Labshake search
Citations for Thermo Fisher :
4051 - 4100 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2023Quote: ... and then starved in 50 µl EMEM-Serum free medium (antibiotic free) in presence or absence of a protein kinase inhibitor (PKI) (6-22amide, 50 nM, GIBCO BRL) for 3 hours ...
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bioRxiv - Neuroscience 2023Quote: ... The injected individuals were kept at 18°C for 5 to 8 days in 6-well-plates (Nunc multidish no. 150239, Thermo Scientific) and then cultured at 22°C until sexual maturity ...
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bioRxiv - Microbiology 2023Quote: Primary human macrophages were generated by differentiating blood-derived CD14+ monocytes for 6 days in Roswell Park Memorial Institute (RPMI)-1640 medium (ThermoFisher Scientific) containing 10% HyClone FBS (Cytiva ...
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bioRxiv - Immunology 2023Quote: ... cDNA from samples was mixed with QuantiTect probe PCR master mix and gene-specific primers to run on a QuantStudio 6 Flex real-time cycler (Applied Biosystems). To generate a control for the amplification reaction ...
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bioRxiv - Bioengineering 2023Quote: ... reprogramming was continued in E8 medium and from Day 6 to Day 18 in E7 medium (E6 medium, cat. A1516401, Thermo Fisher Scientific + 100ng ml-1 bFGF ...
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bioRxiv - Biochemistry 2023Quote: ... 2 µg of plasmid and 6 µg of PEI were diluted in 0.5 ml of Opti-MEM™ Reduced serum medium (Gibco™) per single well ...
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bioRxiv - Cell Biology 2022Quote: ... 2 μg of DNA and 6 μg of PEI were diluted in 0.25 ml of Opti-MEM™ Reduced serum medium (Gibco™), incubated for 30 minutes at room temperature and added dropwise to plates containing cells ...
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bioRxiv - Bioengineering 2023Quote: HEK293 cells were seeded on a 6-well plate (2×105 cells per well) and were cultured in low glucose Dulbecco’s Modified Eagle Medium (Thermo Fisher Scientific) supplemented with 10% FBS and 1% sodium pyruvate ...
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bioRxiv - Bioengineering 2023Quote: ... the hydrogel is first activated with sulfosuccinimidyl 6-(4′-azido-2′-nitrophenylamino) hexanoate (Sulfo-SANPAH, Pierce, Thermo Fisher Scientific, Waltham, MA). Here the remaining washing buffer around the gel edge is removed to ensure the coating solution can be held on top of the gel ...
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bioRxiv - Cancer Biology 2023Quote: ... 2.6 µm particle size column mounted in a Vanquish LC coupled to an Orbitrap ID-X Tribrid mass spectrometer (ThermoFisher Scientific). The mobile phases and chromatographic gradients used are described in Supplementary Table S6 ...
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bioRxiv - Developmental Biology 2023Quote: ... Antigen unmasking was performed by microwaving the slides for 2 × 10 min in citrate buffer solution (pH 6) (Thermo Fisher Scientific). Sections were then blocked for 1 hour at room temperature with 10% normal donkey serum (Chemicon International Inc. ...
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bioRxiv - Cancer Biology 2023Quote: ... 30 ng of cDNA was used to perform the qRT-PCR reaction using SYBR green PCR master mix on an ABI Quant Studio 6 Flex Real-Time PCR System (Applied Biosystems). The housekeeping genes GAPDH ...
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bioRxiv - Cell Biology 2023Quote: ... and coverslips were mounted using Prolong Gold Antifade Reagent with 4′,6-diamidino-2-phenylindole (DAPI) (Life Technologies, Carlsbad, CA, USA). Cells were viewed using an Olympus IX81 or Olympus BX53 epifluorescence microscope ...
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bioRxiv - Cancer Biology 2023Quote: ... 1.5 x 106 KELLY or 5 x 105 TET21N cells were transfected on a 6-well plate using 7.5 µl Lipofectamine RNAiMAX (Thermo Fisher Scientific) and 50 pmol siRNA according to the manufactureŕs instructions ...
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bioRxiv - Cell Biology 2023Quote: ... transfected cells are cultured for 3 days in a 6-well plate before they are gently detached with 10-min incubation in an enzyme-free cell dissociation buffer (Gibco, 13151014). These detached cells are then replated on ECM-coated substrates and imaged after 6 hrs.
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bioRxiv - Immunology 2023Quote: Spleen cells from CD45.1+ CD45.2- male and CD45.1- CD45.2+ female C57BL/6 wild type mice were harvested as described above and stained with Cell Trace Violet cell proliferation kit (Molecular Probes) in PBS at different concentration ...
