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Citations for Thermo Fisher :
4501 - 4550 of 6549 citations for 6 Chloro N methoxy N methyl nicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2024Quote: Total RNA was extracted from 30 ovaries from 3-6 day-old flies using TRIzol (Thermo Fisher Scientific) in three replicates ...
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bioRxiv - Cell Biology 2024Quote: ... we used 1-(4-Trimethylammoniumphenyl)-6-Phenyl-1,3,5-Hexatriene p-Toluenesulfonate (TMA-DPH; Thermofisher Scientific Cat. No. T204). Yeast cells were stained with 0.5µM TMA-DPH ...
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bioRxiv - Physiology 2024Quote: ... 6 rat samples (ExpA, B and C) were placed directly in Leibovitz’s L-15 medium (Gibco 11415-064) to develop organoids immediately ...
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bioRxiv - Cell Biology 2024Quote: ... RealQ Plus 2X Master Mix (Amplicon) was used for qPCR amplification on a QuantStudio 6 Flex (Applied Biosystems). For Western Blot ...
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bioRxiv - Microbiology 2024Quote: ... Propidium iodide (PI) and the pH sensitive 2’,7’-Bis-(2-Carboxyethyl)-5-(and-6)-Carboxyfluorescein (BCECF) (Invitrogen) were added to these at a final concentration of 100µM and 10µM respectively ...
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bioRxiv - Neuroscience 2024Quote: ... Nuclear staining was performed using 4′,6′-diamidino-2-phenylindole dihydrochloride (DAPI; 2 ng ml−1; Molecular Probes). Sections were cover-slipped using ProLong Glass mounting agent (ThermoFisher) ...
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bioRxiv - Microbiology 2024Quote: ... RNA yield and integrity was verified using a NanoDrop spectrophotometer and a Novex 6% TBE-urea gel (Invitrogen), respectively.
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bioRxiv - Cancer Biology 2024Quote: ... cells were transfected with 6 nM siRNA mixed with OptiMEM reduced serum medium (Thermo Fisher Scientific, Cat# 31985062) using Lipofectamine RNAiMAX transfection reagent (Invitrogen ...
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bioRxiv - Microbiology 2024Quote: ... Then, 4′,6-diamidino-2-phenylindole (DAPI, Solabio) and Alexa Fluor 488 Conjugate Concanavalin-A (Invitrogen, CA, USA) were added to each slide and incubated for 5 min at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... cells-were transfected in 6-cm plates 24 hours after second siRNA transfection with Lipofectamine 2000 (ThermoFisher Scientific) and 2 μg of pCMV6M-Pak1-WT (Addgene ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were seeded in 6-well plates directly with the siRNA/transfection mix: 3µl of LipoRNAiMax (Life Technologies), 500µL of OptiMEM (Life Technologies) ...
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bioRxiv - Biochemistry 2024Quote: ... 10 µL of this mix was added into wells of a 12-well 6% DNA retardation gel (Invitrogen) that was pre-running for at least 30 minutes in 0.5X TBE at 150V ...
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bioRxiv - Microbiology 2024Quote: ... Beads were washed 6 times with 1 mL each of ChIP buffer on a magnetic tube rack (Invitrogen). After removing the final wash ...
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bioRxiv - Developmental Biology 2024Quote: ... for quantitative reverse transcription PCR analysis on the StepOne/QuantStudio 6 Flex Real Time PCR Systems (Applied Biosystems). The housekeeping gene GAPDH was used as an endogenous control ...
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bioRxiv - Cell Biology 2024Quote: We seeded HEK293T cells at 2.5 × 10^6 cells per 15 cm plate in DMEM (Thermo Fisher 11965118), enriched with 10% FBS (Atlanta Biologicals S11150H) ...
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bioRxiv - Evolutionary Biology 2024Quote: ... Resulting libraries were size selected for 145-160bp fragments using Novex 6% TBE gels (Invitrogen, Cat. No. EC6265BOX) to retain only the small RNA inserts ...
