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Citations for Thermo Fisher :
4251 - 4300 of 10000+ citations for Aldosterone Chemiluminescent ELISA Kit 5 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2024Quote: ... Each segment of the LACV genome was amplified utilizing primers specified in Table 5 and a Phusion High-Fidelity PCR Master Mix Kit with HF Buffer (Thermo Scientific). All LACV segments were confirmed by a 1% agarose gel and sent to Sanger sequencing using the primers in Table 5 at Genewiz (South Plainfield ...
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bioRxiv - Microbiology 2023Quote: ... cDNA was synthesized from 50 to 200 ηg (5–10 μl) of RNA using the Verso cDNA synthesis kit (Thermo scientific).
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bioRxiv - Plant Biology 2024Quote: ... validations were performed using cDNA synthesised from 5 ng dNase-treated RNA (per qRT-PCR reaction) and a RT Maxima First Strand synthesis kit (Thermo Fisher), following the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2023Quote: 1 × 106 cells/ml were harvested and resuspended in 1× annexin-binding buffer and incubated with 5 μl of Annexin V–488 (Alexa Fluor 488 Annexin V/Dead Cell Apoptosis Kit; Life technologies) for 15 min at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: The gRNAs used in this work were either synthesised by Biomers (synthetic gRNA - without 5’ triphosphate) or in-vitro transcribed using the MEGAshortscript™ T7 Transcription Kit (Invitrogen) (transcribed gRNA - with 5’ triphosphate) ...
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bioRxiv - Molecular Biology 2023Quote: ... Precipitated RNA for 5’ RACE or column purified RNA for qPCR was then treated with DNase using a commercial kit (Turbo DNAse, Ambion, AM2238). Total RNA yield was verified on a 2% agarose gel by visual inspection ...
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bioRxiv - Cancer Biology 2023Quote: Small RNA (<200 bp) was isolated from fibroids and matched myometrium (n= 5) using mirVana miRNA isolation kit (Thermo Fisher Scientific) according to manufacturer’s protocol ...
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bioRxiv - Biochemistry 2023Quote: ... the sample was acidified with formic acid (5% final concentration) and fractionated using high pH reversed-phase peptide fractionation kits (ThermoFisher, 84868) according to Vinogradova and coworkers (Cell ...
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bioRxiv - Plant Biology 2023Quote: Total RNA from 5-day-old whole plants was extracted using the MagMAX™ Plant RNA Isolation kit (Thermo Fisher #A33784) according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2023Quote: ... 5’-capped mRNA encoding the HpCTCF-GFP fusion protein was synthesized using the mMESSAGE mMACHINE™ T7 Trinscription Kit (Ambion, USA) and purified by phenol/chloroform extraction and isopropanol precipitation ...
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bioRxiv - Microbiology 2023Quote: ... The azide group of ADA-DA was labelled (post-fixation) with 5 µg ml−1 Alexa Fluor 488 Alkyne using the Click-iT™ Cell Reaction Buffer Kit (Invitrogen) according to the manufacturer instructions.
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bioRxiv - Developmental Biology 2023Quote: ... NAE staining was followed by 5-ethynyl-2⍰-deoxyuridine (EdU) detection using Click-iT Edu Alexa Fluor 488 or 594 Imaging kits (Life Technologies) according to the manufacturer’s instructions.
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bioRxiv - Developmental Biology 2023Quote: ... and sequenced with the M13 forward primer (5’-GTAAAACGACGGCCAG-3’) using a BigDye Terminator v3.1 Cycle Sequencing Kit (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2023Quote: ... OCI-Ly7 and SU-DHL-5 were authenticated by DNA barcoding using the sensitive AmpFISTR Identifiler PCR amplification kit (Applied Biosystems). The amplified products were analysed by capillary electrophoresis (Eurofins Medigenomix GmbH ...
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bioRxiv - Developmental Biology 2023Quote: ... 0.5 μg RNA from each sample was used for reverse transcription with a RevertAid First Strand cDNA Synthesis Kit (Thermo scientific). qPCR was performed with ChamQ Universal SYBR qPCR Master Mix (Vazyme ...