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bioRxiv - Cell Biology 2023Quote: ... Real-time PCR was performed in MicroAmp optical 384-well plates using the QuantStudio 6 Flex RT-PCR system (Thermo Fisher). The default thermocycling program was used (95ºC for 10 min ...
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bioRxiv - Developmental Biology 2022Quote: Cell sizes of mouse and rat embryonic molars were determined by staining 6 µm thick histological sections with DiI (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2023Quote: ... washed 1x with PBS and plated at 200,000 cells per well in 6-well plates coated with matrigel in iMGL media [DMEM/F12 (Thermo Fisher Scientific), ITS-G (2% v/v ...
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bioRxiv - Biochemistry 2023Quote: ... Sf9 cells were seeded at 5·105 cells/ml in a 6-well plate in a total volume of 2 ml of Sf-900-II SFM media (10902088, ThermoFisher Scientific). Bacmids (see above ...
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bioRxiv - Biochemistry 2023Quote: ... cells from 100 mL of culture were resuspended and chemically lysed for 30 min in 6 mL of B-PER II (Thermo Fisher) diluted to 1x with TEMB buffer (10 mM Tris pH 8.0 ...
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bioRxiv - Developmental Biology 2023Quote: ... jaw specimen undergone silver deposition and gold enhancement was casted in 6 % low melting point agarose (TopVision, ref R0801, Thermo Scientific) in 1xPBS and sectioned to 100 µm sections with vibratome (Microm) ...
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bioRxiv - Genomics 2022Quote: ... two-thirds of the cells were collected for single-cell sequencing and the remaining cells were re-plated on 6-well plates coated with 5 μg/cm2 Type I rat collagen (Gibco, A1048301). Two additional samples were collected on Days 11 and 21 for single-cell sequencing ...
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bioRxiv - Neuroscience 2023Quote: ... Quantitative PCR (qPCR) was completed using the SYBR green method carried out on a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems) using LabTaq Green Hi Rox (Labtech) ...
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bioRxiv - Bioengineering 2023Quote: ... sections were counterstained with 1 mg/mL of 4’,6-diamidino-2-phenylindole (DAPI) and 0.1 nmol units/ml of Phalloidin ATTO647N and mounted with Fluoromount-G (Thermo Fisher Scientific). The slides were acquired using a confocal (Leica SP8 ...
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bioRxiv - Molecular Biology 2023Quote: ... and size exclusion chromatography (SEC) using a Superose 6 10/300 GL Increase column (Cytiva, MA) equilibrated in PBS buffer (Thermo Scientific 137mM NaCl ...
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bioRxiv - Neuroscience 2023Quote: ... The virus was propagated in C6/36 Aedes albopictus cell line at a multiplicity of infection (MOI) of 0.01 and cultured for 6 days in Leibovitz’s L-15 medium (Thermo Fisher Scientific, 11415064) supplemented with 0.3% tryptose phosphate broth (Sigma-Aldrich ...
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bioRxiv - Neuroscience 2023Quote: ... including Camuiα were transfected into HEK293S GnTI-cells (2 x 106 cells per well in 6-well plates) cultured in FreeStyle 293 (Thermo Fisher), using the TransIT2020 transfection reagent (Mirus Bio) ...
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bioRxiv - Molecular Biology 2023Quote: ... After 3 washes cells were stained with a combination of Alexa Fluor secondary antibodies and 4′,6-Diamidino-2-Phenylindole (DAPI; Molecular Probes) for 1 h at room temperature in the dark ...
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bioRxiv - Plant Biology 2023Quote: ... The RT-qPCR was performed using the ChamQ Universal qPCR Master Mix (Vazyme Biotech) on the QuantStudioTM 6 Flex Real-Time PCR System (Applied Biosystems). The relative transcript level was calculated by the ΔΔCT comparative quantification method (50 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... The 6-well plates with prepared corneas were placed on a rocker platform (Figure 40) inside a CO2 incubator (Heracell, Thermo Fisher) and incubated with 5% CO2 at 37°C.
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bioRxiv - Neuroscience 2023Quote: ... Neurons (100000 cells/ well of a 6-well) were plated onto poly-L-lysine-coated coverslips and grown in modified Eagle medium (MEM; Thermo Fisher) supplemented with 5% FCS and 2% B-27 (Gibco ...
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bioRxiv - Cancer Biology 2023Quote: ... a dry transfer was completed at 30V for 6 min with PVDF stacks in an iBlot 2 transfer instrument (Thermo Fisher). After gel transfer ...
