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Citations for Thermo Fisher :
4301 - 4350 of 6549 citations for 6 Chloro N methoxy N methyl nicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... After 6 hours the medium containing transfection reagents was replaced by FluoroBrite DMEM medium (Thermo Fisher, A1896701), supplemented with 10% (v/v ...
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bioRxiv - Biochemistry 2023Quote: ... and 2mM DTT) for 30 min at 4 °C and analyzed on 6% TBE gels (Life Technologies) at 4 °C ...
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bioRxiv - Microbiology 2023Quote: ... The pellet was dissolved in 50 μL of 6 M guanidine hydrochloride (GuHCL, Thermo Scientific™, AAJ6078622) and placed on the shaker for 20 min at 1000 rpm ...
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bioRxiv - Bioengineering 2023Quote: ... Bulk GBM cell lines (GBM-43, GBM-6) were harvested using 0.25% Trypsin-EDTA (Thermo Fisher Scientific) and passaged less than 30 times.
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bioRxiv - Neuroscience 2023Quote: The iPSCs and ESCs were maintained on 6-well plates coated with Geltrex (#A1413302, Thermo Fisher Scientific). For the initial 24 hours following seeding ...
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bioRxiv - Molecular Biology 2023Quote: ... The melt curve step was carried out in a QuantStudio 6 Real-Time PCR system (Applied Biosystems). The PCR process involved UDG (Uracil-DNA Glycosylase ...
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bioRxiv - Neuroscience 2023Quote: ... 6 and 8 weeks of maturation were loaded with 2.5 µM Fluo-3-AM (Molecular Probes, #F1242) dissolved in differentiation medium for 30 min at 37 °C and at 5% CO2 ...
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bioRxiv - Bioengineering 2023Quote: ... iPSCs were cultured in a 6-well plate coated with 0.5 mg Matrigel™ (354230, Fisher Scientific) in complete mTeSR plus media (100-0276 ...
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bioRxiv - Cancer Biology 2023Quote: ... Transcript levels were measured with PowerUp SYBR Green Master Mix in QuantStudio 6 Flex System (Applied Biosystems). Initial activation was performed at 95°C for 20 s ...
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bioRxiv - Cancer Biology 2023Quote: ... Amplification was carried out in a QuantStudio™ 6 Flex Real-Time PCR System (#4485691, Applied Biosystems) under the following conditions ...
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bioRxiv - Cancer Biology 2023Quote: ... qPCR analysis was conducted on a QuantStudio 6 Flex Real-Time PCR System (4485697; Thermo Fisher Scientific) with Luna Universal qPCR Master Mix (M3003X ...
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bioRxiv - Biophysics 2023Quote: ... Cell nuclei were stained with 4’,6’-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng/ml; Molecular Probes). Stained sections were imaged using epifluorescence and deconvolution epifluorescence microscopy on an Olympus IX83 ...
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bioRxiv - Cell Biology 2023Quote: ... Each sample consisting of 6 lumbar NP tissues was pooled and stored in RNAlater® Reagent (Invitrogen) at 80°C until isolation ...
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bioRxiv - Molecular Biology 2024Quote: ... Cells were seeded onto 6-well plates forward transfected with Lipofectamine™ RNAiMAX transfection reagent (Invitrogen, # 13778150) according to manufacturer′s instructions using 30 pmol of siRNA for 72h at 37°C before harvesting ...
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bioRxiv - Biophysics 2024Quote: ... pH 8.0) from acetone powder and centrifuged in a Fiberlite F14 6×250 LE rotor (ThermoFisher Scientific) at 10,000 rpm for 30 min at 4 ºC ...
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bioRxiv - Bioengineering 2024Quote: ... 6-well cultures were transiently transfected with 7.32 ug of plasmid per well using Lipofectamine 3000 (ThermoFisher). 24 hrs after transfection ...
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bioRxiv - Genomics 2024Quote: ... Sections were stained with DAPI (4 ',6' -diamidino-2-phenylindole) and mounted in Prolong Gold (Life Technologies). Imaging was performed on a Nikon Eclipse Ti Confocal at 20X objective and processed using Nikon Elements-AR ...
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bioRxiv - Molecular Biology 2024Quote: 2.5-3.0 × 105 cells were seeded in 6-well dish and reverse transfected using Lipofectamine RNAiMAX (Invitrogen) and 60 pmol siRNA following the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2024Quote: ... Other primary antibodies used for immunofluorescence were mouse anti-acetylated tubulin (clone 6-11B, 32270, Thermo Fisher) at 1:10000 ...
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bioRxiv - Cancer Biology 2024Quote: ... RNA samples were then treated with 4-6 units of Turbo DNase (Thermo Fisher Scientific, cat# AM2238) at 37 °C for 30 min to remove the bulk of plasmid DNA contamination ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were cultured in 6-well plates precoated with 120-180 µg/mL Geltrex (Fisher Scientific, A1413302) in Gibco™ Essential 8™ medium (E8 ...
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bioRxiv - Cancer Biology 2024Quote: ... All the qRT-PCR probes (mouse IL-6, IL-1β and CCL2) were purchased from Applied Biosystems. The relative RNA expression levels were normalized to the expression of mouse HPRT (Applied Biosystems ...
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bioRxiv - Microbiology 2024Quote: RT was performed on 6 µl of eluted RNA using MMLV reverse transcriptase (Invitrogen, Carlsbad, CA, USA) with dT17 primer (for polyadenylated transcripts) ...
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bioRxiv - Microbiology 2024Quote: ... was purchased and prepared according to the instructions with 6% (v/v) defibrinated sheep blood (Thermo Scientific) added ...
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bioRxiv - Cancer Biology 2024Quote: ... before they are moved back to ultra-low attachment 6 well plate (Fisher Scientific 07-200-601) with corresponding culture media ...
