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Citations for Thermo Fisher :
4351 - 4400 of 6549 citations for 6 Chloro N methoxy N methyl nicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2019Quote: ... Nuclei were counterstained with 4’,6-diamidino-2-phenylindole (DAPI, 1:5000 in PBS) (Molecular Probes, Eugene, OR). The TUNEL stain signal was observed under an FV300 confocal microscope (Olympus Optical Company ...
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bioRxiv - Genetics 2019Quote: ... 0.1mM 6-Caboxyfluorescein-TEG azide (FF 6110; Berry & Associates) or Alexa Fluor® 594 Azide (A10270; Invitrogen™), and 2mM copper (II ...
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bioRxiv - Molecular Biology 2019Quote: ... Aliquots were solubilized in 1 mL 8M urea lysis buffer: 8M Urea (Invitrogen, CA; CAS# 57-13-6), 100mM NaCl(Fisher Scientific ...
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bioRxiv - Biochemistry 2020Quote: ... and transfected the cells with 2000 ng of plasmid DNA and 6 μl of Lipofectamine 2000 reagent (Invitrogen) according to the manufacturer’s protocols ...
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bioRxiv - Cell Biology 2019Quote: ... The q-RT-PCR reactions were run on the QuantStudio 6 Flex Real-Time PCR system (Applied Biosystems). qPCR results were analyzed using the ΔΔCt method relative to the mean of housekeeping genes (Rps12 ...
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bioRxiv - Microbiology 2021Quote: ... 20-40 mg of total RNA was incubated with 6 units of TURBOTM-DNase (Thermo Fisher Scientific AM2238), 1X TURBO™DNase buffer ...
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bioRxiv - Neuroscience 2020Quote: ... the tissues were counterstained with DAPI (4’,6-diamidino-2-phenylindole) and mounted with Prolong Gold (Life Technologies). Fluorescence was detected using an epifluorescence microscope (Nikon Eclipse NiE).
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bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-6 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
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bioRxiv - Bioengineering 2020Quote: ... Cells migrating through the porous membrane are identified by staining with 4′,6-diamidino-2-phenylindole (DAPI; Invitrogen), counting five representative fields per insert ...
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bioRxiv - Bioengineering 2020Quote: ... The vector was transfected to 6×105 cells of HEK293T cells using Opti-MEM (Thermo Fisher Scientific, MA) and a Lipofectamine reagent (Thermo Fisher Scientific ...
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bioRxiv - Microbiology 2021Quote: The 6-O-desulfated Arixtra (30 µM) was analyzed on a Thermo Orbitrap Fusion Tribrid (Thermo Fisher Scientific) coupled with an Ultimate 3000 Nano LC system (Dionex ...
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bioRxiv - Biophysics 2021Quote: ... Desiccated samples were subjected to 5-6 rounds of desiccation and rehydration (1-hour speedvac desiccation [Thermo Fisher Scientific] followed by resuspension in water) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were resuspended in MACS buffer containing 2 µg/mL 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies). A BD FACSAria III Cell Sorter (BD Biosciences ...
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bioRxiv - Neuroscience 2021Quote: ... The slides were washed in TNT and stained with 4’,6-diamidino-2-phenylindole (DAPI; Invitrogen, catalog # D3571), washed again ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were mounted on slide using Antifade mounting media with DAPI (4-6-diamidino-2-phenylindole; ThermoFisher). After sealing the coverslips with clear nail polish ...
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bioRxiv - Biophysics 2021Quote: ... Then the enzyme-inhibitor mixture was placed in a 6–8 kDa MWCO dialysis membrane (Fisher Scientific, Canada) and dialyzed against 2 L of 50 mM Tris-HCl ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... Reverse 5′-CGA AGG TGT GAC TTC CAT G-3′) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). Standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Cancer Biology 2020Quote: ... Nuclei were stained with 4’,6-diamidino-2-phenylindole (DAPI) in ProLong Diamond Antifade Mountant (Thermo Fisher # P36971). Images were captured using a Nikon Eclipse Ti2 microscope ...
