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Citations for Thermo Fisher :
4101 - 4150 of 6549 citations for 6 Chloro N methoxy N methyl nicotinamide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: Vero-cCD150 cell monolayers in 6-well trays were infected with Fowlpox-T7 in Opti-MEM (Gibco) for 30 mins at 37°C and then spinoculated at room temperature for another 30 mins ...
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bioRxiv - Cancer Biology 2021Quote: ... and after 40 cycles were analyzed on an QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems).
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bioRxiv - Cell Biology 2020Quote: ... 6 or 9 µl of 20 µM siRNA and 4 µl of Lipofectamine RNAi MAX (13778150, Invitrogen) were suspended in 100 µl of water (W4502 ...
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bioRxiv - Cell Biology 2020Quote: ... then stained for one minute with 30 ng/mL 4’,6-diamidino-2-phenylindole (DAPI, Invitrogen, D1306) in 1x PHEM ...
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bioRxiv - Cell Biology 2020Quote: ... cells were transitioned to feeder-free culture in Matrigel-coated 6-well plates (Fisher Scientific, Pittsburgh, PA).
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bioRxiv - Cell Biology 2020Quote: ... a 1:1000 dilution of DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride, Molecular Probes; D1306, Molecular Probes)/PBS solution or 5 mg/ml Hoescht (Invitrogen)/PBS solution was carried out for 15 min at RT ...
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bioRxiv - Cell Biology 2020Quote: ... a 1:1000 dilution of DAPI (4’,6-diamidino-2-phenylindole, dihydrochloride, Molecular Probes; D1306, Molecular Probes)/PBS solution or 5 mg/ml Hoescht (Invitrogen)/PBS solution was carried out for 15 min at RT ...
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bioRxiv - Developmental Biology 2020Quote: ... or mouse mesenchymal stem cells C3H-Tom-Gfp and C3H-Tom-Egr1 were plated on 6-well plates at a density of 33.000 cells/well in Dulbecco’s Modified Eagle’s Medium (DMEM, Invitrogen) supplemented with 10% foetal bovine serum (FBS ...
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bioRxiv - Developmental Biology 2021Quote: ... Tissue nuclei were visualized with nuclear stain 4′,6-diamidino-2-phenylindole (DAPI, 62247; Thermo Fisher Scientific). Coverslips were mounted using Prolong Gold (P36934 ...
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bioRxiv - Neuroscience 2021Quote: ... Levels of TNFα and IL-6 in culture supernatants were measured using mouse ELISA kits (Thermo Scientific).
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bioRxiv - Molecular Biology 2020Quote: ... The transfection mixtures were replaced at 6 h post-transfection by 293 SFM II expression medium (Gibco), supplemented with sodium bicarbonate (3.7 g/L) ...
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bioRxiv - Molecular Biology 2021Quote: 6 μg total oocyte RNA from pooled injected oocytes was treated with 10 U RNase H (Ambion) and 500 ng oligo(dT ...
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bioRxiv - Molecular Biology 2021Quote: ... All qPCR runs were performed using QuantStudio-6/ ViiA7/ 7900HT Fast Real-Time PCR System (Applied Biosystems) on 96-well or 384-well plates ...
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bioRxiv - Molecular Biology 2020Quote: ... The transfection mixtures were replaced at 6 h post-transfection by 293 SFM II expression medium (Gibco), supplemented with sodium bicarbonate (3.7 g/L) ...
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Prom1 and Notch regulate ciliary length and dynamics in multiciliated cells of the airway epitheliumbioRxiv - Cell Biology 2022Quote: ... 4′,6-diamidino-2-phenylindole (DAPI) was used to stain intracellular DNA (NucBlue, Thermo Fisher, Waltham, MA). Alexa Fluor 647 Phalloidin (Thermo Fisher ...
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bioRxiv - Genomics 2020Quote: ... Sections were stained with DAPI (4′,6′-diamidino-2-phenylindole) and mounted in Prolong Gold (Life Technologies). Images were obtained by confocal microscopy (Nikon C2+ Eclipse ...