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bioRxiv - Cell Biology 2024Quote: ... Z-stack images were taking on day 6-7 after seeding using EVOS™ 7000 Imaging System (Invitrogen). and used for quantification of organoids.
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bioRxiv - Biochemistry 2024Quote: ... 6 second blot time and 10 second wait time before plunge freezing using a vitrobot MarkIV (ThermoFisher Scientific) set at 100% humidity and 4 °C ...
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bioRxiv - Bioengineering 2024Quote: ... RC Dialysis tubing (3.5 kDa or 8 kDa molecular weight cut-off, Spectrum Spectra/Por 6, Fisher Scientific), HCl (2M ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were subcultured at a ratio of 1:10 every 5–6 days using 0.5 mM EDTA (Invitrogen) to detach clumped cells.
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bioRxiv - Cancer Biology 2024Quote: ... and qPCR was performed using the QuantStudio 6 Flex Real-Time PCR System with SYBR green (ThermoFisher Scientific). Expression was normalized to TBP ...
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STAT3 protects HSCs from intrinsic interferon signaling and loss of long-term blood-forming activitybioRxiv - Immunology 2023Quote: ... mounted with 10 µL of ProLong Diamond with DAPI (4’,6-diamidino-2-phenylindole; Molecular Probes, Eugene, OR), and incubated for 24-48 hours prior to analysis ...
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bioRxiv - Microbiology 2023Quote: ... and counterstained with 4′,6-diamidin-2-phenylindole (DAPI, 4 μg mL-1, Thermo Fisher Scientific, MA, USA). From the 18 biofilms from each year ...
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bioRxiv - Plant Biology 2023Quote: ... and qPCR was performed using the indicated oligos (Table S4) (QuantStudio 6 Pro PCR system; Thermo Fisher Scientific) using Power SYBR Green Master Mix (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2023Quote: ... droplets were put in 6-well Ultra Low Attachment Plates (Thermo Fisher Scientific P6 : REF 150239 “untreated Nunc”) in Expansion Medium without vitamin A (for 50 mL ...
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bioRxiv - Neuroscience 2023Quote: ... RT-qPCR was performed by running the following thermocycler program on a QuantStudio 6 Flex system (Applied Biosystems): 50C for 15 min ...
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bioRxiv - Developmental Biology 2023Quote: ... Gut orientation was analyzed by treating MZ myo1dpt31cand wildtype larvae with 0.3 μg/ml PED-6 (ThermoFisher #D23739) for 3 hours at 6 days post fertilization as described88 ...
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bioRxiv - Molecular Biology 2023Quote: ... Real-time PCR was performed using the QuantStudio 6 Flex Real-Time PCR System (Thermo Fisher Scientific, USA).
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bioRxiv - Pathology 2023Quote: ... Culture identity was confirmed using species-specific qPCR assays on the Quantstudio 6 platform (Applied Biosystems, MA USA); see Table S1.
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bioRxiv - Molecular Biology 2022Quote: ... HeLa were prepared in 6-well plates 24 hours before the transfection with 4.5.105 cells/well in RPMI medium (Gibco-Invitrogen) supplemented with 10% fetal calf serum (Gibco-Invitrogen) ...
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bioRxiv - Molecular Biology 2023Quote: ... the cells were transfected as follows: 2 μg eGFP-SHLD3 constructs and 6 μl Lipofectamine 2000 (Thermo Scientific) were incubated in Opti-MEM for 20 min at room temperature before being added to the cells whose media had been changed to McCoy’s with 10% FBS and no antibiotics ...
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bioRxiv - Cell Biology 2023Quote: ... 800,000 cells were seeded into a 6-well plate (NuncMicroWell Plates with Nunclon; ThermoFisher Scientific, Waltham, MA, USA). At day 0 the transfection was done ...
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bioRxiv - Cell Biology 2023Quote: ... The resulting pellet was resuspended and differentiated for 6 days in culture media containing: DMEMF12 (Gibco, #11330-032) supplemented with 20% FBS (Gibco ...
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bioRxiv - Cell Biology 2023Quote: ... Nuclei were stained by incubation with 0.5 – 1 µg/mL 4’,6-diamidino-2-phenylindole (DAPI, D1306, Invitrogen) in PBS for 10 min at RT ...
