Labshake search
Citations for Thermo Fisher :
4401 - 4450 of 6549 citations for 6 Chloro N methoxy N methyl nicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cell Biology 2022Quote: HCT116 cells were cultured in a 6-well plate at 300,000 cells per well in 3 ml McCoy’s 5A medium (Gibco) supplemented with 10% heat inactivated FBS and with 90 UI/mL Penicillin ...
-
bioRxiv - Cancer Biology 2020Quote: ... followed by growth in the absence of IL-2 for 6 - 9 h in RPMI 1640 (Gibco, # 21875034) medium supplemented with 10% FBS (Gibco ...
-
bioRxiv - Molecular Biology 2021Quote: ... HL-1 cells in 6-well plates were transfected with 15 µL Lipofectamine 2000 reagent (Life Technologies 11668019) complexes with nucleic acid ...
-
bioRxiv - Neuroscience 2021Quote: ... App gene expression level was assessed using Taqman probe (FAM-Mm01344172_m1) on a QuantStudio 6 Flex (Applied Biosystems) and normalized to Gapdh.
-
bioRxiv - Neuroscience 2020Quote: ... according to the protocol below on a LightCycler 480 Instrument or the QuantStudio 6 Flex instrument (Applied Biosystems) in a 96-well format ...
-
bioRxiv - Microbiology 2020Quote: ... 1976) and maintained in 6% O2 and 5% CO2 and grown in sterile filtered RPMI 1640 media (Gibco) supplemented with 2 M HEPES ...
-
bioRxiv - Biochemistry 2021Quote: ... Coverslips were mounted on slides with proLong gold antifade reagent with 4’,6-diamidino-2-phenylindole (Invitrogen, USA), which was used to probe for the nucleus.
-
bioRxiv - Neuroscience 2020Quote: ... treated coverslips and incubated at 37°C for 2-6 hours in Dulbecco’s modified Eagle’s medium (F-12/DMEM; Invitrogen) supplemented with 1% fetal bovine serum (FBS ...
-
bioRxiv - Biochemistry 2021Quote: ... Supernatant was carefully aspirated and cells were resuspended in fresh media and plated in 6-cm plates (Gibco). Additional cell debris was aspirated off and fresh media added daily until cells reached confluency ...
-
bioRxiv - Bioengineering 2021Quote: ... and subjected to heat- induced antigen retrieval using sodium citrate buffer(10 mM, pH 6, ThermoFisher, Waltham, MA) at >80°C ...
-
bioRxiv - Neuroscience 2020Quote: Neocortex from C57Bl/6 pups (postnatal day 1-3) was dissected in Hibernate-A medium (#A1247501, Gibco, USA), digested with papain (#LS003124 ...
-
bioRxiv - Microbiology 2021Quote: HEK293T cells seeded in 6 well plates were transfected with the indicated plasmid combinations using Lipofectamine reagent (INVITROGEN). At 24 hours post transfection ...
-
bioRxiv - Microbiology 2022Quote: ... the cells were rinsed in PBS and the nucleus stained with 4′,6-diamidino-2-phenylindole (DAPI, ThermoFisher). Coverslips were then mounted and imaged with the Zeiss Laser Scanning 710 confocal microscope images were processed using Zeiss Zen 2011 software.
-
bioRxiv - Molecular Biology 2019Quote: ... Total RNA was extracted from 6 pairs of OSCC and normal buccal pouch samples using TRIzol (Invitrogen, USA) according to the protocol provided by the manufacturer ...
-
bioRxiv - Neuroscience 2019Quote: ... they were transfected with plasmid expression constructs in 6-well tissue culture plates (Thermo Fisher Scientific, Burlington, ON) using Lipofectamine 3000 transfection reagent (Life Technologies ...
-
bioRxiv - Cancer Biology 2019Quote: ... 240 μl of the lysate was digested with 6 μl of RNase I (AM2294, 100 U/μl Invitrogen) at room temperature with rotation ...
-
bioRxiv - Neuroscience 2019Quote: Neurons were grown on 6 cm dishes and treated with or without 10 μM chloroquine (Fisher Scientific, ICN19391980) in maintenance medium for 16 h ...