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bioRxiv - Biophysics 2019Quote: ... 106 cells plated per well of a 6-well plate were transfected with 150 pmol siRNAs (Ambion: Negative Control #AM4611 ...
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bioRxiv - Biophysics 2019Quote: Primary mouse cortical neurons (MCN) from C57BL/6 embryonic day-17 mice were obtained from Thermo Fisher Scientific (cat ...
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bioRxiv - Biophysics 2019Quote: ... and spun at 31,920 g for 10 minutes in a Sorvall RC 6+ (Thermo Scientific, Waltham MA) centrifuge using an F18-12×50 rotor (Thermo Scientific ...
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bioRxiv - Neuroscience 2019Quote: ... Tissue was washed in PBS before counterstaining with 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) and mounting ...
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bioRxiv - Animal Behavior and Cognition 2019Quote: ... 6 μg of mRNA was reverse-transcribed with RevertAid First-Strand cDNA Synthesis Kit (Thermo Scientific, USA) using a thermal cycler (T100TM Thermal Cycler ...
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bioRxiv - Cell Biology 2019Quote: Laurdan (6-dodecanoyl-2-dimethylaminonaphthalene; product D250) and di-4-ANEPPDHQ (product D36802) were purchased from ThermoFisher. Methyl-β-cyclodextrin (product C4555 ...
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bioRxiv - Biochemistry 2019Quote: ... Plates were sealed using optically clear films and transferred to a QuantStudio 6 qPCR instrument (Applied Biosystems). The fluorescence intensity was measured during a temperature gradient from 25 to 95 °C at a constant rate of 0.05 °C/s ...
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Therapy-induced lipid uptake and remodeling underpin ferroptosis hypersensitivity in prostate cancerbioRxiv - Cancer Biology 2020Quote: ... and Nuclear DNA was counterstained with 1 µg/ml 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher). Alternatively ...
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bioRxiv - Developmental Biology 2019Quote: Total RNA from whole retinas was extracted using TRIzol (6 retinas from 3 fish per sample) (Invitrogen). RNA was quantified using Nanodrop spectrophotometer (Thermo Fisher Scientific) ...
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bioRxiv - Microbiology 2019Quote: All samples were counterstained with DAPI (4’,6-diamidino-2-phenylindole; Invitrogen, Fisher Scientific AG, Reinach, Switzerland) to visualize nuclei ...
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bioRxiv - Genetics 2019Quote: ... SYBR green real-time PCR cycling conditions using QuantStudio 6 Flex Real-Time PCR system (ThermoFisher Scientific) and amplification efficiency calculation (E=e(−1/slope) ...
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bioRxiv - Cell Biology 2021Quote: ... then stained for one minute with 30 ng/mL 4′,6-diamidino-2-phenylindole (DAPI, Invitrogen D1306) in 1x PHEM ...
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bioRxiv - Cancer Biology 2021Quote: ... 0.5-1×104 cells per well were seeded in 6-well Falcon plates (Fisher Scientific, Cat #087721B) in triplicate ...
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bioRxiv - Neuroscience 2020Quote: ... the slides were incubated in 1μg/ml of 4’,6-diamidino-2-phenylindole (DAPI, Thermo Fisher Scientific) at RT for 10min ...
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bioRxiv - Biophysics 2021Quote: ... Membranes were stained with 1-(4-trimethylammoniumphenyl)-6-phenyl-1,3,5-hexatriene p-toluenesulfonate (TMA-DPH; Molecular Probes) at a final concentration of 100 μM and the cells were then immobilized on a 2% agarose pad made with sporulation buffer covered with a no.1.5 coverslip ...
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bioRxiv - Cancer Biology 2020Quote: ... HCC1937 cells were seeded in a 6 well plate and treated with CYC065 (Cyclacel) or CVT313 (Thermofisher) at the calculated IC5 ...
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bioRxiv - Cell Biology 2021Quote: ... The digested peptides were labelled with 0.2 mg per reaction of 6-plex TMT reagents (ThermoFisher scientific) (126 ...
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bioRxiv - Cell Biology 2021Quote: ... three times for 6 minutes and prepared for mounting using Invitrogen Slow Fade mounting medium (Invitrogen S36917). The cover glass holding the sample (hydrogel plus cells ...
