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Citations for Thermo Fisher :
4101 - 4150 of 10000+ citations for 6 fluorohexane 1 sulfonyl fluoride since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... 3×105 cells were seeded in a 6-well plate and transfected 24h later with 5 µg pX459 plasmid DNA using Lipofectamine 3000 (Thermo Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2023Quote: ... The isolated primary mouse hepatocytes were plated onto collagen-coated 6-well plates at a density of 500,000 cells/well in 4 ml of DMEM (Thermo Fisher Scientific) plus 10% fetal bovine serum ...
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bioRxiv - Neuroscience 2023Quote: ... on day -2 fibroblasts were seeded in increasing concentrations on two 6-well plates (10-35 x 104 cells/well) with reprogramming fibroblast medium (PFM; DMEM, 10% ESC-qualified FBS (Thermo Fisher), 1% NEAA (Thermo Fisher) ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were plated at a density of 1.5 × 10^5 per well on 6-well plates in Neurobasal media (Gibco 21103-049) containing B27 supplement (Gibco 17504-044) ...
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bioRxiv - Neuroscience 2023Quote: ... BIHi005-A (male) and BIHi250-A (female) were cultured on 6 well-plates coated with hESC-qualified Geltrex (Thermo Fisher, #A1413302) in E8 Medium (Thermo Fisher ...
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bioRxiv - Neuroscience 2024Quote: ... differentiated OLs at 6 DIV (‘OL 3 days’) were transfected with plasmids using Lipofectamine LTX with Plus Reagent (Thermo Fisher Scientific) according to the manufacturer’s recommendations ...
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bioRxiv - Bioengineering 2024Quote: ... 3D iHeps (Day 22) were incubated for 2 hours with HCM media containing 1μmol/L of 6-CDF (Thermofisher, #C-369). Samples were then washed with PBS for 15 minutes before staining with Hoescht in HCM.
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bioRxiv - Biophysics 2024Quote: ... The peptides above were redissolved in 25 μL 0.1% TFA and 6 μL of them were analyzed by Orbitrap Exploris 480 mass spectrometer (Thermo Fisher Scientific).
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bioRxiv - Cancer Biology 2024Quote: ... RIPA Lysis and Extraction Buffer (CAT NO. 89901), and DAPI (4’,6-Diamidino-2-Phenylindole, Dilactate) (CAT NO. D3571) were purchased from Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2024Quote: ... The reactions were separated on a 6% precast DNA retardation gel in 0.5% Tris borate-EDTA buffer (TBE, Thermo Fisher Scientific) at 100 V for 1.5 hours ...
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bioRxiv - Evolutionary Biology 2024Quote: ... washing was performed again but cells were resuspended in 12mL of HNM+80μg/mL and plated across a 6-well plate (Thermo Scientific; 140685). Cells were then incubated for 72 hours at 22oC and 60% humidity ...
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bioRxiv - Cancer Biology 2024Quote: The proteomics samples consisting of 24 fractions for the proteome analysis and 6 fractions for the phosphoproteome analysis were analyzed by LC/MS/MS using an Ultimate 3000-Exploris480 (Thermo Scientific). Samples were injected onto an IonOpticks Aurora series 2 C18 column (75 μm id × 15 cm ...
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bioRxiv - Cancer Biology 2024Quote: ... CHLA-42 cells were seeded in 6 well plates and transfected with 25pmol H19 siRNAs according to Lipofectamine ™ RNAiMAX transfection reagent (Invitrogen) manufacturer protocol ...
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bioRxiv - Neuroscience 2024Quote: ... iPSCs were plated at 100% density in 6-well plates in NMM medium (50% DMEM/F-12 + GlutaMAX (Thermo Fisher, 31331093), 50% NBM ...
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bioRxiv - Biophysics 2024Quote: ... grids were blotted once for 2.5 – 6 s (4 °C and 100 % humidity) using a FEI Vitrobot Mark IV (Thermo Fisher Scientific) and plunge-frozen in liquid ethane ...
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bioRxiv - Cancer Biology 2024Quote: ... and KiCqStart pre-designed SYBR green gene-specific primers (Supplementary Table S4) in QuantStudio 6 Flex Real-Time PCR System (Applied Biosystems). Expression level for each gene was normalized to GAPDH for each experiment ...
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bioRxiv - Microbiology 2024Quote: ... 2X105 cells were seeded into a 6-well plate and transfected using a total of 2 µg of plasmid using Lipofectamine 3000 (Invitrogen, USA) as per the manufacturer’s instruction ...
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bioRxiv - Genomics 2024Quote: 20 μg of retroviral plasmid DNA (sgGFP-Cr2 or sgZbtb20-Cr3-cs2) with 6 μg pCL-Eco plasmid DNA were transfected using 60 μl Lipofectamine 2000 (Invitrogen, #1668019) in 3 ml OptiMEM (Invitrogen ...