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bioRxiv - Immunology 2023Quote: ... when floating cells were collected and plated into new 6 cm dishes with Maturation Media (RPMI (Gibco, 61870036), 10% FBS ...
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bioRxiv - Microbiology 2023Quote: ... thetaiotaomicron using a Applied Biosystems QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems, Foster City, CA, USA), primer sets BT2156-BT2160 ...
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bioRxiv - Developmental Biology 2023Quote: ... were isolated from embryos incubated for between 6 (E6.0) and 8 (E8.0) days and maintained in chilled Leibovitz’s L-15 media (GIBCO, Invitrogen). Papillae were dissected as described previously 65 and cultured nerve-side-down on Millicell cell culture inserts (Millipore ®) ...
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bioRxiv - Bioengineering 2023Quote: ... Droplets of a 5 μL cell-Matrigel mixture were manually deposited onto a high-performance #1.5 glass-bottom 6-well plate (0.170±0.005 mm) (Fisher Scientific). The datasets were separated into non-treatment and treatment groups ...
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bioRxiv - Neuroscience 2023Quote: ... 6-well plate (500x103 cells/well) in Neural Expansion Medium (NEM: 49% Neurobasal, 49% Advanced DMEM (Thermo Scientific), 2% Neural Induction Supplement ...
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bioRxiv - Neuroscience 2023Quote: ... EBs were seeded on one well of a 6-well plate coated with poly-Ornithine/Laminin (Thermo Scientific). Medium was changed every other day ...
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bioRxiv - Neuroscience 2023Quote: ... cells were incubated in Cellvis 6 Well glass bottom plates with high performance #1.5 cover glass (Fisher Scientific). For control wells to be pre-treated with cathepsin inhibitors ...
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bioRxiv - Neuroscience 2023Quote: ... The injected individuals were kept at 18°C for 5 to 8 days in 6-well-plates (Nunc multidish no ...
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bioRxiv - Neuroscience 2023Quote: ... transfections were performed directly after splitting the cells in 6-well plates using Lipofectamine 2000 Reagent (Invitrogen, 11668019) following the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Grids were blotted for 4-6 seconds with blotforce of 15 on a Vitrobot Mark IV Vitrobot (ThermoFisher) and directly plunged into liquid-nitrogen-cooled liquid ethane.
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bioRxiv - Cancer Biology 2023Quote: ... This insert creates a clean cell-free gap in a 6-or 12-well plate (Thermo Scientific, 150239). The assay was performed according to the manufacturer’s protocol (ibidi) ...
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bioRxiv - Cancer Biology 2023Quote: ... B6.Cas9 keratinocytes(see above) were plated in 6-well plate (Thermo Scientific Nunclon TM Delta Surface; 140675) at 1.5 × 105 cells per well and infected with 100-300μl of low-titer virus in the presence of infection mix (1/10 dilution of polybrene [10 mg ml−1 Sigma ...
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bioRxiv - Evolutionary Biology 2023Quote: ... approximately 10 female and 6 male flies were placed into a flask (Fisher Scientific, Cat. No.:AS-355) of food and maintained at 25°C for one week to allow for mating and egg laying ...
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bioRxiv - Molecular Biology 2023Quote: ... U2OS cells were infected (24 h) in viral mixture (1.5 mL DMEM, 1.5 mL viral harvest, 6 µL polybrene, Invitrogen) and DMEM with 7.5 µg/mL blasticidin (Sigma ...
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bioRxiv - Microbiology 2023Quote: ... Plates were then washed 6× and 50 µl of 1-Step™ 3,3’,5,5’-tetramethylbenzidine (TMB; Thermo Fisher) substrate was added to each well and incubated for 3 min ...
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bioRxiv - Genomics 2023Quote: ... All nuclei were pooled and stained with DAPI (4′,6-diamidino-2-phenylindole, 3 uM final) (Invitrogen, D1306). Using a FACSAria III cell sorter (BD Biosciences) ...