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Citations for Thermo Fisher :
4601 - 4650 of 10000+ citations for SpectraDye Antibody Labeling Kit IR800 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Fixed MDCK cells were incubated with primary antibodies listed below and then incubated further with Alexa Fluor 488-conjugated antibody (Thermo Fisher Scientific). Fixed A498 cells were incubated with Alexa Fluor 594-conjugated phalloidin (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: ... Rabbit antibody to pSMAD 1/5/8 (1:100; CST 9516) and mouse antibody to beta-amyloid (1:100; Invitrogen 13-200) were diluted in the same 3% blocking buffer and incubated overnight at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... CP was detected using anti-CPNTD MLB1 antibody followed by incubation with secondary antibody (Alexa Fluor 488-conjugated goat anti-rabbit, Thermo Fisher, A21441). Nuclei were counter-stained with Hoechst ...
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bioRxiv - Developmental Biology 2022Quote: ... Binding of the primary antibody was visualized with secondary donkey anti-rabbit antibody conjugated with AlexaFluor® 555 (1:1000, Molecular Probes, Invitrogen). UB branches and the collecting ducts were stained with FITC-conjugated DBA (1:500 ...
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bioRxiv - Microbiology 2022Quote: ... cells were permeabilized as described above and sequentially stained with primary rabbit α-GFP antibodies (1:250; MBL) and secondary goat α-rabbit Alexa Fluor 488 antibodies (2 µg/ml; Invitrogen) for 1h at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... They were then washed with PBS three times and incubated with secondary antibody: either Alexa Fluor 488 Goat Anti-Rabbit IgG (H+L) antibody (Thermofisher; A-11008) or Alexa Fluor 647 Donkey anti-Rabbit IgG (H+L ...
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bioRxiv - Molecular Biology 2023Quote: ... The primary antibody was then detected by an Alexa Fluor™ 488-conjugated goat anti-mouse secondary antibody (Invitrogen, Cat# A-11001), which was diluted 1:500 in blocking solution (PBS containing 1% FCS [Bioconcept ...
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bioRxiv - Neuroscience 2023Quote: ... Sections were then incubated overnight at 4 °C with the primary antibody rabbit anti-CaMKIIα polyclonal antibody (1:1000, Invitrogen PA5-84083), mouse anti-parvalbumin (1:1000 ...
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bioRxiv - Microbiology 2023Quote: ... and the endogenously C-terminal HA-tagged cytochromes were visualized with a Roche Rat anti-HA monoclonal 3F10 primary antibody and FITC-conjugated Donkey anti-Rat 2° antibody (Invitrogen Life Technologies A18746). Images were taken on DIC/brightfield ...
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bioRxiv - Microbiology 2023Quote: ... the parasite mitochondrion was visualized using a polyclonal Rabbit anti-HSP60 antibody (Novus NBP2-12734) and AlexaFluor 647-conjugated Goat anti-Rabbit 2° antibody (Invitrogen Life Technologies A21244), the nucleus was stained with ProLong Gold Antifade Mountant with DAPI (Invitrogen Life Technologies P36931) ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were immunostained with goat anti-calbindin (1:300) as the primary antibody and anti-goat Alexa Fluor 488 (1:200) as the secondary antibody (Invitrogen, Eugene, OR). Sections were counterstained with 4’,6-diamidino-2-phenylindole (DAPI ...
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bioRxiv - Neuroscience 2022Quote: ... Transferred blots were blocked in 5% non-fat dry milk and subjected to primary antibody and HRP conjugated secondary antibody before visualization with Pierce SuperSignal™ West Pico PLUS Chemiluminescent Substrate (Thermo Fisher). Western blot images were obtained using the ChemiDoc Imaging System (Bio-Rad Laboratories).
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bioRxiv - Neuroscience 2022Quote: ... Sections were washed to remove primary antibody (PBS 0.1 M) and then incubated with fluorescently labelled secondary antibody (goat anti-rat Alexa Fluor 594; Invitrogen - Molecular Probes A21211) in PBST containing 2 % (v/v ...
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bioRxiv - Microbiology 2022Quote: ... Cells from infectivity and replications assays were incubated 1 hour at room temperature with anti-cytosolic tryparedoxin peroxidase (cTXNPx) primary antibody (Pineyro et al., 2008) and 30 minutes with Alexa Fluor 488 anti-rabbit IgG secondary antibody (Invitrogen, Eugene OR). Parasites from the epimastigogenesis experiment were attached to poly-L-lysine-coated slides and treated as monolayers ...
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bioRxiv - Microbiology 2022Quote: ... HBLAK cells grown on membranes were incubated with the following primary antibodies: rabbit anti-TLR-2 polyclonal antibody (10 µg/ml, PA5-20020, Thermo Fisher Scientific), rabbit anti-TLR-4 polyclonal antibody (5 µg/ml ...
