslips were mounted using Vectashield Hardset Mounting Medium with DAPI (Vector laboratories) ...
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bioRxiv - Microbiology 2020Quote: ... Alexa Fluor™ 488/568/647-conjugated antibodies against mouse IgG and rabbit IgG were used as secondary antibodies (1:500; Invitrogen, USA).
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bioRxiv - Biochemistry 2020Quote: ... Cells were then stained with M2 FLAG primary antibody (SigmaAldrich, F1804) and AlexFluor 594-conjugated anti-mouse goat antibody (ThermoFisher, A-11005) using the same conditions ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with biotin-conjugated anti-PE antibody together with antibodies for surface markers for flow cytometric analysis using AttuneNxT (Thermo Fisher Scientific) and FlowJo 10.5.2 (BD Biosciences).
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bioRxiv - Cell Biology 2019Quote: ... Binding of the primary antibody was detected by incubation with Alexa Fluor 488-conjugated goat anti-mouse or secondary antibody (Invitrogen Molecular Probes). The sexual stages were highlighted by double-labelling with rabbit antibodies directed against Pfs230 ...
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bioRxiv - Molecular Biology 2019Quote: ... and rabbit anti-D5R antibody and donkey anti-rabbit secondary antibody tagged with Alexa Fluor™ 488 (#R37118, ThermoFisher Scientific Waltham, MA). The tissues were counterstained with wheat germ agglutinin (WGA ...
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bioRxiv - Biochemistry 2019Quote: ... proteins transferred to nitrocellulose were detected using primary rabbit or mouse antibodies and visualized with appropriate secondary antibodies conjugated to IRDye (Thermo Fisher Scientific). Quantification was performed with Image J software (NIH).
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bioRxiv - Bioengineering 2019Quote: ... 5×106 cells were labeled with a 1:100 dilution of chicken anti-c-myc antibody or mouse anti-V5 antibody (Thermo Fisher Scientific) for 15 minutes at room temperature ...
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bioRxiv - Cancer Biology 2020Quote: ... Chromatin fragments were immunoprecipitated overnight using 1 μg antibody of SOX11-PAb antibody and 20 μl Protein A UltraLink® Resin (Thermo Scientific) beads per 10×106 cells ...
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bioRxiv - Cell Biology 2021Quote: ... Membranes were incubated with primary antibodies and HRP-conjugated secondary antibodies and then blotted with substrates or Super Signal West Femto Maximum Sensitivity Substrate (Thermo Fisher Scientific). Imaging was performed with the Image Lab software ...
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bioRxiv - Genomics 2021Quote: ... Then these sliced samples were washed with 0.05 % Tween 20 (Nacalai) 3 times and incubated with secondary antibodies (Alexa Fluor 488- and 561-conjugated antibodies, Life Technologies, 1:500) diluted with 0.05 % Tween 20 for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2021Quote: ... Secondary antibodies were used at the same concentration as their respective primary antibodies and included donkey anti-rabbit 568 (ThermoFisher, cat# A10042), donkey anti-chicken 647 (Millipore ...
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Enhancer Remodeling Promotes Tumor-Initiating Activity in NRF2-Activated Non-Small Cell Lung CancersbioRxiv - Cancer Biology 2020Quote: ... which were prebound to anti-mouse IgG-conjugated Dynabeads (anti-H3K27ac antibody) or anti-rabbit IgG-conjugated Dynabeads (the rest of the antibodies) (Thermo Fisher Scientific). Precipitated DNA was de-crosslinked ...
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bioRxiv - Neuroscience 2021Quote: ... and then immunostained for 16 h at 4°C with primary antibodies followed by Alexa Fluor® 568- or 633-conjugated secondary antibodies (Molecular Probes) for 1 h at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... cells were washed once in PB and incubated with fluorescent-conjugated secondary antibody at 1:500 dilution in PB-2% BSA for 1 hour (Alexa Fluor 405/488/568– goat anti-guinea pig antibody for EbpC and goat anti-rabbit antibody for EbpA [Invitrogen, Inc., USA]). Cells were then washed once in PB and resuspended in 1 mL PB ...
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bioRxiv - Pathology 2021Quote: ... blocked with 1% BSA for 1 hr at room temperature (RT) and stained with anti-GFP antibody (1:1000; Abeam #ab6556) or anti-V5 tag antibody (1:500; Invitrogen #37-7500) for 2 hr at RT ...
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bioRxiv - Biophysics 2021Quote: ... This step was performed in order to break the existing cadherin junctions and allow the blocking antibodies (anti N-Cadherin antibody: LEAF™ Purified anti-human CD325, #350804, Biolegend, USA; anti E-Cadherin antibody: CD324 #16-3249-82, Invitrogen, USA) to bind to their respective epitopes ...
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bioRxiv - Microbiology 2021Quote: ... Cells were incubated with mouse mAb SARS-CoV-2 nucleocapsid antibody (SinoBiological, 1:100) and rabbit Claudin 7 polyclonal antibody (ThermoFisher, 1:200) overnight at 4°C ...
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bioRxiv - Physiology 2020Quote: ... Membranes were blocked with casein before primary antibodies were added (1:3000 dilution of mouse monoclonal antibody against the V5 tag (ThermoFisher MA5-15253)) in a casein buffer containing Tween ...
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bioRxiv - Immunology 2020Quote: ... at 4°C and bound antibodies were detected by fluorescently labelled anti-rat AlexaFluor 488 and anti-rabbit IgG AlexaFluor 647 antibodies (Thermo Fisher Scientific). Nuclei were counterstained with DAPI (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated with primary antibodies followed by secondary antibodies conjugated to Alexa fluorophore and then mounted with ProLong Glass Antifade Mountant (P36982, Thermo Fisher Scientific). Nuclei were stained with DAPI (11034-56 ...
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bioRxiv - Cell Biology 2021Quote: ... Lysates were then incubated with anti-IRE1 antibody or anti-GST antibody and precipitated using protein A-agarose (Invitrogen, Carlsbad, CA, USA). Precipitated proteins were separated by 10% sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) ...