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bioRxiv - Cell Biology 2021Quote: ... Sections were mounted with ProLong Diamond Antifade Mountant with 4′,6-diamidino-2-phenylindole (DAPI; Life Technologies) and imaged using a Leica SP5 DMI (zebrafish sections ...
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bioRxiv - Cell Biology 2021Quote: ... 4′,6-diamidino-2-phenylindole (DAPI) (cat#D-1306) and TRIzolTM (cat#15596018) were purchased from ThermoFisher Scientific (Waltham ...
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bioRxiv - Systems Biology 2020Quote: ... PCR amplification was performed with a QuantStudio™ 6 Flex Real-Time PCR System (Thermo Fisher Scientific) in 20-μl reactions using 1 μl of cDNA (10 ng of total input RNA) ...
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bioRxiv - Biophysics 2020Quote: ... Nuclei were detected by staining with 4’,6-diamidino-2-phenylindole (DAPI, 1:200, ThermoFisher Scientific, USA) for 10 minutes at RT ...
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bioRxiv - Neuroscience 2020Quote: ... The nuclei were stained with 300 nM DAPI (4’,6-diamidino-2-phenylindole; Thermo Fisher Scientific, Slovakia) for 1 min ...
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bioRxiv - Microbiology 2021Quote: ... Reverse 5’-CGA AGG TGT GAC TTC CATG-3’) on a QuantStudio 6 Flex thermocycler (Applied Biosystems). A standard curve was established in parallel using purified SARS-CoV-2 viral RNA.
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bioRxiv - Physiology 2021Quote: ... IL-6 (assay ID: HS00985639_m1) and TNF-α: (assay ID: HS00174128_m1) following manufacture’s protocol (Thermo Fisher Scientific). 18s (assay ID ...
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bioRxiv - Microbiology 2021Quote: ... Desalted peptides were labeled with TMT 6-plex reagents (TMT6plex™ Isobaric Label Reagent Set, Thermo Fisher) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Quantitative real-time PCR was performed on a real-time PCR system (QuantStudio 6 Flex, Applied Biosystems) with Power SYBR Green RNA-to-CT 1-Step Kit (Thermo Fisher Scientific) ...
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bioRxiv - Systems Biology 2020Quote: ... DNA was degraded by adding 6 μl of micrococcal nuclease (0.5 U/ml stock, Thermo Scientific, FEREN0181) and 12 μl of calcium chloride (100 mM stock ...
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bioRxiv - Microbiology 2021Quote: Quantitative polymerase chain reaction (qPCR) was completed on a QuantStudio™ 6 Flex System (Applied Biosystems™) with samples prepared on a 384-well (0.2 mL ...
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bioRxiv - Microbiology 2020Quote: ... Quantitative real-time PCR was performed on a real-time PCR system (QuantStudio 6 Flex, Applied Biosystems) with SYBR Green PCR Master Mix (Life Technologies) ...
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bioRxiv - Microbiology 2019Quote: ... 5 to 6 seeds were pipetted into 120 x 120 mm square petri dishes (Thermo Fisher Scientific) containing half-strength Murashige and Skoog salts (MS) ...
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bioRxiv - Neuroscience 2021Quote: ... or pEGFP-N1-TG2 vector (Furini et al., 2018) using 6 μl of Lipofectamine2000 (Thermo Fisher Scientific) in a total volume of 100 μl per slide (about 1.7×105 neurons) ...
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bioRxiv - Cancer Biology 2020Quote: YUMM1.7 and YUMMER 1.7 cell lines were plated in 6-well dishes and cultured in PBS (Gibco), 1ng/mL ...
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A Polybasic Domain in aPKC Mediates Par6-Dependent Control of Membrane Targeting and Kinase ActivitybioRxiv - Cell Biology 2020Quote: ... and Par-6::Hisx6 were expressed in Expi293F cells via transfection using the ExpiFectamine Transfection Kit (Gibco). Approximately 22.5×107 suspension-adapted Expi293F cells were diluted in Expi293 Expression Medium to the final volume of 76.5 ml and cultured in a 37°C shaker (Eppendorf New Brunswick S41i) ...