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bioRxiv - Cell Biology 2023Quote: ... and ProLong™ Gold antifade reagent with 4′,6-diamidino-2-phenylindole (DAPI) (P36941) or without (P36930) was purchased from Invitrogen.
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bioRxiv - Cancer Biology 2023Quote: ... 3 µL of primers (final concentration 200 nM) and 6 µL of Power SYBR Green PCR Master Mix (Thermo Fisher Scientific). The amplification conditions included an initial incubation for 2 min at 50°C ...
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bioRxiv - Molecular Biology 2023Quote: ... qRT PCR was performed as described previously (Li et al, 2018) using QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). SsoFast EvaGreen Supermixe (Takara ...
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bioRxiv - Neuroscience 2023Quote: Aggregates from four wells of a 6-well plate were transferred to one 175 cm2 tissue culture flask (Thermo Scientific, #159926) previously treated with an anti-adherence solution (StemCell Technologies ...
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bioRxiv - Cell Biology 2023Quote: Rat RFL-6 fibroblasts were purchased from the American Type Culture Collection (ATCC) and cultured in Ham’s F-12K medium (Thermo Fisher Scientific) supplemented with 20% fetal bovine serum and 1% pen-strep ...
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bioRxiv - Genomics 2023Quote: ... the fibroblasts were thawed and plated at a density of 250K cells/well of 6-well plate and infected with the Cytotune Sendai virus (Life Technologies) per manufacturer’s protocol to initiate reprogramming ...
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bioRxiv - Molecular Biology 2023Quote: ... Grids were blotted for 6 s with a blotting force of 6 at 4 °C and 100% humidity and plunged into liquid ethane using a FEI Vitrobot Mark IV (Thermo Fisher). The data were collected with a Glacios Cryo-TEM (Thermo Fisher ...
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bioRxiv - Genetics 2023Quote: Total RNA was isolated from the individual larval zebrafish and 6-month-old adult brain samples using the mirVana™ miRNA Isolation Kit (Invitrogen) following the manufacturer’s instructions ...
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bioRxiv - Genomics 2023Quote: ... The expression of mRNA was examined by quantitative PCR analysis using a QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). TaqMan assays were used to quantitate ...
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bioRxiv - Microbiology 2023Quote: All transfections were performed on 293T cells (seeded at a density of 0.5 × 106 cells/well of a 6-well plate one day prior) using Lipofectamine 3000 transfection reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendation unless stated otherwise ...
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bioRxiv - Cancer Biology 2023Quote: ... TRAIL blocking ab was purchased from Santa Cruz TRAIL Antibody (RIK-2): sc-56246, and IFN gamma blocking Monoclonal Antibody (NIB42, #6-7318-81) was purchased from Thermo Fisher. The blocking mAb was added when NK cells were combined with tumor cells.
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bioRxiv - Cell Biology 2023Quote: ... 10 μg total RNA was denatured in formamide loading dye and resolved on the 6% TBE-Urea gel (Novex, ThermoFisher Scientific) in 1 X TBE buffer ...
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bioRxiv - Cell Biology 2023Quote: ... on non-tissue-culture treated 6-well plates that were pre-treated overnight with PBS + 0.1 mg/ml Poly-D-lysine (Thermo Scientific, A3890401) and 200 μg/ml human JAGGED1-Fc ...
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bioRxiv - Developmental Biology 2023Quote: ... iPSCs were dissociated using Accutase and seeded at a density of 5.5 x106 cells per well in ultra-low attachment 6-well plates (Thermo Fisher Scientific) in E8 medium supplemented with 10 μM ROCK inhibitor ...
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bioRxiv - Cell Biology 2023Quote: ... UNC45A-GFP overexpressing RFL-6 cells (16 hrs post-infection) were treated with Tubulin Tracker Deep Red (Thermo Fisher Scientific T34077) and imaged as we have previously described [13] ...
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bioRxiv - Bioengineering 2023Quote: ... Hydrogels were washed extensively with PBS and activated via photoirradiation (365 nm, 0.8 mW for 5 minutes) with sulfosuccinimidyl-6-4’-azido-2’-nitrophenylamino hexanoate (Sulfo-SANPAH; Thermo Scientific Pierce). To do so ...
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bioRxiv - Cell Biology 2023Quote: ... Same cDNA was used to amplify both the gene of interest and 18S rRNA in Quant-Studio 6 Flex real-time PCR system (Applied Biosystems). The PCR conditions consisted of AmpliTaq Gold activation at 95°C for 10 min ...