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Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations for SpectraDye Antibody Labeling Kit IR800 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... Fixed cells were then incubated with primary antibodies for 1 hour and then for a further 1 hour with appropriate secondary antibodies conjugated with a fluorophore (Molecular Probes). The antibodies used and respective dilutions are displayed in Supplementary Table 1 ...
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bioRxiv - Cancer Biology 2022Quote: ... Conjugated antibodies were then validated signal by IMC in suspension mode (Helios) with antibody capture beads (AbC Total compensation beads, Thermo Fisher). To confirm conjugation did not alter antibody binding efficiency ...
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bioRxiv - Neuroscience 2022Quote: ... primary antibody followed by incubation of horseradish peroxidase-linked goat anti-rabbit or anti-mouse secondary antibody (1: 5,000, Thermo Fisher Scientific). Antibody binding was detected using the enhanced chemiluminescence immunoblotting detection system (Perkin Elmer LLC WESTERN LIGHTNING PLUS-ECL ...
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bioRxiv - Neuroscience 2022Quote: ... followed by secondary antibody-staining with Alexa 647-tagged goat anti-guinea pig antibody (catalog no. A21450, Thermo Fisher Scientific, USA), after which the sections were rinsed ...
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bioRxiv - Neuroscience 2022Quote: ... Detection of primary antibodies was achieved using secondary antibodies conjugated to Goat anti-mouse Alexa488 (1:400, Thermo Fisher, Cat#A28175), Goat anti-rabbit Alexa488(1:400 ...
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bioRxiv - Genetics 2022Quote: ... antibodies followed by AlexaFluor 488 anti-rabbit and AlexaFluor 568 anti-mouse secondary antibodies (Both at 1:1000; Thermo Fisher Scientific). To visualize the GR8A protein ...
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bioRxiv - Cell Biology 2022Quote: ... in PBS with 0.05% Tween-20 (PBST) and incubated in primary antibody and respective secondary antibody (Alexa Fluor; Thermo Fisher Scientific) in blocking buffer diluted 1:5 with PBST ...
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bioRxiv - Neuroscience 2022Quote: ... The expression of Kir2.1EGFP was assessed with anti-GFP antibody (rat monoclonal Ab from Nacalai, Japan, GF090R; the secondary antibody, 488-anti Rat IgG from Invitrogen, A11006) (Mizuno et al. ...
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bioRxiv - Cell Biology 2022Quote: ... the membranes were incubated with anti-Sgs1 antibody (dilution 1:500) and anti-V5 antibody (dilution 1:1000, Thermo Fisher Scientific) at 4°C overnight ...
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bioRxiv - Cell Biology 2022Quote: ... the samples were washed three times in PBST and incubated for one hour at room temperature with the fluorescent secondary antibody (Goat anti-mouse or Goat anti-rabbit antibodies conjugated with either Alexa488 or Alexa555, 1:400 dilution, Life technologies) in the same blocking buffer ...
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bioRxiv - Immunology 2020Quote: ... The lysates were then resolved by SDS-PAGE and immunoblotted with indicated antibodies (Wapl and V5-tag antibodies were purchased from Thermo Fisher Scientific #PA5-38024 and #R960-25 ...
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bioRxiv - Neuroscience 2019Quote: ... brain sections were washed 3 times with PBS-T and incubated with appropriate fluorophore-conjugated or streptavidine-fluorophore conjugated (for APOE biotinylated antibody) secondary antibodies (1:500, Life Technologies) together with nuclear stain Hoechst 33342 (1:2000 ...
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bioRxiv - Molecular Biology 2020Quote: ... Anti-PrimPol antibody (Table 1) was coupled to magnetic M-270 Epoxy Dynabeads® (7.5 μg antibody/ mg beads; Life Technologies), and IPs were performed following manufacturer instructions ...
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bioRxiv - Cell Biology 2021Quote: ... Western blots were carried out using iBind with 1 ug/ml primary antibodies and 1 ug/ml secondary antibodies following iBind protocol (Invitrogen/ThermoFisher).
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were visualized using donkey anti-rabbit antibody conjugated with Alexa Fluor Plus 555 (1:1000, #A32794, Thermo Fisher Scientific) and donkey anti-goat antibody conjugated with Alexa Fluor Plus 488 (1:1000 ...
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bioRxiv - Systems Biology 2020Quote: ... Visualization of antibody signals was achieved using HRP-conjugated secondary antibodies and the chemiluminescence substrate SuperSignal West Pico (Thermo Fisher Scientific). Blots were imaged and quantitatively analyzed using a Chemidoc MP imager (Biorad) ...