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bioRxiv - Biochemistry 2021Quote: ... and then diluted into fresh YPD for 3-6 hours before inoculating into 96-well plates (Thermo Scientific) at a starting OD600 between 0.04 to 0.1 ...
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bioRxiv - Cancer Biology 2020Quote: Cells for Western blot analysis were treated in 6 well plates (Thermo Fisher Scientific, Cat# 07-200-83) or 10 cm dishes (USA Scientific ...
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bioRxiv - Microbiology 2021Quote: ... 2×104 iBMDMs (6-well plates) were transfected in suspension with 20 nM siRNA using Lipofectamine RNAiMAX (Invitrogen) in 200 μ Opti-MEM I (ThermoFisher).AllStars negative-control siRNA (Qiagen ...
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bioRxiv - Microbiology 2021Quote: ... L454W/E455G and S262R) and mCherry (6:4:1 mixtures; 2.2 μg/well) using Lipofectamine® 2000 (Invitrogen), according to the manufacturer’s recommendations ...
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bioRxiv - Microbiology 2021Quote: ... Cover slips were mounted using Prolong Gold Antifade reagent containing 4’,6-diamidino-2-phenylindole (DAPI; Life Technologies) and incubated overnight at 37°C ...
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bioRxiv - Microbiology 2021Quote: HEK293T cells seeded in 6 well plates were transfected with the indicated plasmid combinations using Lipofectamine reagent (INVITROGEN). At 24 hours post transfection ...
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bioRxiv - Microbiology 2021Quote: ... The qRT-PCR was performed on an ABI QuantStudio™ 6 Quantitative Real-Time PCR System (Applied Biosystems) under the following conditions ...
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bioRxiv - Microbiology 2020Quote: Huh7 cells were plated in 6 well plates (2,5×105 cells/well) in DMEM (Thermo Fisher Scientific, US) supplemented with 10% heat-inactivated FBS (Thermo Fisher ...
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bioRxiv - Microbiology 2020Quote: HEK293T cells were cultured in 6-well plates and infected with DNAse I (Thermo Fisher Scientific, Schwerte, Germany) treated viruses for 12 hours ...
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bioRxiv - Microbiology 2019Quote: ... Mice were euthanized by CO2 asphyxiation at 6 h and lungs placed in 1 ml TRIzol (Thermo Fisher). RNA was then extracted using acid-phenol-chloroform-isoamyl alcohol (125:21:1 ...
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bioRxiv - Microbiology 2020Quote: ... The cellular nucleus was stained in blue using 4’,6-diamidino-2-phenylindole (DAPI, Fisher Scientific, Illkirch, France). Uninfected DPSCs were used as negative controls.
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bioRxiv - Cell Biology 2019Quote: Cells were seeded in 6-well plates 24 hours prior to transfection and transfected using Lipofectamine 2000 (Invitrogen). Transfection efficiency was visually observed using fluorescent microscopy and quantified using flow cytometry ...
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bioRxiv - Cell Biology 2021Quote: ... Nucleus staining reagent 4’,6-diamidini-2-phenylindole (DAPI, #D1306) and Hoechst33342 (#H21492) were purchased from Thermo Fisher Scienfitic.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were seeded in 6-well plates in 1 ml of RPMI 1640 medium (Gibco, Amarillo, TX, USA) supplemented with 10% fetal bovine serum and 0.2% primocin (Invivogen ...
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bioRxiv - Cell Biology 2021Quote: ... Cas9-expressing cells were then selected with blasticidin antibiotic at 50 μg/mL for 6 days (Life technologies). Cas9-expressing hCMEC/D3 cells were each transduced separately by lentiviral particles expressing sgRNAs and selected by puromycin resistance at 10 μg/mL for 6 days (Life technologies) ...
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bioRxiv - Cell Biology 2021Quote: ... marrow MSC-L (100-300 cells) were sorted directly into 6-well plates containing 1.5 ml of Dulbecco’s Modified Eagle Medium (Gibco) supplemented with 20% FBS (CellGro) ...
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bioRxiv - Cell Biology 2021Quote: ... Coverslips were mounted using prolonged diamond anti-fade mounting media containing DAPI (4, 6-diamidino-2-phenylindole; Invitrogen) and imaging was done on Nikon laser scanning confocal microscope Plan Apo VC 100X (oil ...