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bioRxiv - Immunology 2024Quote: ... 6-well culture plates) for 8 days at 37°C and 5% CO2 in RPMI1640 (Life technologies) supplemented with 10% FBS ...
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bioRxiv - Immunology 2024Quote: ... IL-6 was analyzed from blood serums by sandwich enzyme-linked immunosorbent assay (ELISA; Thermo Fisher Scientific) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2024Quote: HeLa cells (ATCC, #CCL-2) were plated in DMEM (Cellgro, #10-013CV) containing 6% FBS (Gibco, #26140) at 10,000 cells per well in 96-well tissue culture plates ...
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bioRxiv - Immunology 2024Quote: ... T cells from C57BL/6 mice were stimulated with coated anti-CD3 (Thermo Fisher, #16-0032-85) (10 μg/mL ...
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bioRxiv - Synthetic Biology 2024Quote: ... Mixtures were subsequently fractionated by electrophoresis in precast 6% polyacrylamide gels (Novex TBE Gels; Invitrogen, Waltham, MA) buffered with 0.5X TBE (45 mM Tris borate ...
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bioRxiv - Microbiology 2024Quote: ... hMDM nuclei were stained with 50 ng/ml 4’,6-diamidino-2- phenylindole (DAPI) (Invitrogen Cat. # D1306) for 7 min at room temperature ...
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bioRxiv - Plant Biology 2020Quote: ... on 4-16% (Figures 4A and 7) or 3-12% (Figures 4C and 6) NativePAGE gels (Life technologies). Cathode Running buffer (Life technologies ...
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bioRxiv - Neuroscience 2021Quote: ... Larval zebrafish at 6-7 dpf were paralyzed by immersion in 1 mg/ml alpha-bungarotoxin solution (Invitrogen) dissolved in external solution (in mM ...
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bioRxiv - Cancer Biology 2021Quote: Cells (1-1.5×10^6) were incubated with FACS sample buffer (DPBS, 0.5% FBS) and human FC Block (Invitrogen) for 20 minutes on ice followed by incubation at 4°C for 30 minutes with the following - antibodies ...
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bioRxiv - Cell Biology 2020Quote: ... pH 8.0) was reacted with a 6-fold molar excess of Alexa Fluor 647 NHS Ester (ThermoFisher, A20006). Excess dye was removed by Zeba Spin column equilibrated in 20 mM Hepes ...
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bioRxiv - Cell Biology 2020Quote: ... the cells were mounted in antifade mounting medium with 4′,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific) and visualized with IX81 confocal microscope (Olympus ...
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bioRxiv - Cell Biology 2020Quote: ... Becton Dickinson) was placed along the duct and tied together using 6-0-black-silk suture (Fisher Scientific), and the incision was closed by surgical sutures ...
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bioRxiv - Developmental Biology 2021Quote: ... 2,000 PαS cells were cultured in a 6-well plate with 20% FBS (mesenchymal stem cell-qualified, Gibco), 25 units/mL penicillin/streptomycin and 2 mM L-glutamine in alpha MEM (Gibco ...
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bioRxiv - Cell Biology 2022Quote: ... 750,000 cells per well in a 6-well plate were transfected using Lipofectamine 2000 (5 μL; Thermo Fisher) as needed ...
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bioRxiv - Neuroscience 2021Quote: ... in a 384 well plate on a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher, A28139). Each PCR cycle had the following conditions for denaturation ...
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bioRxiv - Molecular Biology 2021Quote: ... total RNA was extracted from 6 – 12 fully-grown oocytes using Arcturus Picopure RNA isolation kit (Thermo Fisher) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... All quantitative PCR reactions were performed using QuantStudio-6/ViiA7/7900HT Fast Real-Time PCR System (Applied Biosystems) using 96-well or 384-well plates ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were seeded in 6-well plates and transfected with CFTR plasmids using Lipofectamine 3000 (Thermo Fisher Scientific).
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bioRxiv - Molecular Biology 2021Quote: ... Supernatant was collected 6 days later and recombinant mAb was purified using protein A agarose (Thermo Fisher Scientific) according to the manufacturer’s protocol.
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bioRxiv - Molecular Biology 2021Quote: ... and mounted on slides with Prolong Diamond Antifade Mountant with 4′,6-diamidino-2-phenylindole (DAPI) (Molecular Probes). Imaging was performed using a Nikon A1 Confocal microscope.
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bioRxiv - Cell Biology 2022Quote: ... INS-1 cells were transfected in 6-well plates with 5 nM siRNA using Lipofectamine RNAiMAX (Invitrogen, 13778030) and typically analyzed after 96 hours ...
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bioRxiv - Cell Biology 2022Quote: ... and then subjected to real-time PCR analysis using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Biophysics 2019Quote: ... 4-(2-(6-(dibutylamino)-2-naphthalenyl)ethenyl)-1-(3-sulfopropyl)-,hydroxide (di-4-ANEPPS) was purchased from Invitrogen. It was dissolved in ethanol and added to the dried lipid film at a 12:1 lipid:probe molar ratio ...
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bioRxiv - Bioengineering 2019Quote: ... Masson’s trichrome or 4’,6-diamidino-2-phenylindole (DAPI) mounting media (ProLong® Gold Antifade Mountant, Thermo Scientific). Representative light micrographs were captured with the Olympus VS-110 Virtual Imaging System and fluorescent micrographs with a Zeiss Axioplan2 system.
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bioRxiv - Cell Biology 2019Quote: ... cultured neurons on 6-10 DIV were switched to pre-warmed Eagle’s minimum essential medium (EMEM, Thermo Fisher) supplemented with GlutaMax 1 hour before transfection ...