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bioRxiv - Neuroscience 2022Quote: The primary antibodies were as follows: mouse anti-HA tag (2-2.2.14) antibody (1:1000; Thermo Fisher Scientific, cat. no. 26183); rabbit anti-HA tag (C29F4 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... After 2 washes to remove any excess antibody cells were stained with anti-Rabbit Alexa-546 antibody (Life technologies, Cat#A11035). After 3 washes to remove any non-specific antibody nuclei were stained using Hoechst at 1:50,000 dilution and imaged on a Nikon Ti Eclipse automated microscope ...
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bioRxiv - Microbiology 2022Quote: ... Cells were washed three times with PBS and then incubated with secondary antibody (1:1000 goat anti-rabbit antibody or goat anti-mouse antibody conjugated to Texas Red and 1:1000 goat anti-mouse antibody conjugated to Alexa Fluor 647 (Life Technologies) and 1:1000 donkey anti-goat antibody conjugate to Texas Red (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... The secondary anti-mouse and anti-rabbit horseradish peroxidase-conjugated antibodies and the Alexa Fluor 488- and 555-congugated antibodies were from Thermo Fisher Scientific.
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bioRxiv - Biophysics 2020Quote: ... incubated with an anti-mouse antibody produced in goat and labeled with a goat anti-mouse antibody at 1:200 (Molecular Probes, tetramethylrhodamine ...
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bioRxiv - Neuroscience 2019Quote: ... Secondary antibody staining was performed as per the primary with the appropriate AlexaFluor 488 conjugated secondary antibody (Life Technologies Ltd, UK) at a concentration of 1:50 ...
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bioRxiv - Developmental Biology 2020Quote: ... primary antibodies were detected using appropriate secondary antibodies coupled to Alexa-Fluor 488 and Alexa Fluor 594 (1:100; Molecular Probes) and diluted in PBTr for 2 days at 4°C.
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bioRxiv - Immunology 2019Quote: pOV8/Kb levels were determined by incubating cells for one h on ice with the 25-D1.16 antibody followed by one h on ice with secondary goat anti-mouse antibody conjugated to Alexa Fluor 647 (Molecular Probes). Cellular fluorescent proteins and Alexa Fluor 647 levels were determined on a FACS Calibur cytofluorograph (Beckton Dickinson ...
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bioRxiv - Pathology 2020Quote: ... The binding sites of the primary antibodies were revealed with Alexa Fluor-594 goat anti-rabbit and Alexa Fluor-488 goat anti-mouse secondary antibodies (Invitrogen, USA). Nuclei were stained with 4’ ...
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bioRxiv - Microbiology 2020Quote: ... The resulting blots were probed with a sdAb as primary antibody and an HRP-linked 6x-His Tag antibody (Thermofisher, HIS.H8) as the secondary antibody ...
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bioRxiv - Pathology 2020Quote: ... The secondary antibodies used to reveal LC3 and LAMP2 were Alexa 594-labeled anti-rabbit secondary antibody and Alexa 546-labeled anti-mouse secondary antibody (Invitrogen, USA), respectively ...
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bioRxiv - Molecular Biology 2020Quote: ... Cells were washed thrice in PBS containing 0.05% Tween 20 and incubated with secondary antibody (anti-mouse antibody conjugated to Alexa 647 or Alexa 546, Thermo Fisher Scientific) at 1:4000 dilution and GFP nano-booster (Chromotek ...
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bioRxiv - Microbiology 2021Quote: ... or rabbit TgALD1 antibody (1:200, kind gift from Dr. Kentaro Kato) and anti-rabbit HRP antibodies (Thermo Fisher, 1:10000) followed by development of signal with West Pico Plus Chemiluminescent Substrate (Thermo Fisher) ...
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bioRxiv - Synthetic Biology 2021Quote: ... as the primary antibody at a 1:100 dilution and anti-mouse IgG Alexa Fluor 488 as the secondary antibody (A11029, Life Technologies) at a 1:100 dilution ...
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bioRxiv - Microbiology 2020Quote: ... and rabbit anti-SERA5 (1:500) (54) primary antibodies in combination with appropriate fluorophore-coupled secondary antibodies (1:1,000; Thermo Fisher Scientific). Stainings with Lysosensor Blue DND-167 (Thermo Fisher Scientific) ...
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bioRxiv - Molecular Biology 2020Quote: The rabbit α-NOCT polyclonal antibody was produced in rabbits inoculated with MBP-NOCT (1-431) (Thermo Fisher custom antibody services). This MBP-NOCT antigen (1-431 ...