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bioRxiv - Neuroscience 2022Quote: ... anti-rabbit, and anti-rat antibodies conjugated with Alexafluor dyes (Alexa 546, and Alexa 633) were used as secondary antibodies (ThermoFisher, 1:500). Immunolabeled sections were mounted on glass slides in PBS buffer with 70% glycerol for confocal LSM scanning ...
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bioRxiv - Biochemistry 2024Quote: ... Blots were then incubated with Odyssey blocking buffer for 30 min at room temperature and incubated with primary antibodies (1:1000 dilution rabbit anti-PTER antibody (Invitrogen, PA5-20750), 1:5000 dilution rabbit anti-β-actin antibody (Abcam ...
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bioRxiv - Cell Biology 2024Quote: ... They were then incubated overnight at 4 °C with the primary antibody (anti-TMEM173/STING antibody (OT14H1),1:100 dilution) (Invitrogen, MA,USA). After they were rinsed with PBS-T ...
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Increased dosage of wild-type KRAS protein drives KRAS-mutant lung tumorigenesis and drug resistancebioRxiv - Cancer Biology 2024Quote: The following secondary antibodies were used: Donkey or Goat Alexa488-or Alexa568 or Alexa594-or Alexa 647-conjugated secondary antibodies (ThermoFisher, Molecular Probes) (A10037 ...
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bioRxiv - Microbiology 2024Quote: ... coverslips were washed three times for 10 min in PBS and subsequently incubated with the secondary antibody goat anti-rabbit alexa-488 antibody (Thermo Fisher/Invitrogen) (1:300) ...
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bioRxiv - Genomics 2024Quote: ... The cells were incubated in the diluent buffer (ab64211) of the primary polyclonal Anti-CYP11B1 antibody (ab197908) and the TSH Receptor monoclonal antibody (4C1) (Invitrogen, MA5-16519) at a dilution of 1:200 for 1 h at 4°C ...
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bioRxiv - Neuroscience 2022Quote: ... neurons were blocked again for 30 min at room temperature and incubated with secondary antibodies (Alexa Fluor antibodies from Invitrogen, 1:500) for 1 hour at room temperature ...
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bioRxiv - Neuroscience 2023Quote: ... The secondary antibodies used were Alexa Fluor 633-conjugated goat anti-mouse antibody (Cat No. A-21050, 1:200, Thermo Fisher Scientific) and Alexa Fluor 488-conjugated goat anti-rabbit antibody (Cat No ...
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bioRxiv - Neuroscience 2023Quote: ... Immunohistochemistry was performed by incubating with primary antibodies (overnight, 4°C) and fluorophore-conjugated secondary antibodies (2 hr, room temperature; ThermoFisher, Jackson ImmunoResearch). Sections were mounted with VectaShield (VECTOR ...
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bioRxiv - Microbiology 2023Quote: ... Primary and secondary antibodies were added at indicated dilution (see antibodies section) for 10 min and each antibody was washed four times with PBS containing 0.5% TWEEN-20 (Fisher Scientific, Cat. # BP337) v/v (PBST ...
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bioRxiv - Neuroscience 2023Quote: ... sections were incubated with antibody solution containing 1:500 diluted secondary antibodies and 1:10,000 diluted Hoechst 33342 nuclear dye (Thermo Fisher Scientific, H3569), for 1-2 hours at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... coverslips were inverted onto 100 μl of antibody block with secondary antibodies (Alexa Fluor 488 anti-mouse, 1:200, A11029; Thermo Fisher Scientific) and DAPI (DNA ...
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bioRxiv - Neuroscience 2023Quote: ... And then incubate the brain sections in 2° antibodies (1:700 for Dylight-/Alexa-conjugated antibodies made in donkey purchased from Thermo Fisher Scientific) in 5% BSA/PBST and incubate for 2 hours at RT ...
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bioRxiv - Molecular Biology 2023Quote: ... Secondary antibody incubation was conducted using Alexa Fluor 488 and 546 coupled antibodies (Thermo Fisher Scientific; A-11008, A-11081; A-11001) at a 1:1000 dilution ...
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bioRxiv - Immunology 2023Quote: ... Flow cytometry was performed using a Myltenyi VYB cytometer with MEM-G/9 antibody (anti-HLA-G antibody ; Thermo Fisher #MA1-19014), 3D12 (anti-HLA-E antibody ...
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bioRxiv - Genomics 2023Quote: ... for 1 hour, followed by incubation with primary antibodies (overnight, 4°C) and secondary antibodies (1-2 hours, from Jackson ImmunoResearch or ThermoFisher Scientific). The slides were then mounted with coverslips using VECTASHIELD (Vector Labs ...
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bioRxiv - Biophysics 2023Quote: ... ATP synthase dimerization state was probed using an anti-ATP synthase primary antibody (Rak & Tzagoloff, 2009) (1:1000) and anti-rabbit IgG secondary antibody (Thermo Fisher Scientific). Supercomplex formation was assessed using an anti-Cox1p (CIV ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were washed three times and incubated with secondary antibody (anti-mouse or anti-rabbit (depending on primary antibody host) conjugated to Alexa Fluor™ 568 (1:800, donkey, Invitrogen, UK)) for ∼4 hrs at RT in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... RRID:AB 10988206) were incubated along with OCT2 antibodies and revealed with Alexa 555-conjugated secondary antibodies (Thermo Scientific, Cat# A-31572, RRID:AB_162543).