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bioRxiv - Systems Biology 2023Quote: 5 ng total RNA from 3D or overlay cultures was used with the TaqMan MicroRNA Reverse Transcription Kit (Thermo Fisher, 4366596) and TaqMan MicroRNA Assays (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... The nuclear extract was then spun at 21,000g for 5 minutes and the supernatant recovered and biotinylated using an EZ-Link Sulfo-NHS-LC-Biotin kit (Thermo Scientific). The solution was dialyzed using Slide-A-Lyzer® Dialysis Cassette G2 (cat# 87724 ...
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bioRxiv - Neuroscience 2023Quote: ... 5’-GGAGCCATGGCCGCGTCCGG-3’ and 5’-GGACGGGCGGTCGGTCTGAG-3’ were chosen as Cas9 targeted guide RNAs (sgRNAs) and in vitro transcribed using the MEGAshortscript Kit (ThermoFisher, USA) and subsequently purified using MEGAclearTM Kit ...
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bioRxiv - Microbiology 2023Quote: ... The CHIKV genome was amplified in 5 PCR fragments using Phusion High-Fidelity PCR Master Mix Kit with HF Buffer (Thermo Scientific). CHIKV fragments were confirmed by an agarose gel and sent to Sanger Sequencing using the primers in Table 1 for confirmation of mutations in all working CHIKV viral stock mutations (Genewiz).
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bioRxiv - Biophysics 2023Quote: ... cells were cultured and transiently co-transfected with wild-type or mutant Nav1.2 channel variant (5 μg) and enhanced green fluorescent protein (1 μg) using Lipofectamine 3000 kit (Thermo Fisher Scientific), as previously described (26) ...
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bioRxiv - Plant Biology 2024Quote: 5 μg of total RNA were first depleted of rRNA with the RiboMinus Plant Kit for RNA-Seq (Thermo Fisher Scientific). RNA was de-capped using Cap-Clip Pyrophosphatase ...
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bioRxiv - Immunology 2024Quote: ... For T cell panels (Supplemental table 2, 4, 5, 6) we fixed cells with the FOXP3 Fixation/Permeabilization Buffer Kit (Thermo Fisher) and conducted intracellular/nuclear stains using the FOXP3 Permeabilization Buffer (Thermo Fisher ...
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bioRxiv - Cell Biology 2024Quote: ... G cap on its 5’ terminal and poly (A) tail on its 3’ terminal using the mMESSAGE mMACHINE T7 Ultra Kit (Life Technologies). Both the Cas9 mRNA and the sgRNAs were purified using the MEGAclear Kit (Life Technologies ...
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bioRxiv - Microbiology 2024Quote: ... The dry pooled sample was resuspended in 1 ml of 5% formic acid and was desalted using the EasyPep Maxi Kit (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... MHV68-MR-infected, or MHV68-R443I-infected MC57G cells (MOI=5, 24 h) in biological triplicate using miRVANA isolation kits (Thermo Fisher) and was used for generating OTTR-seq libraries20 ...
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bioRxiv - Microbiology 2024Quote: ... qRT-PCR was performed using KAPA SYBR fast universal qPCR kit on QuantStudio™ 5 System (Applied Biosystems, Waltham, MA, USA). The reactions were performed in triplicate ...
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bioRxiv - Cell Biology 2022Quote: ... images were collected from the ProteinSimple FluorChem system (protein simple, Fluorchem M FM0561) after incubation with SuperSignal West Pico PLUS Chemiluminescent Substrate (Thermo Fisher Scientific, Cat. No. 34580). All antibody information is listed in Supplementary Table 3.
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bioRxiv - Cell Biology 2024Quote: ... the protein signals were captured by chemiluminescent substrates (SuperSignal™ West Pico or Extended Dura or Femto Maximum Sensitivity Substrate, Thermo Fisher Scientific, MA, USA) and the blot image was acquired using Bio-Rad ChemiDoc MP Imaging System (12003154 ...