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bioRxiv - Biochemistry 2024Quote: Scaffolds or 6 mm punches of dissected fresh mouse cortical brain tissue were placed on a Fisherbrand double frosted microscope slide (Fisher Scientific) in the center of an adhesive ...
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bioRxiv - Molecular Biology 2024Quote: ... coverslips were mounted on glass slides using ProLong Gold antifade (4′,6-diamidino-2-phenylindole) DAPI to counterstain the nuclei (Thermofisher, #P36935).
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bioRxiv - Immunology 2024Quote: ... All samples were assayed using the 7300HT or QuantStudio 6 Fast Real-Time PCR System and analyzed using Fast System Software (Applied Biosystems). All real-time PCR data was normalized to the level of Gapdh mRNA ...
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bioRxiv - Molecular Biology 2024Quote: Wild-type mESCs derived from blastocysts of mixed 129-C57Bl/6 background were cultured in media containing DMEM (Gibco, 31966-021), supplemented with LIF (in-house production) ...
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bioRxiv - Microbiology 2024Quote: TR146 cells grown in antibiotics-free media at ∼70% confluence in 6-well plate were transformed with lipofectamine RNAiMAX reagent (Invitrogen, #13778) according to the manufacture’s protocol ...
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bioRxiv - Microbiology 2024Quote: RNA was collected from mmIFNα1 treated or untreated cells grown in 6-well plates 17 hours after treatment using TRIzol (Thermo Fisher Scientific). cDNA was then generated using SuperScript III reverse transcriptase and oligo-dT primers (Invitrogen) ...
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bioRxiv - Molecular Biology 2024Quote: ... patient-derived immortalized myoblasts and non-DUX4 expressing cells (54-6; 13 D4Z4 repeat units) were grown in Ham’s F-10 Nutrient Mix (Gibco, Waltham, MA) with 20% FBS (Corning ...
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bioRxiv - Molecular Biology 2024Quote: ... Serum IL-6 and TNF-α concentrations were determined with enzyme-linked immunosorbent assay (ELISA) kits (Legend Max Mouse IL-6 ELISA Kit, BioLegend, and Mouse TNF Alpha Uncoated ELISA, Invitrogen, respectively). Serum noradrenaline was extracted with the use of a cis-diol–specific affinity gel ...
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bioRxiv - Molecular Biology 2024Quote: ... or 2.5 µg (6-well plates) of plasmid DNA diluted in OptiMEM medium using the Lipofectamine 3000 reagent (Invitrogen, ThermoFisher Scientific, France) for 48 h ...
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bioRxiv - Genomics 2024Quote: ... iPSCs were plated at high density (2 million cells/well in a 6 well plate) on hESC-qualified Geltrex (Gibco #A1413302), then cultured in neural induction media at high density with ROCK inhibitor Y27632 (StemCell Tech ...
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bioRxiv - Immunology 2024Quote: ... For T cell panels (Supplemental table 2, 4, 5, 6) we fixed cells with the FOXP3 Fixation/Permeabilization Buffer Kit (Thermo Fisher) and conducted intracellular/nuclear stains using the FOXP3 Permeabilization Buffer (Thermo Fisher ...
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bioRxiv - Genomics 2024Quote: ... Samples were further incubated at 95°C for 10 minutes in a thermoblock and loaded on a 6% TBE-Urea acrylamide denaturing gel (Thermo Scientific). Gels were stained with SYBR-gold (1:10000 in 1x TBE ...
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bioRxiv - Genomics 2024Quote: ... Resulting mRNA was split into 6 separate reactions and cDNA was synthesized using a gene-specific primer (5’-CTCATCAATGTATCTTATCATGTCTG-3’) and MultiScribe Reverse Transcriptase (Invitrogen, 4311235). Following cDNA synthesis ...
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bioRxiv - Cell Biology 2024Quote: ... 2.5×10^5 cells per well were cultured overnight in a 6-well plate and surface labeled with NHS-LC biotin (Thermofisher#21335) at a concentration of 0.2 mg/ml in PBS for 20 minutes on ice ...
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bioRxiv - Developmental Biology 2024Quote: ... extracted from a single well of a 6-well plate using a mixture of RIPA lysis buffer and protease inhibitor then quantified using Pierce BCA kit (ThermoFisher Scientific), were separated via SDS-PAGE and subsequently transferred onto PVDF membranes ...
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bioRxiv - Bioengineering 2024Quote: ... the scalp was shaved before each session and the transducer affixed to the animal’s head via implanted titanium pins (Gray Matter Research, USA) and coupled using a 6% polyvinyl alcohol (Fisher Scientific, USA) cryogel ...