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bioRxiv - Neuroscience 2023Quote: ... as the primary antibody and an anti-rabbit IgG antibody conjugated with Alexa Fluor 488 plus (goat, 1/400, #A32731, Thermo Fisher Scientific) as a secondary antibody ...
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bioRxiv - Neuroscience 2023Quote: ... Hair cells were labeled with an anti-Myosin 7A primary antibody (#25-6790 Proteus Biosciences, 1:500) followed by application of a donkey anti-rabbit AF488 secondary antibody (Invitrogen, 1:500). Samples were additionally labeled with Phalloidin ...
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bioRxiv - Biophysics 2023Quote: ... the samples were rinsed three times with 3% BSA and additionally incubated with a secondary antibody (Alexa Fluor 488 goat anti-rabbit antibody (A11008, Thermo Fisher Scientific)) in 3% BSA with a 1:200 ratio at room temperature for 2 hrs ...
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bioRxiv - Animal Behavior and Cognition 2022Quote: ... and placed in a fluorophore-conjugated (Alexa FluorTM Plus 488) secondary antibody solution [1:300 dilution of donkey anti-Rabbit secondary antibody (Thermo Fisher, AB_2762833) in PBS] for 24 hr ...
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bioRxiv - Developmental Biology 2023Quote: ... The 2° antibody used was Alexa Fluor® 488 Goat antiMouse IgG1 Cross-Adsorbed Secondary Antibody (1:100,ThermoFisher Scientific, Cat. A21121)
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bioRxiv - Developmental Biology 2022Quote: ... sections were incubated with antibody solution containing 1:500 diluted secondary antibodies and 1:10,000 diluted Hoechst 33342 nuclear dye (Thermo Fisher Scientific, H3569), for 1-2 hours at RT ...
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bioRxiv - Microbiology 2023Quote: ... 1:2,000 rat α-LAMP1 (Developmental Studies Hybridoma Bank) primary antibodies and 1:500 Alexa 488-conjugated goat α-rabbit and Alexa594-conjugated goat α-rat secondary antibodies (ThermoFisher) in blocking buffer ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were then again incubated with p65 primary antibody (1:1000) overnight and successively incubated with secondary antibody tagged with Alexa Fluor IgG 555 (#A32732, Thermo Fisher Scientific) as per abovementioned protocol ...
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bioRxiv - Neuroscience 2023Quote: ... The sections were rinsed 2 times with PBS and then incubated for 2 hours in 1:200 Cy5-conjugated donkey anti-rabbit secondary antibody or 1:200 Cy5-conjugated donkey anti-goat secondary antibody (Thermo Fisher Scientific). The sections were rinsed 2 times with PBS and mounted in ProLong diamond antifade mounting media (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... The blot was further evaluated for G6PD expression using primary antibody against G6PD (Rabbit mAb # A11234) and the counter labelling anti-rabbit HRP secondary antibody (Invitrogen Catalog # 31460). Chemiluminescence was capture in iBright (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... Fc-fused antibodies and full-length antibodies and their respective complexes with cSC were purified using Capture Select IgA affinity resin (Thermo Scientific: 194288010) and eluted using 0.1M glycine at pH=3 ...
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bioRxiv - Neuroscience 2023Quote: ... This was followed by overnight incubation with primary antibody (see below) and eventually conjugated to a fluorophore with the secondary antibody (1:500, ThermoFisher, Alexa Fluor). The sections were incubated in primary antibody for 24LJh at 4 °C ...
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bioRxiv - Synthetic Biology 2024Quote: ... were washed three times in cold flow buffer and then the secondary antibody (Goat anti-Human IgG H+L Cross-Adsorbed Secondary Antibody, Alexa Fluor™ 488, ThermoFisher Scientific) at a dilution of 1:500 was added ...
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bioRxiv - Microbiology 2023Quote: SARS-CoV-2 Nucleocapsid antibody (produced in mouse) as a primary antibody was procured from (MA5-35943, Invitrogen, 1:1000-1:10,000), Rabbit phospho-p38 MAPK and total p38 MAPK Antibody were procured from (9211S and 9212S ...
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bioRxiv - Molecular Biology 2023Quote: ... Primary antibodies were detected with HRP-conjugated secondary antibodies (rabbit IgG-, Protein A-, or mouse IgG-conjugated with HRP, Thermo Fisher Scientific) and ECL detection reagent (Amersham) ...
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bioRxiv - Immunology 2023Quote: ... For hamster samples a SARS-CoV-2 Nucleocapsid antibody (SinoBiological #40588) followed by a goat anti-rabbit IgG Alexa Fluor 488 antibody (ThermoFisher Scientific #A32731) was used (6) ...