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bioRxiv - Cancer Biology 2023Quote: ... and chemiluminescent HRP substrates (Supersignal West Pico PLUS, Thermo Fisher Scientific; Pierce ECL Plus, Thermo Fisher Scientific; or Immobilon ECL Ultra, Millipore) (Thermo Fisher Scientific, RRID:SCR_008452).
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bioRxiv - Cancer Biology 2023Quote: ... After three washes with TBS-T to eliminate the primary antibody and immunoreacting bands revealed using a standard chemiluminescent method (Thermo Fisher Scientific, Catalog No. 32106).
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bioRxiv - Cell Biology 2019Quote: ... 96-well glass-bottom plates (Thermo Scientific) 48 hours before imaging ...
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bioRxiv - Immunology 2021Quote: Nunc Immuno 96 well plates (Fisher Scientific) were coated with 1μg/ml (50 μl/well ...
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bioRxiv - Immunology 2020Quote: ... 96-well plates (Thermo Fisher Scientific, USA) were coated per well with 250 ng of individual recombinant proteins ...
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bioRxiv - Molecular Biology 2021Quote: ... protein adsorbing 384-well microtiter plates (Nunc® MaxisorpTM ...
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bioRxiv - Molecular Biology 2020Quote: ... Plates were sealed (Thermo Fisher Scientific #AB0626) immediately spun down and frozen at −80 °C until further processing.
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bioRxiv - Immunology 2022Quote: ... 96-well MaxiSorp plates (Thermo Scientific #442404) were coated with 50 μL/well of recombinant SARS-CoV-2 S1 protein (ACRO Biosystems S1N-C52H3 ...
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bioRxiv - Microbiology 2022Quote: ... ProcartaPlex magnetic bead cytokine/chemokine plates (Invitrogen) were prepared according to the manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2020Quote: ... on six-well Nunclon surface plates (Nunc) coated with Geltrex (ThermoFisher Scientific ...
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bioRxiv - Molecular Biology 2021Quote: ... were grown in feeder-free conditions in Matrigel (Corning)-coated plates in StemFlex medium (Thermo Fisher). To produce organoids ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... Black 96-well plates (Thermo Scientific, #7805) were coated to dry overnight at 37°C with 400 ng/well of recombinant His-tagged GT198 proteins together with 5 μg/well of purified BSA (NEB ...
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bioRxiv - Immunology 2019Quote: ... 96-well Nunc MaxiSORP plates (Thermo Scientific) were pre-coated with methylated BSA (Sigma ...
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bioRxiv - Systems Biology 2020Quote: ... 96-well PCR plates (AB0600, Thermo Scientific) were prepared with 3.52 (4.4 ...
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bioRxiv - Neuroscience 2019Quote: ... in 6-well culture plates (ThermoFisher Scientific) with 800 µL of sterile medium added below the insert (Stoppini et al. ...
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bioRxiv - Bioengineering 2020Quote: ... Medisorp 96-well plates (Thermo Fisher Scientific) were coated overnight at 4 °C with 10 μg/mL protective antigen in PBS ...
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bioRxiv - Cell Biology 2020Quote: ... in 96-well PCR plates (Applied Biosystems). FAM/MGB-NFQ fluorescence emissions were monitored after each cycle ...
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bioRxiv - Cell Biology 2020Quote: ... in 96-well PCR plates (Applied Biosystems). SYBR Green or FAM/BHQ-1 fluorescence emissions were monitored after each cycle ...
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bioRxiv - Immunology 2020Quote: ... 96 well flat-bottomed plates (Nunc, #439454) were coated with Sm/RNP (ImmunoVision ...
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bioRxiv - Immunology 2020Quote: ... 96 well flat-bottomed plates (Nunc, #439454) were coated with anti-IgG-FC overnight at room temperature ...
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bioRxiv - Genetics 2019Quote: ... low-binding culture plate (Thermo Fisher Scientific) for 2 days in GMEM supplemented with 15% Knockout serum replacement (Invitrogen) ...
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bioRxiv - Cell Biology 2019Quote: ... 4X 14 cm plates (Nunc, Roskilde, Denmark) of HUVEC were required for each proteomics condition analysed ...