-
bioRxiv - Cell Biology 2020Quote: ... at 37°C and 5% CO2 with 6 mL HDF medium consisting of 1x Advanced DMEM (Gibco™), 1x Glutamax™ (Gibco™) ...
-
bioRxiv - Genomics 2020Quote: ... the cells were split 1:6 with Accutase (StemPro® Accutase® Cell Dissociation Reagent, Gibco™ A1110501), onto matrigel substrate in the presence of 10 μM of rock inhibitor (Y-27632 dihydrochloride ...
-
bioRxiv - Bioengineering 2020Quote: ... Stained sections were mounted in Slow Fade GOLD with 4’,6-diamidino-2-phenylindole (DAPI) (Molecular Probes, OR) and observed using a Nikon T300 Inverted Fluorescent microscope (Nikon Corp. ...
-
bioRxiv - Systems Biology 2021Quote: ... splenocytes were obtained from naïve C57BL/6 mice and stained with CellTrace Pacific Blue Cell Proliferation Kit (ThermoFisher). Stained splenocytes (2.5 × 105 ...
-
bioRxiv - Molecular Biology 2020Quote: ... and size selected for 130-200 nt fragments on a pre-casted 6% polyacrylamide Novex TBE gel (Invitrogen). Gel extraction was done using Gel breaker tubes (IST Engineering Inc ...
-
bioRxiv - Neuroscience 2021Quote: ... Brain slices were incubated in 4’,6-diamidino-2-phenylindole (DAPI, Acros Organics–Thermo Fisher Scientific, Geel, Belgium) and mounted on microscope slides in mounting medium (Vectashield ...
-
bioRxiv - Cancer Biology 2020Quote: ... mitochondria were purified from 4-6 × 107 cells using mitochondria isolation kit for cultured cells (Thermo Fisher Scientific) according to manufacturer instructions ...
-
bioRxiv - Cancer Biology 2021Quote: ... qRT-PCR reactions were performed in triplicate using QuantStudio 6 Flex Real-Time fast PCR System (Applied Biosystems) and 2-ΔΔCt values were used to calculate the relative expression level of the target genes compared to controls ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... PDMS coverslips were manually added to each well of 96/6 well plates (ThermoFisher Scientific, Waltham, MA USA) and each plate was subsequently sterilized ...
-
bioRxiv - Developmental Biology 2019Quote: ... 6 mM β-mercaptoethanol and 0.5% NP-40) supplemented with 75 units/ml RNAse inhibitor (Thermo Fisher Scientific) and 1X mammalian protease inhibitor cocktail (Roche ...
-
bioRxiv - Pharmacology and Toxicology 2021Quote: ... and 6 μg of pcDNA-SARS-CoV-2-S(ΔC19) in 1 ml of Opti-MEM medium (ThermoFisher). Then ...
-
bioRxiv - Microbiology 2019Quote: ... The slides were mounted using ProLong diamond antifade mountant with DAPI (4′,6′-diamidino-2-phenylindole) (Invitrogen P36962).
-
bioRxiv - Synthetic Biology 2019Quote: ... 6 mM L-glutamine (2 mM from Gibco 31600-091 and 4 mM from additional Gibco 25030-081), penicillin (100 U/µL) ...
-
bioRxiv - Systems Biology 2020Quote: ... diluted to 100mM HEPES and 4M GnHCl and pH adjusted pH6.5-7.5) prior to 6-plex TMT labelling as per manufacturer’s instructions (Sixplex TMT, Cat# 90061, ThermoFisher Scientific). Equal ratios of TMT-labelled samples were pooled and methanol/chloroform precipitated ...
-
bioRxiv - Bioengineering 2021Quote: The 6 most concentrated fractions were concentrated and desalted with 6mL 3MWCO protein concentrator columns (Pierce, Thermo Fisher) at 4C 4000xg 4 hrs and stored at 4C ...
-
bioRxiv - Cancer Biology 2020Quote: ... Cells were transfected with 6 μg plasmid DNA per 106 cells using Neon transfection system (Thermo Fisher Scientific) using parameters 1400 V ...
-
bioRxiv - Immunology 2020Quote: ... Cells were then stained with 1 μg/mL 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes/Thermo Fisher) and analysed on a FACSVERSE (BD Biosciences) ...