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bioRxiv - Neuroscience 2019Quote: ... Sections were incubated in primary antibodies for 48 h at 4°C and secondary antibodies conjugated to AlexaFluor-488 or AlexaFluor-594 (Life Technologies) for 2 h at room temperature ...
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bioRxiv - Immunology 2021Quote: ... for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088, Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2019Quote: ... The membranes were incubated with the antibodies indicated above and visualized with horseradish peroxidase–conjugated secondary antibodies using the ECL Western blotting system (Thermo Scientific).
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bioRxiv - Cell Biology 2019Quote: ... The membranes were washed for 15 min in TBST and then incubated for 1 h with HRP-conjugated secondary antibodies (1:5,000; Pierce Antibodies; Thermo Fisher Scientific) in TBST at RT ...
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bioRxiv - Molecular Biology 2019Quote: ... either fluorophore-coupled secondary antibodies or HRP-conjugated secondary antibodies and WesternBright Chemiluminescent Substrate (Biozym Scientific) or SuperSignal West Pico Chemiluminescent Substrate (Life Technologies) were used ...
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bioRxiv - Developmental Biology 2019Quote: ... embryos were incubated with the secondary antibodies (1:1000, Goat anti-Mouse IgG secondary antibody, Alexa Fluor 488, Life technologies, USA) for 2 h at RT ...
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bioRxiv - Cell Biology 2019Quote: ... Binding of the primary antibody was detected by incubation with Alexa Fluor 488-conjugated goat anti-mouse or secondary antibody (Invitrogen Molecular Probes). The sexual stages were highlighted by double-labelling with rabbit antibodies directed against Pfs230 ...
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bioRxiv - Cell Biology 2019Quote: ... Aortae were perfused in situ with PBS and then perfusion-fixed with 4% paraformaldehyde prior to staining using specific primary antibodies and AlexaFluor568-conjugated secondary antibodies (Life Technologies) or with AlexaFluor568-phalloidin (ThermoFisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: ... and secondary antibody solution was added (1-10ug/ml) (Alexa Fluor 488 Donkey anti-mouse IgG secondary antibody, ThermoFisher, A-21202). DAPI staining was used for nuclei ...
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bioRxiv - Cell Biology 2021Quote: ... the sections were incubated in secondary antibodies (Alexa Fluor 488-, 555-, 594-, 647-conjugated secondary antibodies, or anti-mouse IgG isotype-specific secondary antibodies; 1:500; Life Technologies, Carlsbad ...
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bioRxiv - Immunology 2021Quote: ... and incubated with the secondary antibody (Alexa-Fluor 647- or Alexa-Fluor 405-conjugated goat anti-mouse Ig antibodies; Invitrogen, France) for 45 min at room temperature ...
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bioRxiv - Physiology 2020Quote: ... Secondary antibody at a 1/1000 dilution (Alexa Fluor 594 goat anti-rabbit antibody or Alexa Fluor 488 goat anti-mouse antibody, Thermo Fisher) in blocking buffer also containing Alexa Fluor-405 phalloidin (ThermoFisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: GFP was visualized using rabbit polyclonal or mouse monoclonal anti-GFP primary antibodies and Alexa 647-labelled secondary antibodies (Molecular Probes/Invitrogen) directed against rabbit or mouse ...
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bioRxiv - Physiology 2021Quote: ... Alex Fluor 546 donkey anti-rabbit secondary antibodies and Alex Fluor 647 goat anti-rat secondary antibodies (1:500; Molecular Probes) for 1 hour at room temperature ...
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bioRxiv - Biochemistry 2021Quote: ... or a major outer membrane protein (MOMP) antibody (1:1000, Novus Bio) and an appropriate AlexaFluor secondary antibody (1:200, ThermoFisher Scientific). Counterstaining was performed with Hoechst stain ...
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bioRxiv - Neuroscience 2021Quote: ... 13) anti-Lectin antibody (#DL-1174, Vector, Burlingame, CA, USA) and anti-CD144 antibody (#14-1441-82 Invitrogen, Carlsbad, CA, USA) to label vascular and endothelia.
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bioRxiv - Neuroscience 2021Quote: ... an anti-P2X3 antibody (1:1000, Alomone APR016, anti-rabbit polyclonal) and an Alexa-488 conjugated secondary antibody (1:1000, Invitrogen A21206). The mean gray value of each DRG neuron was measured in ImageJ and a custom-made R toolkit (https://github.com/amapruns/Immunohistochemistry_Analysis ...
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bioRxiv - Cell Biology 2022Quote: ... Australia) primary antibody and a donkey anti-mouse IgG (H+L) secondary antibody conjugated to Alexa Fluor 568 (A10037; ThermoFisher, France), essentially as described (28) ...