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bioRxiv - Molecular Biology 2024Quote: ... The medium was adjusted to pH 5.8 using 1N KOH prior to addition of 6 g/L Phytagar (Gibco, Life Technologies, U.S.A) and autoclaving for 20 min at 121°C ...
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bioRxiv - Biophysics 2021Quote: ... the soluble fraction was filtered and incubated for 3 h at 4 °C with ∼6 ml of HisPurTM Ni-NTA resin (Thermo Fisher Scientific). The beads were subsequently washed with ∼400 ml lysis buffer (without protease inhibitors or Arginine/Glutamic acid ...
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bioRxiv - Cancer Biology 2021Quote: 143B cells were plated into a 6-well plate and transfected with 100 nM of miR-34c mimic or negative miRIDIAN mimic using Lipofectamine 2000 (Thermo Fisher Scientific) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2020Quote: ... The protein content of preovulatory follicles from eCG-injected mice is ~6 μg (Pierce BCA protein assay kit, 23225, Thermo Fisher Scientific). The protein content of follicles (~320-400 μm diameter ...
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bioRxiv - Neuroscience 2020Quote: ... Fish were euthanized 6 hours post-treatment and Biocytin was histologically detected using Alexa Fluor 594-conjugated Streptavidin (Molecular Probes, S-11227). Biocytin-labeled axons were quantified using the “threshold” and “particle analysis” tools in the ImageJ software (26) ...
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bioRxiv - Cell Biology 2020Quote: ... A gradient consisting of 0% to 40% B in A (A: 0.1% formic acid [Fisher Scientific, A117], 6% acetonitrile [Fisher Scientific, A955], in H2O [Fisher Scientific, W6] ...
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bioRxiv - Microbiology 2020Quote: ... trachomatis-infected cells by scraping one or two wells of a 6-well tissue culture plate in a total volume of 500 μL Trizol Reagent (Thermo Fisher Scientific). Samples were transferred to RNase-free o-ring capped tubes containing ~100 μL volume of zirconia beads and thoroughly vortexed for 10 min to rupture bacterial cells ...
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bioRxiv - Microbiology 2019Quote: Spotted protein arrays (5 x 6) were printed onto each well of Greiner high-binding 96-well plates (Cat# 655097, Thermo Fisher Scientific) using a sciFLeXARRAYER S12 (Scienion AG ...
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bioRxiv - Molecular Biology 2021Quote: For DNA plasmids: 5×106 HEK293T cells were grown in 6-well plates and Lipofectamine™ 3000 Transfection Reagent (L3000001, ThermoFisher UK) was used to transfect the cells following the manufacturer’s instructions ...
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bioRxiv - Molecular Biology 2021Quote: ... and a human IL-6 siRNA pool (Horizon Discovery, LQ-007993-00-0005) were transfected using Lipofectamine™ RNAiMAX Transfection Reagent (Invitrogen, 13778) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2021Quote: ... for anti-eEF3 and anti-G-6-PDH secondary antibodies and developed with Pierce ECL plus western blotting substrate kit (Thermo Scientific 32132).
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bioRxiv - Molecular Biology 2021Quote: ... The PCR products detected on 6% polyacrylamide or 2% agarose gels were purified with a Charge Switch PCR Clean-Up kit (Invitrogen, Carlsbad, CA) and then sent to BMR genomics (Padova ...
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bioRxiv - Immunology 2020Quote: ... The peptide was conjugated directly to surface lysines on Qβ bacteriophage VLPs using the bidirectional crosslinker succinimidyl 6-[(beta-maleimidopropionamido) hexanoate] (SMPH; Thermo Fisher Scientific) as previously described 47 ...
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bioRxiv - Developmental Biology 2020Quote: ... 2×105 cells/well (6-well plate) HCECs were transfected with 30pmol siRNA using lipofectamine RNAiMax (Life Technologies, Grand Island, NY, USA) according to the manufacturer’s protocol ...
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bioRxiv - Genomics 2020Quote: Exon 2 of A3A and exons 5 and 6 of A3B with either 20 or 80 bp of flanking intronic sequences were synthesized (Thermo Fisher Scientific) and cloned in sense orientation using XhoI and NotI restriction sites of Exontrap vector pET01 (MoBiTec ...
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bioRxiv - Molecular Biology 2022Quote: ... PCR amplicons were detected by electrophoresis on 6% non-denaturing polyacrylamide/TBE gels with SYBR Gold Nucleic Acid Gel Stain (Thermo Fisher Scientific). Splicing efficiency was measured by densitometry analysis of the PCR amplicons in image J (Schindelin et al. ...