-
bioRxiv - Immunology 2020Quote: ... Cells were then stained with 1 μg/mL 4′,6-diamidino-2-phenylindole (DAPI; Molecular Probes/Thermo Fisher) and analysed on a FACSVERSE (BD Biosciences) ...
-
bioRxiv - Developmental Biology 2019Quote: ... day 4- and day 6-control and mutant ESC-derived neurospheres were collected and treated with Trizol (Invitrogen). RNAs were quantified by a Nanodrop instrument ...
-
bioRxiv - Molecular Biology 2020Quote: mESCs were dissociated with Accutase™ and seeded in Nunclon Sphera 6-well plates (Thermo Fisher Scientific, # 174932) at a cell density of 11,000 cells / well in Serum + LIF medium ...
-
bioRxiv - Cell Biology 2019Quote: ... RFL-6 cell were purchased from the American Type Culture Collection (ATCC) Ham’s F-12K medium (Thermo Fisher) supplemented with 20% FBS ...
-
bioRxiv - Neuroscience 2019Quote: ... Cells were passaged 1:2-1:6 every 2-5 days by being rinsed once with DPBS (Gibco) and dissociated using 0.5 mM EDTA (75 μl/cm2 ...
-
bioRxiv - Biochemistry 2019Quote: ... or mismatch plasmids (1.5 μg per well in 6-well plate) using Lipofectamine 3000 Reagent (Thermo Fisher Scientific) following the manufacturer’s protocol.
-
bioRxiv - Genomics 2021Quote: ... hiPSC-IMR90-1 cells were cultured on a Matrigel-coated 6-well plate with Essential 8 medium (Gibco). The medium was changed daily ...
-
bioRxiv - Genetics 2021Quote: ... The relative mtDNA copy number was assessed using QuantStudio 6 Flex Real-Time PCR system (Thermo Fisher Scientific). The mtDNA and the nuclear-encoded housekeeping gene 18S were amplified using primer sequences ...
-
bioRxiv - Genetics 2020Quote: ... Cells were passaged 1:2-1:6 every 2-4 days by being rinsed once with DPBS (Gibco) and dissociated using 0.5 mM EDTA (75 µl/cm2 ...
-
bioRxiv - Immunology 2021Quote: ... were cocultured at 1:1 ratio for 6 days with anti-CD3/CD28–coated beads (Dynabeads, Life Technologies) at 1 bead ...
-
bioRxiv - Physiology 2020Quote: ... DAPI (4’,6-Diamidino-2-Phenylindole Dihydrochloride; 1:2000 diluted in PBTD; Thermo Fisher Scientific, Stockholm, Sweden; 62248) was added for 3 minutes followed by washing in PBTD for 25 minutes and mounting with ProLong gold antifade reagent (Thermo Fisher Scientific ...
-
bioRxiv - Neuroscience 2019Quote: ... the medium in the 6 well plates was replaced with pure OMEM without phenol (#11058-21, ThermoFisher Scientific) by adding 1ml per well ...
-
bioRxiv - Cancer Biology 2021Quote: Cells were stimulated for a total of 6 hours with human T-activator anti-CD3/CD28 Dynabeads (ThermoFisher) stimulation with GolgiStop solution being added for a total of 5 hours block intra-cellular protein transport (BD Bioscience) ...
-
bioRxiv - Physiology 2021Quote: ... 6-10 microliters of a mixture of green fluorescent particles (Thermo Scientific, 1.05 g/cm3, fluorescence 589 nm) were injected using a 2.5 μm Hamilton glass syringe with a pulled borosilicate capillary tube (Fig.2A) ...
-
bioRxiv - Developmental Biology 2021Quote: ... After 30 min incubation with 100 ng/mL 4’,6-diamidino-2-phenylindole (DAPI; Thermo Fisher Scientific D1306), specimens were washed three times with PBS−/− ...
-
bioRxiv - Cell Biology 2021Quote: ... supplemented with 1% penicillin–streptomycin in 6-well plates coated with truncated recombinant human vitronectin (Thermo Fisher Scientific). The iPSC line was previously assessed for pluripotency and normal karyotype.33 For differentiation ...