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bioRxiv - Cell Biology 2021Quote: ... as per manufacturer’s protocol in 6 biological replicates and quantified using a Nanodrop 1000 spectrophotometer (Thermo Fisher Scientific). Following treatment with 1U of DNase I (amplification grade ...
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bioRxiv - Cell Biology 2022Quote: 6-dpf zebrafish larvae were gavaged with Alexa Fluor 488-tagged dextran 10,000MW (fDex) solution (Molecular Probes, USA) or purified mCherry solution (prepared in the laboratory ...
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bioRxiv - Bioengineering 2022Quote: ... 4-Chlorobenzenesulfonate Salt (DID) and 4′,6-diamidino-2-phenylindole (DAPI) were purchased from Invitrogen (Carlsbad, CA, USA). Ammonium bicarbonate ...
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bioRxiv - Cell Biology 2022Quote: ... An aliquot of 6 μL was collected for protein quantification using the BCA Protein Assay (ThermoFisher Scientific 23227) and the remaining clarified lysates were kept at −20°C until SDS-PAGE analysis.
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bioRxiv - Systems Biology 2022Quote: ... Droplets of 5 μL cell-Matrigel mixture were manually deposited onto a high performance #1.5 glass bottom 6-well plate (0.170±0.005 mm) (Fisher Scientific) (Fig ...
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bioRxiv - Microbiology 2022Quote: ... were cultivated in culture treated plastics (T-75s; T-25s and 6 well plates) presence of Dulbecco’s modified Eagle’s medium (DMEM-Gibco) supplemented with 10% foetal bovine serum (SIGMA) ...
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bioRxiv - Biochemistry 2022Quote: ... and blotted for 4 to 6 seconds at 4°C and 100% humidity using the Vitrobot system (ThermoFisher), before plunging immediately into liquid ethane for vitrification ...
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bioRxiv - Cell Biology 2022Quote: ... HeLa cells were cultured to ∼60% confluency in 6-well plates and transfected with Lipofectamine 3000 (Invitrogen L3000) following directions provided by the manufacturer ...
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bioRxiv - Cell Biology 2022Quote: ... samples were incubated for 30 min at 37°C with the secondary antibody diluted in PBS/3% BSA/0.05% Tween 20 (see antibodies section) and 4’,6-diamidino-2-phenylindole (DAPI 0.125 mg/ml, Invitrogen) in PBS/3% BSA/0.05% Tween 20 ...
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bioRxiv - Neuroscience 2022Quote: ... the cerebral cortexes from 2-3 P3-P5 C57BL/6 mice were collected in ice cold HBSS (Invitrogen), the tissue was washed three times with HBSS and digested with 0.04% trypsin (Sigma ...
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bioRxiv - Biochemistry 2022Quote: ... 5 × 105 landing pad cells expressing hACE2 with mNG21-10 were seeded in 6-well plates (Fisher Scientific). The cells were then then incubated at 37 °C with 5% CO2 for 15 mins to allow seeding ...
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bioRxiv - Neuroscience 2022Quote: ... Tax ID 10090) protein database (2017-6-7) using Proteome Discoverer (PD) 2.2 (Version 2.2.0.388; Thermo Fisher Scientific). Label-free quantification was also performed with PD 2.2 using precursor ions quantifier nodes ...
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bioRxiv - Cell Biology 2022Quote: ... All experiments were performed between passages 4 to 6 where cells were dissociated using TrypLE express (Life technologies), a gentle animal origin-free recombinant enzyme and seeded at a cell density of 5000 cells/cm2.
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bioRxiv - Cell Biology 2022Quote: ... Bone chips were plated in 12-well and 6-well plates in MEM-A medium (GIBCO, 12561-056) supplemented with 10% FBS (Cytiva ...
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bioRxiv - Cancer Biology 2022Quote: ... Samples were separated on 6% 0.5X TBE polyacrylamide gels and analyzed using LightShift® Chemiluminescent EMSA Kit (ThermoFisher).